Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0161 and BT0162 are separated by 19 nucleotides BT0162 and BT0163 are separated by 45 nucleotides BT0163 and BT0164 are separated by 7 nucleotides
BT0161: BT0161 - putative permease (NCBI ptt file), at 166,150 to 167,325
BT0161
BT0162: BT0162 - penicillin-binding protein 1C (PBP-1c) (NCBI ptt file), at 167,345 to 169,663
BT0162
BT0163: BT0163 - conserved hypothetical protein (NCBI ptt file), at 169,709 to 169,975
BT0163
BT0164: BT0164 - conserved hypothetical protein (NCBI ptt file), at 169,983 to 175,358
BT0164
Position (kb) 167
168
169
170 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 166.374 kb on + strand, within BT0161 at 166.379 kb on - strand, within BT0161 at 166.508 kb on + strand, within BT0161 at 166.814 kb on + strand, within BT0161 at 166.815 kb on - strand, within BT0161 at 166.964 kb on - strand, within BT0161 at 167.147 kb on - strand, within BT0161 at 167.206 kb on + strand, within BT0161 at 167.232 kb on - strand at 167.336 kb on + strand at 167.520 kb on - strand at 167.653 kb on - strand, within BT0162 at 167.722 kb on + strand, within BT0162 at 167.775 kb on - strand, within BT0162 at 168.192 kb on - strand, within BT0162 at 168.300 kb on + strand, within BT0162 at 168.312 kb on - strand, within BT0162 at 168.555 kb on - strand, within BT0162 at 168.582 kb on - strand, within BT0162 at 168.610 kb on - strand, within BT0162 at 168.637 kb on + strand, within BT0162 at 168.810 kb on - strand, within BT0162 at 168.810 kb on - strand, within BT0162 at 169.018 kb on - strand, within BT0162 at 169.182 kb on + strand, within BT0162 at 169.183 kb on - strand, within BT0162 at 169.183 kb on - strand, within BT0162 at 169.310 kb on - strand, within BT0162 at 169.323 kb on - strand, within BT0162 at 169.418 kb on - strand at 169.418 kb on - strand, within BT0162 at 169.573 kb on + strand at 169.576 kb on - strand at 169.607 kb on + strand at 169.669 kb on + strand at 169.670 kb on - strand at 169.675 kb on - strand at 169.693 kb on - strand at 169.768 kb on + strand, within BT0163 at 169.796 kb on - strand, within BT0163 at 169.867 kb on - strand, within BT0163 at 169.898 kb on - strand, within BT0163 at 169.903 kb on + strand, within BT0163 at 169.905 kb on + strand, within BT0163 at 169.986 kb on + strand at 169.986 kb on + strand at 169.988 kb on + strand at 170.006 kb on + strand at 170.053 kb on + strand at 170.130 kb on - strand at 170.130 kb on - strand at 170.180 kb on + strand at 170.180 kb on + strand at 170.563 kb on + strand, within BT0164
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 166,374 + BT0161 0.19 -1.0 166,379 - BT0161 0.19 -2.9 166,508 + BT0161 0.30 +1.6 166,814 + BT0161 0.56 -0.0 166,815 - BT0161 0.57 -0.8 166,964 - BT0161 0.69 -2.8 167,147 - BT0161 0.85 -1.6 167,206 + BT0161 0.90 -3.8 167,232 - -0.6 167,336 + -2.8 167,520 - -0.9 167,653 - BT0162 0.13 -0.7 167,722 + BT0162 0.16 -1.3 167,775 - BT0162 0.19 +1.6 168,192 - BT0162 0.37 -2.8 168,300 + BT0162 0.41 -1.5 168,312 - BT0162 0.42 -0.0 168,555 - BT0162 0.52 -0.2 168,582 - BT0162 0.53 -2.3 168,610 - BT0162 0.55 -0.0 168,637 + BT0162 0.56 -3.7 168,810 - BT0162 0.63 -1.0 168,810 - BT0162 0.63 -2.5 169,018 - BT0162 0.72 -1.0 169,182 + BT0162 0.79 -1.6 169,183 - BT0162 0.79 -1.0 169,183 - BT0162 0.79 -0.0 169,310 - BT0162 0.85 -2.2 169,323 - BT0162 0.85 -0.0 169,418 - -1.0 169,418 - BT0162 0.89 +0.4 169,573 + -1.6 169,576 - -1.6 169,607 + -0.0 169,669 + -1.0 169,670 - -1.3 169,675 - -3.0 169,693 - -1.6 169,768 + BT0163 0.22 -1.6 169,796 - BT0163 0.33 -0.5 169,867 - BT0163 0.59 -1.0 169,898 - BT0163 0.71 -2.3 169,903 + BT0163 0.73 +2.3 169,905 + BT0163 0.73 +1.4 169,986 + -0.0 169,986 + -0.0 169,988 + -1.7 170,006 + -2.5 170,053 + +1.6 170,130 - -0.0 170,130 - -2.6 170,180 + -1.0 170,180 + -1.0 170,563 + BT0164 0.11 -1.6
Or see this region's nucleotide sequence