Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3001 and BT3002 are separated by 219 nucleotides BT3002 and BT3003 are separated by 104 nucleotides
BT3001: BT3001 - ATP-dependent DNA helicase (NCBI ptt file), at 3,782,479 to 3,784,062
BT3001
BT3002: BT3002 - hypothetical protein (NCBI ptt file), at 3,784,282 to 3,784,485
BT3002
BT3003: BT3003 - hypothetical protein (NCBI ptt file), at 3,784,590 to 3,786,869
BT3003
Position (kb)
3784
3785 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3783.334 kb on - strand, within BT3001 at 3783.334 kb on - strand, within BT3001 at 3783.372 kb on - strand, within BT3001 at 3783.385 kb on + strand, within BT3001 at 3783.385 kb on + strand, within BT3001 at 3783.424 kb on - strand, within BT3001 at 3783.435 kb on + strand, within BT3001 at 3783.435 kb on + strand, within BT3001 at 3783.435 kb on + strand, within BT3001 at 3783.436 kb on - strand, within BT3001 at 3783.440 kb on - strand, within BT3001 at 3783.490 kb on + strand, within BT3001 at 3783.490 kb on + strand, within BT3001 at 3783.490 kb on + strand, within BT3001 at 3783.536 kb on - strand, within BT3001 at 3783.543 kb on + strand, within BT3001 at 3783.555 kb on + strand, within BT3001 at 3783.556 kb on - strand, within BT3001 at 3783.558 kb on + strand at 3783.558 kb on + strand, within BT3001 at 3783.558 kb on + strand, within BT3001 at 3783.559 kb on - strand, within BT3001 at 3783.559 kb on - strand, within BT3001 at 3783.601 kb on - strand, within BT3001 at 3783.741 kb on + strand, within BT3001 at 3783.741 kb on + strand, within BT3001 at 3783.844 kb on - strand, within BT3001 at 3783.856 kb on - strand, within BT3001 at 3783.856 kb on - strand, within BT3001 at 3783.856 kb on - strand, within BT3001 at 3783.871 kb on + strand, within BT3001 at 3783.909 kb on + strand at 3783.980 kb on + strand at 3783.980 kb on + strand at 3783.980 kb on + strand at 3783.980 kb on + strand at 3783.980 kb on + strand at 3783.981 kb on - strand at 3784.047 kb on + strand at 3784.047 kb on + strand at 3784.048 kb on - strand at 3784.051 kb on + strand at 3784.090 kb on + strand at 3784.090 kb on + strand at 3784.103 kb on - strand at 3784.120 kb on + strand at 3784.120 kb on + strand at 3784.121 kb on - strand at 3784.121 kb on - strand at 3784.124 kb on + strand at 3784.124 kb on + strand at 3784.178 kb on - strand at 3784.239 kb on - strand at 3784.311 kb on - strand, within BT3002 at 3784.311 kb on - strand, within BT3002 at 3784.314 kb on + strand, within BT3002 at 3784.315 kb on - strand, within BT3002 at 3784.321 kb on + strand, within BT3002 at 3784.328 kb on - strand, within BT3002 at 3784.330 kb on + strand, within BT3002 at 3784.358 kb on - strand, within BT3002 at 3784.362 kb on + strand, within BT3002 at 3784.398 kb on + strand, within BT3002 at 3784.401 kb on + strand, within BT3002 at 3784.467 kb on + strand at 3784.473 kb on + strand at 3784.491 kb on - strand at 3784.495 kb on + strand at 3784.495 kb on + strand at 3784.522 kb on + strand at 3784.524 kb on + strand at 3784.551 kb on + strand at 3784.563 kb on + strand at 3784.578 kb on + strand at 3784.589 kb on + strand at 3784.590 kb on - strand at 3784.612 kb on + strand at 3784.613 kb on - strand at 3784.620 kb on - strand at 3784.620 kb on - strand at 3784.626 kb on - strand at 3784.769 kb on - strand at 3784.848 kb on - strand, within BT3003 at 3784.869 kb on - strand, within BT3003 at 3784.869 kb on - strand, within BT3003 at 3784.870 kb on + strand, within BT3003 at 3784.936 kb on + strand, within BT3003 at 3784.964 kb on - strand, within BT3003 at 3785.012 kb on + strand, within BT3003 at 3785.019 kb on + strand, within BT3003 at 3785.020 kb on - strand, within BT3003 at 3785.063 kb on - strand, within BT3003 at 3785.085 kb on + strand, within BT3003 at 3785.085 kb on + strand, within BT3003 at 3785.085 kb on + strand, within BT3003 at 3785.086 kb on - strand, within BT3003 at 3785.097 kb on + strand, within BT3003 at 3785.097 kb on + strand, within BT3003 at 3785.098 kb on - strand, within BT3003 at 3785.099 kb on + strand, within BT3003 at 3785.116 kb on + strand, within BT3003 at 3785.116 kb on + strand, within BT3003 at 3785.150 kb on + strand, within BT3003 at 3785.150 kb on + strand, within BT3003 at 3785.151 kb on - strand, within BT3003 at 3785.239 kb on + strand, within BT3003 at 3785.239 kb on + strand, within BT3003 at 3785.250 kb on - strand, within BT3003 at 3785.283 kb on - strand, within BT3003 at 3785.283 kb on - strand, within BT3003 at 3785.292 kb on + strand, within BT3003 at 3785.292 kb on + strand, within BT3003 at 3785.301 kb on - strand, within BT3003 at 3785.341 kb on + strand, within BT3003 at 3785.342 kb on - strand, within BT3003 at 3785.346 kb on + strand, within BT3003 at 3785.346 kb on + strand, within BT3003 at 3785.346 kb on + strand, within BT3003 at 3785.352 kb on - strand, within BT3003 at 3785.360 kb on - strand, within BT3003 at 3785.380 kb on + strand, within BT3003 at 3785.383 kb on + strand, within BT3003 at 3785.407 kb on + strand, within BT3003 at 3785.413 kb on - strand, within BT3003 at 3785.413 kb on - strand, within BT3003 at 3785.423 kb on + strand, within BT3003 at 3785.438 kb on + strand, within BT3003 at 3785.447 kb on + strand, within BT3003
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 3,783,334 - BT3001 0.54 -1.7 3,783,334 - BT3001 0.54 -1.7 3,783,372 - BT3001 0.56 -1.1 3,783,385 + BT3001 0.57 -1.1 3,783,385 + BT3001 0.57 +0.9 3,783,424 - BT3001 0.60 -0.5 3,783,435 + BT3001 0.60 -1.5 3,783,435 + BT3001 0.60 -0.5 3,783,435 + BT3001 0.60 +1.9 3,783,436 - BT3001 0.60 -1.0 3,783,440 - BT3001 0.61 -2.5 3,783,490 + BT3001 0.64 +0.5 3,783,490 + BT3001 0.64 -1.5 3,783,490 + BT3001 0.64 -1.1 3,783,536 - BT3001 0.67 -3.9 3,783,543 + BT3001 0.67 -1.1 3,783,555 + BT3001 0.68 -1.5 3,783,556 - BT3001 0.68 -1.1 3,783,558 + +0.9 3,783,558 + BT3001 0.68 -0.3 3,783,558 + BT3001 0.68 -0.6 3,783,559 - BT3001 0.68 -2.7 3,783,559 - BT3001 0.68 -0.1 3,783,601 - BT3001 0.71 -1.0 3,783,741 + BT3001 0.80 -1.2 3,783,741 + BT3001 0.80 -0.5 3,783,844 - BT3001 0.86 -2.5 3,783,856 - BT3001 0.87 -0.1 3,783,856 - BT3001 0.87 -1.1 3,783,856 - BT3001 0.87 +0.9 3,783,871 + BT3001 0.88 -2.5 3,783,909 + -1.2 3,783,980 + -3.7 3,783,980 + +1.6 3,783,980 + -2.5 3,783,980 + -2.3 3,783,980 + -3.3 3,783,981 - -0.1 3,784,047 + -0.7 3,784,047 + -0.1 3,784,048 - -1.8 3,784,051 + +0.5 3,784,090 + -1.1 3,784,090 + -1.6 3,784,103 - -3.0 3,784,120 + -1.5 3,784,120 + -2.5 3,784,121 - -0.1 3,784,121 - -1.0 3,784,124 + -0.3 3,784,124 + -1.1 3,784,178 - -2.4 3,784,239 - -1.3 3,784,311 - BT3002 0.14 -2.9 3,784,311 - BT3002 0.14 -2.5 3,784,314 + BT3002 0.16 -0.5 3,784,315 - BT3002 0.16 -0.1 3,784,321 + BT3002 0.19 -2.5 3,784,328 - BT3002 0.23 -1.7 3,784,330 + BT3002 0.24 +2.0 3,784,358 - BT3002 0.37 -2.5 3,784,362 + BT3002 0.39 -2.3 3,784,398 + BT3002 0.57 -0.7 3,784,401 + BT3002 0.58 -2.6 3,784,467 + -1.5 3,784,473 + -2.1 3,784,491 - -1.5 3,784,495 + +1.2 3,784,495 + -1.0 3,784,522 + -2.5 3,784,524 + -0.1 3,784,551 + -1.1 3,784,563 + -0.7 3,784,578 + -2.5 3,784,589 + -0.8 3,784,590 - +2.0 3,784,612 + -1.5 3,784,613 - -0.7 3,784,620 - +0.7 3,784,620 - -3.5 3,784,626 - -2.5 3,784,769 - -3.9 3,784,848 - BT3003 0.11 -0.7 3,784,869 - BT3003 0.12 -0.1 3,784,869 - BT3003 0.12 -3.3 3,784,870 + BT3003 0.12 -2.7 3,784,936 + BT3003 0.15 -0.2 3,784,964 - BT3003 0.16 -0.1 3,785,012 + BT3003 0.19 -1.9 3,785,019 + BT3003 0.19 -1.6 3,785,020 - BT3003 0.19 -1.1 3,785,063 - BT3003 0.21 -1.7 3,785,085 + BT3003 0.22 -0.6 3,785,085 + BT3003 0.22 -1.1 3,785,085 + BT3003 0.22 -2.5 3,785,086 - BT3003 0.22 -1.4 3,785,097 + BT3003 0.22 -1.0 3,785,097 + BT3003 0.22 +0.9 3,785,098 - BT3003 0.22 -1.5 3,785,099 + BT3003 0.22 -1.5 3,785,116 + BT3003 0.23 -0.9 3,785,116 + BT3003 0.23 -1.7 3,785,150 + BT3003 0.25 -0.7 3,785,150 + BT3003 0.25 -0.5 3,785,151 - BT3003 0.25 +0.2 3,785,239 + BT3003 0.28 -0.8 3,785,239 + BT3003 0.28 -1.5 3,785,250 - BT3003 0.29 -3.9 3,785,283 - BT3003 0.30 -1.9 3,785,283 - BT3003 0.30 -0.1 3,785,292 + BT3003 0.31 +0.4 3,785,292 + BT3003 0.31 -1.9 3,785,301 - BT3003 0.31 -0.8 3,785,341 + BT3003 0.33 -3.9 3,785,342 - BT3003 0.33 -0.1 3,785,346 + BT3003 0.33 -0.9 3,785,346 + BT3003 0.33 -0.4 3,785,346 + BT3003 0.33 -3.2 3,785,352 - BT3003 0.33 -2.5 3,785,360 - BT3003 0.34 -3.6 3,785,380 + BT3003 0.35 -0.5 3,785,383 + BT3003 0.35 -1.1 3,785,407 + BT3003 0.36 -0.1 3,785,413 - BT3003 0.36 -1.0 3,785,413 - BT3003 0.36 -3.6 3,785,423 + BT3003 0.37 -0.1 3,785,438 + BT3003 0.37 -1.1 3,785,447 + BT3003 0.38 -1.7
Or see this region's nucleotide sequence