Experiment: BHIS with Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt p5482_18 and p5482_19 are separated by 4 nucleotides p5482_19 and p5482_20 are separated by 18 nucleotides p5482_20 and p5482_21 are separated by 556 nucleotides p5482_21 and p5482_22 are separated by 82 nucleotides
p5482_18: p5482_18 - hypothetical protein (NCBI), at 16,898 to 17,605
_18
p5482_19: p5482_19 - hypothetical protein (NCBI), at 17,610 to 18,116
_19
p5482_20: p5482_20 - hypothetical protein (NCBI), at 18,135 to 18,590
_20
p5482_21: p5482_21 - hypothetical protein (NCBI), at 19,147 to 19,419
_21
p5482_22: p5482_22 - hypothetical protein (NCBI), at 19,502 to 19,945
_22
Position (kb) 18
19 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 17.145 kb on - strand, within p5482_18 at 17.231 kb on + strand, within p5482_18 at 17.231 kb on + strand, within p5482_18 at 17.231 kb on + strand, within p5482_18 at 17.231 kb on + strand, within p5482_18 at 17.231 kb on + strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.232 kb on - strand, within p5482_18 at 17.308 kb on - strand, within p5482_18 at 17.308 kb on - strand, within p5482_18 at 17.308 kb on - strand, within p5482_18 at 17.313 kb on + strand, within p5482_18 at 17.313 kb on + strand, within p5482_18 at 17.313 kb on + strand, within p5482_18 at 17.314 kb on - strand, within p5482_18 at 17.314 kb on - strand, within p5482_18 at 17.314 kb on - strand, within p5482_18 at 17.339 kb on - strand, within p5482_18 at 17.339 kb on - strand, within p5482_18 at 17.339 kb on - strand, within p5482_18 at 17.339 kb on - strand, within p5482_18 at 17.367 kb on - strand, within p5482_18 at 17.438 kb on + strand, within p5482_18 at 17.440 kb on + strand, within p5482_18 at 17.441 kb on - strand, within p5482_18 at 17.441 kb on - strand, within p5482_18 at 17.441 kb on - strand, within p5482_18 at 17.441 kb on - strand, within p5482_18 at 17.583 kb on + strand at 17.583 kb on + strand at 17.583 kb on + strand at 17.584 kb on - strand at 17.584 kb on - strand at 17.584 kb on - strand at 17.584 kb on - strand at 17.584 kb on - strand at 17.584 kb on - strand at 17.590 kb on - strand at 17.590 kb on - strand at 17.596 kb on - strand at 17.610 kb on - strand at 17.629 kb on + strand at 17.629 kb on + strand at 17.670 kb on + strand, within p5482_19 at 17.670 kb on + strand, within p5482_19 at 17.671 kb on - strand, within p5482_19 at 17.671 kb on - strand, within p5482_19 at 17.671 kb on - strand, within p5482_19 at 17.671 kb on - strand, within p5482_19 at 17.671 kb on - strand, within p5482_19 at 17.685 kb on + strand, within p5482_19 at 17.685 kb on + strand, within p5482_19 at 17.686 kb on - strand, within p5482_19 at 17.687 kb on + strand, within p5482_19 at 17.687 kb on + strand, within p5482_19 at 17.688 kb on - strand, within p5482_19 at 17.706 kb on + strand, within p5482_19 at 17.707 kb on - strand, within p5482_19 at 17.709 kb on - strand, within p5482_19 at 17.709 kb on - strand, within p5482_19 at 17.757 kb on + strand, within p5482_19 at 17.757 kb on + strand, within p5482_19 at 17.757 kb on + strand, within p5482_19 at 17.757 kb on + strand, within p5482_19 at 17.758 kb on - strand, within p5482_19 at 17.786 kb on + strand, within p5482_19 at 17.787 kb on - strand, within p5482_19 at 17.787 kb on - strand, within p5482_19 at 17.787 kb on - strand, within p5482_19 at 17.787 kb on - strand, within p5482_19 at 17.787 kb on - strand, within p5482_19 at 17.853 kb on + strand, within p5482_19 at 17.854 kb on - strand, within p5482_19 at 17.856 kb on + strand, within p5482_19 at 17.856 kb on + strand, within p5482_19 at 17.856 kb on + strand, within p5482_19 at 17.857 kb on - strand, within p5482_19 at 17.946 kb on + strand, within p5482_19 at 17.947 kb on - strand, within p5482_19 at 17.947 kb on - strand, within p5482_19 at 17.947 kb on - strand at 17.979 kb on + strand, within p5482_19 at 17.979 kb on + strand, within p5482_19 at 17.980 kb on - strand, within p5482_19 at 18.011 kb on - strand, within p5482_19 at 18.059 kb on + strand, within p5482_19 at 18.059 kb on + strand, within p5482_19 at 18.060 kb on - strand, within p5482_19 at 18.077 kb on + strand at 18.077 kb on + strand at 18.081 kb on + strand at 18.109 kb on - strand at 18.112 kb on + strand at 18.113 kb on - strand at 18.113 kb on - strand at 18.115 kb on - strand at 18.121 kb on - strand at 18.124 kb on + strand at 18.134 kb on + strand at 18.135 kb on - strand at 18.144 kb on + strand at 18.145 kb on - strand at 18.145 kb on - strand at 18.150 kb on + strand at 18.151 kb on - strand at 18.151 kb on - strand at 18.237 kb on + strand, within p5482_20 at 18.237 kb on + strand, within p5482_20 at 18.237 kb on + strand, within p5482_20 at 18.237 kb on + strand, within p5482_20 at 18.237 kb on + strand, within p5482_20 at 18.237 kb on + strand, within p5482_20 at 18.238 kb on - strand, within p5482_20 at 18.238 kb on - strand, within p5482_20 at 18.238 kb on - strand, within p5482_20 at 18.238 kb on - strand, within p5482_20 at 18.245 kb on - strand, within p5482_20 at 18.281 kb on + strand, within p5482_20 at 18.281 kb on + strand, within p5482_20 at 18.281 kb on + strand, within p5482_20 at 18.281 kb on + strand, within p5482_20 at 18.282 kb on - strand, within p5482_20 at 18.295 kb on + strand, within p5482_20 at 18.308 kb on + strand, within p5482_20 at 18.362 kb on - strand, within p5482_20 at 18.362 kb on - strand, within p5482_20 at 18.397 kb on + strand, within p5482_20 at 18.556 kb on + strand at 18.556 kb on + strand at 18.556 kb on + strand at 18.556 kb on + strand at 18.557 kb on - strand at 18.557 kb on - strand at 18.557 kb on - strand at 18.560 kb on + strand at 18.560 kb on + strand at 18.560 kb on + strand at 18.572 kb on + strand at 18.579 kb on + strand at 18.582 kb on + strand at 18.583 kb on - strand at 18.583 kb on - strand at 18.583 kb on - strand at 18.584 kb on + strand at 18.585 kb on - strand at 18.585 kb on - strand at 18.672 kb on - strand at 18.672 kb on - strand at 18.706 kb on + strand at 18.707 kb on - strand at 18.707 kb on - strand at 18.841 kb on - strand at 18.847 kb on + strand at 18.847 kb on + strand at 18.875 kb on + strand at 18.875 kb on + strand at 18.875 kb on + strand at 18.876 kb on - strand at 18.876 kb on - strand at 18.876 kb on - strand at 18.879 kb on + strand at 18.879 kb on + strand at 18.879 kb on + strand at 18.880 kb on - strand at 18.928 kb on + strand at 18.929 kb on - strand at 18.941 kb on + strand at 18.942 kb on - strand at 18.968 kb on + strand at 18.969 kb on - strand at 18.977 kb on - strand at 18.977 kb on - strand at 18.977 kb on - strand at 18.990 kb on + strand at 18.990 kb on + strand at 18.991 kb on - strand at 18.995 kb on - strand at 18.995 kb on - strand at 18.995 kb on - strand at 18.995 kb on - strand at 19.043 kb on + strand at 19.044 kb on - strand at 19.086 kb on + strand at 19.087 kb on - strand at 19.087 kb on - strand at 19.087 kb on - strand at 19.087 kb on - strand at 19.091 kb on + strand at 19.095 kb on + strand at 19.104 kb on - strand at 19.124 kb on + strand at 19.124 kb on + strand at 19.127 kb on + strand at 19.128 kb on - strand at 19.136 kb on + strand at 19.136 kb on + strand at 19.146 kb on + strand at 19.158 kb on + strand at 19.158 kb on + strand at 19.159 kb on - strand at 19.159 kb on - strand at 19.222 kb on + strand, within p5482_21 at 19.222 kb on + strand, within p5482_21 at 19.224 kb on + strand, within p5482_21 at 19.224 kb on + strand, within p5482_21 at 19.225 kb on - strand, within p5482_21 at 19.238 kb on + strand, within p5482_21 at 19.342 kb on + strand, within p5482_21 at 19.342 kb on + strand, within p5482_21 at 19.343 kb on - strand, within p5482_21 at 19.356 kb on + strand, within p5482_21 at 19.357 kb on - strand, within p5482_21 at 19.450 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.530 kb on + strand at 19.531 kb on - strand at 19.558 kb on + strand, within p5482_22 at 19.558 kb on + strand, within p5482_22
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.0125 mM remove 17,145 - p5482_18 0.35 -0.1 17,231 + p5482_18 0.47 -2.3 17,231 + p5482_18 0.47 -1.1 17,231 + p5482_18 0.47 -0.2 17,231 + p5482_18 0.47 -0.9 17,231 + p5482_18 0.47 +0.3 17,232 - p5482_18 0.47 +0.3 17,232 - p5482_18 0.47 -0.8 17,232 - p5482_18 0.47 -0.1 17,232 - +0.3 17,232 - p5482_18 0.47 -0.1 17,232 - p5482_18 0.47 +0.3 17,232 - p5482_18 0.47 -1.3 17,232 - p5482_18 0.47 -1.5 17,232 - p5482_18 0.47 -2.2 17,308 - p5482_18 0.58 -0.7 17,308 - p5482_18 0.58 -0.6 17,308 - p5482_18 0.58 -1.0 17,313 + p5482_18 0.59 -2.1 17,313 + p5482_18 0.59 -1.9 17,313 + p5482_18 0.59 -0.9 17,314 - p5482_18 0.59 -1.1 17,314 - p5482_18 0.59 -0.7 17,314 - p5482_18 0.59 -0.0 17,339 - p5482_18 0.62 -2.5 17,339 - p5482_18 0.62 -2.0 17,339 - p5482_18 0.62 -0.2 17,339 - p5482_18 0.62 +1.8 17,367 - p5482_18 0.66 -1.3 17,438 + p5482_18 0.76 -1.2 17,440 + p5482_18 0.77 -2.7 17,441 - p5482_18 0.77 -0.5 17,441 - p5482_18 0.77 -1.2 17,441 - p5482_18 0.77 +3.4 17,441 - p5482_18 0.77 -1.1 17,583 + -2.1 17,583 + +0.2 17,583 + -1.7 17,584 - +0.8 17,584 - -0.4 17,584 - -0.4 17,584 - -0.5 17,584 - -0.8 17,584 - -2.3 17,590 - -2.1 17,590 - +0.3 17,596 - -0.3 17,610 - +0.1 17,629 + -1.6 17,629 + -0.2 17,670 + p5482_19 0.12 -1.2 17,670 + p5482_19 0.12 -1.2 17,671 - p5482_19 0.12 -4.7 17,671 - p5482_19 0.12 -0.9 17,671 - p5482_19 0.12 -0.7 17,671 - p5482_19 0.12 -0.4 17,671 - p5482_19 0.12 -2.4 17,685 + p5482_19 0.15 +0.3 17,685 + p5482_19 0.15 -0.8 17,686 - p5482_19 0.15 -2.7 17,687 + p5482_19 0.15 -0.1 17,687 + p5482_19 0.15 -1.7 17,688 - p5482_19 0.15 -2.3 17,706 + p5482_19 0.19 -2.9 17,707 - p5482_19 0.19 +0.1 17,709 - p5482_19 0.20 -2.1 17,709 - p5482_19 0.20 -0.2 17,757 + p5482_19 0.29 -2.2 17,757 + p5482_19 0.29 -0.3 17,757 + p5482_19 0.29 +0.2 17,757 + p5482_19 0.29 -0.4 17,758 - p5482_19 0.29 -0.1 17,786 + p5482_19 0.35 -1.8 17,787 - p5482_19 0.35 +1.1 17,787 - p5482_19 0.35 +1.5 17,787 - p5482_19 0.35 +1.0 17,787 - p5482_19 0.35 -1.2 17,787 - p5482_19 0.35 -1.3 17,853 + p5482_19 0.48 -0.5 17,854 - p5482_19 0.48 -0.7 17,856 + p5482_19 0.49 +1.6 17,856 + p5482_19 0.49 -1.4 17,856 + p5482_19 0.49 +0.3 17,857 - p5482_19 0.49 -1.0 17,946 + p5482_19 0.66 -0.5 17,947 - p5482_19 0.66 -1.6 17,947 - p5482_19 0.66 -0.2 17,947 - +0.3 17,979 + p5482_19 0.73 -2.4 17,979 + p5482_19 0.73 +3.0 17,980 - p5482_19 0.73 -0.5 18,011 - p5482_19 0.79 -1.0 18,059 + p5482_19 0.89 -0.5 18,059 + p5482_19 0.89 -2.4 18,060 - p5482_19 0.89 -0.9 18,077 + -1.3 18,077 + -1.3 18,081 + -0.7 18,109 - +0.3 18,112 + -1.2 18,113 - -1.7 18,113 - -4.1 18,115 - +0.4 18,121 - -2.4 18,124 + -2.1 18,134 + -0.9 18,135 - -1.1 18,144 + -1.9 18,145 - +0.4 18,145 - -0.2 18,150 + -2.2 18,151 - -0.4 18,151 - -1.5 18,237 + p5482_20 0.22 +0.6 18,237 + p5482_20 0.22 -0.6 18,237 + p5482_20 0.22 -1.1 18,237 + p5482_20 0.22 +1.3 18,237 + p5482_20 0.22 -1.3 18,237 + p5482_20 0.22 -0.6 18,238 - p5482_20 0.23 -1.3 18,238 - p5482_20 0.23 -1.5 18,238 - p5482_20 0.23 -0.6 18,238 - p5482_20 0.23 -1.4 18,245 - p5482_20 0.24 -0.1 18,281 + p5482_20 0.32 -2.2 18,281 + p5482_20 0.32 -1.8 18,281 + p5482_20 0.32 -1.7 18,281 + p5482_20 0.32 -0.9 18,282 - p5482_20 0.32 +2.3 18,295 + p5482_20 0.35 -3.1 18,308 + p5482_20 0.38 -0.9 18,362 - p5482_20 0.50 -2.9 18,362 - p5482_20 0.50 -1.7 18,397 + p5482_20 0.57 +0.3 18,556 + -0.8 18,556 + -2.1 18,556 + -0.7 18,556 + +1.3 18,557 - -2.3 18,557 - -0.4 18,557 - -2.7 18,560 + -2.1 18,560 + -0.3 18,560 + +0.0 18,572 + -1.3 18,579 + +0.3 18,582 + -2.2 18,583 - -1.0 18,583 - -1.4 18,583 - -0.3 18,584 + -0.1 18,585 - -0.9 18,585 - -0.3 18,672 - -1.8 18,672 - -0.5 18,706 + -1.0 18,707 - -1.2 18,707 - -0.0 18,841 - +0.3 18,847 + -3.4 18,847 + -1.3 18,875 + -1.4 18,875 + -0.9 18,875 + -1.2 18,876 - -0.8 18,876 - -1.3 18,876 - -2.7 18,879 + -0.8 18,879 + -0.7 18,879 + -0.7 18,880 - -3.6 18,928 + -4.6 18,929 - -3.9 18,941 + -1.0 18,942 - -1.7 18,968 + -0.7 18,969 - -0.7 18,977 - -3.8 18,977 - -4.1 18,977 - -2.7 18,990 + -4.1 18,990 + -0.6 18,991 - -0.3 18,995 - -4.1 18,995 - -2.3 18,995 - -1.7 18,995 - -1.9 19,043 + +2.6 19,044 - +0.3 19,086 + -0.5 19,087 - -0.3 19,087 - -3.1 19,087 - -1.0 19,087 - -0.3 19,091 + -4.5 19,095 + -1.6 19,104 - -0.5 19,124 + -3.4 19,124 + -1.8 19,127 + -1.7 19,128 - -0.4 19,136 + -1.7 19,136 + -0.5 19,146 + -0.9 19,158 + -3.1 19,158 + -2.6 19,159 - -2.6 19,159 - -2.1 19,222 + p5482_21 0.27 -0.3 19,222 + p5482_21 0.27 -1.7 19,224 + p5482_21 0.28 -3.5 19,224 + p5482_21 0.28 -0.9 19,225 - p5482_21 0.29 +0.3 19,238 + p5482_21 0.33 -1.8 19,342 + p5482_21 0.71 -0.7 19,342 + p5482_21 0.71 -1.1 19,343 - p5482_21 0.72 -1.5 19,356 + p5482_21 0.77 -0.7 19,357 - p5482_21 0.77 -3.2 19,450 + -1.3 19,530 + -2.6 19,530 + -1.7 19,530 + -0.0 19,530 + -2.6 19,530 + -0.3 19,531 - -0.7 19,558 + p5482_22 0.13 -1.4 19,558 + p5482_22 0.13 -2.1
Or see this region's nucleotide sequence