Experiment: 15:15 LD cycle bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mazG and Synpcc7942_1494 are separated by 28 nucleotides Synpcc7942_1494 and Synpcc7942_1495 are separated by 27 nucleotides Synpcc7942_1495 and argB overlap by 4 nucleotides argB and Synpcc7942_1497 overlap by 8 nucleotides
Synpcc7942_1493: mazG - nucleoside triphosphate pyrophosphohydrolase, at 1,544,514 to 1,545,353
mazG
Synpcc7942_1494: Synpcc7942_1494 - hypothetical protein, at 1,545,382 to 1,546,095
_1494
Synpcc7942_1495: Synpcc7942_1495 - hypothetical protein, at 1,546,123 to 1,546,650
_1495
Synpcc7942_1496: argB - acetylglutamate kinase, at 1,546,647 to 1,547,552
argB
Synpcc7942_1497: Synpcc7942_1497 - hypothetical protein, at 1,547,545 to 1,548,105
_1497
Position (kb)
1546
1547 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1545.131 kb on + strand, within mazG at 1545.139 kb on - strand, within mazG at 1545.139 kb on - strand, within mazG at 1545.143 kb on + strand, within mazG at 1545.144 kb on - strand, within mazG at 1545.167 kb on + strand, within mazG at 1545.175 kb on - strand, within mazG at 1545.203 kb on - strand, within mazG at 1545.207 kb on + strand, within mazG at 1545.207 kb on + strand, within mazG at 1545.209 kb on - strand, within mazG at 1545.210 kb on + strand, within mazG at 1545.215 kb on - strand, within mazG at 1545.223 kb on - strand, within mazG at 1545.270 kb on - strand at 1545.272 kb on + strand at 1545.282 kb on + strand at 1545.290 kb on - strand at 1545.331 kb on + strand at 1545.336 kb on + strand at 1545.348 kb on + strand at 1545.348 kb on + strand at 1545.348 kb on + strand at 1545.367 kb on - strand at 1545.380 kb on + strand at 1545.380 kb on + strand at 1545.395 kb on - strand at 1545.405 kb on - strand at 1545.565 kb on - strand, within Synpcc7942_1494 at 1546.101 kb on + strand at 1546.109 kb on - strand at 1546.109 kb on - strand at 1546.111 kb on - strand at 1546.111 kb on - strand at 1546.114 kb on + strand at 1546.119 kb on + strand at 1546.120 kb on + strand at 1546.127 kb on - strand at 1546.160 kb on + strand at 1546.168 kb on - strand at 1546.173 kb on + strand at 1546.173 kb on + strand at 1546.210 kb on - strand, within Synpcc7942_1495 at 1546.237 kb on - strand, within Synpcc7942_1495 at 1546.251 kb on - strand, within Synpcc7942_1495 at 1546.260 kb on - strand, within Synpcc7942_1495 at 1546.312 kb on - strand, within Synpcc7942_1495 at 1546.323 kb on + strand, within Synpcc7942_1495 at 1546.326 kb on - strand, within Synpcc7942_1495 at 1546.331 kb on - strand, within Synpcc7942_1495 at 1546.338 kb on - strand, within Synpcc7942_1495 at 1546.378 kb on + strand, within Synpcc7942_1495 at 1546.379 kb on + strand, within Synpcc7942_1495 at 1546.379 kb on + strand, within Synpcc7942_1495 at 1546.379 kb on + strand, within Synpcc7942_1495 at 1546.382 kb on - strand, within Synpcc7942_1495 at 1546.387 kb on - strand, within Synpcc7942_1495 at 1546.387 kb on - strand, within Synpcc7942_1495 at 1546.400 kb on - strand, within Synpcc7942_1495 at 1546.404 kb on + strand, within Synpcc7942_1495 at 1546.427 kb on - strand, within Synpcc7942_1495 at 1546.434 kb on + strand, within Synpcc7942_1495 at 1546.448 kb on + strand, within Synpcc7942_1495 at 1546.457 kb on + strand, within Synpcc7942_1495 at 1546.457 kb on + strand, within Synpcc7942_1495 at 1546.458 kb on + strand, within Synpcc7942_1495 at 1546.465 kb on - strand, within Synpcc7942_1495 at 1546.506 kb on + strand, within Synpcc7942_1495 at 1546.514 kb on - strand, within Synpcc7942_1495 at 1546.514 kb on - strand, within Synpcc7942_1495 at 1546.528 kb on - strand, within Synpcc7942_1495 at 1546.545 kb on - strand, within Synpcc7942_1495 at 1546.566 kb on - strand, within Synpcc7942_1495 at 1546.569 kb on + strand, within Synpcc7942_1495 at 1546.623 kb on - strand at 1546.623 kb on - strand at 1546.623 kb on - strand at 1546.623 kb on - strand at 1546.654 kb on - strand at 1547.600 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 15:15 LD cycle bioreactor remove 1,545,131 + mazG Synpcc7942_1493 0.73 -0.8 1,545,139 - mazG Synpcc7942_1493 0.74 -0.4 1,545,139 - mazG Synpcc7942_1493 0.74 -0.4 1,545,143 + mazG Synpcc7942_1493 0.75 -0.4 1,545,144 - mazG Synpcc7942_1493 0.75 +0.6 1,545,167 + mazG Synpcc7942_1493 0.78 +2.2 1,545,175 - mazG Synpcc7942_1493 0.79 -2.0 1,545,203 - mazG Synpcc7942_1493 0.82 -0.5 1,545,207 + mazG Synpcc7942_1493 0.82 +0.3 1,545,207 + mazG Synpcc7942_1493 0.82 -1.8 1,545,209 - mazG Synpcc7942_1493 0.83 -1.0 1,545,210 + mazG Synpcc7942_1493 0.83 -0.7 1,545,215 - mazG Synpcc7942_1493 0.83 +0.6 1,545,223 - mazG Synpcc7942_1493 0.84 -1.0 1,545,270 - +0.2 1,545,272 + +0.7 1,545,282 + +0.9 1,545,290 - +0.5 1,545,331 + +0.6 1,545,336 + -0.4 1,545,348 + +0.0 1,545,348 + -0.1 1,545,348 + -1.6 1,545,367 - -0.4 1,545,380 + +0.8 1,545,380 + -0.7 1,545,395 - +2.6 1,545,405 - -0.8 1,545,565 - Synpcc7942_1494 0.26 -2.8 1,546,101 + -0.8 1,546,109 - -1.4 1,546,109 - +0.3 1,546,111 - -0.5 1,546,111 - -0.3 1,546,114 + -1.5 1,546,119 + -1.5 1,546,120 + +1.6 1,546,127 - +0.6 1,546,160 + -0.3 1,546,168 - +3.4 1,546,173 + -0.3 1,546,173 + -0.6 1,546,210 - Synpcc7942_1495 0.16 -1.5 1,546,237 - Synpcc7942_1495 0.22 -2.1 1,546,251 - Synpcc7942_1495 0.24 -1.9 1,546,260 - Synpcc7942_1495 0.26 +1.6 1,546,312 - Synpcc7942_1495 0.36 -1.3 1,546,323 + Synpcc7942_1495 0.38 -1.3 1,546,326 - Synpcc7942_1495 0.38 -1.3 1,546,331 - Synpcc7942_1495 0.39 -1.2 1,546,338 - Synpcc7942_1495 0.41 -0.9 1,546,378 + Synpcc7942_1495 0.48 -0.7 1,546,379 + Synpcc7942_1495 0.48 -0.1 1,546,379 + Synpcc7942_1495 0.48 -1.1 1,546,379 + Synpcc7942_1495 0.48 +1.0 1,546,382 - Synpcc7942_1495 0.49 +0.3 1,546,387 - Synpcc7942_1495 0.50 -1.3 1,546,387 - Synpcc7942_1495 0.50 +1.0 1,546,400 - Synpcc7942_1495 0.52 -0.1 1,546,404 + Synpcc7942_1495 0.53 +0.0 1,546,427 - Synpcc7942_1495 0.58 -0.4 1,546,434 + Synpcc7942_1495 0.59 +0.6 1,546,448 + Synpcc7942_1495 0.62 -0.7 1,546,457 + Synpcc7942_1495 0.63 -0.5 1,546,457 + Synpcc7942_1495 0.63 -1.5 1,546,458 + Synpcc7942_1495 0.63 -0.1 1,546,465 - Synpcc7942_1495 0.65 +0.5 1,546,506 + Synpcc7942_1495 0.73 +0.6 1,546,514 - Synpcc7942_1495 0.74 -0.4 1,546,514 - Synpcc7942_1495 0.74 -1.0 1,546,528 - Synpcc7942_1495 0.77 -0.4 1,546,545 - Synpcc7942_1495 0.80 -0.6 1,546,566 - Synpcc7942_1495 0.84 -0.1 1,546,569 + Synpcc7942_1495 0.84 -0.8 1,546,623 - -0.6 1,546,623 - -1.0 1,546,623 - +0.0 1,546,623 - +0.6 1,546,654 - -1.2 1,547,600 + +2.4
Or see this region's nucleotide sequence