Experiment: 15:15 LD cycle bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt comE and tRNA-Ser are separated by 37 nucleotides tRNA-Ser and Synpcc7942_2459 are separated by 139 nucleotides Synpcc7942_2459 and Synpcc7942_2460 are separated by 196 nucleotides
Synpcc7942_2458: comE - ComEC/Rec2-related protein, at 2,536,184 to 2,538,358
comE
Synpcc7942_R0016: tRNA-Ser - tRNA-Ser, at 2,538,396 to 2,538,482
tRNA-Ser
Synpcc7942_2459: Synpcc7942_2459 - hypothetical protein, at 2,538,622 to 2,539,710
_2459
Synpcc7942_2460: Synpcc7942_2460 - DNA-cytosine methyltransferase, at 2,539,907 to 2,541,181
_2460
Position (kb)
2538
2539
2540 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2537.624 kb on - strand, within comE at 2537.624 kb on - strand, within comE at 2537.629 kb on + strand, within comE at 2537.638 kb on + strand, within comE at 2537.640 kb on + strand, within comE at 2537.643 kb on + strand, within comE at 2537.648 kb on - strand, within comE at 2537.648 kb on - strand, within comE at 2537.648 kb on - strand, within comE at 2537.650 kb on + strand, within comE at 2537.664 kb on - strand, within comE at 2537.669 kb on - strand, within comE at 2537.729 kb on - strand, within comE at 2537.772 kb on + strand, within comE at 2537.780 kb on - strand, within comE at 2537.780 kb on - strand, within comE at 2537.780 kb on - strand, within comE at 2537.780 kb on - strand, within comE at 2537.780 kb on - strand, within comE at 2537.797 kb on - strand, within comE at 2537.807 kb on - strand, within comE at 2537.813 kb on + strand, within comE at 2537.817 kb on - strand, within comE at 2537.821 kb on - strand, within comE at 2537.854 kb on + strand, within comE at 2537.860 kb on + strand, within comE at 2537.860 kb on + strand, within comE at 2537.868 kb on - strand, within comE at 2537.873 kb on - strand, within comE at 2537.873 kb on - strand, within comE at 2537.873 kb on - strand, within comE at 2537.878 kb on - strand, within comE at 2537.878 kb on - strand, within comE at 2537.894 kb on + strand, within comE at 2537.902 kb on - strand, within comE at 2537.917 kb on + strand, within comE at 2537.925 kb on - strand, within comE at 2537.927 kb on + strand, within comE at 2537.927 kb on + strand, within comE at 2537.928 kb on - strand, within comE at 2537.971 kb on + strand, within comE at 2537.971 kb on - strand, within comE at 2538.030 kb on + strand, within comE at 2538.038 kb on - strand, within comE at 2538.083 kb on - strand, within comE at 2538.083 kb on - strand, within comE at 2538.096 kb on - strand, within comE at 2538.105 kb on - strand, within comE at 2538.121 kb on + strand, within comE at 2538.172 kb on + strand at 2538.186 kb on - strand at 2538.188 kb on + strand at 2538.216 kb on + strand at 2538.216 kb on + strand at 2538.216 kb on + strand at 2538.221 kb on + strand at 2538.254 kb on + strand at 2538.254 kb on + strand at 2538.280 kb on + strand at 2538.280 kb on + strand at 2538.288 kb on - strand at 2538.288 kb on - strand at 2538.510 kb on + strand at 2538.518 kb on - strand at 2538.518 kb on - strand at 2538.561 kb on - strand at 2538.572 kb on - strand at 2539.070 kb on + strand, within Synpcc7942_2459 at 2539.098 kb on + strand, within Synpcc7942_2459 at 2539.128 kb on - strand, within Synpcc7942_2459 at 2539.351 kb on + strand, within Synpcc7942_2459 at 2539.425 kb on + strand, within Synpcc7942_2459 at 2539.425 kb on + strand, within Synpcc7942_2459 at 2539.433 kb on - strand, within Synpcc7942_2459 at 2539.435 kb on + strand, within Synpcc7942_2459 at 2539.443 kb on - strand, within Synpcc7942_2459 at 2539.443 kb on - strand, within Synpcc7942_2459 at 2539.548 kb on - strand, within Synpcc7942_2459 at 2539.793 kb on - strand at 2539.813 kb on + strand at 2539.833 kb on - strand at 2539.971 kb on - strand at 2539.971 kb on - strand at 2539.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 15:15 LD cycle bioreactor remove 2,537,624 - comE Synpcc7942_2458 0.66 -0.1 2,537,624 - comE Synpcc7942_2458 0.66 -0.4 2,537,629 + comE Synpcc7942_2458 0.66 +1.6 2,537,638 + comE Synpcc7942_2458 0.67 -0.7 2,537,640 + comE Synpcc7942_2458 0.67 -0.3 2,537,643 + comE Synpcc7942_2458 0.67 -1.0 2,537,648 - comE Synpcc7942_2458 0.67 -1.6 2,537,648 - comE Synpcc7942_2458 0.67 +2.9 2,537,648 - comE Synpcc7942_2458 0.67 -0.6 2,537,650 + comE Synpcc7942_2458 0.67 -0.6 2,537,664 - comE Synpcc7942_2458 0.68 -1.0 2,537,669 - comE Synpcc7942_2458 0.68 +0.2 2,537,729 - comE Synpcc7942_2458 0.71 +1.6 2,537,772 + comE Synpcc7942_2458 0.73 +1.6 2,537,780 - comE Synpcc7942_2458 0.73 +0.6 2,537,780 - comE Synpcc7942_2458 0.73 -0.7 2,537,780 - comE Synpcc7942_2458 0.73 -0.7 2,537,780 - comE Synpcc7942_2458 0.73 +0.6 2,537,780 - comE Synpcc7942_2458 0.73 -0.4 2,537,797 - comE Synpcc7942_2458 0.74 -0.8 2,537,807 - comE Synpcc7942_2458 0.75 +0.1 2,537,813 + comE Synpcc7942_2458 0.75 +0.6 2,537,817 - comE Synpcc7942_2458 0.75 -1.2 2,537,821 - comE Synpcc7942_2458 0.75 -1.4 2,537,854 + comE Synpcc7942_2458 0.77 -1.1 2,537,860 + comE Synpcc7942_2458 0.77 -1.0 2,537,860 + comE Synpcc7942_2458 0.77 +0.6 2,537,868 - comE Synpcc7942_2458 0.77 -1.3 2,537,873 - comE Synpcc7942_2458 0.78 +0.0 2,537,873 - comE Synpcc7942_2458 0.78 +1.0 2,537,873 - comE Synpcc7942_2458 0.78 -0.5 2,537,878 - comE Synpcc7942_2458 0.78 +2.6 2,537,878 - comE Synpcc7942_2458 0.78 +0.3 2,537,894 + comE Synpcc7942_2458 0.79 +0.3 2,537,902 - comE Synpcc7942_2458 0.79 -0.7 2,537,917 + comE Synpcc7942_2458 0.80 -2.3 2,537,925 - comE Synpcc7942_2458 0.80 -0.9 2,537,927 + comE Synpcc7942_2458 0.80 +0.5 2,537,927 + comE Synpcc7942_2458 0.80 +0.6 2,537,928 - comE Synpcc7942_2458 0.80 +0.0 2,537,971 + comE Synpcc7942_2458 0.82 +1.6 2,537,971 - comE Synpcc7942_2458 0.82 -0.9 2,538,030 + comE Synpcc7942_2458 0.85 +2.6 2,538,038 - comE Synpcc7942_2458 0.85 +0.1 2,538,083 - comE Synpcc7942_2458 0.87 -0.6 2,538,083 - comE Synpcc7942_2458 0.87 -0.7 2,538,096 - comE Synpcc7942_2458 0.88 +1.6 2,538,105 - comE Synpcc7942_2458 0.88 -1.9 2,538,121 + comE Synpcc7942_2458 0.89 -0.3 2,538,172 + +0.8 2,538,186 - -1.2 2,538,188 + +0.6 2,538,216 + -0.7 2,538,216 + +0.6 2,538,216 + -1.0 2,538,221 + +0.3 2,538,254 + +0.4 2,538,254 + +0.6 2,538,280 + -1.1 2,538,280 + -0.4 2,538,288 - -0.7 2,538,288 - -0.4 2,538,510 + +0.1 2,538,518 - -0.8 2,538,518 - -0.6 2,538,561 - -0.5 2,538,572 - -0.4 2,539,070 + Synpcc7942_2459 0.41 -0.6 2,539,098 + Synpcc7942_2459 0.44 -1.6 2,539,128 - Synpcc7942_2459 0.46 -0.7 2,539,351 + Synpcc7942_2459 0.67 +0.0 2,539,425 + Synpcc7942_2459 0.74 +0.2 2,539,425 + Synpcc7942_2459 0.74 -0.7 2,539,433 - Synpcc7942_2459 0.74 +0.9 2,539,435 + Synpcc7942_2459 0.75 +0.0 2,539,443 - Synpcc7942_2459 0.75 -0.9 2,539,443 - Synpcc7942_2459 0.75 -0.5 2,539,548 - Synpcc7942_2459 0.85 -1.1 2,539,793 - +0.3 2,539,813 + -0.1 2,539,833 - -0.7 2,539,971 - +0.0 2,539,971 - +1.6 2,539,971 - -0.4
Or see this region's nucleotide sequence