Experiment: 9:9 LD Cycle Bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1862 and hemG are separated by 12 nucleotides hemG and Synpcc7942_1864 are separated by 5 nucleotides Synpcc7942_1864 and ppnK are separated by 296 nucleotides
Synpcc7942_1862: Synpcc7942_1862 - hypothetical protein, at 1,932,765 to 1,933,688
_1862
Synpcc7942_1863: hemG - modification methylase, HemK family, at 1,933,701 to 1,934,582
hemG
Synpcc7942_1864: Synpcc7942_1864 - hypothetical protein, at 1,934,588 to 1,935,175
_1864
Synpcc7942_1865: ppnK - inorganic polyphosphate/ATP-NAD kinase, at 1,935,472 to 1,936,392
ppnK
Position (kb)
1933
1934
1935 Strain fitness (log2 ratio)
-2
-1
0
1 at 1932.707 kb on + strand at 1932.707 kb on + strand at 1932.715 kb on - strand at 1932.719 kb on + strand at 1932.726 kb on + strand at 1932.727 kb on - strand at 1932.817 kb on - strand at 1932.835 kb on - strand at 1932.892 kb on - strand, within Synpcc7942_1862 at 1932.902 kb on - strand, within Synpcc7942_1862 at 1932.927 kb on - strand, within Synpcc7942_1862 at 1933.126 kb on + strand, within Synpcc7942_1862 at 1933.141 kb on - strand, within Synpcc7942_1862 at 1933.402 kb on - strand, within Synpcc7942_1862 at 1933.449 kb on + strand, within Synpcc7942_1862 at 1933.487 kb on + strand, within Synpcc7942_1862 at 1933.565 kb on + strand, within Synpcc7942_1862 at 1933.657 kb on + strand at 1933.660 kb on - strand at 1933.663 kb on + strand at 1933.663 kb on + strand at 1933.663 kb on + strand at 1933.665 kb on - strand at 1933.671 kb on - strand at 1933.738 kb on - strand at 1933.779 kb on + strand at 1933.801 kb on - strand, within hemG at 1933.824 kb on + strand, within hemG at 1933.837 kb on - strand, within hemG at 1933.842 kb on - strand, within hemG at 1933.852 kb on + strand, within hemG at 1933.857 kb on + strand, within hemG at 1933.857 kb on + strand, within hemG at 1933.862 kb on + strand, within hemG at 1933.870 kb on - strand, within hemG at 1933.928 kb on + strand, within hemG at 1933.928 kb on + strand, within hemG at 1933.928 kb on + strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.947 kb on - strand, within hemG at 1933.991 kb on - strand, within hemG at 1934.039 kb on - strand, within hemG at 1934.044 kb on - strand, within hemG at 1934.076 kb on + strand, within hemG at 1934.076 kb on + strand, within hemG at 1934.076 kb on + strand, within hemG at 1934.084 kb on - strand, within hemG at 1934.084 kb on - strand, within hemG at 1934.146 kb on + strand, within hemG at 1934.178 kb on + strand, within hemG at 1934.183 kb on + strand, within hemG at 1934.191 kb on - strand, within hemG at 1934.225 kb on + strand, within hemG at 1934.226 kb on + strand, within hemG at 1934.229 kb on - strand, within hemG at 1934.234 kb on + strand, within hemG at 1934.240 kb on + strand, within hemG at 1934.260 kb on + strand, within hemG at 1934.330 kb on - strand, within hemG at 1934.344 kb on + strand, within hemG at 1934.344 kb on + strand, within hemG at 1934.344 kb on + strand, within hemG at 1934.352 kb on - strand, within hemG at 1934.352 kb on - strand, within hemG at 1934.362 kb on - strand, within hemG at 1934.374 kb on + strand, within hemG at 1934.391 kb on + strand, within hemG at 1934.440 kb on + strand, within hemG at 1934.440 kb on + strand, within hemG at 1934.440 kb on + strand, within hemG at 1934.465 kb on - strand, within hemG at 1934.520 kb on - strand at 1934.529 kb on - strand at 1934.567 kb on + strand at 1934.577 kb on + strand at 1934.577 kb on + strand at 1935.148 kb on + strand at 1935.148 kb on + strand at 1935.164 kb on + strand at 1935.169 kb on + strand at 1935.177 kb on - strand at 1935.190 kb on + strand at 1935.198 kb on - strand at 1935.200 kb on + strand at 1935.203 kb on - strand at 1935.209 kb on - strand at 1935.212 kb on + strand at 1935.212 kb on + strand at 1935.220 kb on - strand at 1935.220 kb on - strand at 1935.293 kb on + strand at 1935.300 kb on - strand at 1935.313 kb on - strand at 1935.320 kb on + strand at 1935.332 kb on + strand at 1935.351 kb on + strand at 1935.359 kb on - strand at 1935.369 kb on + strand at 1935.386 kb on + strand at 1935.394 kb on - strand at 1935.394 kb on - strand at 1935.394 kb on - strand at 1935.394 kb on - strand at 1935.432 kb on + strand at 1935.440 kb on - strand at 1935.444 kb on + strand at 1935.444 kb on + strand at 1935.444 kb on + strand at 1935.446 kb on - strand at 1935.446 kb on - strand at 1935.452 kb on - strand at 1935.452 kb on - strand at 1935.452 kb on - strand at 1935.452 kb on - strand at 1935.480 kb on - strand at 1935.489 kb on + strand at 1935.497 kb on + strand at 1935.578 kb on + strand, within ppnK at 1935.578 kb on + strand, within ppnK at 1935.578 kb on + strand, within ppnK
Per-strain Table
Position Strand Gene LocusTag Fraction 9:9 LD Cycle Bioreactor remove 1,932,707 + -1.6 1,932,707 + -0.0 1,932,715 - -0.0 1,932,719 + -1.4 1,932,726 + -0.1 1,932,727 - +0.8 1,932,817 - +1.6 1,932,835 - +0.6 1,932,892 - Synpcc7942_1862 0.14 -0.4 1,932,902 - Synpcc7942_1862 0.15 -0.0 1,932,927 - Synpcc7942_1862 0.18 +1.6 1,933,126 + Synpcc7942_1862 0.39 +0.6 1,933,141 - Synpcc7942_1862 0.41 -1.6 1,933,402 - Synpcc7942_1862 0.69 -0.0 1,933,449 + Synpcc7942_1862 0.74 -0.4 1,933,487 + Synpcc7942_1862 0.78 +1.6 1,933,565 + Synpcc7942_1862 0.87 +0.6 1,933,657 + +0.1 1,933,660 - -0.7 1,933,663 + -0.8 1,933,663 + +0.2 1,933,663 + -1.9 1,933,665 - -0.7 1,933,671 - -1.4 1,933,738 - -0.8 1,933,779 + +1.6 1,933,801 - hemG Synpcc7942_1863 0.11 -1.9 1,933,824 + hemG Synpcc7942_1863 0.14 -0.4 1,933,837 - hemG Synpcc7942_1863 0.15 -0.0 1,933,842 - hemG Synpcc7942_1863 0.16 +1.6 1,933,852 + hemG Synpcc7942_1863 0.17 +1.0 1,933,857 + hemG Synpcc7942_1863 0.18 -2.0 1,933,857 + hemG Synpcc7942_1863 0.18 -0.4 1,933,862 + hemG Synpcc7942_1863 0.18 -0.4 1,933,870 - hemG Synpcc7942_1863 0.19 -0.2 1,933,928 + hemG Synpcc7942_1863 0.26 -1.2 1,933,928 + hemG Synpcc7942_1863 0.26 +0.4 1,933,928 + hemG Synpcc7942_1863 0.26 +1.6 1,933,936 - hemG Synpcc7942_1863 0.27 +1.6 1,933,936 - hemG Synpcc7942_1863 0.27 -0.0 1,933,936 - hemG Synpcc7942_1863 0.27 -0.0 1,933,936 - hemG Synpcc7942_1863 0.27 +0.6 1,933,947 - hemG Synpcc7942_1863 0.28 -1.6 1,933,991 - hemG Synpcc7942_1863 0.33 +0.8 1,934,039 - hemG Synpcc7942_1863 0.38 -0.0 1,934,044 - hemG Synpcc7942_1863 0.39 -0.4 1,934,076 + hemG Synpcc7942_1863 0.43 -0.7 1,934,076 + hemG Synpcc7942_1863 0.43 +0.6 1,934,076 + hemG Synpcc7942_1863 0.43 -1.0 1,934,084 - hemG Synpcc7942_1863 0.43 +0.6 1,934,084 - hemG Synpcc7942_1863 0.43 -0.4 1,934,146 + hemG Synpcc7942_1863 0.50 -1.2 1,934,178 + hemG Synpcc7942_1863 0.54 +1.6 1,934,183 + hemG Synpcc7942_1863 0.55 -0.2 1,934,191 - hemG Synpcc7942_1863 0.56 -0.4 1,934,225 + hemG Synpcc7942_1863 0.59 -0.4 1,934,226 + hemG Synpcc7942_1863 0.60 +0.6 1,934,229 - hemG Synpcc7942_1863 0.60 -0.0 1,934,234 + hemG Synpcc7942_1863 0.60 -0.7 1,934,240 + hemG Synpcc7942_1863 0.61 -0.7 1,934,260 + hemG Synpcc7942_1863 0.63 +0.6 1,934,330 - hemG Synpcc7942_1863 0.71 -0.2 1,934,344 + hemG Synpcc7942_1863 0.73 -0.1 1,934,344 + hemG Synpcc7942_1863 0.73 +1.6 1,934,344 + hemG Synpcc7942_1863 0.73 -0.4 1,934,352 - hemG Synpcc7942_1863 0.74 -0.4 1,934,352 - hemG Synpcc7942_1863 0.74 -0.0 1,934,362 - hemG Synpcc7942_1863 0.75 +0.3 1,934,374 + hemG Synpcc7942_1863 0.76 -1.6 1,934,391 + hemG Synpcc7942_1863 0.78 -0.4 1,934,440 + hemG Synpcc7942_1863 0.84 -0.0 1,934,440 + hemG Synpcc7942_1863 0.84 +1.6 1,934,440 + hemG Synpcc7942_1863 0.84 -0.4 1,934,465 - hemG Synpcc7942_1863 0.87 +0.6 1,934,520 - +0.6 1,934,529 - -0.0 1,934,567 + +0.2 1,934,577 + -2.3 1,934,577 + +0.7 1,935,148 + -0.6 1,935,148 + -0.7 1,935,164 + -0.9 1,935,169 + +0.4 1,935,177 - -0.9 1,935,190 + +0.6 1,935,198 - -0.3 1,935,200 + +0.4 1,935,203 - -0.6 1,935,209 - +0.6 1,935,212 + -0.0 1,935,212 + +0.3 1,935,220 - -0.7 1,935,220 - -1.7 1,935,293 + +0.1 1,935,300 - -0.7 1,935,313 - -1.0 1,935,320 + -0.7 1,935,332 + +0.4 1,935,351 + +0.3 1,935,359 - -0.0 1,935,369 + +1.0 1,935,386 + +0.6 1,935,394 - +0.0 1,935,394 - +0.3 1,935,394 - -0.0 1,935,394 - -2.4 1,935,432 + -1.3 1,935,440 - +0.1 1,935,444 + -0.1 1,935,444 + -0.5 1,935,444 + -0.2 1,935,446 - -0.0 1,935,446 - -0.1 1,935,452 - +0.4 1,935,452 - -0.3 1,935,452 - +1.2 1,935,452 - -0.4 1,935,480 - -0.2 1,935,489 + +0.0 1,935,497 + +0.4 1,935,578 + ppnK Synpcc7942_1865 0.12 +0.2 1,935,578 + ppnK Synpcc7942_1865 0.12 +0.7 1,935,578 + ppnK Synpcc7942_1865 0.12 +0.2
Or see this region's nucleotide sequence