Experiment: 12:12 LD cycle bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspC and Synpcc7942_2546 are separated by 76 nucleotides Synpcc7942_2546 and Synpcc7942_2547 are separated by 48 nucleotides Synpcc7942_2547 and leuD are separated by 97 nucleotides leuD and Synpcc7942_2549 are separated by 72 nucleotides
Synpcc7942_2545: aspC - aspartate aminotransferase, at 2,624,982 to 2,626,160
aspC
Synpcc7942_2546: Synpcc7942_2546 - hypothetical protein, at 2,626,237 to 2,626,956
_2546
Synpcc7942_2547: Synpcc7942_2547 - hypothetical protein, at 2,627,005 to 2,627,325
_2547
Synpcc7942_2548: leuD - isopropylmalate isomerase small subunit, at 2,627,423 to 2,628,031
leuD
Synpcc7942_2549: Synpcc7942_2549 - hypothetical protein, at 2,628,104 to 2,628,358
_2549
Position (kb)
2627
2628 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2626.155 kb on + strand at 2626.169 kb on - strand at 2626.173 kb on - strand at 2626.173 kb on - strand at 2626.183 kb on - strand at 2626.185 kb on + strand at 2626.202 kb on + strand at 2626.207 kb on + strand at 2626.210 kb on - strand at 2626.239 kb on + strand at 2626.239 kb on + strand at 2626.241 kb on - strand at 2626.303 kb on - strand at 2626.305 kb on - strand at 2626.305 kb on - strand at 2626.312 kb on + strand, within Synpcc7942_2546 at 2626.318 kb on - strand, within Synpcc7942_2546 at 2626.341 kb on + strand, within Synpcc7942_2546 at 2626.341 kb on + strand, within Synpcc7942_2546 at 2626.371 kb on + strand, within Synpcc7942_2546 at 2626.375 kb on + strand, within Synpcc7942_2546 at 2626.384 kb on - strand, within Synpcc7942_2546 at 2626.384 kb on - strand, within Synpcc7942_2546 at 2626.386 kb on + strand, within Synpcc7942_2546 at 2626.389 kb on - strand, within Synpcc7942_2546 at 2626.391 kb on - strand, within Synpcc7942_2546 at 2626.415 kb on - strand, within Synpcc7942_2546 at 2626.420 kb on - strand, within Synpcc7942_2546 at 2626.448 kb on - strand, within Synpcc7942_2546 at 2626.494 kb on - strand, within Synpcc7942_2546 at 2626.505 kb on + strand, within Synpcc7942_2546 at 2626.505 kb on + strand, within Synpcc7942_2546 at 2626.513 kb on - strand, within Synpcc7942_2546 at 2626.587 kb on - strand, within Synpcc7942_2546 at 2626.679 kb on + strand, within Synpcc7942_2546 at 2626.687 kb on - strand, within Synpcc7942_2546 at 2626.725 kb on + strand, within Synpcc7942_2546 at 2626.733 kb on - strand, within Synpcc7942_2546 at 2626.754 kb on - strand, within Synpcc7942_2546 at 2626.754 kb on - strand, within Synpcc7942_2546 at 2626.754 kb on - strand, within Synpcc7942_2546 at 2626.761 kb on + strand, within Synpcc7942_2546 at 2626.761 kb on + strand, within Synpcc7942_2546 at 2626.769 kb on - strand, within Synpcc7942_2546 at 2626.769 kb on - strand, within Synpcc7942_2546 at 2626.770 kb on - strand, within Synpcc7942_2546 at 2626.772 kb on + strand, within Synpcc7942_2546 at 2626.780 kb on - strand, within Synpcc7942_2546 at 2626.794 kb on - strand, within Synpcc7942_2546 at 2626.808 kb on - strand, within Synpcc7942_2546 at 2626.863 kb on + strand, within Synpcc7942_2546 at 2626.863 kb on + strand, within Synpcc7942_2546 at 2626.864 kb on + strand, within Synpcc7942_2546 at 2626.864 kb on + strand, within Synpcc7942_2546 at 2626.864 kb on - strand, within Synpcc7942_2546 at 2626.869 kb on + strand, within Synpcc7942_2546 at 2626.869 kb on + strand, within Synpcc7942_2546 at 2626.872 kb on - strand, within Synpcc7942_2546 at 2626.879 kb on + strand, within Synpcc7942_2546 at 2626.879 kb on + strand, within Synpcc7942_2546 at 2626.879 kb on + strand, within Synpcc7942_2546 at 2626.880 kb on + strand, within Synpcc7942_2546 at 2626.888 kb on - strand at 2626.888 kb on - strand at 2626.888 kb on - strand at 2626.888 kb on - strand at 2626.888 kb on - strand at 2626.900 kb on - strand at 2626.937 kb on - strand at 2626.964 kb on + strand at 2626.964 kb on + strand at 2626.964 kb on + strand at 2626.970 kb on - strand at 2626.972 kb on - strand at 2626.993 kb on + strand at 2627.007 kb on - strand at 2627.031 kb on + strand at 2627.045 kb on - strand, within Synpcc7942_2547 at 2627.090 kb on - strand, within Synpcc7942_2547 at 2627.090 kb on - strand, within Synpcc7942_2547 at 2627.120 kb on - strand, within Synpcc7942_2547 at 2627.136 kb on + strand, within Synpcc7942_2547 at 2627.286 kb on - strand, within Synpcc7942_2547 at 2627.287 kb on - strand, within Synpcc7942_2547 at 2627.287 kb on - strand, within Synpcc7942_2547 at 2627.309 kb on - strand at 2627.328 kb on - strand at 2627.343 kb on - strand at 2627.394 kb on + strand at 2627.397 kb on - strand at 2627.397 kb on - strand at 2627.417 kb on + strand at 2628.051 kb on + strand at 2628.051 kb on + strand at 2628.051 kb on + strand at 2628.051 kb on + strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.085 kb on + strand at 2628.090 kb on + strand at 2628.102 kb on + strand at 2628.108 kb on + strand at 2628.187 kb on - strand, within Synpcc7942_2549 at 2628.209 kb on - strand, within Synpcc7942_2549 at 2628.212 kb on + strand, within Synpcc7942_2549 at 2628.222 kb on + strand, within Synpcc7942_2549 at 2628.234 kb on + strand, within Synpcc7942_2549 at 2628.267 kb on + strand, within Synpcc7942_2549 at 2628.284 kb on + strand, within Synpcc7942_2549
Per-strain Table
Position Strand Gene LocusTag Fraction 12:12 LD cycle bioreactor remove 2,626,155 + -0.4 2,626,169 - +0.4 2,626,173 - -1.4 2,626,173 - +1.0 2,626,183 - +0.3 2,626,185 + +0.3 2,626,202 + -0.4 2,626,207 + +2.2 2,626,210 - -0.5 2,626,239 + -0.0 2,626,239 + +0.7 2,626,241 - -0.9 2,626,303 - -1.0 2,626,305 - +0.6 2,626,305 - +1.3 2,626,312 + Synpcc7942_2546 0.10 +1.4 2,626,318 - Synpcc7942_2546 0.11 -0.1 2,626,341 + Synpcc7942_2546 0.14 -0.8 2,626,341 + Synpcc7942_2546 0.14 -0.1 2,626,371 + Synpcc7942_2546 0.19 +1.0 2,626,375 + Synpcc7942_2546 0.19 -0.3 2,626,384 - Synpcc7942_2546 0.20 -0.7 2,626,384 - Synpcc7942_2546 0.20 -0.0 2,626,386 + Synpcc7942_2546 0.21 -0.4 2,626,389 - Synpcc7942_2546 0.21 -0.5 2,626,391 - Synpcc7942_2546 0.21 +1.9 2,626,415 - Synpcc7942_2546 0.25 +0.3 2,626,420 - Synpcc7942_2546 0.25 -0.1 2,626,448 - Synpcc7942_2546 0.29 -0.0 2,626,494 - Synpcc7942_2546 0.36 -0.3 2,626,505 + Synpcc7942_2546 0.37 +0.9 2,626,505 + Synpcc7942_2546 0.37 +1.6 2,626,513 - Synpcc7942_2546 0.38 +0.6 2,626,587 - Synpcc7942_2546 0.49 -0.0 2,626,679 + Synpcc7942_2546 0.61 -0.4 2,626,687 - Synpcc7942_2546 0.62 +0.4 2,626,725 + Synpcc7942_2546 0.68 +0.9 2,626,733 - Synpcc7942_2546 0.69 -0.9 2,626,754 - Synpcc7942_2546 0.72 -0.3 2,626,754 - Synpcc7942_2546 0.72 -0.7 2,626,754 - Synpcc7942_2546 0.72 -1.3 2,626,761 + Synpcc7942_2546 0.73 -1.5 2,626,761 + Synpcc7942_2546 0.73 -0.2 2,626,769 - Synpcc7942_2546 0.74 -0.0 2,626,769 - Synpcc7942_2546 0.74 +1.9 2,626,770 - Synpcc7942_2546 0.74 -1.3 2,626,772 + Synpcc7942_2546 0.74 +0.8 2,626,780 - Synpcc7942_2546 0.75 -0.0 2,626,794 - Synpcc7942_2546 0.77 +0.4 2,626,808 - Synpcc7942_2546 0.79 -0.1 2,626,863 + Synpcc7942_2546 0.87 -0.0 2,626,863 + Synpcc7942_2546 0.87 +0.5 2,626,864 + Synpcc7942_2546 0.87 -1.1 2,626,864 + Synpcc7942_2546 0.87 +0.2 2,626,864 - Synpcc7942_2546 0.87 -0.9 2,626,869 + Synpcc7942_2546 0.88 +0.7 2,626,869 + Synpcc7942_2546 0.88 +0.5 2,626,872 - Synpcc7942_2546 0.88 +0.3 2,626,879 + Synpcc7942_2546 0.89 -0.7 2,626,879 + Synpcc7942_2546 0.89 -0.8 2,626,879 + Synpcc7942_2546 0.89 +0.9 2,626,880 + Synpcc7942_2546 0.89 +0.3 2,626,888 - +0.9 2,626,888 - +1.3 2,626,888 - +1.1 2,626,888 - -0.7 2,626,888 - +0.4 2,626,900 - +1.3 2,626,937 - +1.3 2,626,964 + -0.9 2,626,964 + +1.3 2,626,964 + +0.3 2,626,970 - -0.2 2,626,972 - +0.3 2,626,993 + +1.0 2,627,007 - +0.3 2,627,031 + +0.7 2,627,045 - Synpcc7942_2547 0.12 +0.3 2,627,090 - Synpcc7942_2547 0.26 +1.9 2,627,090 - Synpcc7942_2547 0.26 +0.5 2,627,120 - Synpcc7942_2547 0.36 -0.3 2,627,136 + Synpcc7942_2547 0.41 -0.3 2,627,286 - Synpcc7942_2547 0.88 +0.8 2,627,287 - Synpcc7942_2547 0.88 -0.5 2,627,287 - Synpcc7942_2547 0.88 +0.7 2,627,309 - +0.4 2,627,328 - +1.3 2,627,343 - +0.7 2,627,394 + +0.6 2,627,397 - +1.3 2,627,397 - +0.8 2,627,417 + -0.2 2,628,051 + +1.9 2,628,051 + +2.1 2,628,051 + +1.3 2,628,051 + -0.2 2,628,059 - +1.3 2,628,059 - -0.2 2,628,059 - -0.5 2,628,059 - +0.3 2,628,059 - +0.1 2,628,059 - -1.0 2,628,085 + -0.2 2,628,090 + +1.3 2,628,102 + -0.6 2,628,108 + +0.0 2,628,187 - Synpcc7942_2549 0.33 +1.0 2,628,209 - Synpcc7942_2549 0.41 +0.4 2,628,212 + Synpcc7942_2549 0.42 +0.7 2,628,222 + Synpcc7942_2549 0.46 +0.9 2,628,234 + Synpcc7942_2549 0.51 +0.6 2,628,267 + Synpcc7942_2549 0.64 +2.9 2,628,284 + Synpcc7942_2549 0.71 +1.3
Or see this region's nucleotide sequence