Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2131 and BT2132 are separated by 393 nucleotides BT2132 and BT2133 overlap by 4 nucleotides
BT2131: BT2131 - hypothetical protein (NCBI ptt file), at 2,683,046 to 2,683,396
BT2131
BT2132: BT2132 - conserved hypothetical protein (NCBI ptt file), at 2,683,790 to 2,684,371
BT2132
BT2133: BT2133 - conserved hypothetical protein (NCBI ptt file), at 2,684,368 to 2,687,076
BT2133
Position (kb)
2683
2684
2685 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2682.827 kb on - strand at 2682.874 kb on + strand at 2682.875 kb on - strand at 2682.875 kb on - strand at 2682.890 kb on + strand at 2682.911 kb on - strand at 2682.921 kb on - strand at 2682.990 kb on - strand at 2683.106 kb on + strand, within BT2131 at 2683.132 kb on - strand, within BT2131 at 2683.132 kb on - strand, within BT2131 at 2683.136 kb on - strand, within BT2131 at 2683.144 kb on + strand, within BT2131 at 2683.192 kb on + strand, within BT2131 at 2683.192 kb on + strand, within BT2131 at 2683.214 kb on + strand, within BT2131 at 2683.215 kb on - strand, within BT2131 at 2683.215 kb on - strand, within BT2131 at 2683.274 kb on - strand, within BT2131 at 2683.290 kb on - strand, within BT2131 at 2683.301 kb on + strand, within BT2131 at 2683.304 kb on + strand, within BT2131 at 2683.304 kb on + strand, within BT2131 at 2683.304 kb on + strand, within BT2131 at 2683.305 kb on - strand, within BT2131 at 2683.306 kb on + strand, within BT2131 at 2683.361 kb on - strand, within BT2131 at 2683.394 kb on + strand at 2683.500 kb on - strand at 2683.508 kb on + strand at 2683.557 kb on - strand at 2683.557 kb on - strand at 2683.622 kb on + strand at 2683.626 kb on + strand at 2683.633 kb on + strand at 2683.634 kb on - strand at 2683.653 kb on + strand at 2683.690 kb on + strand at 2683.711 kb on + strand at 2683.711 kb on + strand at 2683.712 kb on - strand at 2683.749 kb on - strand at 2683.790 kb on - strand at 2683.795 kb on + strand at 2683.795 kb on + strand at 2683.845 kb on - strand at 2683.922 kb on - strand, within BT2132 at 2683.963 kb on + strand, within BT2132 at 2683.964 kb on - strand, within BT2132 at 2683.966 kb on - strand, within BT2132 at 2683.977 kb on + strand, within BT2132 at 2683.985 kb on + strand, within BT2132 at 2683.999 kb on + strand, within BT2132 at 2684.068 kb on + strand, within BT2132 at 2684.089 kb on - strand, within BT2132 at 2684.115 kb on + strand, within BT2132 at 2684.116 kb on - strand, within BT2132 at 2684.145 kb on + strand, within BT2132 at 2684.169 kb on + strand, within BT2132 at 2684.169 kb on + strand, within BT2132 at 2684.183 kb on - strand, within BT2132 at 2684.206 kb on - strand, within BT2132 at 2684.221 kb on - strand, within BT2132 at 2684.245 kb on + strand, within BT2132 at 2684.245 kb on + strand, within BT2132 at 2684.245 kb on + strand, within BT2132 at 2684.246 kb on - strand, within BT2132 at 2684.247 kb on + strand, within BT2132 at 2684.248 kb on - strand, within BT2132 at 2684.248 kb on - strand, within BT2132 at 2684.248 kb on - strand, within BT2132 at 2684.335 kb on - strand at 2684.369 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 2,682,827 - -0.7 2,682,874 + -1.5 2,682,875 - -0.7 2,682,875 - -0.8 2,682,890 + -0.0 2,682,911 - +0.0 2,682,921 - -2.1 2,682,990 - -1.5 2,683,106 + BT2131 0.17 -0.5 2,683,132 - BT2131 0.25 -2.8 2,683,132 - BT2131 0.25 +0.0 2,683,136 - BT2131 0.26 -1.3 2,683,144 + BT2131 0.28 +1.0 2,683,192 + BT2131 0.42 +0.0 2,683,192 + BT2131 0.42 -2.5 2,683,214 + BT2131 0.48 +0.0 2,683,215 - BT2131 0.48 +0.0 2,683,215 - BT2131 0.48 -1.5 2,683,274 - BT2131 0.65 -1.5 2,683,290 - BT2131 0.70 -0.5 2,683,301 + BT2131 0.73 -2.0 2,683,304 + BT2131 0.74 +1.0 2,683,304 + BT2131 0.74 -2.4 2,683,304 + BT2131 0.74 -1.5 2,683,305 - BT2131 0.74 +1.0 2,683,306 + BT2131 0.74 +0.8 2,683,361 - BT2131 0.90 +0.0 2,683,394 + -2.5 2,683,500 - -2.0 2,683,508 + +0.0 2,683,557 - -1.5 2,683,557 - -2.4 2,683,622 + -1.5 2,683,626 + +0.0 2,683,633 + -1.0 2,683,634 - -2.0 2,683,653 + -1.0 2,683,690 + +1.6 2,683,711 + -3.9 2,683,711 + -1.0 2,683,712 - +0.0 2,683,749 - -1.5 2,683,790 - -3.8 2,683,795 + +0.7 2,683,795 + -2.1 2,683,845 - -2.0 2,683,922 - BT2132 0.23 +0.0 2,683,963 + BT2132 0.30 -1.0 2,683,964 - BT2132 0.30 -2.3 2,683,966 - BT2132 0.30 -1.0 2,683,977 + BT2132 0.32 -1.5 2,683,985 + BT2132 0.34 -1.0 2,683,999 + BT2132 0.36 -4.1 2,684,068 + BT2132 0.48 -3.4 2,684,089 - BT2132 0.51 -1.2 2,684,115 + BT2132 0.56 +0.0 2,684,116 - BT2132 0.56 -1.5 2,684,145 + BT2132 0.61 +0.0 2,684,169 + BT2132 0.65 -2.2 2,684,169 + BT2132 0.65 -0.4 2,684,183 - BT2132 0.68 +0.0 2,684,206 - BT2132 0.71 -0.7 2,684,221 - BT2132 0.74 -2.8 2,684,245 + BT2132 0.78 -2.3 2,684,245 + BT2132 0.78 -0.5 2,684,245 + BT2132 0.78 -2.3 2,684,246 - BT2132 0.78 +0.0 2,684,247 + BT2132 0.79 -1.7 2,684,248 - BT2132 0.79 +0.0 2,684,248 - BT2132 0.79 -2.3 2,684,248 - BT2132 0.79 -1.0 2,684,335 - -0.3 2,684,369 + +0.0
Or see this region's nucleotide sequence