Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2822 and BT2823 are separated by 27 nucleotides BT2823 and BT2824 are separated by 96 nucleotides
BT2822: BT2822 - conserved hypothetical protein (NCBI ptt file), at 3,512,273 to 3,513,751
BT2822
BT2823: BT2823 - hypothetical protein (NCBI ptt file), at 3,513,779 to 3,515,254
BT2823
BT2824: BT2824 - beta-glucanase precursor (NCBI ptt file), at 3,515,351 to 3,516,469
BT2824
Position (kb)
3513
3514
3515
3516 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3512.841 kb on + strand, within BT2822 at 3512.863 kb on - strand, within BT2822 at 3513.018 kb on + strand, within BT2822 at 3513.030 kb on + strand, within BT2822 at 3513.080 kb on - strand, within BT2822 at 3513.083 kb on - strand, within BT2822 at 3513.122 kb on - strand, within BT2822 at 3513.203 kb on - strand, within BT2822 at 3513.208 kb on + strand, within BT2822 at 3513.233 kb on - strand, within BT2822 at 3513.251 kb on + strand, within BT2822 at 3513.281 kb on - strand, within BT2822 at 3513.284 kb on + strand, within BT2822 at 3513.285 kb on - strand, within BT2822 at 3513.303 kb on - strand, within BT2822 at 3513.482 kb on + strand, within BT2822 at 3513.583 kb on - strand, within BT2822 at 3513.589 kb on - strand, within BT2822 at 3513.589 kb on - strand, within BT2822 at 3513.589 kb on - strand, within BT2822 at 3513.735 kb on - strand at 3513.749 kb on + strand at 3513.750 kb on - strand at 3513.765 kb on - strand at 3513.791 kb on - strand at 3513.813 kb on + strand at 3513.829 kb on - strand at 3513.904 kb on - strand at 3513.914 kb on + strand at 3513.914 kb on + strand at 3513.915 kb on - strand at 3513.915 kb on - strand at 3513.918 kb on + strand at 3513.919 kb on - strand at 3513.919 kb on - strand at 3514.059 kb on - strand, within BT2823 at 3514.117 kb on + strand, within BT2823 at 3514.171 kb on + strand, within BT2823 at 3514.182 kb on + strand, within BT2823 at 3514.246 kb on + strand, within BT2823 at 3514.330 kb on + strand, within BT2823 at 3514.383 kb on + strand, within BT2823 at 3514.390 kb on - strand, within BT2823 at 3514.443 kb on + strand, within BT2823 at 3514.481 kb on + strand, within BT2823 at 3514.482 kb on - strand, within BT2823 at 3514.482 kb on - strand, within BT2823 at 3514.525 kb on - strand, within BT2823 at 3514.525 kb on - strand, within BT2823 at 3514.601 kb on - strand, within BT2823 at 3514.638 kb on - strand, within BT2823 at 3514.646 kb on - strand, within BT2823 at 3514.717 kb on + strand, within BT2823 at 3514.718 kb on - strand, within BT2823 at 3514.721 kb on - strand, within BT2823 at 3514.721 kb on - strand, within BT2823 at 3514.832 kb on + strand, within BT2823 at 3514.834 kb on + strand, within BT2823 at 3514.835 kb on - strand, within BT2823 at 3514.835 kb on - strand, within BT2823 at 3514.837 kb on + strand, within BT2823 at 3514.838 kb on - strand, within BT2823 at 3514.841 kb on + strand, within BT2823 at 3514.841 kb on + strand, within BT2823 at 3514.842 kb on - strand, within BT2823 at 3514.871 kb on - strand, within BT2823 at 3514.885 kb on + strand, within BT2823 at 3514.885 kb on + strand, within BT2823 at 3514.886 kb on - strand, within BT2823 at 3514.948 kb on + strand, within BT2823 at 3514.972 kb on + strand, within BT2823 at 3515.006 kb on - strand, within BT2823 at 3515.063 kb on - strand, within BT2823 at 3515.102 kb on - strand, within BT2823 at 3515.153 kb on - strand at 3515.252 kb on + strand at 3515.261 kb on + strand at 3515.321 kb on + strand at 3515.367 kb on + strand at 3515.476 kb on - strand, within BT2824 at 3515.576 kb on - strand, within BT2824 at 3515.594 kb on + strand, within BT2824 at 3515.595 kb on - strand, within BT2824 at 3515.596 kb on + strand, within BT2824 at 3515.597 kb on - strand, within BT2824 at 3515.708 kb on + strand, within BT2824 at 3515.708 kb on + strand, within BT2824 at 3515.709 kb on - strand, within BT2824 at 3515.709 kb on - strand, within BT2824 at 3515.709 kb on - strand, within BT2824 at 3515.709 kb on - strand, within BT2824 at 3515.710 kb on + strand, within BT2824 at 3515.713 kb on + strand, within BT2824 at 3515.713 kb on + strand, within BT2824 at 3515.714 kb on - strand, within BT2824 at 3515.714 kb on - strand, within BT2824 at 3515.718 kb on + strand, within BT2824 at 3515.718 kb on + strand, within BT2824 at 3515.719 kb on - strand, within BT2824 at 3515.867 kb on + strand, within BT2824 at 3515.868 kb on - strand, within BT2824 at 3515.887 kb on + strand, within BT2824 at 3515.887 kb on + strand, within BT2824 at 3515.948 kb on - strand, within BT2824 at 3515.980 kb on - strand, within BT2824 at 3515.999 kb on + strand, within BT2824 at 3516.044 kb on + strand, within BT2824 at 3516.044 kb on + strand, within BT2824 at 3516.045 kb on - strand, within BT2824 at 3516.046 kb on + strand, within BT2824 at 3516.121 kb on + strand, within BT2824 at 3516.130 kb on + strand, within BT2824 at 3516.212 kb on + strand, within BT2824
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 3,512,841 + BT2822 0.38 -1.5 3,512,863 - BT2822 0.40 -2.1 3,513,018 + BT2822 0.50 +0.9 3,513,030 + BT2822 0.51 +2.7 3,513,080 - BT2822 0.55 -2.5 3,513,083 - BT2822 0.55 -3.8 3,513,122 - BT2822 0.57 -1.5 3,513,203 - BT2822 0.63 -0.4 3,513,208 + BT2822 0.63 -0.7 3,513,233 - BT2822 0.65 -2.9 3,513,251 + BT2822 0.66 -2.1 3,513,281 - BT2822 0.68 -0.3 3,513,284 + BT2822 0.68 -0.1 3,513,285 - BT2822 0.68 -2.5 3,513,303 - BT2822 0.70 -4.2 3,513,482 + BT2822 0.82 -2.1 3,513,583 - BT2822 0.89 -1.1 3,513,589 - BT2822 0.89 -2.1 3,513,589 - BT2822 0.89 -1.1 3,513,589 - BT2822 0.89 -0.9 3,513,735 - -1.1 3,513,749 + -1.9 3,513,750 - -2.5 3,513,765 - -0.9 3,513,791 - -1.2 3,513,813 + -0.1 3,513,829 - +0.9 3,513,904 - -2.7 3,513,914 + -1.1 3,513,914 + -0.5 3,513,915 - -1.3 3,513,915 - -1.1 3,513,918 + +0.9 3,513,919 - -1.8 3,513,919 - -1.5 3,514,059 - BT2823 0.19 -2.4 3,514,117 + BT2823 0.23 -0.5 3,514,171 + BT2823 0.27 -0.1 3,514,182 + BT2823 0.27 -1.0 3,514,246 + BT2823 0.32 -2.5 3,514,330 + BT2823 0.37 -0.1 3,514,383 + BT2823 0.41 -1.5 3,514,390 - BT2823 0.41 -0.4 3,514,443 + BT2823 0.45 -3.7 3,514,481 + BT2823 0.48 -2.1 3,514,482 - BT2823 0.48 -2.3 3,514,482 - BT2823 0.48 -0.8 3,514,525 - BT2823 0.51 -0.9 3,514,525 - BT2823 0.51 -0.5 3,514,601 - BT2823 0.56 -1.7 3,514,638 - BT2823 0.58 -2.5 3,514,646 - BT2823 0.59 -3.3 3,514,717 + BT2823 0.64 -0.1 3,514,718 - BT2823 0.64 -2.1 3,514,721 - BT2823 0.64 -0.1 3,514,721 - BT2823 0.64 -0.1 3,514,832 + BT2823 0.71 -1.3 3,514,834 + BT2823 0.71 -1.1 3,514,835 - BT2823 0.72 +0.5 3,514,835 - BT2823 0.72 -2.5 3,514,837 + BT2823 0.72 -3.6 3,514,838 - BT2823 0.72 -3.0 3,514,841 + BT2823 0.72 -0.5 3,514,841 + BT2823 0.72 -0.9 3,514,842 - BT2823 0.72 -2.5 3,514,871 - BT2823 0.74 -0.5 3,514,885 + BT2823 0.75 -1.1 3,514,885 + BT2823 0.75 -1.7 3,514,886 - BT2823 0.75 -0.9 3,514,948 + BT2823 0.79 -0.5 3,514,972 + BT2823 0.81 -1.1 3,515,006 - BT2823 0.83 -2.6 3,515,063 - BT2823 0.87 -2.1 3,515,102 - BT2823 0.90 -1.1 3,515,153 - -1.7 3,515,252 + -2.1 3,515,261 + -1.1 3,515,321 + -3.5 3,515,367 + -1.7 3,515,476 - BT2824 0.11 -1.1 3,515,576 - BT2824 0.20 -0.0 3,515,594 + BT2824 0.22 +0.5 3,515,595 - BT2824 0.22 +0.9 3,515,596 + BT2824 0.22 -0.7 3,515,597 - BT2824 0.22 -3.1 3,515,708 + BT2824 0.32 -0.4 3,515,708 + BT2824 0.32 -1.9 3,515,709 - BT2824 0.32 -0.9 3,515,709 - BT2824 0.32 -1.9 3,515,709 - BT2824 0.32 -0.9 3,515,709 - BT2824 0.32 -0.1 3,515,710 + BT2824 0.32 -3.1 3,515,713 + BT2824 0.32 +0.3 3,515,713 + BT2824 0.32 -1.7 3,515,714 - BT2824 0.32 -0.2 3,515,714 - BT2824 0.32 +0.9 3,515,718 + BT2824 0.33 -1.7 3,515,718 + BT2824 0.33 -2.0 3,515,719 - BT2824 0.33 +0.3 3,515,867 + BT2824 0.46 -0.9 3,515,868 - BT2824 0.46 -2.1 3,515,887 + BT2824 0.48 +0.2 3,515,887 + BT2824 0.48 -2.1 3,515,948 - BT2824 0.53 -1.0 3,515,980 - BT2824 0.56 -0.1 3,515,999 + BT2824 0.58 +0.9 3,516,044 + BT2824 0.62 -2.1 3,516,044 + BT2824 0.62 -0.9 3,516,045 - BT2824 0.62 -0.6 3,516,046 + BT2824 0.62 +0.9 3,516,121 + BT2824 0.69 +1.5 3,516,130 + BT2824 0.70 -2.7 3,516,212 + BT2824 0.77 -3.1
Or see this region's nucleotide sequence