Experiment: 15:15 LD cycle bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnA and Synpcc7942_2157 are separated by 73 nucleotides Synpcc7942_2157 and apcF are separated by 35 nucleotides apcF and Synpcc7942_2159 are separated by 89 nucleotides Synpcc7942_2159 and nhtA are separated by 61 nucleotides
Synpcc7942_2156: glnA - L-glutamine synthetase, at 2,239,305 to 2,240,726
glnA
Synpcc7942_2157: Synpcc7942_2157 - hypothetical protein, at 2,240,800 to 2,241,084
_2157
Synpcc7942_2158: apcF - allophycocyanin, beta subunit, at 2,241,120 to 2,241,629
apcF
Synpcc7942_2159: Synpcc7942_2159 - hypothetical protein, at 2,241,719 to 2,242,651
_2159
Synpcc7942_2160: nhtA - alanine-glyoxylate aminotransferase, at 2,242,713 to 2,243,867
nhtA
Position (kb)
2241
2242
2243 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2240.725 kb on - strand at 2240.730 kb on - strand at 2240.756 kb on + strand at 2240.761 kb on + strand at 2240.782 kb on - strand at 2240.806 kb on - strand at 2240.806 kb on - strand at 2240.820 kb on + strand at 2240.882 kb on - strand, within Synpcc7942_2157 at 2240.887 kb on - strand, within Synpcc7942_2157 at 2240.935 kb on + strand, within Synpcc7942_2157 at 2240.945 kb on + strand, within Synpcc7942_2157 at 2240.950 kb on + strand, within Synpcc7942_2157 at 2241.028 kb on + strand, within Synpcc7942_2157 at 2241.028 kb on + strand, within Synpcc7942_2157 at 2241.036 kb on - strand, within Synpcc7942_2157 at 2241.109 kb on - strand at 2241.112 kb on - strand at 2241.151 kb on + strand at 2241.216 kb on - strand, within apcF at 2241.227 kb on - strand, within apcF at 2241.276 kb on - strand, within apcF at 2241.304 kb on - strand, within apcF at 2241.353 kb on - strand at 2241.381 kb on - strand, within apcF at 2241.399 kb on + strand, within apcF at 2241.399 kb on + strand, within apcF at 2241.399 kb on + strand, within apcF at 2241.399 kb on + strand, within apcF at 2241.407 kb on - strand, within apcF at 2241.411 kb on - strand at 2241.414 kb on + strand, within apcF at 2241.422 kb on - strand, within apcF at 2241.465 kb on + strand, within apcF at 2241.473 kb on + strand, within apcF at 2241.493 kb on + strand, within apcF at 2241.493 kb on + strand, within apcF at 2241.493 kb on + strand, within apcF at 2241.511 kb on + strand, within apcF at 2241.516 kb on - strand, within apcF at 2241.551 kb on + strand, within apcF at 2241.559 kb on - strand, within apcF at 2241.563 kb on - strand, within apcF at 2241.568 kb on - strand, within apcF at 2241.596 kb on + strand at 2241.615 kb on - strand at 2241.615 kb on - strand at 2241.623 kb on + strand at 2241.623 kb on - strand at 2241.731 kb on - strand at 2241.749 kb on + strand at 2241.750 kb on + strand at 2241.813 kb on + strand, within Synpcc7942_2159 at 2241.844 kb on - strand, within Synpcc7942_2159 at 2241.886 kb on + strand, within Synpcc7942_2159 at 2241.894 kb on - strand, within Synpcc7942_2159 at 2241.896 kb on + strand, within Synpcc7942_2159 at 2241.929 kb on + strand, within Synpcc7942_2159 at 2241.929 kb on + strand, within Synpcc7942_2159 at 2241.964 kb on - strand, within Synpcc7942_2159 at 2241.972 kb on - strand, within Synpcc7942_2159 at 2241.979 kb on + strand, within Synpcc7942_2159 at 2242.009 kb on - strand, within Synpcc7942_2159 at 2242.042 kb on + strand, within Synpcc7942_2159 at 2242.042 kb on + strand, within Synpcc7942_2159 at 2242.042 kb on + strand, within Synpcc7942_2159 at 2242.050 kb on - strand, within Synpcc7942_2159 at 2242.067 kb on + strand, within Synpcc7942_2159 at 2242.074 kb on + strand, within Synpcc7942_2159 at 2242.075 kb on - strand, within Synpcc7942_2159 at 2242.094 kb on + strand, within Synpcc7942_2159 at 2242.094 kb on + strand, within Synpcc7942_2159 at 2242.099 kb on + strand, within Synpcc7942_2159 at 2242.100 kb on + strand, within Synpcc7942_2159 at 2242.108 kb on - strand, within Synpcc7942_2159 at 2242.182 kb on + strand, within Synpcc7942_2159 at 2242.185 kb on - strand, within Synpcc7942_2159 at 2242.197 kb on - strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.199 kb on + strand, within Synpcc7942_2159 at 2242.202 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.207 kb on - strand, within Synpcc7942_2159 at 2242.268 kb on + strand, within Synpcc7942_2159 at 2242.270 kb on + strand, within Synpcc7942_2159 at 2242.276 kb on - strand, within Synpcc7942_2159 at 2242.338 kb on - strand, within Synpcc7942_2159 at 2242.345 kb on - strand, within Synpcc7942_2159 at 2242.352 kb on + strand, within Synpcc7942_2159 at 2242.392 kb on - strand, within Synpcc7942_2159 at 2242.444 kb on + strand, within Synpcc7942_2159 at 2242.471 kb on + strand, within Synpcc7942_2159 at 2242.479 kb on - strand, within Synpcc7942_2159 at 2242.506 kb on - strand, within Synpcc7942_2159 at 2242.511 kb on + strand, within Synpcc7942_2159 at 2242.519 kb on - strand, within Synpcc7942_2159 at 2242.528 kb on + strand, within Synpcc7942_2159 at 2242.536 kb on - strand, within Synpcc7942_2159 at 2242.537 kb on - strand, within Synpcc7942_2159 at 2242.545 kb on + strand, within Synpcc7942_2159 at 2242.545 kb on + strand, within Synpcc7942_2159 at 2242.553 kb on - strand, within Synpcc7942_2159 at 2242.567 kb on + strand at 2242.585 kb on - strand at 2242.590 kb on + strand at 2242.598 kb on - strand at 2242.598 kb on - strand at 2242.598 kb on - strand at 2242.600 kb on + strand at 2242.600 kb on + strand at 2242.600 kb on + strand at 2242.608 kb on - strand at 2242.608 kb on - strand at 2242.622 kb on + strand at 2242.630 kb on - strand at 2242.641 kb on - strand at 2242.643 kb on + strand at 2242.683 kb on - strand at 2242.703 kb on + strand at 2242.746 kb on + strand at 2242.798 kb on - strand at 2242.808 kb on - strand at 2242.826 kb on + strand at 2242.901 kb on - strand, within nhtA at 2242.971 kb on - strand at 2242.993 kb on - strand, within nhtA at 2243.097 kb on - strand, within nhtA at 2243.120 kb on - strand, within nhtA at 2243.153 kb on - strand, within nhtA at 2243.202 kb on + strand, within nhtA at 2243.210 kb on - strand, within nhtA at 2243.462 kb on + strand, within nhtA
Per-strain Table
Position Strand Gene LocusTag Fraction 15:15 LD cycle bioreactor remove 2,240,725 - +4.6 2,240,730 - +1.6 2,240,756 + +1.2 2,240,761 + +4.0 2,240,782 - +1.0 2,240,806 - +0.0 2,240,806 - +2.6 2,240,820 + +3.5 2,240,882 - Synpcc7942_2157 0.29 +0.8 2,240,887 - Synpcc7942_2157 0.31 +1.8 2,240,935 + Synpcc7942_2157 0.47 +1.3 2,240,945 + Synpcc7942_2157 0.51 -0.5 2,240,950 + Synpcc7942_2157 0.53 +0.1 2,241,028 + Synpcc7942_2157 0.80 +1.6 2,241,028 + Synpcc7942_2157 0.80 +0.6 2,241,036 - Synpcc7942_2157 0.83 -0.6 2,241,109 - -1.1 2,241,112 - -0.7 2,241,151 + -1.2 2,241,216 - apcF Synpcc7942_2158 0.19 +0.2 2,241,227 - apcF Synpcc7942_2158 0.21 +3.2 2,241,276 - apcF Synpcc7942_2158 0.31 -0.0 2,241,304 - apcF Synpcc7942_2158 0.36 -0.1 2,241,353 - -0.4 2,241,381 - apcF Synpcc7942_2158 0.51 -0.7 2,241,399 + apcF Synpcc7942_2158 0.55 -0.1 2,241,399 + apcF Synpcc7942_2158 0.55 -2.3 2,241,399 + apcF Synpcc7942_2158 0.55 -1.1 2,241,399 + apcF Synpcc7942_2158 0.55 -0.7 2,241,407 - apcF Synpcc7942_2158 0.56 -0.3 2,241,411 - +0.5 2,241,414 + apcF Synpcc7942_2158 0.58 -0.1 2,241,422 - apcF Synpcc7942_2158 0.59 -0.6 2,241,465 + apcF Synpcc7942_2158 0.68 -0.6 2,241,473 + apcF Synpcc7942_2158 0.69 -0.6 2,241,493 + apcF Synpcc7942_2158 0.73 -1.0 2,241,493 + apcF Synpcc7942_2158 0.73 -1.0 2,241,493 + apcF Synpcc7942_2158 0.73 -0.6 2,241,511 + apcF Synpcc7942_2158 0.77 -0.6 2,241,516 - apcF Synpcc7942_2158 0.78 +0.3 2,241,551 + apcF Synpcc7942_2158 0.85 +0.6 2,241,559 - apcF Synpcc7942_2158 0.86 -1.1 2,241,563 - apcF Synpcc7942_2158 0.87 +0.2 2,241,568 - apcF Synpcc7942_2158 0.88 -0.8 2,241,596 + +0.2 2,241,615 - -1.4 2,241,615 - +0.5 2,241,623 + -0.9 2,241,623 - -0.2 2,241,731 - +0.0 2,241,749 + -1.6 2,241,750 + -0.7 2,241,813 + Synpcc7942_2159 0.10 -0.7 2,241,844 - Synpcc7942_2159 0.13 +1.0 2,241,886 + Synpcc7942_2159 0.18 +0.0 2,241,894 - Synpcc7942_2159 0.19 -0.3 2,241,896 + Synpcc7942_2159 0.19 +0.0 2,241,929 + Synpcc7942_2159 0.23 -0.1 2,241,929 + Synpcc7942_2159 0.23 -0.1 2,241,964 - Synpcc7942_2159 0.26 +0.2 2,241,972 - Synpcc7942_2159 0.27 -1.2 2,241,979 + Synpcc7942_2159 0.28 -0.6 2,242,009 - Synpcc7942_2159 0.31 -0.9 2,242,042 + Synpcc7942_2159 0.35 -1.3 2,242,042 + Synpcc7942_2159 0.35 -1.2 2,242,042 + Synpcc7942_2159 0.35 -1.0 2,242,050 - Synpcc7942_2159 0.35 -0.4 2,242,067 + Synpcc7942_2159 0.37 -0.4 2,242,074 + Synpcc7942_2159 0.38 -0.0 2,242,075 - Synpcc7942_2159 0.38 -0.2 2,242,094 + Synpcc7942_2159 0.40 -0.1 2,242,094 + Synpcc7942_2159 0.40 +1.0 2,242,099 + Synpcc7942_2159 0.41 -0.2 2,242,100 + Synpcc7942_2159 0.41 -1.4 2,242,108 - Synpcc7942_2159 0.42 +1.6 2,242,182 + Synpcc7942_2159 0.50 -0.6 2,242,185 - Synpcc7942_2159 0.50 -0.8 2,242,197 - Synpcc7942_2159 0.51 -0.5 2,242,199 + Synpcc7942_2159 0.51 -0.7 2,242,199 + Synpcc7942_2159 0.51 -0.8 2,242,199 + Synpcc7942_2159 0.51 -0.8 2,242,199 + Synpcc7942_2159 0.51 -1.6 2,242,199 + Synpcc7942_2159 0.51 -0.4 2,242,199 + Synpcc7942_2159 0.51 -0.9 2,242,199 + Synpcc7942_2159 0.51 -1.2 2,242,199 + Synpcc7942_2159 0.51 +1.0 2,242,199 + Synpcc7942_2159 0.51 +0.0 2,242,199 + Synpcc7942_2159 0.51 -1.2 2,242,199 + Synpcc7942_2159 0.51 -0.4 2,242,199 + Synpcc7942_2159 0.51 -0.7 2,242,199 + Synpcc7942_2159 0.51 +0.2 2,242,199 + Synpcc7942_2159 0.51 +1.0 2,242,199 + Synpcc7942_2159 0.51 -2.4 2,242,202 - Synpcc7942_2159 0.52 +0.2 2,242,207 - Synpcc7942_2159 0.52 -0.4 2,242,207 - Synpcc7942_2159 0.52 -0.9 2,242,207 - Synpcc7942_2159 0.52 -0.9 2,242,207 - Synpcc7942_2159 0.52 -0.7 2,242,207 - Synpcc7942_2159 0.52 +2.0 2,242,207 - Synpcc7942_2159 0.52 -0.8 2,242,207 - Synpcc7942_2159 0.52 +0.0 2,242,207 - Synpcc7942_2159 0.52 +0.0 2,242,207 - Synpcc7942_2159 0.52 -0.9 2,242,207 - Synpcc7942_2159 0.52 -1.4 2,242,207 - Synpcc7942_2159 0.52 -0.5 2,242,207 - Synpcc7942_2159 0.52 -0.2 2,242,207 - Synpcc7942_2159 0.52 -0.2 2,242,207 - Synpcc7942_2159 0.52 +0.4 2,242,207 - Synpcc7942_2159 0.52 -0.2 2,242,268 + Synpcc7942_2159 0.59 +0.9 2,242,270 + Synpcc7942_2159 0.59 +0.0 2,242,276 - Synpcc7942_2159 0.60 +0.4 2,242,338 - Synpcc7942_2159 0.66 +1.0 2,242,345 - Synpcc7942_2159 0.67 +0.2 2,242,352 + Synpcc7942_2159 0.68 +0.4 2,242,392 - Synpcc7942_2159 0.72 +1.0 2,242,444 + Synpcc7942_2159 0.78 +1.6 2,242,471 + Synpcc7942_2159 0.81 -1.0 2,242,479 - Synpcc7942_2159 0.81 -0.9 2,242,506 - Synpcc7942_2159 0.84 -0.8 2,242,511 + Synpcc7942_2159 0.85 -0.1 2,242,519 - Synpcc7942_2159 0.86 +1.9 2,242,528 + Synpcc7942_2159 0.87 -0.2 2,242,536 - Synpcc7942_2159 0.88 +0.3 2,242,537 - Synpcc7942_2159 0.88 +1.6 2,242,545 + Synpcc7942_2159 0.89 -1.7 2,242,545 + Synpcc7942_2159 0.89 -0.9 2,242,553 - Synpcc7942_2159 0.89 +0.0 2,242,567 + -0.8 2,242,585 - -1.4 2,242,590 + -0.5 2,242,598 - +0.8 2,242,598 - -0.7 2,242,598 - -1.2 2,242,600 + -0.5 2,242,600 + +1.4 2,242,600 + +0.3 2,242,608 - -0.7 2,242,608 - +0.4 2,242,622 + +0.8 2,242,630 - -0.1 2,242,641 - -1.0 2,242,643 + +1.5 2,242,683 - +2.5 2,242,703 + -0.5 2,242,746 + +2.3 2,242,798 - +1.8 2,242,808 - -0.7 2,242,826 + +1.3 2,242,901 - nhtA Synpcc7942_2160 0.16 +1.6 2,242,971 - +0.5 2,242,993 - nhtA Synpcc7942_2160 0.24 +2.8 2,243,097 - nhtA Synpcc7942_2160 0.33 +1.7 2,243,120 - nhtA Synpcc7942_2160 0.35 +2.4 2,243,153 - nhtA Synpcc7942_2160 0.38 +2.1 2,243,202 + nhtA Synpcc7942_2160 0.42 +2.0 2,243,210 - nhtA Synpcc7942_2160 0.43 +2.8 2,243,462 + nhtA Synpcc7942_2160 0.65 +1.4
Or see this region's nucleotide sequence