Experiment: 9:9 LD Cycle Bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1821 and Synpcc7942_1822 are separated by 27 nucleotides Synpcc7942_1822 and nblB overlap by 4 nucleotides nblB and Synpcc7942_1824 are separated by 6 nucleotides Synpcc7942_1824 and PgsA are separated by 76 nucleotides PgsA and Synpcc7942_1826 overlap by 4 nucleotides
Synpcc7942_1821: Synpcc7942_1821 - hypothetical protein, at 1,893,904 to 1,894,263
_1821
Synpcc7942_1822: Synpcc7942_1822 - hypothetical protein, at 1,894,291 to 1,894,503
_1822
Synpcc7942_1823: nblB - PBS lyase HEAT-like repeat, at 1,894,500 to 1,895,159
nblB
Synpcc7942_1824: Synpcc7942_1824 - CBS, at 1,895,166 to 1,895,630
_1824
Synpcc7942_1825: PgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 1,895,707 to 1,896,225
PgsA
Synpcc7942_1826: Synpcc7942_1826 - mRNA-binding protein, at 1,896,222 to 1,897,163
_1826
Position (kb)
1895
1896 Strain fitness (log2 ratio)
-2
-1
0
1 at 1894.177 kb on + strand, within Synpcc7942_1821 at 1894.187 kb on + strand, within Synpcc7942_1821 at 1894.190 kb on - strand, within Synpcc7942_1821 at 1894.208 kb on - strand, within Synpcc7942_1821 at 1894.229 kb on + strand at 1894.229 kb on + strand at 1894.229 kb on + strand at 1894.229 kb on + strand at 1894.235 kb on - strand at 1894.257 kb on + strand at 1894.266 kb on - strand at 1894.284 kb on - strand at 1894.326 kb on + strand, within Synpcc7942_1822 at 1894.369 kb on + strand, within Synpcc7942_1822 at 1894.378 kb on + strand, within Synpcc7942_1822 at 1894.380 kb on - strand, within Synpcc7942_1822 at 1894.403 kb on - strand, within Synpcc7942_1822 at 1894.497 kb on + strand at 1894.513 kb on - strand at 1894.521 kb on - strand at 1894.534 kb on + strand at 1894.541 kb on - strand at 1894.592 kb on + strand, within nblB at 1894.592 kb on + strand, within nblB at 1894.602 kb on + strand, within nblB at 1894.662 kb on + strand, within nblB at 1894.662 kb on - strand, within nblB at 1894.690 kb on + strand, within nblB at 1894.695 kb on + strand, within nblB at 1894.703 kb on - strand, within nblB at 1894.717 kb on + strand, within nblB at 1894.760 kb on + strand, within nblB at 1894.779 kb on + strand at 1894.787 kb on + strand, within nblB at 1894.804 kb on + strand, within nblB at 1894.812 kb on - strand, within nblB at 1894.812 kb on - strand, within nblB at 1894.858 kb on + strand, within nblB at 1894.858 kb on + strand, within nblB at 1894.883 kb on + strand, within nblB at 1894.883 kb on + strand, within nblB at 1894.913 kb on - strand, within nblB at 1894.949 kb on + strand, within nblB at 1894.959 kb on + strand, within nblB at 1894.993 kb on - strand, within nblB at 1895.014 kb on - strand, within nblB at 1895.120 kb on - strand at 1895.127 kb on + strand at 1895.128 kb on - strand at 1895.171 kb on - strand at 1895.190 kb on + strand at 1895.190 kb on + strand at 1895.192 kb on + strand at 1895.208 kb on - strand at 1895.230 kb on + strand at 1895.230 kb on + strand, within Synpcc7942_1824 at 1895.230 kb on + strand, within Synpcc7942_1824 at 1895.230 kb on + strand, within Synpcc7942_1824 at 1895.230 kb on + strand, within Synpcc7942_1824 at 1895.230 kb on + strand, within Synpcc7942_1824 at 1895.238 kb on - strand, within Synpcc7942_1824 at 1895.238 kb on - strand, within Synpcc7942_1824 at 1895.238 kb on - strand, within Synpcc7942_1824 at 1895.321 kb on - strand, within Synpcc7942_1824 at 1895.321 kb on - strand at 1895.329 kb on + strand, within Synpcc7942_1824 at 1895.329 kb on + strand, within Synpcc7942_1824 at 1895.337 kb on - strand, within Synpcc7942_1824 at 1895.347 kb on + strand, within Synpcc7942_1824 at 1895.352 kb on + strand, within Synpcc7942_1824 at 1895.377 kb on - strand, within Synpcc7942_1824 at 1895.402 kb on + strand, within Synpcc7942_1824 at 1895.402 kb on + strand, within Synpcc7942_1824 at 1895.420 kb on + strand, within Synpcc7942_1824 at 1895.420 kb on + strand, within Synpcc7942_1824 at 1895.444 kb on + strand, within Synpcc7942_1824 at 1895.457 kb on - strand, within Synpcc7942_1824 at 1895.458 kb on + strand, within Synpcc7942_1824 at 1895.463 kb on + strand, within Synpcc7942_1824 at 1895.479 kb on + strand, within Synpcc7942_1824 at 1895.493 kb on - strand, within Synpcc7942_1824 at 1895.501 kb on - strand, within Synpcc7942_1824 at 1895.511 kb on - strand, within Synpcc7942_1824 at 1895.522 kb on + strand, within Synpcc7942_1824 at 1895.524 kb on + strand, within Synpcc7942_1824 at 1895.524 kb on + strand, within Synpcc7942_1824 at 1895.529 kb on + strand, within Synpcc7942_1824 at 1895.535 kb on - strand, within Synpcc7942_1824 at 1895.571 kb on + strand, within Synpcc7942_1824 at 1895.572 kb on + strand, within Synpcc7942_1824 at 1895.572 kb on + strand, within Synpcc7942_1824 at 1895.574 kb on - strand, within Synpcc7942_1824 at 1895.580 kb on - strand, within Synpcc7942_1824 at 1895.595 kb on - strand at 1895.597 kb on - strand at 1895.597 kb on - strand at 1895.602 kb on - strand at 1895.626 kb on + strand at 1895.634 kb on - strand at 1895.637 kb on + strand at 1895.637 kb on + strand at 1895.638 kb on + strand at 1895.638 kb on + strand at 1895.646 kb on - strand at 1895.647 kb on - strand at 1895.647 kb on - strand at 1895.649 kb on + strand at 1895.718 kb on - strand at 1895.722 kb on - strand at 1895.813 kb on + strand, within PgsA at 1896.215 kb on + strand at 1896.229 kb on + strand at 1896.237 kb on - strand at 1896.248 kb on + strand at 1896.260 kb on - strand at 1896.284 kb on + strand at 1896.284 kb on + strand at 1896.317 kb on - strand, within Synpcc7942_1826 at 1896.368 kb on - strand, within Synpcc7942_1826 at 1896.399 kb on - strand, within Synpcc7942_1826 at 1896.421 kb on + strand, within Synpcc7942_1826 at 1896.421 kb on + strand, within Synpcc7942_1826 at 1896.421 kb on + strand, within Synpcc7942_1826 at 1896.421 kb on + strand, within Synpcc7942_1826 at 1896.439 kb on - strand, within Synpcc7942_1826 at 1896.446 kb on + strand, within Synpcc7942_1826 at 1896.446 kb on + strand, within Synpcc7942_1826 at 1896.454 kb on - strand, within Synpcc7942_1826 at 1896.476 kb on - strand, within Synpcc7942_1826 at 1896.497 kb on + strand, within Synpcc7942_1826 at 1896.507 kb on + strand at 1896.507 kb on + strand, within Synpcc7942_1826 at 1896.507 kb on + strand, within Synpcc7942_1826 at 1896.507 kb on + strand, within Synpcc7942_1826 at 1896.529 kb on - strand, within Synpcc7942_1826 at 1896.552 kb on + strand, within Synpcc7942_1826 at 1896.559 kb on - strand, within Synpcc7942_1826 at 1896.561 kb on - strand, within Synpcc7942_1826 at 1896.561 kb on - strand, within Synpcc7942_1826 at 1896.572 kb on + strand, within Synpcc7942_1826 at 1896.594 kb on + strand, within Synpcc7942_1826 at 1896.612 kb on + strand at 1896.612 kb on + strand, within Synpcc7942_1826 at 1896.620 kb on - strand, within Synpcc7942_1826 at 1896.627 kb on + strand, within Synpcc7942_1826
Per-strain Table
Position Strand Gene LocusTag Fraction 9:9 LD Cycle Bioreactor remove 1,894,177 + Synpcc7942_1821 0.76 +0.4 1,894,187 + Synpcc7942_1821 0.79 +0.6 1,894,190 - Synpcc7942_1821 0.79 -1.4 1,894,208 - Synpcc7942_1821 0.84 -1.4 1,894,229 + -0.6 1,894,229 + +1.6 1,894,229 + +0.9 1,894,229 + +0.8 1,894,235 - -1.0 1,894,257 + +0.7 1,894,266 - +1.6 1,894,284 - +0.6 1,894,326 + Synpcc7942_1822 0.16 -0.0 1,894,369 + Synpcc7942_1822 0.37 +1.6 1,894,378 + Synpcc7942_1822 0.41 -0.4 1,894,380 - Synpcc7942_1822 0.42 -0.7 1,894,403 - Synpcc7942_1822 0.53 -1.0 1,894,497 + -0.7 1,894,513 - -0.1 1,894,521 - -0.1 1,894,534 + +0.5 1,894,541 - +0.3 1,894,592 + nblB Synpcc7942_1823 0.14 +0.3 1,894,592 + nblB Synpcc7942_1823 0.14 -1.2 1,894,602 + nblB Synpcc7942_1823 0.15 +0.8 1,894,662 + nblB Synpcc7942_1823 0.25 -0.6 1,894,662 - nblB Synpcc7942_1823 0.25 +1.3 1,894,690 + nblB Synpcc7942_1823 0.29 -0.3 1,894,695 + nblB Synpcc7942_1823 0.30 +0.1 1,894,703 - nblB Synpcc7942_1823 0.31 +1.0 1,894,717 + nblB Synpcc7942_1823 0.33 -1.4 1,894,760 + nblB Synpcc7942_1823 0.39 -0.2 1,894,779 + +1.6 1,894,787 + nblB Synpcc7942_1823 0.43 -0.0 1,894,804 + nblB Synpcc7942_1823 0.46 -0.3 1,894,812 - nblB Synpcc7942_1823 0.47 -1.7 1,894,812 - nblB Synpcc7942_1823 0.47 +1.6 1,894,858 + nblB Synpcc7942_1823 0.54 +0.9 1,894,858 + nblB Synpcc7942_1823 0.54 -1.0 1,894,883 + nblB Synpcc7942_1823 0.58 -0.4 1,894,883 + nblB Synpcc7942_1823 0.58 +1.0 1,894,913 - nblB Synpcc7942_1823 0.63 -0.9 1,894,949 + nblB Synpcc7942_1823 0.68 -0.4 1,894,959 + nblB Synpcc7942_1823 0.70 -1.0 1,894,993 - nblB Synpcc7942_1823 0.75 -0.2 1,895,014 - nblB Synpcc7942_1823 0.78 -0.6 1,895,120 - -0.4 1,895,127 + -0.5 1,895,128 - -0.1 1,895,171 - -0.6 1,895,190 + -0.0 1,895,190 + -0.6 1,895,192 + +0.6 1,895,208 - -1.0 1,895,230 + -0.7 1,895,230 + Synpcc7942_1824 0.14 -0.4 1,895,230 + Synpcc7942_1824 0.14 -0.4 1,895,230 + Synpcc7942_1824 0.14 +0.6 1,895,230 + Synpcc7942_1824 0.14 +1.6 1,895,230 + Synpcc7942_1824 0.14 -1.4 1,895,238 - Synpcc7942_1824 0.15 -0.9 1,895,238 - Synpcc7942_1824 0.15 +1.6 1,895,238 - Synpcc7942_1824 0.15 -1.9 1,895,321 - Synpcc7942_1824 0.33 -2.1 1,895,321 - -0.9 1,895,329 + Synpcc7942_1824 0.35 +0.6 1,895,329 + Synpcc7942_1824 0.35 -0.4 1,895,337 - Synpcc7942_1824 0.37 -2.1 1,895,347 + Synpcc7942_1824 0.39 -0.5 1,895,352 + Synpcc7942_1824 0.40 -1.9 1,895,377 - Synpcc7942_1824 0.45 -0.2 1,895,402 + Synpcc7942_1824 0.51 -0.6 1,895,402 + Synpcc7942_1824 0.51 -0.7 1,895,420 + Synpcc7942_1824 0.55 -1.3 1,895,420 + Synpcc7942_1824 0.55 -2.0 1,895,444 + Synpcc7942_1824 0.60 -1.2 1,895,457 - Synpcc7942_1824 0.63 -2.1 1,895,458 + Synpcc7942_1824 0.63 -0.2 1,895,463 + Synpcc7942_1824 0.64 -0.0 1,895,479 + Synpcc7942_1824 0.67 -0.7 1,895,493 - Synpcc7942_1824 0.70 +1.0 1,895,501 - Synpcc7942_1824 0.72 -0.5 1,895,511 - Synpcc7942_1824 0.74 -1.9 1,895,522 + Synpcc7942_1824 0.77 -0.8 1,895,524 + Synpcc7942_1824 0.77 -0.6 1,895,524 + Synpcc7942_1824 0.77 -0.9 1,895,529 + Synpcc7942_1824 0.78 -1.0 1,895,535 - Synpcc7942_1824 0.79 -2.6 1,895,571 + Synpcc7942_1824 0.87 -2.2 1,895,572 + Synpcc7942_1824 0.87 +1.6 1,895,572 + Synpcc7942_1824 0.87 -1.9 1,895,574 - Synpcc7942_1824 0.88 +0.6 1,895,580 - Synpcc7942_1824 0.89 -1.9 1,895,595 - -1.1 1,895,597 - -0.7 1,895,597 - +0.7 1,895,602 - -0.7 1,895,626 + -0.3 1,895,634 - +0.5 1,895,637 + -0.4 1,895,637 + -0.0 1,895,638 + +1.6 1,895,638 + -0.0 1,895,646 - +0.6 1,895,647 - -1.0 1,895,647 - -1.3 1,895,649 + -1.6 1,895,718 - -0.3 1,895,722 - +0.3 1,895,813 + PgsA Synpcc7942_1825 0.20 +1.6 1,896,215 + -0.4 1,896,229 + -1.5 1,896,237 - +0.4 1,896,248 + +0.8 1,896,260 - -0.2 1,896,284 + -0.7 1,896,284 + +1.6 1,896,317 - Synpcc7942_1826 0.10 +1.6 1,896,368 - Synpcc7942_1826 0.15 -2.7 1,896,399 - Synpcc7942_1826 0.19 -0.7 1,896,421 + Synpcc7942_1826 0.21 +0.0 1,896,421 + Synpcc7942_1826 0.21 +1.0 1,896,421 + Synpcc7942_1826 0.21 -0.3 1,896,421 + Synpcc7942_1826 0.21 -1.2 1,896,439 - Synpcc7942_1826 0.23 -0.5 1,896,446 + Synpcc7942_1826 0.24 +0.3 1,896,446 + Synpcc7942_1826 0.24 +0.8 1,896,454 - Synpcc7942_1826 0.25 -0.1 1,896,476 - Synpcc7942_1826 0.27 -0.1 1,896,497 + Synpcc7942_1826 0.29 -0.0 1,896,507 + +0.2 1,896,507 + Synpcc7942_1826 0.30 -0.7 1,896,507 + Synpcc7942_1826 0.30 -0.4 1,896,507 + Synpcc7942_1826 0.30 -0.6 1,896,529 - Synpcc7942_1826 0.33 -0.3 1,896,552 + Synpcc7942_1826 0.35 +0.4 1,896,559 - Synpcc7942_1826 0.36 -1.1 1,896,561 - Synpcc7942_1826 0.36 -0.5 1,896,561 - Synpcc7942_1826 0.36 +0.5 1,896,572 + Synpcc7942_1826 0.37 -0.3 1,896,594 + Synpcc7942_1826 0.39 -0.4 1,896,612 + +1.6 1,896,612 + Synpcc7942_1826 0.41 +0.3 1,896,620 - Synpcc7942_1826 0.42 -0.0 1,896,627 + Synpcc7942_1826 0.43 -0.7
Or see this region's nucleotide sequence