Experiment: exometabolites; 5% oxygen; 5 mM nitrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02285 and RR42_RS02290 overlap by 4 nucleotides RR42_RS02290 and RR42_RS02295 overlap by 4 nucleotides
RR42_RS02285: RR42_RS02285 - mannose-1-phosphate guanyltransferase, at 511,869 to 513,047
_RS02285
RR42_RS02290: RR42_RS02290 - multidrug ABC transporter ATP-binding protein, at 513,044 to 514,057
_RS02290
RR42_RS02295: RR42_RS02295 - secretion protein HlyD, at 514,054 to 515,007
_RS02295
Position (kb)
513
514
515 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 512.082 kb on + strand, within RR42_RS02285 at 512.105 kb on - strand, within RR42_RS02285 at 512.105 kb on - strand, within RR42_RS02285 at 512.105 kb on - strand, within RR42_RS02285 at 512.206 kb on + strand, within RR42_RS02285 at 512.206 kb on + strand, within RR42_RS02285 at 512.206 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.299 kb on + strand, within RR42_RS02285 at 512.300 kb on - strand, within RR42_RS02285 at 512.458 kb on + strand, within RR42_RS02285 at 512.459 kb on - strand, within RR42_RS02285 at 512.459 kb on - strand, within RR42_RS02285 at 512.773 kb on + strand, within RR42_RS02285 at 512.774 kb on - strand, within RR42_RS02285 at 512.774 kb on - strand, within RR42_RS02285 at 512.774 kb on - strand, within RR42_RS02285 at 513.232 kb on + strand, within RR42_RS02290 at 513.394 kb on - strand, within RR42_RS02290 at 513.401 kb on - strand, within RR42_RS02290 at 513.402 kb on + strand, within RR42_RS02290 at 513.403 kb on - strand, within RR42_RS02290 at 513.439 kb on - strand, within RR42_RS02290 at 513.439 kb on - strand, within RR42_RS02290 at 513.699 kb on + strand, within RR42_RS02290 at 513.700 kb on - strand, within RR42_RS02290 at 513.771 kb on + strand, within RR42_RS02290 at 513.771 kb on + strand, within RR42_RS02290 at 513.772 kb on - strand, within RR42_RS02290 at 513.973 kb on - strand at 514.163 kb on + strand, within RR42_RS02295 at 514.164 kb on - strand, within RR42_RS02295 at 514.164 kb on - strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.202 kb on + strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.203 kb on - strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.298 kb on + strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.299 kb on - strand, within RR42_RS02295 at 514.388 kb on + strand, within RR42_RS02295 at 514.389 kb on - strand, within RR42_RS02295 at 514.389 kb on - strand, within RR42_RS02295 at 514.429 kb on + strand, within RR42_RS02295 at 514.622 kb on + strand, within RR42_RS02295 at 514.919 kb on + strand at 514.919 kb on + strand at 514.919 kb on + strand at 514.919 kb on + strand at 514.920 kb on - strand at 514.960 kb on + strand at 514.960 kb on + strand at 514.961 kb on - strand at 514.961 kb on - strand at 514.961 kb on - strand at 514.961 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction exometabolites; 5% oxygen; 5 mM nitrate remove 512,082 + RR42_RS02285 0.18 -1.7 512,105 - RR42_RS02285 0.20 -0.1 512,105 - RR42_RS02285 0.20 -0.3 512,105 - RR42_RS02285 0.20 +0.3 512,206 + RR42_RS02285 0.29 -0.4 512,206 + RR42_RS02285 0.29 +1.4 512,206 + RR42_RS02285 0.29 -0.0 512,299 + RR42_RS02285 0.36 -0.6 512,299 + RR42_RS02285 0.36 -0.3 512,299 + RR42_RS02285 0.36 -0.3 512,299 + RR42_RS02285 0.36 +0.1 512,299 + RR42_RS02285 0.36 -0.4 512,300 - RR42_RS02285 0.37 -0.5 512,458 + RR42_RS02285 0.50 -1.0 512,459 - RR42_RS02285 0.50 +1.1 512,459 - RR42_RS02285 0.50 +0.9 512,773 + RR42_RS02285 0.77 -0.4 512,774 - RR42_RS02285 0.77 -0.8 512,774 - RR42_RS02285 0.77 -0.1 512,774 - RR42_RS02285 0.77 -1.9 513,232 + RR42_RS02290 0.19 -0.4 513,394 - RR42_RS02290 0.35 +0.2 513,401 - RR42_RS02290 0.35 -0.2 513,402 + RR42_RS02290 0.35 -1.9 513,403 - RR42_RS02290 0.35 -0.1 513,439 - RR42_RS02290 0.39 +0.2 513,439 - RR42_RS02290 0.39 +0.8 513,699 + RR42_RS02290 0.65 +0.2 513,700 - RR42_RS02290 0.65 -0.5 513,771 + RR42_RS02290 0.72 -0.4 513,771 + RR42_RS02290 0.72 +0.6 513,772 - RR42_RS02290 0.72 -0.5 513,973 - -0.2 514,163 + RR42_RS02295 0.11 +0.3 514,164 - RR42_RS02295 0.12 -0.4 514,164 - RR42_RS02295 0.12 +0.6 514,202 + RR42_RS02295 0.16 +0.8 514,202 + RR42_RS02295 0.16 -0.8 514,202 + RR42_RS02295 0.16 +1.7 514,202 + RR42_RS02295 0.16 -0.1 514,202 + RR42_RS02295 0.16 -1.2 514,203 - RR42_RS02295 0.16 -2.6 514,203 - RR42_RS02295 0.16 +0.4 514,203 - RR42_RS02295 0.16 -0.7 514,203 - RR42_RS02295 0.16 +0.8 514,203 - RR42_RS02295 0.16 +0.1 514,298 + RR42_RS02295 0.26 +0.5 514,298 + RR42_RS02295 0.26 +1.8 514,298 + RR42_RS02295 0.26 -0.8 514,299 - RR42_RS02295 0.26 +1.3 514,299 - RR42_RS02295 0.26 +0.4 514,299 - RR42_RS02295 0.26 +0.4 514,388 + RR42_RS02295 0.35 -0.2 514,389 - RR42_RS02295 0.35 +1.0 514,389 - RR42_RS02295 0.35 +0.6 514,429 + RR42_RS02295 0.39 -0.4 514,622 + RR42_RS02295 0.60 -0.2 514,919 + +0.9 514,919 + +1.8 514,919 + +0.3 514,919 + +0.9 514,920 - +1.1 514,960 + -0.1 514,960 + -0.2 514,961 - +0.1 514,961 - -0.2 514,961 - +0.7 514,961 - +0.6
Or see this region's nucleotide sequence