Experiment: H2 McCas with 0.1 mM (NH4)2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06380 and MMP_RS06385 are separated by 5 nucleotides MMP_RS06385 and MMP_RS06390 are separated by 10 nucleotides MMP_RS06390 and MMP_RS06395 are separated by 52 nucleotides MMP_RS06395 and MMP_RS06400 are separated by 203 nucleotides
MMP_RS06380: MMP_RS06380 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,225,667 to 1,226,809
_RS06380
MMP_RS06385: MMP_RS06385 - hypothetical protein, at 1,226,815 to 1,227,174
_RS06385
MMP_RS06390: MMP_RS06390 - quinolinate synthase NadA, at 1,227,185 to 1,228,102
_RS06390
MMP_RS06395: MMP_RS06395 - ThiF family adenylyltransferase, at 1,228,155 to 1,228,769
_RS06395
MMP_RS06400: MMP_RS06400 - 4Fe-4S binding protein, at 1,228,973 to 1,229,419
_RS06400
Position (kb)
1227
1228
1229 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1226.409 kb on - strand at 1226.873 kb on + strand, within MMP_RS06385 at 1226.978 kb on - strand, within MMP_RS06385 at 1226.990 kb on + strand, within MMP_RS06385 at 1227.074 kb on - strand, within MMP_RS06385 at 1227.075 kb on + strand, within MMP_RS06385 at 1227.080 kb on - strand, within MMP_RS06385 at 1227.173 kb on + strand at 1227.710 kb on + strand at 1227.766 kb on - strand at 1227.771 kb on + strand at 1227.779 kb on - strand at 1227.821 kb on + strand at 1228.036 kb on + strand at 1228.094 kb on - strand at 1228.094 kb on - strand at 1228.416 kb on - strand, within MMP_RS06395 at 1228.509 kb on + strand, within MMP_RS06395 at 1228.568 kb on + strand, within MMP_RS06395 at 1228.603 kb on + strand, within MMP_RS06395 at 1228.798 kb on + strand at 1228.837 kb on + strand at 1228.928 kb on - strand at 1228.929 kb on + strand at 1228.929 kb on + strand at 1228.929 kb on + strand at 1228.929 kb on + strand at 1228.951 kb on + strand at 1228.985 kb on + strand at 1228.985 kb on + strand at 1228.985 kb on + strand at 1228.986 kb on - strand at 1228.986 kb on - strand at 1228.987 kb on + strand at 1228.987 kb on + strand at 1228.990 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 0.1 mM (NH4)2S remove 1,226,409 - -2.7 1,226,873 + MMP_RS06385 0.16 +0.8 1,226,978 - MMP_RS06385 0.45 +0.5 1,226,990 + MMP_RS06385 0.49 -0.2 1,227,074 - MMP_RS06385 0.72 -0.1 1,227,075 + MMP_RS06385 0.72 -0.6 1,227,080 - MMP_RS06385 0.74 -0.6 1,227,173 + +0.4 1,227,710 + -2.7 1,227,766 - -1.6 1,227,771 + -2.6 1,227,779 - -1.8 1,227,821 + -2.6 1,228,036 + -4.1 1,228,094 - -2.2 1,228,094 - -2.2 1,228,416 - MMP_RS06395 0.42 -3.3 1,228,509 + MMP_RS06395 0.58 -2.3 1,228,568 + MMP_RS06395 0.67 -2.6 1,228,603 + MMP_RS06395 0.73 -2.8 1,228,798 + +0.4 1,228,837 + +0.2 1,228,928 - -1.7 1,228,929 + -3.3 1,228,929 + -4.2 1,228,929 + -3.1 1,228,929 + -3.1 1,228,951 + -3.0 1,228,985 + -3.1 1,228,985 + -2.7 1,228,985 + -4.3 1,228,986 - -3.4 1,228,986 - -4.5 1,228,987 + -3.9 1,228,987 + -2.9 1,228,990 + -2.7
Or see this region's nucleotide sequence