Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2831 and BT2832 overlap by 20 nucleotides BT2832 and BT2833 are separated by 3 nucleotides BT2833 and BT2834 are separated by 27 nucleotides
BT2831: BT2831 - rhomboid family protein (NCBI ptt file), at 3,529,445 to 3,530,119
BT2831
BT2832: BT2832 - rhomboid family protein (NCBI ptt file), at 3,530,100 to 3,531,002
BT2832
BT2833: BT2833 - hypothetical protein (NCBI ptt file), at 3,531,006 to 3,532,091
BT2833
BT2834: BT2834 - peptidyl-dipeptidase (NCBI ptt file), at 3,532,119 to 3,534,203
BT2834
Position (kb)
3531
3532
3533 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 3530.047 kb on + strand, within BT2831 at 3530.071 kb on + strand at 3530.071 kb on + strand at 3530.075 kb on + strand at 3531.064 kb on + strand at 3531.064 kb on + strand at 3531.087 kb on + strand at 3531.087 kb on + strand at 3531.087 kb on + strand at 3531.202 kb on - strand, within BT2833 at 3531.238 kb on + strand, within BT2833 at 3531.239 kb on - strand, within BT2833 at 3531.242 kb on + strand, within BT2833 at 3531.242 kb on + strand, within BT2833 at 3531.242 kb on + strand, within BT2833 at 3531.243 kb on - strand, within BT2833 at 3531.243 kb on - strand, within BT2833 at 3531.243 kb on - strand, within BT2833 at 3531.253 kb on + strand, within BT2833 at 3531.262 kb on - strand, within BT2833 at 3531.263 kb on + strand, within BT2833 at 3531.321 kb on + strand, within BT2833 at 3531.406 kb on + strand, within BT2833 at 3531.407 kb on - strand, within BT2833 at 3531.407 kb on - strand, within BT2833 at 3531.407 kb on - strand, within BT2833 at 3531.407 kb on - strand, within BT2833 at 3531.447 kb on + strand, within BT2833 at 3531.487 kb on - strand, within BT2833 at 3531.487 kb on - strand, within BT2833 at 3531.635 kb on + strand, within BT2833 at 3531.647 kb on - strand, within BT2833 at 3531.705 kb on - strand at 3531.705 kb on - strand, within BT2833 at 3531.705 kb on - strand, within BT2833 at 3531.846 kb on - strand, within BT2833 at 3531.883 kb on - strand, within BT2833 at 3531.883 kb on - strand, within BT2833 at 3532.075 kb on - strand at 3532.075 kb on - strand at 3532.077 kb on - strand at 3532.108 kb on + strand at 3532.109 kb on - strand at 3532.150 kb on + strand at 3532.150 kb on + strand at 3532.158 kb on - strand at 3532.164 kb on + strand at 3532.164 kb on + strand at 3532.165 kb on - strand at 3532.299 kb on + strand at 3532.337 kb on + strand, within BT2834 at 3532.337 kb on + strand, within BT2834 at 3532.350 kb on - strand, within BT2834 at 3532.393 kb on + strand, within BT2834 at 3532.393 kb on + strand, within BT2834 at 3532.394 kb on - strand, within BT2834 at 3532.394 kb on - strand, within BT2834 at 3532.412 kb on + strand, within BT2834 at 3532.537 kb on - strand, within BT2834 at 3532.537 kb on - strand, within BT2834 at 3532.596 kb on + strand, within BT2834 at 3532.597 kb on - strand, within BT2834 at 3532.623 kb on - strand, within BT2834 at 3532.700 kb on + strand, within BT2834 at 3532.701 kb on - strand, within BT2834 at 3532.705 kb on + strand, within BT2834 at 3532.872 kb on + strand, within BT2834 at 3532.914 kb on + strand, within BT2834 at 3532.914 kb on + strand, within BT2834 at 3533.068 kb on + strand, within BT2834 at 3533.068 kb on + strand, within BT2834
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 3,530,047 + BT2831 0.89 -0.7 3,530,071 + -2.1 3,530,071 + -2.5 3,530,075 + -4.2 3,531,064 + -2.1 3,531,064 + -2.7 3,531,087 + -3.6 3,531,087 + -2.1 3,531,087 + -2.7 3,531,202 - BT2833 0.18 -0.5 3,531,238 + BT2833 0.21 -0.3 3,531,239 - BT2833 0.21 +0.0 3,531,242 + BT2833 0.22 -1.7 3,531,242 + BT2833 0.22 -1.6 3,531,242 + BT2833 0.22 -2.1 3,531,243 - BT2833 0.22 -1.8 3,531,243 - BT2833 0.22 -1.0 3,531,243 - BT2833 0.22 +1.4 3,531,253 + BT2833 0.23 -1.3 3,531,262 - BT2833 0.24 -1.7 3,531,263 + BT2833 0.24 -3.1 3,531,321 + BT2833 0.29 -1.5 3,531,406 + BT2833 0.37 -1.1 3,531,407 - BT2833 0.37 +1.4 3,531,407 - BT2833 0.37 -0.4 3,531,407 - BT2833 0.37 -1.7 3,531,407 - BT2833 0.37 -1.1 3,531,447 + BT2833 0.41 -0.1 3,531,487 - BT2833 0.44 -0.6 3,531,487 - BT2833 0.44 -1.5 3,531,635 + BT2833 0.58 -0.1 3,531,647 - BT2833 0.59 -0.1 3,531,705 - -0.1 3,531,705 - BT2833 0.64 +1.4 3,531,705 - BT2833 0.64 -0.1 3,531,846 - BT2833 0.77 -0.8 3,531,883 - BT2833 0.81 -2.1 3,531,883 - BT2833 0.81 -0.1 3,532,075 - -1.7 3,532,075 - -1.7 3,532,077 - -2.7 3,532,108 + -0.6 3,532,109 - -1.5 3,532,150 + -2.5 3,532,150 + -1.7 3,532,158 - -0.1 3,532,164 + -0.1 3,532,164 + -1.7 3,532,165 - -2.3 3,532,299 + -2.1 3,532,337 + BT2834 0.10 -3.1 3,532,337 + BT2834 0.10 -1.0 3,532,350 - BT2834 0.11 -1.7 3,532,393 + BT2834 0.13 -2.7 3,532,393 + BT2834 0.13 -1.7 3,532,394 - BT2834 0.13 -0.7 3,532,394 - BT2834 0.13 -0.8 3,532,412 + BT2834 0.14 -1.1 3,532,537 - BT2834 0.20 -1.7 3,532,537 - BT2834 0.20 -2.6 3,532,596 + BT2834 0.23 +1.9 3,532,597 - BT2834 0.23 -0.5 3,532,623 - BT2834 0.24 -1.1 3,532,700 + BT2834 0.28 -1.2 3,532,701 - BT2834 0.28 -0.1 3,532,705 + BT2834 0.28 -0.9 3,532,872 + BT2834 0.36 -7.1 3,532,914 + BT2834 0.38 -1.5 3,532,914 + BT2834 0.38 -0.1 3,533,068 + BT2834 0.46 -3.5 3,533,068 + BT2834 0.46 -0.1
Or see this region's nucleotide sequence