Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4541 and BT4542 overlap by 68 nucleotides BT4542 and BT4543 overlap by 8 nucleotides BT4543 and BT4544 are separated by 43 nucleotides
BT4541: BT4541 - putative Type I restriction modification DNA specificity protein (NCBI ptt file), at 5,962,329 to 5,962,931
BT4541
BT4542: BT4542 - Type I restriction enzyme EcoR124II specificity protein (NCBI ptt file), at 5,962,864 to 5,963,418
BT4542
BT4543: BT4543 - putative type I restriction enzyme specificity protein (NCBI ptt file), at 5,963,411 to 5,964,556
BT4543
BT4544: BT4544 - transposase (NCBI ptt file), at 5,964,600 to 5,965,526
BT4544
Position (kb)
5963
5964
5965 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5962.418 kb on + strand, within BT4541 at 5962.419 kb on - strand, within BT4541 at 5962.440 kb on + strand, within BT4541 at 5962.464 kb on - strand, within BT4541 at 5962.468 kb on - strand, within BT4541 at 5962.469 kb on + strand, within BT4541 at 5962.475 kb on + strand, within BT4541 at 5962.476 kb on - strand, within BT4541 at 5962.525 kb on - strand, within BT4541 at 5962.526 kb on + strand, within BT4541 at 5962.556 kb on - strand, within BT4541 at 5962.559 kb on - strand, within BT4541 at 5962.564 kb on + strand, within BT4541 at 5962.587 kb on + strand, within BT4541 at 5962.588 kb on - strand, within BT4541 at 5962.598 kb on + strand, within BT4541 at 5962.598 kb on + strand, within BT4541 at 5962.601 kb on + strand, within BT4541 at 5962.609 kb on - strand, within BT4541 at 5962.623 kb on - strand, within BT4541 at 5962.623 kb on - strand, within BT4541 at 5962.688 kb on + strand, within BT4541 at 5962.892 kb on + strand at 5962.892 kb on + strand at 5962.899 kb on - strand at 5962.946 kb on - strand, within BT4542 at 5962.956 kb on + strand, within BT4542 at 5962.964 kb on - strand, within BT4542 at 5962.966 kb on - strand, within BT4542 at 5962.995 kb on + strand, within BT4542 at 5963.004 kb on - strand, within BT4542 at 5963.067 kb on - strand, within BT4542 at 5963.068 kb on + strand, within BT4542 at 5963.069 kb on - strand, within BT4542 at 5963.103 kb on + strand, within BT4542 at 5963.119 kb on + strand, within BT4542 at 5963.123 kb on + strand, within BT4542 at 5963.167 kb on - strand, within BT4542 at 5963.167 kb on - strand, within BT4542 at 5963.178 kb on + strand, within BT4542 at 5963.207 kb on - strand, within BT4542 at 5963.216 kb on - strand, within BT4542 at 5963.251 kb on + strand, within BT4542 at 5963.264 kb on + strand, within BT4542 at 5963.265 kb on - strand, within BT4542 at 5963.313 kb on - strand, within BT4542 at 5963.333 kb on + strand, within BT4542 at 5963.340 kb on + strand, within BT4542 at 5963.374 kb on + strand at 5963.375 kb on - strand at 5963.392 kb on + strand at 5963.393 kb on - strand at 5963.436 kb on + strand at 5963.437 kb on - strand at 5963.452 kb on + strand at 5963.471 kb on - strand at 5963.473 kb on + strand at 5963.496 kb on - strand at 5963.516 kb on + strand at 5963.549 kb on + strand, within BT4543 at 5963.570 kb on - strand, within BT4543 at 5963.570 kb on - strand, within BT4543 at 5963.610 kb on + strand, within BT4543 at 5963.654 kb on - strand, within BT4543 at 5963.724 kb on - strand, within BT4543 at 5963.800 kb on + strand, within BT4543 at 5963.800 kb on + strand, within BT4543 at 5963.832 kb on - strand, within BT4543 at 5963.843 kb on - strand, within BT4543 at 5963.875 kb on + strand, within BT4543 at 5963.884 kb on + strand, within BT4543 at 5963.956 kb on + strand, within BT4543 at 5964.009 kb on - strand, within BT4543 at 5964.031 kb on + strand, within BT4543 at 5964.163 kb on + strand, within BT4543 at 5964.163 kb on + strand, within BT4543 at 5964.164 kb on - strand, within BT4543 at 5964.164 kb on - strand, within BT4543 at 5964.195 kb on + strand, within BT4543 at 5964.258 kb on + strand, within BT4543 at 5964.377 kb on - strand, within BT4543 at 5964.391 kb on + strand, within BT4543 at 5964.409 kb on + strand, within BT4543 at 5964.410 kb on - strand, within BT4543 at 5964.582 kb on - strand at 5964.588 kb on - strand at 5964.631 kb on + strand at 5964.631 kb on + strand at 5964.657 kb on + strand at 5964.684 kb on + strand at 5964.684 kb on + strand at 5964.692 kb on - strand at 5964.870 kb on + strand, within BT4544 at 5964.892 kb on - strand, within BT4544 at 5964.929 kb on - strand, within BT4544 at 5965.099 kb on + strand, within BT4544 at 5965.108 kb on + strand, within BT4544 at 5965.108 kb on + strand, within BT4544 at 5965.111 kb on - strand, within BT4544 at 5965.302 kb on + strand, within BT4544 at 5965.303 kb on - strand, within BT4544 at 5965.303 kb on - strand, within BT4544 at 5965.315 kb on + strand, within BT4544 at 5965.316 kb on - strand, within BT4544 at 5965.384 kb on + strand, within BT4544 at 5965.458 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 5,962,418 + BT4541 0.15 -2.0 5,962,419 - BT4541 0.15 -0.0 5,962,440 + BT4541 0.18 -0.0 5,962,464 - BT4541 0.22 -1.0 5,962,468 - BT4541 0.23 -1.8 5,962,469 + BT4541 0.23 -1.6 5,962,475 + BT4541 0.24 -1.0 5,962,476 - BT4541 0.24 -0.4 5,962,525 - BT4541 0.33 -1.6 5,962,526 + BT4541 0.33 -0.3 5,962,556 - BT4541 0.38 -0.0 5,962,559 - BT4541 0.38 +0.8 5,962,564 + BT4541 0.39 -2.4 5,962,587 + BT4541 0.43 -0.0 5,962,588 - BT4541 0.43 -0.1 5,962,598 + BT4541 0.45 -1.6 5,962,598 + BT4541 0.45 -0.0 5,962,601 + BT4541 0.45 -0.2 5,962,609 - BT4541 0.46 -0.4 5,962,623 - BT4541 0.49 -0.7 5,962,623 - BT4541 0.49 -0.0 5,962,688 + BT4541 0.60 -0.0 5,962,892 + +1.5 5,962,892 + +1.0 5,962,899 - -0.6 5,962,946 - BT4542 0.15 +0.2 5,962,956 + BT4542 0.17 -0.9 5,962,964 - BT4542 0.18 -1.9 5,962,966 - BT4542 0.18 -0.4 5,962,995 + BT4542 0.24 +1.5 5,963,004 - BT4542 0.25 -1.7 5,963,067 - BT4542 0.37 -0.0 5,963,068 + BT4542 0.37 -0.5 5,963,069 - BT4542 0.37 -1.0 5,963,103 + BT4542 0.43 -0.5 5,963,119 + BT4542 0.46 +0.3 5,963,123 + BT4542 0.47 -1.8 5,963,167 - BT4542 0.55 -2.0 5,963,167 - BT4542 0.55 -2.0 5,963,178 + BT4542 0.57 -0.0 5,963,207 - BT4542 0.62 +0.4 5,963,216 - BT4542 0.63 -2.4 5,963,251 + BT4542 0.70 -3.1 5,963,264 + BT4542 0.72 -1.5 5,963,265 - BT4542 0.72 -0.0 5,963,313 - BT4542 0.81 +0.2 5,963,333 + BT4542 0.85 +2.0 5,963,340 + BT4542 0.86 -0.4 5,963,374 + -0.6 5,963,375 - -0.3 5,963,392 + -0.0 5,963,393 - -4.7 5,963,436 + -0.0 5,963,437 - -3.0 5,963,452 + -0.0 5,963,471 - -4.7 5,963,473 + +1.3 5,963,496 - -0.5 5,963,516 + -1.6 5,963,549 + BT4543 0.12 -2.0 5,963,570 - BT4543 0.14 -1.2 5,963,570 - BT4543 0.14 -0.0 5,963,610 + BT4543 0.17 +0.4 5,963,654 - BT4543 0.21 -0.0 5,963,724 - BT4543 0.27 -0.9 5,963,800 + BT4543 0.34 -0.7 5,963,800 + BT4543 0.34 -1.6 5,963,832 - BT4543 0.37 -0.0 5,963,843 - BT4543 0.38 -1.9 5,963,875 + BT4543 0.40 -0.5 5,963,884 + BT4543 0.41 -0.0 5,963,956 + BT4543 0.48 -0.0 5,964,009 - BT4543 0.52 +1.1 5,964,031 + BT4543 0.54 -1.6 5,964,163 + BT4543 0.66 +0.7 5,964,163 + BT4543 0.66 -1.9 5,964,164 - BT4543 0.66 -1.6 5,964,164 - BT4543 0.66 +0.1 5,964,195 + BT4543 0.68 -0.4 5,964,258 + BT4543 0.74 -0.8 5,964,377 - BT4543 0.84 +0.4 5,964,391 + BT4543 0.86 -1.0 5,964,409 + BT4543 0.87 -0.4 5,964,410 - BT4543 0.87 -0.5 5,964,582 - -0.0 5,964,588 - -2.4 5,964,631 + -0.6 5,964,631 + -2.0 5,964,657 + -0.4 5,964,684 + -2.4 5,964,684 + -1.3 5,964,692 - +0.4 5,964,870 + BT4544 0.29 -1.0 5,964,892 - BT4544 0.31 -1.7 5,964,929 - BT4544 0.35 -0.0 5,965,099 + BT4544 0.54 -0.0 5,965,108 + BT4544 0.55 -1.4 5,965,108 + BT4544 0.55 -2.6 5,965,111 - BT4544 0.55 +1.5 5,965,302 + BT4544 0.76 -2.5 5,965,303 - BT4544 0.76 -1.3 5,965,303 - BT4544 0.76 -1.1 5,965,315 + BT4544 0.77 -0.5 5,965,316 - BT4544 0.77 -1.2 5,965,384 + BT4544 0.85 +1.5 5,965,458 + -0.4
Or see this region's nucleotide sequence