Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4039 and BT4040 are separated by 39 nucleotides BT4040 and BT4041 are separated by 905 nucleotides
BT4039: BT4039 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 5,250,659 to 5,253,760
BT4039
BT4040: BT4040 - putative galactose oxidase precursor (NCBI ptt file), at 5,253,800 to 5,255,143
BT4040
BT4041: BT4041 - conserved hypothetical protein (NCBI ptt file), at 5,256,049 to 5,257,923
BT4041
Position (kb)
5253
5254
5255
5256 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5252.807 kb on + strand, within BT4039 at 5252.818 kb on + strand, within BT4039 at 5252.820 kb on + strand, within BT4039 at 5252.827 kb on + strand, within BT4039 at 5252.828 kb on - strand, within BT4039 at 5252.828 kb on - strand, within BT4039 at 5252.832 kb on + strand, within BT4039 at 5252.855 kb on - strand, within BT4039 at 5252.879 kb on + strand, within BT4039 at 5252.904 kb on + strand, within BT4039 at 5252.967 kb on + strand, within BT4039 at 5252.976 kb on - strand, within BT4039 at 5253.026 kb on - strand, within BT4039 at 5253.152 kb on - strand, within BT4039 at 5253.212 kb on + strand, within BT4039 at 5253.277 kb on - strand, within BT4039 at 5253.344 kb on - strand, within BT4039 at 5253.499 kb on + strand at 5253.500 kb on - strand at 5253.502 kb on - strand at 5253.548 kb on - strand at 5253.585 kb on + strand at 5253.585 kb on - strand at 5253.693 kb on + strand at 5253.704 kb on + strand at 5253.717 kb on - strand at 5253.786 kb on + strand at 5253.795 kb on + strand at 5253.818 kb on + strand at 5253.818 kb on + strand at 5253.818 kb on + strand at 5253.824 kb on + strand at 5253.824 kb on + strand at 5253.873 kb on + strand at 5253.874 kb on - strand at 5253.877 kb on + strand at 5254.013 kb on - strand, within BT4040 at 5254.040 kb on + strand, within BT4040 at 5254.079 kb on + strand, within BT4040 at 5254.080 kb on - strand, within BT4040 at 5254.087 kb on - strand, within BT4040 at 5254.097 kb on + strand, within BT4040 at 5254.097 kb on + strand, within BT4040 at 5254.136 kb on + strand, within BT4040 at 5254.243 kb on + strand, within BT4040 at 5254.244 kb on - strand, within BT4040 at 5254.444 kb on - strand, within BT4040 at 5254.458 kb on - strand, within BT4040 at 5254.463 kb on + strand, within BT4040 at 5254.468 kb on - strand, within BT4040 at 5254.470 kb on + strand, within BT4040 at 5254.471 kb on - strand, within BT4040 at 5254.491 kb on + strand, within BT4040 at 5254.579 kb on + strand, within BT4040 at 5254.588 kb on + strand, within BT4040 at 5254.608 kb on - strand, within BT4040 at 5254.731 kb on + strand, within BT4040 at 5254.736 kb on - strand, within BT4040 at 5254.865 kb on + strand, within BT4040 at 5254.869 kb on - strand, within BT4040 at 5254.875 kb on + strand, within BT4040 at 5254.977 kb on - strand, within BT4040 at 5255.056 kb on + strand at 5255.068 kb on + strand at 5255.100 kb on - strand at 5255.226 kb on - strand at 5255.418 kb on + strand at 5255.418 kb on + strand at 5255.429 kb on + strand at 5255.541 kb on - strand at 5255.546 kb on - strand at 5255.585 kb on + strand at 5255.591 kb on + strand at 5255.592 kb on - strand at 5255.611 kb on + strand at 5255.616 kb on + strand at 5255.621 kb on - strand at 5255.651 kb on + strand at 5255.696 kb on - strand at 5255.741 kb on + strand at 5255.741 kb on + strand at 5255.747 kb on + strand at 5255.768 kb on + strand at 5255.792 kb on + strand at 5255.815 kb on - strand at 5255.836 kb on + strand at 5255.837 kb on - strand at 5255.837 kb on - strand at 5255.838 kb on + strand at 5255.957 kb on + strand at 5255.958 kb on - strand at 5255.959 kb on + strand at 5256.014 kb on + strand at 5256.016 kb on + strand at 5256.029 kb on - strand at 5256.046 kb on + strand at 5256.047 kb on - strand at 5256.051 kb on - strand at 5256.098 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 5,252,807 + BT4039 0.69 -1.1 5,252,818 + BT4039 0.70 -1.9 5,252,820 + BT4039 0.70 -0.5 5,252,827 + BT4039 0.70 -1.1 5,252,828 - BT4039 0.70 -1.2 5,252,828 - BT4039 0.70 -0.3 5,252,832 + BT4039 0.70 -2.2 5,252,855 - BT4039 0.71 -0.1 5,252,879 + BT4039 0.72 -2.9 5,252,904 + BT4039 0.72 -1.7 5,252,967 + BT4039 0.74 -1.1 5,252,976 - BT4039 0.75 -2.4 5,253,026 - BT4039 0.76 -0.1 5,253,152 - BT4039 0.80 +0.5 5,253,212 + BT4039 0.82 -1.8 5,253,277 - BT4039 0.84 -0.8 5,253,344 - BT4039 0.87 -1.2 5,253,499 + -1.6 5,253,500 - +0.9 5,253,502 - -1.6 5,253,548 - -0.1 5,253,585 + -0.9 5,253,585 - -3.2 5,253,693 + -1.3 5,253,704 + -0.1 5,253,717 - -2.0 5,253,786 + -0.9 5,253,795 + -2.1 5,253,818 + -1.1 5,253,818 + +0.0 5,253,818 + -1.1 5,253,824 + -0.5 5,253,824 + -0.3 5,253,873 + -0.3 5,253,874 - -3.6 5,253,877 + -1.6 5,254,013 - BT4040 0.16 -1.6 5,254,040 + BT4040 0.18 -0.7 5,254,079 + BT4040 0.21 -0.1 5,254,080 - BT4040 0.21 -1.2 5,254,087 - BT4040 0.21 -0.1 5,254,097 + BT4040 0.22 -1.7 5,254,097 + BT4040 0.22 -1.3 5,254,136 + BT4040 0.25 -1.5 5,254,243 + BT4040 0.33 +0.8 5,254,244 - BT4040 0.33 -0.6 5,254,444 - BT4040 0.48 -2.4 5,254,458 - BT4040 0.49 -3.1 5,254,463 + BT4040 0.49 -2.9 5,254,468 - BT4040 0.50 -1.4 5,254,470 + BT4040 0.50 -1.6 5,254,471 - BT4040 0.50 -0.6 5,254,491 + BT4040 0.51 -0.6 5,254,579 + BT4040 0.58 -1.1 5,254,588 + BT4040 0.59 -1.6 5,254,608 - BT4040 0.60 -1.8 5,254,731 + BT4040 0.69 -0.2 5,254,736 - BT4040 0.70 -0.1 5,254,865 + BT4040 0.79 -0.1 5,254,869 - BT4040 0.80 -1.1 5,254,875 + BT4040 0.80 -0.6 5,254,977 - BT4040 0.88 -0.6 5,255,056 + -0.2 5,255,068 + -2.6 5,255,100 - -0.1 5,255,226 - -0.1 5,255,418 + -1.5 5,255,418 + -0.6 5,255,429 + -0.1 5,255,541 - -1.0 5,255,546 - -2.7 5,255,585 + -2.5 5,255,591 + -2.9 5,255,592 - -0.1 5,255,611 + -1.2 5,255,616 + -1.1 5,255,621 - -1.8 5,255,651 + +1.5 5,255,696 - -1.6 5,255,741 + -0.1 5,255,741 + -1.9 5,255,747 + +1.5 5,255,768 + -2.2 5,255,792 + -2.9 5,255,815 - +0.9 5,255,836 + +1.5 5,255,837 - -1.1 5,255,837 - -4.0 5,255,838 + -2.2 5,255,957 + -1.7 5,255,958 - -2.4 5,255,959 + -1.6 5,256,014 + -0.6 5,256,016 + +1.5 5,256,029 - +0.0 5,256,046 + +2.5 5,256,047 - -2.4 5,256,051 - +0.1 5,256,098 - +0.9
Or see this region's nucleotide sequence