Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS04385 and PFLU_RS04390 are separated by 2 nucleotides PFLU_RS04390 and PFLU_RS04395 are separated by 10 nucleotides PFLU_RS04395 and PFLU_RS04400 are separated by 147 nucleotides
PFLU_RS04385: PFLU_RS04385 - RNase adapter RapZ, at 989,555 to 990,412
_RS04385
PFLU_RS04390: PFLU_RS04390 - PTS IIA-like nitrogen regulatory protein PtsN, at 990,415 to 990,879
_RS04390
PFLU_RS04395: PFLU_RS04395 - ribosome-associated translation inhibitor RaiA, at 990,890 to 991,195
_RS04395
PFLU_RS04400: PFLU_RS04400 - RNA polymerase factor sigma-54, at 991,343 to 992,836
_RS04400
Position (kb) 990
991
992 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 989.890 kb on + strand, within PFLU_RS04385 at 989.890 kb on + strand, within PFLU_RS04385 at 989.891 kb on - strand, within PFLU_RS04385 at 989.973 kb on + strand, within PFLU_RS04385 at 989.974 kb on - strand, within PFLU_RS04385 at 989.974 kb on - strand, within PFLU_RS04385 at 989.974 kb on - strand, within PFLU_RS04385 at 989.980 kb on + strand, within PFLU_RS04385 at 989.981 kb on - strand, within PFLU_RS04385 at 989.981 kb on - strand, within PFLU_RS04385 at 990.147 kb on + strand, within PFLU_RS04385 at 990.147 kb on + strand, within PFLU_RS04385 at 990.148 kb on - strand, within PFLU_RS04385 at 990.148 kb on - strand, within PFLU_RS04385 at 990.148 kb on - strand, within PFLU_RS04385 at 990.333 kb on + strand at 990.333 kb on + strand at 990.333 kb on + strand at 990.333 kb on + strand at 990.334 kb on - strand at 990.367 kb on + strand at 990.367 kb on + strand at 990.368 kb on - strand at 990.374 kb on - strand at 990.416 kb on + strand at 990.417 kb on - strand at 990.902 kb on - strand at 990.902 kb on - strand at 990.902 kb on - strand at 990.902 kb on - strand at 990.902 kb on - strand at 990.902 kb on - strand at 990.902 kb on - strand at 990.932 kb on - strand, within PFLU_RS04395 at 990.932 kb on - strand, within PFLU_RS04395 at 990.939 kb on - strand, within PFLU_RS04395 at 990.939 kb on - strand, within PFLU_RS04395 at 990.939 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 990.985 kb on - strand, within PFLU_RS04395 at 991.141 kb on - strand, within PFLU_RS04395 at 991.141 kb on - strand, within PFLU_RS04395 at 991.141 kb on - strand, within PFLU_RS04395 at 991.141 kb on - strand, within PFLU_RS04395 at 991.160 kb on - strand, within PFLU_RS04395 at 991.160 kb on - strand, within PFLU_RS04395 at 991.196 kb on - strand at 991.196 kb on - strand at 991.196 kb on - strand at 991.196 kb on - strand at 991.196 kb on - strand at 991.196 kb on - strand at 991.235 kb on - strand at 991.241 kb on - strand at 991.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs remove 989,890 + PFLU_RS04385 0.39 -1.5 989,890 + PFLU_RS04385 0.39 -0.2 989,891 - PFLU_RS04385 0.39 -1.5 989,973 + PFLU_RS04385 0.49 -1.5 989,974 - PFLU_RS04385 0.49 -1.3 989,974 - PFLU_RS04385 0.49 +2.6 989,974 - PFLU_RS04385 0.49 -0.8 989,980 + PFLU_RS04385 0.50 -1.1 989,981 - PFLU_RS04385 0.50 +2.2 989,981 - PFLU_RS04385 0.50 -1.4 990,147 + PFLU_RS04385 0.69 -0.7 990,147 + PFLU_RS04385 0.69 -0.5 990,148 - PFLU_RS04385 0.69 -1.2 990,148 - PFLU_RS04385 0.69 -2.4 990,148 - PFLU_RS04385 0.69 -0.4 990,333 + +0.5 990,333 + -0.3 990,333 + +0.1 990,333 + -0.6 990,334 - +1.3 990,367 + -1.2 990,367 + +0.7 990,368 - -3.0 990,374 - +0.5 990,416 + +0.7 990,417 - -1.3 990,902 - +0.6 990,902 - +0.2 990,902 - +2.2 990,902 - +1.3 990,902 - +0.6 990,902 - +1.0 990,902 - +0.6 990,932 - PFLU_RS04395 0.14 +0.4 990,932 - PFLU_RS04395 0.14 +1.2 990,939 - PFLU_RS04395 0.16 -0.3 990,939 - PFLU_RS04395 0.16 +0.1 990,939 - PFLU_RS04395 0.16 -1.4 990,985 - PFLU_RS04395 0.31 -0.6 990,985 - PFLU_RS04395 0.31 -0.4 990,985 - PFLU_RS04395 0.31 -0.8 990,985 - PFLU_RS04395 0.31 -0.7 990,985 - PFLU_RS04395 0.31 +1.0 990,985 - PFLU_RS04395 0.31 -1.3 990,985 - PFLU_RS04395 0.31 +1.0 990,985 - PFLU_RS04395 0.31 +1.2 990,985 - PFLU_RS04395 0.31 -1.9 990,985 - PFLU_RS04395 0.31 -0.0 990,985 - PFLU_RS04395 0.31 +0.3 990,985 - PFLU_RS04395 0.31 +0.2 990,985 - PFLU_RS04395 0.31 -1.0 990,985 - PFLU_RS04395 0.31 -1.3 990,985 - PFLU_RS04395 0.31 -1.2 991,141 - PFLU_RS04395 0.82 -0.9 991,141 - PFLU_RS04395 0.82 -1.2 991,141 - PFLU_RS04395 0.82 -1.0 991,141 - PFLU_RS04395 0.82 +0.2 991,160 - PFLU_RS04395 0.88 -0.3 991,160 - PFLU_RS04395 0.88 -0.8 991,196 - -0.1 991,196 - -1.7 991,196 - -2.1 991,196 - -0.8 991,196 - -0.8 991,196 - -0.3 991,235 - +0.7 991,241 - +0.0 991,358 - +1.3
Or see this region's nucleotide sequence