Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2116 and BT2117 are separated by 489 nucleotides BT2117 and BT2118 overlap by 4 nucleotides
BT2116: BT2116 - conserved hypothetical protein (NCBI ptt file), at 2,661,984 to 2,662,535
BT2116
BT2117: BT2117 - outer membrane protein, putative (NCBI ptt file), at 2,663,025 to 2,664,392
BT2117
BT2118: BT2118 - transporter, AcrB/D/F family (NCBI ptt file), at 2,664,389 to 2,667,529
BT2118
Position (kb)
2663
2664
2665 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2662.076 kb on - strand, within BT2116 at 2662.142 kb on + strand, within BT2116 at 2662.157 kb on + strand, within BT2116 at 2662.195 kb on - strand, within BT2116 at 2662.237 kb on + strand, within BT2116 at 2662.508 kb on - strand at 2662.508 kb on - strand at 2662.508 kb on - strand at 2662.508 kb on - strand at 2662.521 kb on + strand at 2662.536 kb on - strand at 2662.569 kb on + strand at 2662.570 kb on - strand at 2662.698 kb on + strand at 2662.740 kb on - strand at 2662.782 kb on + strand at 2662.783 kb on - strand at 2662.783 kb on - strand at 2662.783 kb on - strand at 2662.783 kb on - strand at 2662.784 kb on + strand at 2662.784 kb on + strand at 2662.785 kb on - strand at 2662.785 kb on - strand at 2662.785 kb on - strand at 2662.804 kb on + strand at 2662.856 kb on - strand at 2662.889 kb on + strand at 2662.891 kb on + strand at 2662.891 kb on + strand at 2662.905 kb on + strand at 2662.927 kb on - strand at 2662.949 kb on - strand at 2663.027 kb on - strand at 2663.054 kb on - strand at 2663.063 kb on - strand at 2663.071 kb on + strand at 2663.072 kb on - strand at 2663.104 kb on + strand at 2663.105 kb on - strand at 2663.167 kb on + strand at 2663.168 kb on - strand, within BT2117 at 2663.168 kb on - strand, within BT2117 at 2663.190 kb on - strand, within BT2117 at 2663.256 kb on - strand, within BT2117 at 2663.352 kb on - strand, within BT2117 at 2663.352 kb on - strand, within BT2117 at 2663.357 kb on + strand, within BT2117 at 2663.357 kb on + strand, within BT2117 at 2663.358 kb on - strand, within BT2117 at 2663.383 kb on - strand, within BT2117 at 2663.459 kb on - strand, within BT2117 at 2663.464 kb on + strand, within BT2117 at 2663.630 kb on + strand, within BT2117 at 2663.660 kb on + strand, within BT2117 at 2663.663 kb on - strand, within BT2117 at 2663.733 kb on - strand, within BT2117 at 2663.831 kb on - strand, within BT2117 at 2663.878 kb on + strand, within BT2117 at 2663.879 kb on - strand, within BT2117 at 2663.992 kb on + strand, within BT2117 at 2664.012 kb on + strand, within BT2117 at 2664.025 kb on + strand, within BT2117 at 2664.073 kb on + strand, within BT2117 at 2664.118 kb on - strand, within BT2117 at 2664.165 kb on - strand, within BT2117 at 2664.166 kb on + strand, within BT2117 at 2664.166 kb on + strand, within BT2117 at 2664.167 kb on - strand, within BT2117 at 2664.189 kb on - strand, within BT2117 at 2664.212 kb on + strand, within BT2117 at 2664.213 kb on - strand, within BT2117 at 2664.214 kb on + strand, within BT2117 at 2664.278 kb on - strand at 2664.484 kb on + strand at 2664.485 kb on - strand at 2664.508 kb on + strand at 2664.509 kb on - strand at 2664.541 kb on + strand at 2664.541 kb on + strand at 2664.541 kb on + strand at 2664.676 kb on + strand at 2664.801 kb on + strand, within BT2118 at 2664.817 kb on + strand, within BT2118 at 2664.817 kb on + strand, within BT2118 at 2664.817 kb on + strand, within BT2118 at 2664.818 kb on - strand, within BT2118 at 2664.818 kb on - strand, within BT2118 at 2664.841 kb on + strand, within BT2118 at 2664.877 kb on + strand, within BT2118 at 2664.920 kb on - strand, within BT2118 at 2665.266 kb on + strand, within BT2118 at 2665.270 kb on + strand, within BT2118 at 2665.343 kb on + strand, within BT2118 at 2665.343 kb on + strand, within BT2118 at 2665.343 kb on + strand, within BT2118 at 2665.343 kb on + strand, within BT2118 at 2665.344 kb on - strand, within BT2118 at 2665.344 kb on - strand, within BT2118 at 2665.389 kb on + strand, within BT2118 at 2665.390 kb on - strand, within BT2118
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 2,662,076 - BT2116 0.17 -2.6 2,662,142 + BT2116 0.29 -0.6 2,662,157 + BT2116 0.31 -1.0 2,662,195 - BT2116 0.38 +0.0 2,662,237 + BT2116 0.46 -0.1 2,662,508 - -1.1 2,662,508 - +1.6 2,662,508 - +1.8 2,662,508 - -0.3 2,662,521 + -2.1 2,662,536 - -1.0 2,662,569 + -0.4 2,662,570 - +0.0 2,662,698 + +1.0 2,662,740 - -1.4 2,662,782 + -2.3 2,662,783 - -0.6 2,662,783 - -1.0 2,662,783 - -1.1 2,662,783 - +1.0 2,662,784 + +0.0 2,662,784 + +0.6 2,662,785 - -1.1 2,662,785 - -0.6 2,662,785 - -2.6 2,662,804 + -1.6 2,662,856 - -1.0 2,662,889 + +0.0 2,662,891 + -1.6 2,662,891 + -1.0 2,662,905 + -0.4 2,662,927 - +1.3 2,662,949 - +0.0 2,663,027 - -2.1 2,663,054 - +1.6 2,663,063 - -1.3 2,663,071 + -2.0 2,663,072 - -0.7 2,663,104 + -2.3 2,663,105 - -0.9 2,663,167 + +0.0 2,663,168 - BT2117 0.10 +0.0 2,663,168 - BT2117 0.10 +1.3 2,663,190 - BT2117 0.12 -0.6 2,663,256 - BT2117 0.17 -2.3 2,663,352 - BT2117 0.24 -1.0 2,663,352 - BT2117 0.24 +0.0 2,663,357 + BT2117 0.24 -3.0 2,663,357 + BT2117 0.24 -4.1 2,663,358 - BT2117 0.24 -1.0 2,663,383 - BT2117 0.26 -1.7 2,663,459 - BT2117 0.32 -2.6 2,663,464 + BT2117 0.32 -0.4 2,663,630 + BT2117 0.44 +0.0 2,663,660 + BT2117 0.46 -2.4 2,663,663 - BT2117 0.47 +1.0 2,663,733 - BT2117 0.52 -2.3 2,663,831 - BT2117 0.59 -0.7 2,663,878 + BT2117 0.62 -1.6 2,663,879 - BT2117 0.62 +0.2 2,663,992 + BT2117 0.71 -0.7 2,664,012 + BT2117 0.72 +1.4 2,664,025 + BT2117 0.73 -2.0 2,664,073 + BT2117 0.77 -0.9 2,664,118 - BT2117 0.80 +0.7 2,664,165 - BT2117 0.83 +0.0 2,664,166 + BT2117 0.83 -0.0 2,664,166 + BT2117 0.83 -2.3 2,664,167 - BT2117 0.83 +0.0 2,664,189 - BT2117 0.85 +0.0 2,664,212 + BT2117 0.87 -1.1 2,664,213 - BT2117 0.87 -0.6 2,664,214 + BT2117 0.87 -1.0 2,664,278 - -0.2 2,664,484 + +0.0 2,664,485 - -0.7 2,664,508 + -2.6 2,664,509 - -2.6 2,664,541 + -1.0 2,664,541 + -2.3 2,664,541 + -3.6 2,664,676 + -1.0 2,664,801 + BT2118 0.13 +0.0 2,664,817 + BT2118 0.14 -1.0 2,664,817 + BT2118 0.14 -1.8 2,664,817 + BT2118 0.14 -3.1 2,664,818 - BT2118 0.14 -2.0 2,664,818 - BT2118 0.14 -0.6 2,664,841 + BT2118 0.14 -0.6 2,664,877 + BT2118 0.16 -0.7 2,664,920 - BT2118 0.17 +0.6 2,665,266 + BT2118 0.28 -1.6 2,665,270 + BT2118 0.28 -2.8 2,665,343 + BT2118 0.30 -2.0 2,665,343 + BT2118 0.30 +0.0 2,665,343 + BT2118 0.30 -0.9 2,665,343 + BT2118 0.30 -1.0 2,665,344 - BT2118 0.30 +2.0 2,665,344 - BT2118 0.30 +0.0 2,665,389 + BT2118 0.32 -1.0 2,665,390 - BT2118 0.32 -2.4
Or see this region's nucleotide sequence