Experiment: MoLS4 with L-Cysteine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiC and DVU1412 are separated by 321 nucleotides DVU1412 and DVU1413 are separated by 113 nucleotides
DVU1411: thiC - thiamine biosynthesis protein ThiC (TIGR), at 1,480,019 to 1,481,308
thiC
DVU1412: DVU1412 - D-isomer specific 2-hydroxyacid dehydrogenase family protein (TIGR), at 1,481,630 to 1,482,610
DVU1412
DVU1413: DVU1413 - conserved hypothetical protein (TIGR), at 1,482,724 to 1,484,016
DVU1413
Position (kb)
1481
1482
1483 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1480.698 kb on + strand, within thiC at 1480.756 kb on + strand, within thiC at 1480.763 kb on - strand, within thiC at 1480.850 kb on + strand, within thiC at 1480.867 kb on + strand, within thiC at 1481.012 kb on - strand, within thiC at 1481.143 kb on - strand, within thiC at 1481.156 kb on + strand, within thiC at 1481.164 kb on - strand, within thiC at 1481.164 kb on - strand, within thiC at 1481.164 kb on - strand, within thiC at 1481.202 kb on + strand at 1481.247 kb on - strand at 1481.270 kb on + strand at 1481.310 kb on - strand at 1481.310 kb on - strand at 1481.471 kb on + strand at 1481.545 kb on + strand at 1481.677 kb on - strand at 1481.716 kb on + strand at 1481.722 kb on - strand at 1481.745 kb on + strand, within DVU1412 at 1481.907 kb on + strand, within DVU1412 at 1481.917 kb on - strand, within DVU1412 at 1481.965 kb on - strand, within DVU1412 at 1481.990 kb on + strand, within DVU1412 at 1482.012 kb on + strand, within DVU1412 at 1482.014 kb on + strand, within DVU1412 at 1482.202 kb on - strand, within DVU1412 at 1482.256 kb on + strand, within DVU1412 at 1482.347 kb on + strand, within DVU1412 at 1482.347 kb on + strand, within DVU1412 at 1482.380 kb on + strand, within DVU1412 at 1482.380 kb on + strand, within DVU1412 at 1482.476 kb on + strand, within DVU1412 at 1482.510 kb on - strand, within DVU1412 at 1482.595 kb on + strand at 1482.715 kb on + strand at 1482.718 kb on - strand at 1482.795 kb on - strand at 1482.848 kb on + strand at 1482.902 kb on - strand, within DVU1413 at 1482.981 kb on - strand, within DVU1413 at 1483.067 kb on - strand, within DVU1413 at 1483.101 kb on - strand, within DVU1413 at 1483.101 kb on - strand, within DVU1413 at 1483.118 kb on - strand, within DVU1413 at 1483.175 kb on + strand, within DVU1413 at 1483.180 kb on + strand, within DVU1413 at 1483.180 kb on + strand, within DVU1413 at 1483.214 kb on + strand, within DVU1413 at 1483.372 kb on + strand, within DVU1413 at 1483.375 kb on - strand, within DVU1413 at 1483.411 kb on + strand, within DVU1413 at 1483.411 kb on + strand, within DVU1413 at 1483.499 kb on + strand, within DVU1413 at 1483.547 kb on + strand, within DVU1413
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM remove 1,480,698 + thiC DVU1411 0.53 -0.5 1,480,756 + thiC DVU1411 0.57 +0.0 1,480,763 - thiC DVU1411 0.58 -1.8 1,480,850 + thiC DVU1411 0.64 -2.6 1,480,867 + thiC DVU1411 0.66 -0.6 1,481,012 - thiC DVU1411 0.77 +0.9 1,481,143 - thiC DVU1411 0.87 +0.5 1,481,156 + thiC DVU1411 0.88 +0.3 1,481,164 - thiC DVU1411 0.89 -0.4 1,481,164 - thiC DVU1411 0.89 -2.2 1,481,164 - thiC DVU1411 0.89 -1.7 1,481,202 + +0.7 1,481,247 - -1.4 1,481,270 + -1.5 1,481,310 - -0.6 1,481,310 - -1.0 1,481,471 + +0.7 1,481,545 + +0.8 1,481,677 - -1.7 1,481,716 + -0.7 1,481,722 - -0.4 1,481,745 + DVU1412 0.12 -0.7 1,481,907 + DVU1412 0.28 -0.6 1,481,917 - DVU1412 0.29 -0.7 1,481,965 - DVU1412 0.34 +0.4 1,481,990 + DVU1412 0.37 -1.2 1,482,012 + DVU1412 0.39 +2.1 1,482,014 + DVU1412 0.39 +1.5 1,482,202 - DVU1412 0.58 -0.8 1,482,256 + DVU1412 0.64 -0.4 1,482,347 + DVU1412 0.73 -0.4 1,482,347 + DVU1412 0.73 +0.0 1,482,380 + DVU1412 0.76 -0.1 1,482,380 + DVU1412 0.76 +1.9 1,482,476 + DVU1412 0.86 +0.6 1,482,510 - DVU1412 0.90 +0.4 1,482,595 + -0.4 1,482,715 + +0.1 1,482,718 - -0.7 1,482,795 - -0.1 1,482,848 + +0.5 1,482,902 - DVU1413 0.14 +0.3 1,482,981 - DVU1413 0.20 +0.2 1,483,067 - DVU1413 0.27 -1.3 1,483,101 - DVU1413 0.29 -0.3 1,483,101 - DVU1413 0.29 -1.9 1,483,118 - DVU1413 0.30 -0.3 1,483,175 + DVU1413 0.35 -0.5 1,483,180 + DVU1413 0.35 -0.2 1,483,180 + DVU1413 0.35 -0.4 1,483,214 + DVU1413 0.38 -0.2 1,483,372 + DVU1413 0.50 -0.9 1,483,375 - DVU1413 0.50 -1.7 1,483,411 + DVU1413 0.53 +3.5 1,483,411 + DVU1413 0.53 -2.4 1,483,499 + DVU1413 0.60 -0.5 1,483,547 + DVU1413 0.64 +1.0
Or see this region's nucleotide sequence