Experiment: 15:15 LD cycle bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutS and gltA are separated by 3 nucleotides gltA and sixA are separated by 134 nucleotides sixA and Synpcc7942_0614 are separated by 3 nucleotides
Synpcc7942_0611: mutS - recombination and DNA strand exchange inhibitor protein, at 599,815 to 602,205
mutS
Synpcc7942_0612: gltA - methylcitrate synthase, at 602,209 to 603,369
gltA
Synpcc7942_0613: sixA - phosphohistidine phosphatase, SixA, at 603,504 to 603,992
sixA
Synpcc7942_0614: Synpcc7942_0614 - hypothetical protein, at 603,996 to 605,675
_0614
Position (kb) 602
603
604 Strain fitness (log2 ratio) -2
-1
0
1 at 601.272 kb on + strand, within mutS at 601.272 kb on + strand, within mutS at 601.272 kb on + strand, within mutS at 601.280 kb on - strand, within mutS at 601.280 kb on - strand, within mutS at 601.295 kb on + strand at 601.298 kb on - strand, within mutS at 601.298 kb on - strand, within mutS at 601.333 kb on - strand, within mutS at 601.355 kb on - strand, within mutS at 601.426 kb on - strand, within mutS at 601.453 kb on - strand at 601.453 kb on - strand, within mutS at 601.476 kb on + strand, within mutS at 601.479 kb on - strand, within mutS at 601.515 kb on + strand, within mutS at 601.515 kb on + strand, within mutS at 601.515 kb on + strand, within mutS at 601.523 kb on - strand, within mutS at 601.533 kb on - strand, within mutS at 601.571 kb on - strand, within mutS at 601.617 kb on + strand, within mutS at 601.630 kb on + strand, within mutS at 601.638 kb on - strand, within mutS at 601.648 kb on - strand, within mutS at 601.686 kb on + strand, within mutS at 601.717 kb on + strand, within mutS at 601.779 kb on + strand, within mutS at 601.793 kb on - strand, within mutS at 601.823 kb on + strand, within mutS at 601.823 kb on + strand, within mutS at 601.831 kb on - strand, within mutS at 601.844 kb on + strand, within mutS at 601.885 kb on - strand, within mutS at 601.923 kb on + strand, within mutS at 601.931 kb on - strand, within mutS at 601.981 kb on + strand at 601.982 kb on + strand at 601.990 kb on - strand at 601.990 kb on - strand at 601.990 kb on - strand at 602.008 kb on - strand at 602.038 kb on - strand at 602.112 kb on + strand at 602.154 kb on + strand at 602.192 kb on + strand at 602.199 kb on + strand at 602.200 kb on + strand at 602.200 kb on + strand at 602.202 kb on - strand at 602.208 kb on - strand at 603.455 kb on - strand at 603.490 kb on + strand at 603.510 kb on - strand at 603.510 kb on - strand at 603.510 kb on - strand at 603.510 kb on - strand at 603.529 kb on + strand at 603.569 kb on - strand, within sixA at 603.583 kb on - strand, within sixA at 603.603 kb on - strand, within sixA at 603.603 kb on - strand, within sixA at 603.615 kb on + strand, within sixA at 603.615 kb on + strand, within sixA at 603.615 kb on + strand, within sixA at 603.618 kb on - strand, within sixA at 603.623 kb on - strand, within sixA at 603.623 kb on - strand, within sixA at 603.641 kb on + strand, within sixA at 603.661 kb on - strand, within sixA at 603.661 kb on - strand, within sixA at 603.661 kb on - strand, within sixA at 603.687 kb on + strand, within sixA at 603.687 kb on + strand, within sixA at 603.732 kb on + strand, within sixA at 603.780 kb on + strand, within sixA at 603.825 kb on + strand, within sixA at 603.828 kb on - strand, within sixA at 603.838 kb on + strand, within sixA at 603.845 kb on - strand, within sixA at 603.850 kb on + strand, within sixA at 603.905 kb on + strand, within sixA at 603.913 kb on + strand, within sixA at 603.994 kb on + strand at 604.037 kb on - strand at 604.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 15:15 LD cycle bioreactor remove 601,272 + mutS Synpcc7942_0611 0.61 -0.9 601,272 + mutS Synpcc7942_0611 0.61 +1.7 601,272 + mutS Synpcc7942_0611 0.61 -0.2 601,280 - mutS Synpcc7942_0611 0.61 -0.3 601,280 - mutS Synpcc7942_0611 0.61 -0.4 601,295 + -0.7 601,298 - mutS Synpcc7942_0611 0.62 -0.2 601,298 - mutS Synpcc7942_0611 0.62 -0.1 601,333 - mutS Synpcc7942_0611 0.63 -1.0 601,355 - mutS Synpcc7942_0611 0.64 +0.3 601,426 - mutS Synpcc7942_0611 0.67 -0.4 601,453 - -0.1 601,453 - mutS Synpcc7942_0611 0.69 +1.1 601,476 + mutS Synpcc7942_0611 0.69 -0.3 601,479 - mutS Synpcc7942_0611 0.70 -0.3 601,515 + mutS Synpcc7942_0611 0.71 +0.1 601,515 + mutS Synpcc7942_0611 0.71 -0.1 601,515 + mutS Synpcc7942_0611 0.71 +0.4 601,523 - mutS Synpcc7942_0611 0.71 -0.7 601,533 - mutS Synpcc7942_0611 0.72 -0.3 601,571 - mutS Synpcc7942_0611 0.73 +0.4 601,617 + mutS Synpcc7942_0611 0.75 +0.5 601,630 + mutS Synpcc7942_0611 0.76 +0.3 601,638 - mutS Synpcc7942_0611 0.76 -1.6 601,648 - mutS Synpcc7942_0611 0.77 -0.2 601,686 + mutS Synpcc7942_0611 0.78 -0.5 601,717 + mutS Synpcc7942_0611 0.80 -0.7 601,779 + mutS Synpcc7942_0611 0.82 +0.7 601,793 - mutS Synpcc7942_0611 0.83 +1.1 601,823 + mutS Synpcc7942_0611 0.84 +0.1 601,823 + mutS Synpcc7942_0611 0.84 +0.1 601,831 - mutS Synpcc7942_0611 0.84 -0.5 601,844 + mutS Synpcc7942_0611 0.85 +0.1 601,885 - mutS Synpcc7942_0611 0.87 +0.7 601,923 + mutS Synpcc7942_0611 0.88 +1.2 601,931 - mutS Synpcc7942_0611 0.88 +0.7 601,981 + -1.0 601,982 + -0.9 601,990 - -0.7 601,990 - -0.5 601,990 - +1.5 602,008 - +0.7 602,038 - -0.3 602,112 + +0.3 602,154 + +0.9 602,192 + -0.5 602,199 + -0.1 602,200 + -0.2 602,200 + -0.3 602,202 - +0.1 602,208 - -1.7 603,455 - -0.3 603,490 + -1.2 603,510 - +1.7 603,510 - -0.7 603,510 - -0.0 603,510 - +0.1 603,529 + +1.7 603,569 - sixA Synpcc7942_0613 0.13 -0.7 603,583 - sixA Synpcc7942_0613 0.16 -0.6 603,603 - sixA Synpcc7942_0613 0.20 +0.3 603,603 - sixA Synpcc7942_0613 0.20 -1.0 603,615 + sixA Synpcc7942_0613 0.23 -0.7 603,615 + sixA Synpcc7942_0613 0.23 -0.5 603,615 + sixA Synpcc7942_0613 0.23 -0.9 603,618 - sixA Synpcc7942_0613 0.23 -1.1 603,623 - sixA Synpcc7942_0613 0.24 +1.7 603,623 - sixA Synpcc7942_0613 0.24 -0.3 603,641 + sixA Synpcc7942_0613 0.28 +0.1 603,661 - sixA Synpcc7942_0613 0.32 -1.2 603,661 - sixA Synpcc7942_0613 0.32 -0.7 603,661 - sixA Synpcc7942_0613 0.32 -0.4 603,687 + sixA Synpcc7942_0613 0.37 -0.5 603,687 + sixA Synpcc7942_0613 0.37 -0.7 603,732 + sixA Synpcc7942_0613 0.47 +0.2 603,780 + sixA Synpcc7942_0613 0.56 -2.0 603,825 + sixA Synpcc7942_0613 0.66 +0.7 603,828 - sixA Synpcc7942_0613 0.66 +0.8 603,838 + sixA Synpcc7942_0613 0.68 -0.3 603,845 - sixA Synpcc7942_0613 0.70 -0.3 603,850 + sixA Synpcc7942_0613 0.71 +0.1 603,905 + sixA Synpcc7942_0613 0.82 +0.2 603,913 + sixA Synpcc7942_0613 0.84 -0.4 603,994 + -0.5 604,037 - -0.3 604,052 - -0.3
Or see this region's nucleotide sequence