Experiment: 15:15 LD cycle bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ama and Synpcc7942_0257 overlap by 4 nucleotides Synpcc7942_0257 and Synpcc7942_0258 are separated by 48 nucleotides Synpcc7942_0258 and Synpcc7942_0259 are separated by 139 nucleotides Synpcc7942_0259 and clpC are separated by 154 nucleotides
Synpcc7942_0256: ama - Peptidase M20D, amidohydrolase, at 252,873 to 254,099
ama
Synpcc7942_0257: Synpcc7942_0257 - Protein of unknown function DUF92, transmembrane, at 254,096 to 254,845
_0257
Synpcc7942_0258: Synpcc7942_0258 - hypothetical protein, at 254,894 to 255,334
_0258
Synpcc7942_0259: Synpcc7942_0259 - hypothetical protein, at 255,474 to 255,806
_0259
Synpcc7942_0260: clpC - ATPase, at 255,961 to 258,435
clpC
Position (kb) 254
255
256 Strain fitness (log2 ratio) -2
-1
0
1
2 at 253.925 kb on + strand, within ama at 253.931 kb on - strand, within ama at 253.933 kb on - strand, within ama at 253.939 kb on - strand, within ama at 253.939 kb on - strand, within ama at 253.950 kb on - strand, within ama at 254.052 kb on - strand at 254.829 kb on + strand at 254.839 kb on + strand at 254.839 kb on + strand at 254.846 kb on + strand at 254.847 kb on - strand at 254.849 kb on - strand at 254.909 kb on + strand at 254.917 kb on - strand at 254.917 kb on - strand at 254.977 kb on - strand, within Synpcc7942_0258 at 255.044 kb on + strand, within Synpcc7942_0258 at 255.044 kb on + strand, within Synpcc7942_0258 at 255.044 kb on + strand, within Synpcc7942_0258 at 255.052 kb on - strand, within Synpcc7942_0258 at 255.052 kb on - strand, within Synpcc7942_0258 at 255.052 kb on - strand, within Synpcc7942_0258 at 255.052 kb on - strand, within Synpcc7942_0258 at 255.054 kb on - strand, within Synpcc7942_0258 at 255.067 kb on - strand, within Synpcc7942_0258 at 255.076 kb on - strand, within Synpcc7942_0258 at 255.078 kb on + strand, within Synpcc7942_0258 at 255.101 kb on - strand, within Synpcc7942_0258 at 255.102 kb on - strand, within Synpcc7942_0258 at 255.103 kb on + strand, within Synpcc7942_0258 at 255.121 kb on - strand, within Synpcc7942_0258 at 255.194 kb on + strand, within Synpcc7942_0258 at 255.212 kb on - strand, within Synpcc7942_0258 at 255.293 kb on + strand at 255.335 kb on - strand at 255.398 kb on + strand at 255.445 kb on - strand at 255.477 kb on + strand at 255.485 kb on - strand at 255.596 kb on - strand, within Synpcc7942_0259 at 255.596 kb on - strand, within Synpcc7942_0259 at 255.600 kb on - strand, within Synpcc7942_0259 at 255.615 kb on + strand, within Synpcc7942_0259 at 255.639 kb on + strand at 255.693 kb on + strand, within Synpcc7942_0259 at 255.728 kb on + strand, within Synpcc7942_0259 at 255.762 kb on + strand, within Synpcc7942_0259 at 255.762 kb on - strand, within Synpcc7942_0259 at 255.856 kb on - strand at 255.856 kb on - strand at 255.915 kb on - strand at 255.927 kb on - strand at 255.933 kb on - strand at 255.943 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 15:15 LD cycle bioreactor remove 253,925 + ama Synpcc7942_0256 0.86 +1.6 253,931 - ama Synpcc7942_0256 0.86 +0.6 253,933 - ama Synpcc7942_0256 0.86 -0.7 253,939 - ama Synpcc7942_0256 0.87 -0.2 253,939 - ama Synpcc7942_0256 0.87 -0.7 253,950 - ama Synpcc7942_0256 0.88 +1.6 254,052 - -0.7 254,829 + -1.2 254,839 + -1.1 254,839 + -0.5 254,846 + -0.3 254,847 - -0.7 254,849 - -2.0 254,909 + -0.8 254,917 - +2.6 254,917 - -0.1 254,977 - Synpcc7942_0258 0.19 -1.4 255,044 + Synpcc7942_0258 0.34 -0.5 255,044 + Synpcc7942_0258 0.34 +0.6 255,044 + Synpcc7942_0258 0.34 -0.3 255,052 - Synpcc7942_0258 0.36 +1.0 255,052 - Synpcc7942_0258 0.36 -0.9 255,052 - Synpcc7942_0258 0.36 -1.8 255,052 - Synpcc7942_0258 0.36 -1.3 255,054 - Synpcc7942_0258 0.36 -1.7 255,067 - Synpcc7942_0258 0.39 -0.9 255,076 - Synpcc7942_0258 0.41 -1.5 255,078 + Synpcc7942_0258 0.42 +0.0 255,101 - Synpcc7942_0258 0.47 -1.3 255,102 - Synpcc7942_0258 0.47 -2.2 255,103 + Synpcc7942_0258 0.47 +0.0 255,121 - Synpcc7942_0258 0.51 -0.4 255,194 + Synpcc7942_0258 0.68 -0.1 255,212 - Synpcc7942_0258 0.72 +0.2 255,293 + +1.4 255,335 - -1.4 255,398 + -1.2 255,445 - +0.2 255,477 + -2.1 255,485 - +0.3 255,596 - Synpcc7942_0259 0.37 +2.6 255,596 - Synpcc7942_0259 0.37 +0.0 255,600 - Synpcc7942_0259 0.38 -0.4 255,615 + Synpcc7942_0259 0.42 -0.1 255,639 + -0.4 255,693 + Synpcc7942_0259 0.66 -0.5 255,728 + Synpcc7942_0259 0.76 -1.5 255,762 + Synpcc7942_0259 0.86 +0.3 255,762 - Synpcc7942_0259 0.86 -0.2 255,856 - +0.0 255,856 - -0.4 255,915 - -0.2 255,927 - -0.2 255,933 - -1.2 255,943 + -0.9
Or see this region's nucleotide sequence