Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1585 and BT1586 are separated by 80 nucleotides BT1586 and BT1587 are separated by 51 nucleotides BT1587 and BT1588 are separated by 88 nucleotides BT1588 and BT1589 are separated by 390 nucleotides
BT1585: BT1585 - hypothetical protein (NCBI ptt file), at 1,947,429 to 1,948,163
BT1585
BT1586: BT1586 - hypothetical protein (NCBI ptt file), at 1,948,244 to 1,948,816
BT1586
BT1587: BT1587 - conserved hypothetical protein (NCBI ptt file), at 1,948,868 to 1,949,620
BT1587
BT1588: BT1588 - conserved hypothetical protein (NCBI ptt file), at 1,949,709 to 1,950,179
BT1588
BT1589: BT1589 - hypothetical protein (NCBI ptt file), at 1,950,570 to 1,950,809
BT1589
Position (kb) 1948
1949
1950 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1947.886 kb on + strand, within BT1585 at 1947.937 kb on + strand, within BT1585 at 1947.991 kb on + strand, within BT1585 at 1948.024 kb on - strand, within BT1585 at 1948.171 kb on + strand at 1948.171 kb on + strand at 1948.219 kb on + strand at 1948.226 kb on - strand at 1948.236 kb on + strand at 1948.254 kb on - strand at 1948.254 kb on - strand at 1948.308 kb on + strand, within BT1586 at 1948.435 kb on - strand, within BT1586 at 1948.435 kb on - strand, within BT1586 at 1948.462 kb on - strand, within BT1586 at 1948.501 kb on + strand, within BT1586 at 1948.502 kb on - strand, within BT1586 at 1948.502 kb on - strand, within BT1586 at 1948.533 kb on - strand, within BT1586 at 1948.622 kb on + strand, within BT1586 at 1948.644 kb on + strand, within BT1586 at 1948.644 kb on + strand, within BT1586 at 1948.644 kb on + strand, within BT1586 at 1948.645 kb on - strand, within BT1586 at 1948.645 kb on - strand, within BT1586 at 1948.650 kb on + strand, within BT1586 at 1948.651 kb on - strand, within BT1586 at 1948.851 kb on + strand at 1948.851 kb on + strand at 1948.866 kb on - strand at 1948.905 kb on - strand at 1948.993 kb on + strand, within BT1587 at 1949.007 kb on - strand, within BT1587 at 1949.037 kb on + strand, within BT1587 at 1949.088 kb on + strand, within BT1587 at 1949.192 kb on - strand, within BT1587 at 1949.278 kb on + strand, within BT1587 at 1949.278 kb on + strand, within BT1587 at 1949.322 kb on + strand, within BT1587 at 1949.356 kb on + strand, within BT1587 at 1949.394 kb on + strand, within BT1587 at 1949.394 kb on + strand, within BT1587 at 1949.468 kb on + strand, within BT1587 at 1949.495 kb on + strand, within BT1587 at 1949.557 kb on + strand at 1949.568 kb on - strand at 1949.641 kb on - strand at 1949.694 kb on - strand at 1949.699 kb on - strand at 1949.773 kb on - strand, within BT1588 at 1949.773 kb on - strand, within BT1588 at 1949.867 kb on + strand, within BT1588 at 1950.066 kb on + strand, within BT1588 at 1950.067 kb on - strand, within BT1588 at 1950.069 kb on - strand, within BT1588 at 1950.191 kb on + strand at 1950.192 kb on - strand at 1950.298 kb on + strand at 1950.299 kb on - strand at 1950.303 kb on - strand at 1950.303 kb on - strand at 1950.336 kb on + strand at 1950.437 kb on - strand at 1950.518 kb on - strand at 1950.518 kb on - strand at 1950.524 kb on + strand at 1950.524 kb on + strand at 1950.528 kb on + strand at 1950.528 kb on + strand at 1950.528 kb on + strand at 1950.571 kb on + strand at 1950.620 kb on + strand, within BT1589
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 1,947,886 + BT1585 0.62 -1.8 1,947,937 + BT1585 0.69 -0.6 1,947,991 + BT1585 0.76 +0.4 1,948,024 - BT1585 0.81 +0.4 1,948,171 + -1.4 1,948,171 + -1.6 1,948,219 + -1.6 1,948,226 - -0.9 1,948,236 + -0.8 1,948,254 - -0.4 1,948,254 - -2.0 1,948,308 + BT1586 0.11 +0.0 1,948,435 - BT1586 0.33 -0.2 1,948,435 - BT1586 0.33 +0.0 1,948,462 - BT1586 0.38 -1.0 1,948,501 + BT1586 0.45 -0.6 1,948,502 - BT1586 0.45 -1.7 1,948,502 - BT1586 0.45 -0.7 1,948,533 - BT1586 0.50 +1.2 1,948,622 + BT1586 0.66 -1.4 1,948,644 + BT1586 0.70 -0.6 1,948,644 + BT1586 0.70 -2.3 1,948,644 + BT1586 0.70 -1.3 1,948,645 - BT1586 0.70 -1.0 1,948,645 - BT1586 0.70 +1.0 1,948,650 + BT1586 0.71 -2.0 1,948,651 - BT1586 0.71 -2.4 1,948,851 + -2.3 1,948,851 + -0.5 1,948,866 - -2.0 1,948,905 - -2.8 1,948,993 + BT1587 0.17 +0.0 1,949,007 - BT1587 0.18 +1.3 1,949,037 + BT1587 0.22 -0.3 1,949,088 + BT1587 0.29 +1.6 1,949,192 - BT1587 0.43 +0.0 1,949,278 + BT1587 0.54 +0.0 1,949,278 + BT1587 0.54 +2.0 1,949,322 + BT1587 0.60 +0.6 1,949,356 + BT1587 0.65 -0.6 1,949,394 + BT1587 0.70 -1.3 1,949,394 + BT1587 0.70 -2.8 1,949,468 + BT1587 0.80 -1.0 1,949,495 + BT1587 0.83 -0.9 1,949,557 + -2.0 1,949,568 - +1.0 1,949,641 - -1.9 1,949,694 - +0.0 1,949,699 - -1.6 1,949,773 - BT1588 0.14 -1.6 1,949,773 - BT1588 0.14 -2.0 1,949,867 + BT1588 0.34 -0.6 1,950,066 + BT1588 0.76 -1.8 1,950,067 - BT1588 0.76 -0.3 1,950,069 - BT1588 0.76 -3.4 1,950,191 + -3.2 1,950,192 - +0.0 1,950,298 + +0.3 1,950,299 - -1.0 1,950,303 - -1.0 1,950,303 - +0.0 1,950,336 + -1.0 1,950,437 - +0.0 1,950,518 - -0.7 1,950,518 - -0.9 1,950,524 + -0.6 1,950,524 + -0.3 1,950,528 + +0.0 1,950,528 + -3.0 1,950,528 + -1.2 1,950,571 + +0.0 1,950,620 + BT1589 0.21 -3.3
Or see this region's nucleotide sequence