Experiment: MoLS4 with L-Cysteine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysD and DVU1567 are separated by 9 nucleotides DVU1567 and ftn are separated by 163 nucleotides ftn and porA are separated by 72 nucleotides
DVU1566: cysD - phosphoadenosine phosphosulfate reductase, putative (TIGR), at 1,649,064 to 1,649,738
cysD
DVU1567: DVU1567 - hypothetical protein (TIGR), at 1,649,748 to 1,649,960
DVU1567
DVU1568: ftn - ferritin (TIGR), at 1,650,124 to 1,650,633
ftn
DVU1569: porA - pyruvate ferredoxin oxidoreductase, alpha subunit (TIGR), at 1,650,706 to 1,652,442
porA
Position (kb)
1650
1651 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1649.150 kb on + strand, within cysD at 1649.196 kb on - strand, within cysD at 1649.196 kb on - strand, within cysD at 1649.298 kb on + strand, within cysD at 1649.306 kb on - strand, within cysD at 1649.344 kb on + strand, within cysD at 1649.356 kb on - strand, within cysD at 1649.361 kb on - strand, within cysD at 1649.471 kb on + strand, within cysD at 1649.471 kb on + strand, within cysD at 1649.615 kb on + strand, within cysD at 1649.632 kb on + strand, within cysD at 1649.639 kb on + strand, within cysD at 1649.639 kb on + strand, within cysD at 1649.640 kb on + strand, within cysD at 1649.650 kb on + strand, within cysD at 1649.745 kb on + strand at 1649.745 kb on + strand at 1649.747 kb on + strand at 1649.753 kb on - strand at 1649.769 kb on - strand at 1649.883 kb on + strand, within DVU1567 at 1649.978 kb on - strand at 1649.978 kb on - strand at 1649.994 kb on + strand at 1650.002 kb on - strand at 1650.026 kb on - strand at 1650.060 kb on + strand at 1650.060 kb on + strand at 1650.068 kb on - strand at 1650.170 kb on - strand at 1650.328 kb on + strand, within ftn at 1650.338 kb on - strand, within ftn at 1650.338 kb on - strand, within ftn at 1650.383 kb on - strand, within ftn at 1650.386 kb on + strand, within ftn at 1650.408 kb on + strand, within ftn at 1650.512 kb on - strand, within ftn at 1650.512 kb on - strand, within ftn at 1650.517 kb on - strand, within ftn at 1650.578 kb on - strand, within ftn at 1650.595 kb on - strand at 1650.685 kb on + strand at 1650.685 kb on + strand at 1650.788 kb on + strand at 1650.788 kb on + strand at 1650.788 kb on + strand at 1650.788 kb on + strand at 1650.796 kb on - strand at 1650.796 kb on - strand at 1650.833 kb on - strand at 1650.852 kb on + strand at 1650.858 kb on + strand at 1650.898 kb on + strand, within porA at 1650.926 kb on + strand, within porA at 1650.991 kb on - strand, within porA at 1651.011 kb on + strand, within porA at 1651.186 kb on - strand, within porA at 1651.194 kb on - strand, within porA at 1651.291 kb on - strand, within porA at 1651.292 kb on - strand, within porA at 1651.298 kb on - strand, within porA at 1651.392 kb on + strand, within porA at 1651.400 kb on - strand, within porA at 1651.400 kb on - strand, within porA at 1651.490 kb on - strand, within porA at 1651.621 kb on + strand, within porA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM remove 1,649,150 + cysD DVU1566 0.13 -0.1 1,649,196 - cysD DVU1566 0.20 -1.1 1,649,196 - cysD DVU1566 0.20 -2.3 1,649,298 + cysD DVU1566 0.35 +3.1 1,649,306 - cysD DVU1566 0.36 -0.7 1,649,344 + cysD DVU1566 0.41 +0.5 1,649,356 - cysD DVU1566 0.43 -0.5 1,649,361 - cysD DVU1566 0.44 -0.4 1,649,471 + cysD DVU1566 0.60 -0.6 1,649,471 + cysD DVU1566 0.60 +0.2 1,649,615 + cysD DVU1566 0.82 -0.8 1,649,632 + cysD DVU1566 0.84 -0.7 1,649,639 + cysD DVU1566 0.85 +0.2 1,649,639 + cysD DVU1566 0.85 -0.1 1,649,640 + cysD DVU1566 0.85 -1.5 1,649,650 + cysD DVU1566 0.87 +0.6 1,649,745 + -1.3 1,649,745 + -1.5 1,649,747 + -1.0 1,649,753 - -1.0 1,649,769 - -1.2 1,649,883 + DVU1567 0.63 -0.2 1,649,978 - -0.3 1,649,978 - +0.9 1,649,994 + +0.7 1,650,002 - -1.8 1,650,026 - -2.1 1,650,060 + -0.6 1,650,060 + -3.2 1,650,068 - -0.6 1,650,170 - -0.7 1,650,328 + ftn DVU1568 0.40 -2.2 1,650,338 - ftn DVU1568 0.42 -1.2 1,650,338 - ftn DVU1568 0.42 -1.2 1,650,383 - ftn DVU1568 0.51 -1.7 1,650,386 + ftn DVU1568 0.51 -0.9 1,650,408 + ftn DVU1568 0.56 -1.1 1,650,512 - ftn DVU1568 0.76 -0.8 1,650,512 - ftn DVU1568 0.76 -0.9 1,650,517 - ftn DVU1568 0.77 +0.6 1,650,578 - ftn DVU1568 0.89 -1.5 1,650,595 - -1.2 1,650,685 + -0.7 1,650,685 + -2.4 1,650,788 + -0.8 1,650,788 + +4.1 1,650,788 + -0.9 1,650,788 + -0.0 1,650,796 - +1.8 1,650,796 - -0.8 1,650,833 - +0.2 1,650,852 + -1.8 1,650,858 + +1.9 1,650,898 + porA DVU1569 0.11 -3.2 1,650,926 + porA DVU1569 0.13 +1.3 1,650,991 - porA DVU1569 0.16 -0.9 1,651,011 + porA DVU1569 0.18 +0.8 1,651,186 - porA DVU1569 0.28 -3.1 1,651,194 - porA DVU1569 0.28 -0.2 1,651,291 - porA DVU1569 0.34 -0.4 1,651,292 - porA DVU1569 0.34 +2.5 1,651,298 - porA DVU1569 0.34 +1.2 1,651,392 + porA DVU1569 0.39 +0.6 1,651,400 - porA DVU1569 0.40 -1.1 1,651,400 - porA DVU1569 0.40 -1.5 1,651,490 - porA DVU1569 0.45 -1.1 1,651,621 + porA DVU1569 0.53 -0.8
Or see this region's nucleotide sequence