Experiment: 9:9 LD Cycle Bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1990 and mscL are separated by 392 nucleotides mscL and eIF2BA are separated by 66 nucleotides
Synpcc7942_1990: Synpcc7942_1990 - hypothetical protein, at 2,060,865 to 2,061,527
_1990
Synpcc7942_1991: mscL - large conductance mechanosensitive channel protein, at 2,061,920 to 2,062,321
mscL
Synpcc7942_1992: eIF2BA - translation initiation factor 2B subunit I family (IF-2BI), at 2,062,388 to 2,063,434
eIF2BA
Position (kb)
2061
2062
2063 Strain fitness (log2 ratio)
-2
-1
0
1 at 2060.931 kb on + strand at 2060.947 kb on + strand, within Synpcc7942_1990 at 2060.990 kb on - strand, within Synpcc7942_1990 at 2061.023 kb on + strand, within Synpcc7942_1990 at 2061.039 kb on - strand, within Synpcc7942_1990 at 2061.163 kb on - strand, within Synpcc7942_1990 at 2061.205 kb on - strand, within Synpcc7942_1990 at 2061.355 kb on + strand, within Synpcc7942_1990 at 2061.360 kb on + strand, within Synpcc7942_1990 at 2061.360 kb on + strand, within Synpcc7942_1990 at 2061.368 kb on - strand, within Synpcc7942_1990 at 2061.370 kb on + strand, within Synpcc7942_1990 at 2061.378 kb on - strand, within Synpcc7942_1990 at 2061.474 kb on + strand at 2061.474 kb on + strand at 2061.482 kb on - strand at 2061.525 kb on - strand at 2061.917 kb on + strand at 2061.927 kb on + strand at 2061.936 kb on - strand at 2062.084 kb on - strand, within mscL at 2062.158 kb on + strand, within mscL at 2062.203 kb on + strand, within mscL at 2062.211 kb on - strand, within mscL at 2062.347 kb on - strand at 2062.358 kb on + strand at 2062.364 kb on - strand at 2062.365 kb on - strand at 2062.384 kb on - strand at 2062.390 kb on + strand at 2062.393 kb on - strand at 2062.408 kb on - strand at 2062.424 kb on + strand at 2062.424 kb on + strand at 2062.424 kb on + strand at 2062.434 kb on + strand at 2062.450 kb on + strand at 2062.477 kb on - strand at 2062.618 kb on + strand, within eIF2BA at 2062.629 kb on - strand, within eIF2BA at 2062.643 kb on - strand, within eIF2BA at 2062.699 kb on + strand, within eIF2BA at 2062.710 kb on - strand, within eIF2BA at 2062.743 kb on + strand, within eIF2BA at 2062.805 kb on - strand, within eIF2BA at 2062.807 kb on - strand, within eIF2BA at 2062.809 kb on + strand, within eIF2BA at 2062.817 kb on - strand, within eIF2BA at 2062.829 kb on + strand, within eIF2BA at 2062.853 kb on - strand at 2062.901 kb on - strand, within eIF2BA at 2062.918 kb on - strand, within eIF2BA at 2062.919 kb on + strand, within eIF2BA at 2062.927 kb on - strand, within eIF2BA at 2062.927 kb on - strand, within eIF2BA at 2062.927 kb on - strand, within eIF2BA at 2062.934 kb on + strand, within eIF2BA at 2062.941 kb on + strand, within eIF2BA at 2062.967 kb on - strand, within eIF2BA at 2062.969 kb on + strand, within eIF2BA at 2063.012 kb on - strand, within eIF2BA at 2063.046 kb on + strand at 2063.051 kb on + strand, within eIF2BA at 2063.087 kb on - strand, within eIF2BA at 2063.215 kb on + strand, within eIF2BA at 2063.215 kb on + strand, within eIF2BA at 2063.237 kb on + strand, within eIF2BA at 2063.246 kb on + strand, within eIF2BA at 2063.266 kb on - strand, within eIF2BA at 2063.301 kb on + strand, within eIF2BA at 2063.306 kb on + strand, within eIF2BA at 2063.306 kb on + strand, within eIF2BA at 2063.314 kb on - strand, within eIF2BA
Per-strain Table
Position Strand Gene LocusTag Fraction 9:9 LD Cycle Bioreactor remove 2,060,931 + -1.9 2,060,947 + Synpcc7942_1990 0.12 -1.0 2,060,990 - Synpcc7942_1990 0.19 +1.6 2,061,023 + Synpcc7942_1990 0.24 +1.6 2,061,039 - Synpcc7942_1990 0.26 +1.6 2,061,163 - Synpcc7942_1990 0.45 +0.3 2,061,205 - Synpcc7942_1990 0.51 -0.2 2,061,355 + Synpcc7942_1990 0.74 -0.4 2,061,360 + Synpcc7942_1990 0.75 +1.6 2,061,360 + Synpcc7942_1990 0.75 -0.0 2,061,368 - Synpcc7942_1990 0.76 +0.1 2,061,370 + Synpcc7942_1990 0.76 +0.6 2,061,378 - Synpcc7942_1990 0.77 +1.6 2,061,474 + +0.6 2,061,474 + -1.2 2,061,482 - +1.6 2,061,525 - +1.6 2,061,917 + -1.0 2,061,927 + +0.7 2,061,936 - +0.3 2,062,084 - mscL Synpcc7942_1991 0.41 -0.0 2,062,158 + mscL Synpcc7942_1991 0.59 +0.4 2,062,203 + mscL Synpcc7942_1991 0.70 +0.0 2,062,211 - mscL Synpcc7942_1991 0.72 -0.3 2,062,347 - +0.1 2,062,358 + -0.2 2,062,364 - -0.8 2,062,365 - +0.8 2,062,384 - +0.6 2,062,390 + +1.6 2,062,393 - -0.5 2,062,408 - -1.1 2,062,424 + +0.3 2,062,424 + +0.1 2,062,424 + -1.2 2,062,434 + +1.4 2,062,450 + -0.3 2,062,477 - +0.2 2,062,618 + eIF2BA Synpcc7942_1992 0.22 -1.0 2,062,629 - eIF2BA Synpcc7942_1992 0.23 +1.6 2,062,643 - eIF2BA Synpcc7942_1992 0.24 -0.4 2,062,699 + eIF2BA Synpcc7942_1992 0.30 -0.6 2,062,710 - eIF2BA Synpcc7942_1992 0.31 -0.9 2,062,743 + eIF2BA Synpcc7942_1992 0.34 +0.2 2,062,805 - eIF2BA Synpcc7942_1992 0.40 -0.4 2,062,807 - eIF2BA Synpcc7942_1992 0.40 -0.4 2,062,809 + eIF2BA Synpcc7942_1992 0.40 -1.4 2,062,817 - eIF2BA Synpcc7942_1992 0.41 -1.9 2,062,829 + eIF2BA Synpcc7942_1992 0.42 +0.8 2,062,853 - -0.3 2,062,901 - eIF2BA Synpcc7942_1992 0.49 -0.1 2,062,918 - eIF2BA Synpcc7942_1992 0.51 +0.4 2,062,919 + eIF2BA Synpcc7942_1992 0.51 -0.4 2,062,927 - eIF2BA Synpcc7942_1992 0.51 -0.4 2,062,927 - eIF2BA Synpcc7942_1992 0.51 -0.0 2,062,927 - eIF2BA Synpcc7942_1992 0.51 +1.6 2,062,934 + eIF2BA Synpcc7942_1992 0.52 +1.0 2,062,941 + eIF2BA Synpcc7942_1992 0.53 -1.0 2,062,967 - eIF2BA Synpcc7942_1992 0.55 -0.7 2,062,969 + eIF2BA Synpcc7942_1992 0.55 +0.6 2,063,012 - eIF2BA Synpcc7942_1992 0.60 +0.1 2,063,046 + -0.0 2,063,051 + eIF2BA Synpcc7942_1992 0.63 +0.9 2,063,087 - eIF2BA Synpcc7942_1992 0.67 -0.0 2,063,215 + eIF2BA Synpcc7942_1992 0.79 -0.5 2,063,215 + eIF2BA Synpcc7942_1992 0.79 +0.7 2,063,237 + eIF2BA Synpcc7942_1992 0.81 -1.0 2,063,246 + eIF2BA Synpcc7942_1992 0.82 -2.7 2,063,266 - eIF2BA Synpcc7942_1992 0.84 +0.6 2,063,301 + eIF2BA Synpcc7942_1992 0.87 +0.8 2,063,306 + eIF2BA Synpcc7942_1992 0.88 -0.2 2,063,306 + eIF2BA Synpcc7942_1992 0.88 -1.6 2,063,314 - eIF2BA Synpcc7942_1992 0.88 +0.4
Or see this region's nucleotide sequence