Experiment: MoLS4 with L-Cysteine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2359 and DVU2360 are separated by 103 nucleotides DVU2360 and thiE-2 are separated by 223 nucleotides thiE-2 and thiM overlap by 4 nucleotides
DVU2359: DVU2359 - sigma-54 dependent transcriptional regulator (TIGR), at 2,456,656 to 2,458,311
DVU2359
DVU2360: DVU2360 - oxidoreductase, FAD/NAD-binding family (TIGR), at 2,458,415 to 2,459,221
DVU2360
DVU2362: thiE-2 - thiamine-phosphate pyrophosphorylase (TIGR), at 2,459,445 to 2,460,104
thiE-2
DVU2363: thiM - hydroxyethylthiazole kinase (TIGR), at 2,460,101 to 2,460,904
thiM
Position (kb)
2458
2459
2460 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2457.522 kb on + strand, within DVU2359 at 2457.528 kb on - strand, within DVU2359 at 2457.530 kb on - strand, within DVU2359 at 2457.674 kb on + strand, within DVU2359 at 2457.722 kb on + strand, within DVU2359 at 2457.902 kb on - strand, within DVU2359 at 2457.920 kb on + strand, within DVU2359 at 2457.951 kb on + strand, within DVU2359 at 2457.951 kb on + strand, within DVU2359 at 2458.022 kb on - strand, within DVU2359 at 2458.040 kb on + strand, within DVU2359 at 2458.045 kb on + strand, within DVU2359 at 2458.087 kb on - strand, within DVU2359 at 2458.093 kb on - strand, within DVU2359 at 2458.230 kb on - strand at 2458.369 kb on + strand at 2458.375 kb on - strand at 2458.425 kb on - strand at 2458.528 kb on + strand, within DVU2360 at 2458.558 kb on + strand, within DVU2360 at 2458.627 kb on + strand, within DVU2360 at 2458.634 kb on + strand, within DVU2360 at 2458.637 kb on - strand, within DVU2360 at 2458.677 kb on - strand, within DVU2360 at 2458.711 kb on - strand, within DVU2360 at 2458.801 kb on + strand, within DVU2360 at 2458.839 kb on - strand, within DVU2360 at 2458.895 kb on + strand, within DVU2360 at 2458.908 kb on - strand, within DVU2360 at 2458.930 kb on + strand, within DVU2360 at 2458.965 kb on + strand, within DVU2360 at 2458.973 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.086 kb on - strand, within DVU2360 at 2459.121 kb on - strand, within DVU2360 at 2459.180 kb on + strand at 2459.188 kb on - strand at 2459.204 kb on + strand at 2459.229 kb on + strand at 2459.237 kb on - strand at 2459.237 kb on - strand at 2459.329 kb on + strand at 2459.369 kb on + strand at 2459.370 kb on + strand at 2459.403 kb on - strand at 2459.440 kb on - strand at 2459.485 kb on - strand at 2459.578 kb on + strand, within thiE-2 at 2459.578 kb on + strand, within thiE-2 at 2459.727 kb on + strand, within thiE-2 at 2459.748 kb on - strand, within thiE-2 at 2459.978 kb on + strand, within thiE-2 at 2460.052 kb on - strand at 2460.061 kb on + strand at 2460.103 kb on - strand at 2460.122 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine as nutrient 10mM remove 2,457,522 + DVU2359 0.52 +1.1 2,457,528 - DVU2359 0.53 -0.6 2,457,530 - DVU2359 0.53 -1.2 2,457,674 + DVU2359 0.61 +0.4 2,457,722 + DVU2359 0.64 +2.1 2,457,902 - DVU2359 0.75 -0.7 2,457,920 + DVU2359 0.76 -0.6 2,457,951 + DVU2359 0.78 -1.4 2,457,951 + DVU2359 0.78 +0.9 2,458,022 - DVU2359 0.82 -0.4 2,458,040 + DVU2359 0.84 -1.1 2,458,045 + DVU2359 0.84 -0.4 2,458,087 - DVU2359 0.86 -2.3 2,458,093 - DVU2359 0.87 -1.7 2,458,230 - -0.4 2,458,369 + -0.9 2,458,375 - -0.0 2,458,425 - -4.2 2,458,528 + DVU2360 0.14 -1.1 2,458,558 + DVU2360 0.18 +1.1 2,458,627 + DVU2360 0.26 -1.9 2,458,634 + DVU2360 0.27 +2.3 2,458,637 - DVU2360 0.28 -1.1 2,458,677 - DVU2360 0.32 -1.4 2,458,711 - DVU2360 0.37 -1.9 2,458,801 + DVU2360 0.48 -1.4 2,458,839 - DVU2360 0.53 -2.0 2,458,895 + DVU2360 0.59 +1.8 2,458,908 - DVU2360 0.61 -0.5 2,458,930 + DVU2360 0.64 -0.1 2,458,965 + DVU2360 0.68 -0.4 2,458,973 - DVU2360 0.69 +1.1 2,459,022 - DVU2360 0.75 -1.4 2,459,022 - DVU2360 0.75 -2.1 2,459,022 - DVU2360 0.75 -0.2 2,459,086 - DVU2360 0.83 -2.2 2,459,121 - DVU2360 0.87 +0.7 2,459,180 + +1.6 2,459,188 - +2.1 2,459,204 + -0.6 2,459,229 + -0.8 2,459,237 - -0.2 2,459,237 - +1.0 2,459,329 + +1.4 2,459,369 + -1.4 2,459,370 + +0.8 2,459,403 - +2.6 2,459,440 - -1.5 2,459,485 - +0.5 2,459,578 + thiE-2 DVU2362 0.20 +3.2 2,459,578 + thiE-2 DVU2362 0.20 +4.6 2,459,727 + thiE-2 DVU2362 0.43 -0.5 2,459,748 - thiE-2 DVU2362 0.46 +1.4 2,459,978 + thiE-2 DVU2362 0.81 +1.6 2,460,052 - -2.6 2,460,061 + -0.2 2,460,103 - -0.1 2,460,122 - -1.0
Or see this region's nucleotide sequence