Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0061 and BT0062 overlap by 4 nucleotides BT0062 and BT0063 are separated by 52 nucleotides BT0063 and BT0064 are separated by 20 nucleotides BT0064 and BT0065 are separated by 69 nucleotides
BT0061: BT0061 - putative tyrosine-protein kinase ptk (NCBI ptt file), at 56,555 to 58,894
BT0061
BT0062: BT0062 - hypothetical protein (NCBI ptt file), at 58,891 to 59,103
BT0062
BT0063: BT0063 - hypothetical protein (NCBI ptt file), at 59,156 to 59,356
BT0063
BT0064: BT0064 - hypothetical protein (NCBI ptt file), at 59,377 to 61,551
BT0064
BT0065: BT0065 - alpha-galactosidase precursor (NCBI ptt file), at 61,621 to 64,158
BT0065
Position (kb) 59
60
61
62 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 58.625 kb on + strand, within BT0061 at 59.155 kb on + strand at 59.285 kb on - strand, within BT0063 at 59.330 kb on - strand, within BT0063 at 59.351 kb on - strand at 59.361 kb on + strand at 59.362 kb on - strand at 59.362 kb on - strand at 59.460 kb on + strand at 59.461 kb on - strand at 59.490 kb on + strand at 59.599 kb on + strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.629 kb on + strand, within BT0064 at 59.668 kb on + strand, within BT0064 at 59.669 kb on - strand, within BT0064 at 59.767 kb on + strand, within BT0064 at 60.175 kb on - strand, within BT0064 at 60.406 kb on + strand, within BT0064 at 60.443 kb on - strand, within BT0064 at 60.646 kb on - strand, within BT0064 at 60.648 kb on + strand, within BT0064 at 60.780 kb on - strand, within BT0064 at 60.784 kb on - strand, within BT0064 at 60.972 kb on - strand, within BT0064 at 60.982 kb on - strand, within BT0064 at 60.982 kb on - strand, within BT0064 at 60.982 kb on - strand, within BT0064 at 60.988 kb on - strand, within BT0064 at 61.016 kb on - strand, within BT0064 at 61.048 kb on - strand, within BT0064 at 61.048 kb on - strand, within BT0064 at 61.095 kb on + strand, within BT0064 at 61.096 kb on - strand, within BT0064 at 61.300 kb on + strand, within BT0064 at 61.300 kb on + strand, within BT0064 at 61.303 kb on + strand, within BT0064 at 61.306 kb on + strand, within BT0064 at 61.332 kb on + strand, within BT0064 at 61.333 kb on - strand, within BT0064 at 61.606 kb on + strand at 61.606 kb on + strand at 61.631 kb on + strand at 61.853 kb on - strand at 61.926 kb on + strand, within BT0065 at 61.954 kb on + strand, within BT0065 at 61.961 kb on - strand, within BT0065 at 61.964 kb on - strand, within BT0065
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 58,625 + BT0061 0.88 -1.6 59,155 + -2.6 59,285 - BT0063 0.64 -3.5 59,330 - BT0063 0.87 -2.4 59,351 - +1.0 59,361 + +0.0 59,362 - -0.7 59,362 - -0.5 59,460 + -0.8 59,461 - -1.8 59,490 + -0.6 59,599 + BT0064 0.10 -2.0 59,624 - BT0064 0.11 +0.0 59,624 - BT0064 0.11 +0.0 59,624 - BT0064 0.11 -1.9 59,624 - BT0064 0.11 -1.0 59,624 - BT0064 0.11 -3.0 59,624 - BT0064 0.11 -0.3 59,629 + BT0064 0.12 -1.3 59,668 + BT0064 0.13 +0.0 59,669 - BT0064 0.13 -1.0 59,767 + BT0064 0.18 +0.4 60,175 - BT0064 0.37 -0.6 60,406 + BT0064 0.47 +0.0 60,443 - BT0064 0.49 +0.6 60,646 - BT0064 0.58 +0.4 60,648 + BT0064 0.58 -1.3 60,780 - BT0064 0.65 -0.6 60,784 - BT0064 0.65 -0.1 60,972 - BT0064 0.73 +0.0 60,982 - BT0064 0.74 +0.0 60,982 - BT0064 0.74 +0.0 60,982 - BT0064 0.74 +0.0 60,988 - BT0064 0.74 -1.6 61,016 - BT0064 0.75 +0.0 61,048 - BT0064 0.77 -0.0 61,048 - BT0064 0.77 -0.4 61,095 + BT0064 0.79 -2.0 61,096 - BT0064 0.79 -1.0 61,300 + BT0064 0.88 -2.6 61,300 + BT0064 0.88 +0.0 61,303 + BT0064 0.89 -3.2 61,306 + BT0064 0.89 -2.9 61,332 + BT0064 0.90 -1.6 61,333 - BT0064 0.90 +0.6 61,606 + +0.0 61,606 + +1.6 61,631 + -0.7 61,853 - +0.0 61,926 + BT0065 0.12 +0.0 61,954 + BT0065 0.13 +0.0 61,961 - BT0065 0.13 -0.8 61,964 - BT0065 0.14 -0.1
Or see this region's nucleotide sequence