Experiment: 15:15 LD cycle bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt modA and Synpcc7942_1532 overlap by 4 nucleotides Synpcc7942_1532 and sdhB overlap by 23 nucleotides sdhB and Synpcc7942_1534 are separated by 121 nucleotides
Synpcc7942_1531: modA - molybdenum ABC transporter, periplasmic molybdate-binding protein, at 1,590,799 to 1,591,614
modA
Synpcc7942_1532: Synpcc7942_1532 - Molybdate ABC transporter, permease protein, at 1,591,611 to 1,592,276
_1532
Synpcc7942_1533: sdhB - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 1,592,254 to 1,593,093
sdhB
Synpcc7942_1534: Synpcc7942_1534 - hypothetical protein, at 1,593,215 to 1,595,422
_1534
Position (kb)
1592
1593
1594 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1591.284 kb on + strand, within modA at 1591.373 kb on - strand, within modA at 1591.462 kb on + strand, within modA at 1591.559 kb on - strand at 1591.628 kb on - strand at 1591.628 kb on - strand at 1592.051 kb on + strand, within Synpcc7942_1532 at 1592.230 kb on - strand at 1592.249 kb on + strand at 1592.249 kb on + strand at 1592.271 kb on + strand at 1592.314 kb on - strand at 1592.327 kb on + strand at 1592.327 kb on + strand at 1592.335 kb on - strand at 1592.342 kb on - strand, within sdhB at 1592.390 kb on + strand, within sdhB at 1592.396 kb on - strand, within sdhB at 1592.398 kb on - strand, within sdhB at 1592.400 kb on + strand, within sdhB at 1592.400 kb on + strand, within sdhB at 1592.408 kb on + strand, within sdhB at 1592.408 kb on - strand, within sdhB at 1592.419 kb on + strand, within sdhB at 1592.445 kb on + strand, within sdhB at 1592.451 kb on - strand, within sdhB at 1592.460 kb on - strand, within sdhB at 1592.485 kb on - strand, within sdhB at 1592.489 kb on + strand, within sdhB at 1592.497 kb on - strand, within sdhB at 1592.551 kb on - strand, within sdhB at 1592.560 kb on + strand, within sdhB at 1592.596 kb on + strand, within sdhB at 1592.602 kb on + strand, within sdhB at 1592.647 kb on - strand, within sdhB at 1592.664 kb on - strand, within sdhB at 1592.664 kb on - strand, within sdhB at 1592.674 kb on + strand, within sdhB at 1592.674 kb on + strand at 1592.681 kb on - strand, within sdhB at 1592.682 kb on - strand, within sdhB at 1592.721 kb on + strand, within sdhB at 1592.728 kb on + strand, within sdhB at 1592.733 kb on - strand, within sdhB at 1592.733 kb on - strand, within sdhB at 1592.795 kb on - strand, within sdhB at 1592.848 kb on - strand, within sdhB at 1592.950 kb on - strand, within sdhB at 1592.961 kb on + strand, within sdhB at 1592.982 kb on - strand, within sdhB at 1592.982 kb on - strand, within sdhB at 1593.037 kb on + strand at 1593.042 kb on + strand at 1593.055 kb on - strand at 1593.096 kb on - strand at 1593.123 kb on + strand at 1593.123 kb on + strand at 1593.140 kb on + strand at 1593.171 kb on + strand at 1593.171 kb on + strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.189 kb on - strand at 1593.189 kb on - strand at 1593.218 kb on + strand at 1593.459 kb on + strand, within Synpcc7942_1534 at 1593.514 kb on + strand, within Synpcc7942_1534 at 1593.576 kb on + strand, within Synpcc7942_1534 at 1593.628 kb on + strand, within Synpcc7942_1534 at 1593.655 kb on + strand, within Synpcc7942_1534 at 1593.742 kb on + strand, within Synpcc7942_1534 at 1593.742 kb on + strand, within Synpcc7942_1534 at 1593.750 kb on - strand, within Synpcc7942_1534 at 1593.809 kb on - strand, within Synpcc7942_1534 at 1593.824 kb on + strand, within Synpcc7942_1534 at 1593.861 kb on + strand, within Synpcc7942_1534 at 1594.088 kb on + strand, within Synpcc7942_1534 at 1594.088 kb on + strand, within Synpcc7942_1534
Per-strain Table
Position Strand Gene LocusTag Fraction 15:15 LD cycle bioreactor remove 1,591,284 + modA Synpcc7942_1531 0.59 +2.8 1,591,373 - modA Synpcc7942_1531 0.70 +0.6 1,591,462 + modA Synpcc7942_1531 0.81 -0.4 1,591,559 - +1.3 1,591,628 - +0.8 1,591,628 - -1.4 1,592,051 + Synpcc7942_1532 0.66 +0.1 1,592,230 - +0.1 1,592,249 + -1.6 1,592,249 + +0.1 1,592,271 + -1.5 1,592,314 - +0.3 1,592,327 + +1.0 1,592,327 + +0.5 1,592,335 - -0.5 1,592,342 - sdhB Synpcc7942_1533 0.10 +0.4 1,592,390 + sdhB Synpcc7942_1533 0.16 -0.8 1,592,396 - sdhB Synpcc7942_1533 0.17 +0.2 1,592,398 - sdhB Synpcc7942_1533 0.17 +0.4 1,592,400 + sdhB Synpcc7942_1533 0.17 +0.4 1,592,400 + sdhB Synpcc7942_1533 0.17 +1.1 1,592,408 + sdhB Synpcc7942_1533 0.18 -0.7 1,592,408 - sdhB Synpcc7942_1533 0.18 +1.2 1,592,419 + sdhB Synpcc7942_1533 0.20 +1.7 1,592,445 + sdhB Synpcc7942_1533 0.23 +0.9 1,592,451 - sdhB Synpcc7942_1533 0.23 +0.6 1,592,460 - sdhB Synpcc7942_1533 0.25 +1.3 1,592,485 - sdhB Synpcc7942_1533 0.28 +0.7 1,592,489 + sdhB Synpcc7942_1533 0.28 +1.4 1,592,497 - sdhB Synpcc7942_1533 0.29 +1.6 1,592,551 - sdhB Synpcc7942_1533 0.35 -0.1 1,592,560 + sdhB Synpcc7942_1533 0.36 +0.1 1,592,596 + sdhB Synpcc7942_1533 0.41 +0.2 1,592,602 + sdhB Synpcc7942_1533 0.41 +0.9 1,592,647 - sdhB Synpcc7942_1533 0.47 +1.3 1,592,664 - sdhB Synpcc7942_1533 0.49 +0.5 1,592,664 - sdhB Synpcc7942_1533 0.49 +0.6 1,592,674 + sdhB Synpcc7942_1533 0.50 +1.1 1,592,674 + +0.1 1,592,681 - sdhB Synpcc7942_1533 0.51 +0.1 1,592,682 - sdhB Synpcc7942_1533 0.51 +1.0 1,592,721 + sdhB Synpcc7942_1533 0.56 +1.6 1,592,728 + sdhB Synpcc7942_1533 0.56 +0.2 1,592,733 - sdhB Synpcc7942_1533 0.57 +2.5 1,592,733 - sdhB Synpcc7942_1533 0.57 +1.5 1,592,795 - sdhB Synpcc7942_1533 0.64 +1.6 1,592,848 - sdhB Synpcc7942_1533 0.71 +1.4 1,592,950 - sdhB Synpcc7942_1533 0.83 +0.6 1,592,961 + sdhB Synpcc7942_1533 0.84 +1.8 1,592,982 - sdhB Synpcc7942_1533 0.87 +1.7 1,592,982 - sdhB Synpcc7942_1533 0.87 +0.9 1,593,037 + +1.6 1,593,042 + +2.1 1,593,055 - +0.4 1,593,096 - +1.2 1,593,123 + +0.6 1,593,123 + -0.1 1,593,140 + +0.1 1,593,171 + -0.9 1,593,171 + +0.1 1,593,179 - +1.6 1,593,179 - -1.5 1,593,179 - -1.4 1,593,179 - -1.5 1,593,179 - +0.0 1,593,179 - -1.0 1,593,189 - -1.1 1,593,189 - +1.6 1,593,218 + -0.3 1,593,459 + Synpcc7942_1534 0.11 -0.5 1,593,514 + Synpcc7942_1534 0.14 -0.7 1,593,576 + Synpcc7942_1534 0.16 -0.2 1,593,628 + Synpcc7942_1534 0.19 +0.1 1,593,655 + Synpcc7942_1534 0.20 -1.3 1,593,742 + Synpcc7942_1534 0.24 -2.3 1,593,742 + Synpcc7942_1534 0.24 -0.2 1,593,750 - Synpcc7942_1534 0.24 -0.3 1,593,809 - Synpcc7942_1534 0.27 -0.1 1,593,824 + Synpcc7942_1534 0.28 +1.0 1,593,861 + Synpcc7942_1534 0.29 +1.4 1,594,088 + Synpcc7942_1534 0.40 -0.2 1,594,088 + Synpcc7942_1534 0.40 +1.6
Or see this region's nucleotide sequence