Experiment: MoLS4 with L-Alanine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1893 and DVU1894 are separated by 210 nucleotides DVU1894 and DVU1895 are separated by 231 nucleotides DVU1895 and rpsT are separated by 565 nucleotides rpsT and glyS are separated by 115 nucleotides
DVU1893: DVU1893 - ATP-dependent protease, putative (TIGR), at 1,965,307 to 1,967,727
DVU1893
DVU1894: DVU1894 - hypothetical protein (TIGR), at 1,967,938 to 1,968,132
DVU1894
DVU1895: DVU1895 - major facilitator superfamily protein (TIGR), at 1,968,364 to 1,969,530
DVU1895
DVU1896: rpsT - ribosomal protein S20 (TIGR), at 1,970,096 to 1,970,359
rpsT
DVU1897: glyS - glycyl-tRNA synthetase, beta subunit (TIGR), at 1,970,475 to 1,972,565
glyS
Position (kb)
1968
1969
1970 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1967.399 kb on - strand, within DVU1893 at 1967.490 kb on + strand at 1967.498 kb on - strand at 1967.539 kb on + strand at 1967.593 kb on - strand at 1967.593 kb on - strand at 1967.765 kb on - strand at 1967.991 kb on + strand, within DVU1894 at 1968.011 kb on - strand, within DVU1894 at 1968.043 kb on + strand, within DVU1894 at 1968.084 kb on + strand, within DVU1894 at 1968.156 kb on + strand at 1968.156 kb on + strand at 1968.156 kb on + strand at 1968.164 kb on - strand at 1968.164 kb on - strand at 1968.216 kb on + strand at 1968.224 kb on - strand at 1968.229 kb on + strand at 1968.242 kb on - strand at 1968.258 kb on + strand at 1968.270 kb on + strand at 1968.270 kb on + strand at 1968.333 kb on - strand at 1968.363 kb on - strand at 1968.390 kb on - strand at 1968.493 kb on - strand, within DVU1895 at 1968.606 kb on - strand, within DVU1895 at 1968.720 kb on + strand, within DVU1895 at 1968.790 kb on + strand, within DVU1895 at 1968.903 kb on - strand, within DVU1895 at 1969.141 kb on + strand, within DVU1895 at 1969.141 kb on + strand, within DVU1895 at 1969.159 kb on - strand, within DVU1895 at 1969.231 kb on - strand, within DVU1895 at 1969.378 kb on - strand, within DVU1895 at 1969.390 kb on + strand, within DVU1895 at 1969.390 kb on + strand, within DVU1895 at 1969.462 kb on + strand at 1969.470 kb on - strand at 1969.539 kb on + strand at 1969.545 kb on + strand at 1969.545 kb on - strand at 1969.701 kb on + strand at 1969.895 kb on - strand at 1969.962 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Alanine as nutrient 10mM remove 1,967,399 - DVU1893 0.86 -0.6 1,967,490 + -0.6 1,967,498 - -0.6 1,967,539 + -0.9 1,967,593 - +0.1 1,967,593 - +3.5 1,967,765 - -0.7 1,967,991 + DVU1894 0.27 -1.3 1,968,011 - DVU1894 0.37 -0.1 1,968,043 + DVU1894 0.54 +0.1 1,968,084 + DVU1894 0.75 -0.1 1,968,156 + +0.0 1,968,156 + -1.2 1,968,156 + +0.2 1,968,164 - -0.9 1,968,164 - -0.2 1,968,216 + +0.2 1,968,224 - +0.1 1,968,229 + +0.1 1,968,242 - -0.0 1,968,258 + +0.5 1,968,270 + -0.3 1,968,270 + -0.0 1,968,333 - -0.1 1,968,363 - +0.4 1,968,390 - +0.0 1,968,493 - DVU1895 0.11 -1.5 1,968,606 - DVU1895 0.21 -1.8 1,968,720 + DVU1895 0.31 -1.1 1,968,790 + DVU1895 0.37 +0.6 1,968,903 - DVU1895 0.46 -0.2 1,969,141 + DVU1895 0.67 -0.4 1,969,141 + DVU1895 0.67 -1.4 1,969,159 - DVU1895 0.68 -1.1 1,969,231 - DVU1895 0.74 -1.2 1,969,378 - DVU1895 0.87 -1.6 1,969,390 + DVU1895 0.88 -0.1 1,969,390 + DVU1895 0.88 -0.6 1,969,462 + -1.3 1,969,470 - -1.6 1,969,539 + +0.1 1,969,545 + -0.7 1,969,545 - -0.1 1,969,701 + -0.4 1,969,895 - -0.1 1,969,962 - +0.1
Or see this region's nucleotide sequence