Experiment: 9:9 LD Cycle Bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1287 and Synpcc7942_1288 are separated by 165 nucleotides Synpcc7942_1288 and pmbA are separated by 306 nucleotides pmbA and smtA are separated by 108 nucleotides smtA and smtB are separated by 100 nucleotides smtB and dnaG are separated by 9 nucleotides
Synpcc7942_1287: Synpcc7942_1287 - VCBS, at 1,309,030 to 1,310,862
_1287
Synpcc7942_1288: Synpcc7942_1288 - hypothetical protein, at 1,311,028 to 1,311,534
_1288
Synpcc7942_1289: pmbA - putative modulator of DNA gyrase, at 1,311,841 to 1,313,181
pmbA
Synpcc7942_1290: smtA - SmtA metallothionein, at 1,313,290 to 1,313,460
smtA
Synpcc7942_1291: smtB - transcriptional regulator, ArsR family, at 1,313,561 to 1,313,929
smtB
Synpcc7942_1292: dnaG - DNA primase, at 1,313,939 to 1,316,023
dnaG
Position (kb)
1311
1312
1313
1314 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1310.961 kb on + strand at 1310.969 kb on - strand at 1311.018 kb on - strand at 1311.061 kb on + strand at 1311.093 kb on + strand, within Synpcc7942_1288 at 1311.096 kb on - strand, within Synpcc7942_1288 at 1311.110 kb on + strand, within Synpcc7942_1288 at 1311.118 kb on - strand, within Synpcc7942_1288 at 1311.177 kb on + strand, within Synpcc7942_1288 at 1311.186 kb on + strand, within Synpcc7942_1288 at 1311.201 kb on + strand, within Synpcc7942_1288 at 1311.239 kb on + strand, within Synpcc7942_1288 at 1311.247 kb on - strand, within Synpcc7942_1288 at 1311.300 kb on - strand, within Synpcc7942_1288 at 1311.322 kb on - strand, within Synpcc7942_1288 at 1311.351 kb on + strand, within Synpcc7942_1288 at 1311.400 kb on - strand, within Synpcc7942_1288 at 1311.409 kb on + strand, within Synpcc7942_1288 at 1311.440 kb on + strand, within Synpcc7942_1288 at 1311.476 kb on + strand, within Synpcc7942_1288 at 1311.477 kb on - strand, within Synpcc7942_1288 at 1311.486 kb on + strand at 1311.513 kb on - strand at 1311.734 kb on + strand at 1311.744 kb on - strand at 1311.795 kb on + strand at 1311.845 kb on - strand at 1311.846 kb on - strand at 1311.852 kb on - strand at 1311.852 kb on - strand at 1311.876 kb on + strand at 1311.894 kb on - strand at 1311.904 kb on + strand at 1311.912 kb on - strand at 1311.912 kb on - strand at 1311.916 kb on + strand at 1311.918 kb on + strand at 1311.951 kb on + strand at 1311.965 kb on + strand at 1311.965 kb on + strand at 1311.973 kb on - strand at 1311.973 kb on - strand at 1311.975 kb on + strand, within pmbA at 1311.983 kb on - strand, within pmbA at 1311.990 kb on + strand, within pmbA at 1312.032 kb on + strand, within pmbA at 1312.040 kb on - strand, within pmbA at 1312.151 kb on + strand, within pmbA at 1312.191 kb on + strand, within pmbA at 1312.227 kb on - strand, within pmbA at 1312.237 kb on - strand, within pmbA at 1312.283 kb on - strand, within pmbA at 1312.310 kb on + strand, within pmbA at 1312.325 kb on + strand, within pmbA at 1312.377 kb on - strand, within pmbA at 1312.412 kb on + strand, within pmbA at 1312.412 kb on + strand, within pmbA at 1312.412 kb on + strand, within pmbA at 1312.420 kb on - strand, within pmbA at 1312.420 kb on - strand, within pmbA at 1312.472 kb on - strand, within pmbA at 1312.520 kb on + strand, within pmbA at 1312.520 kb on + strand, within pmbA at 1312.533 kb on - strand, within pmbA at 1312.602 kb on + strand, within pmbA at 1312.610 kb on - strand, within pmbA at 1312.610 kb on - strand, within pmbA at 1312.623 kb on - strand, within pmbA at 1312.654 kb on - strand, within pmbA at 1312.668 kb on + strand, within pmbA at 1312.676 kb on - strand, within pmbA at 1312.696 kb on - strand, within pmbA at 1312.706 kb on - strand, within pmbA at 1312.793 kb on + strand, within pmbA at 1312.801 kb on - strand, within pmbA at 1312.825 kb on - strand, within pmbA at 1312.881 kb on + strand, within pmbA at 1312.914 kb on - strand, within pmbA at 1312.925 kb on - strand, within pmbA at 1312.928 kb on + strand, within pmbA at 1312.962 kb on + strand, within pmbA at 1312.970 kb on - strand, within pmbA at 1313.080 kb on + strand at 1313.085 kb on + strand at 1313.118 kb on + strand at 1313.126 kb on - strand at 1313.151 kb on + strand at 1313.182 kb on + strand at 1313.182 kb on + strand at 1313.184 kb on + strand at 1313.185 kb on + strand at 1313.185 kb on + strand at 1313.193 kb on - strand at 1313.193 kb on - strand at 1313.193 kb on - strand at 1313.193 kb on - strand at 1313.198 kb on + strand at 1313.206 kb on - strand at 1313.230 kb on + strand at 1313.252 kb on - strand at 1313.291 kb on - strand at 1313.356 kb on + strand, within smtA at 1313.366 kb on + strand, within smtA at 1313.370 kb on + strand, within smtA at 1313.371 kb on + strand, within smtA at 1313.416 kb on - strand at 1313.424 kb on + strand, within smtA at 1313.440 kb on + strand, within smtA at 1313.455 kb on + strand at 1313.457 kb on - strand at 1313.563 kb on + strand at 1313.586 kb on + strand at 1313.589 kb on - strand at 1313.594 kb on + strand at 1313.625 kb on + strand, within smtB at 1313.665 kb on - strand, within smtB at 1313.684 kb on + strand, within smtB at 1313.692 kb on - strand, within smtB at 1313.716 kb on + strand, within smtB at 1313.716 kb on + strand, within smtB at 1313.716 kb on + strand, within smtB at 1313.716 kb on + strand, within smtB at 1313.737 kb on + strand, within smtB at 1313.740 kb on + strand, within smtB at 1313.750 kb on - strand, within smtB at 1313.761 kb on + strand, within smtB at 1313.763 kb on + strand, within smtB at 1313.814 kb on + strand, within smtB at 1313.852 kb on + strand, within smtB at 1313.852 kb on + strand, within smtB at 1313.857 kb on + strand, within smtB at 1313.860 kb on - strand, within smtB at 1313.878 kb on - strand, within smtB at 1313.936 kb on + strand at 1313.937 kb on + strand at 1313.977 kb on + strand at 1313.977 kb on + strand at 1313.979 kb on + strand at 1313.984 kb on + strand at 1313.985 kb on - strand at 1314.002 kb on - strand at 1314.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 9:9 LD Cycle Bioreactor remove 1,310,961 + +1.5 1,310,969 - +0.5 1,311,018 - +0.5 1,311,061 + +0.5 1,311,093 + Synpcc7942_1288 0.13 -0.4 1,311,096 - Synpcc7942_1288 0.13 +0.1 1,311,110 + Synpcc7942_1288 0.16 +0.5 1,311,118 - Synpcc7942_1288 0.18 -0.1 1,311,177 + Synpcc7942_1288 0.29 -0.7 1,311,186 + Synpcc7942_1288 0.31 -0.7 1,311,201 + Synpcc7942_1288 0.34 +0.1 1,311,239 + Synpcc7942_1288 0.42 -1.1 1,311,247 - Synpcc7942_1288 0.43 -0.6 1,311,300 - Synpcc7942_1288 0.54 +0.5 1,311,322 - Synpcc7942_1288 0.58 +1.5 1,311,351 + Synpcc7942_1288 0.64 +1.1 1,311,400 - Synpcc7942_1288 0.73 +0.4 1,311,409 + Synpcc7942_1288 0.75 -0.2 1,311,440 + Synpcc7942_1288 0.81 -1.5 1,311,476 + Synpcc7942_1288 0.88 +0.5 1,311,477 - Synpcc7942_1288 0.89 +0.5 1,311,486 + -1.9 1,311,513 - -0.6 1,311,734 + +1.5 1,311,744 - -0.0 1,311,795 + -0.1 1,311,845 - -1.3 1,311,846 - -1.1 1,311,852 - -0.1 1,311,852 - -1.1 1,311,876 + -0.1 1,311,894 - +1.5 1,311,904 + -0.5 1,311,912 - +0.8 1,311,912 - -0.5 1,311,916 + +0.3 1,311,918 + +0.1 1,311,951 + +1.5 1,311,965 + -1.2 1,311,965 + -0.1 1,311,973 - +0.2 1,311,973 - -0.3 1,311,975 + pmbA Synpcc7942_1289 0.10 -1.3 1,311,983 - pmbA Synpcc7942_1289 0.11 -0.1 1,311,990 + pmbA Synpcc7942_1289 0.11 +0.5 1,312,032 + pmbA Synpcc7942_1289 0.14 -1.7 1,312,040 - pmbA Synpcc7942_1289 0.15 -2.1 1,312,151 + pmbA Synpcc7942_1289 0.23 +1.5 1,312,191 + pmbA Synpcc7942_1289 0.26 -0.3 1,312,227 - pmbA Synpcc7942_1289 0.29 -2.1 1,312,237 - pmbA Synpcc7942_1289 0.30 -0.1 1,312,283 - pmbA Synpcc7942_1289 0.33 +0.5 1,312,310 + pmbA Synpcc7942_1289 0.35 -0.8 1,312,325 + pmbA Synpcc7942_1289 0.36 +0.5 1,312,377 - pmbA Synpcc7942_1289 0.40 +0.2 1,312,412 + pmbA Synpcc7942_1289 0.43 +1.5 1,312,412 + pmbA Synpcc7942_1289 0.43 -0.3 1,312,412 + pmbA Synpcc7942_1289 0.43 -3.5 1,312,420 - pmbA Synpcc7942_1289 0.43 +0.5 1,312,420 - pmbA Synpcc7942_1289 0.43 -0.7 1,312,472 - pmbA Synpcc7942_1289 0.47 -0.1 1,312,520 + pmbA Synpcc7942_1289 0.51 +0.5 1,312,520 + pmbA Synpcc7942_1289 0.51 -0.6 1,312,533 - pmbA Synpcc7942_1289 0.52 -1.3 1,312,602 + pmbA Synpcc7942_1289 0.57 +0.5 1,312,610 - pmbA Synpcc7942_1289 0.57 +0.9 1,312,610 - pmbA Synpcc7942_1289 0.57 +1.5 1,312,623 - pmbA Synpcc7942_1289 0.58 +1.0 1,312,654 - pmbA Synpcc7942_1289 0.61 +1.5 1,312,668 + pmbA Synpcc7942_1289 0.62 -0.5 1,312,676 - pmbA Synpcc7942_1289 0.62 -0.1 1,312,696 - pmbA Synpcc7942_1289 0.64 -0.5 1,312,706 - pmbA Synpcc7942_1289 0.65 -0.7 1,312,793 + pmbA Synpcc7942_1289 0.71 -1.3 1,312,801 - pmbA Synpcc7942_1289 0.72 -0.1 1,312,825 - pmbA Synpcc7942_1289 0.73 -1.3 1,312,881 + pmbA Synpcc7942_1289 0.78 -0.2 1,312,914 - pmbA Synpcc7942_1289 0.80 +0.2 1,312,925 - pmbA Synpcc7942_1289 0.81 +0.8 1,312,928 + pmbA Synpcc7942_1289 0.81 -1.7 1,312,962 + pmbA Synpcc7942_1289 0.84 -1.1 1,312,970 - pmbA Synpcc7942_1289 0.84 -0.5 1,313,080 + -0.1 1,313,085 + +1.5 1,313,118 + +0.5 1,313,126 - -1.5 1,313,151 + +0.9 1,313,182 + +1.3 1,313,182 + +0.3 1,313,184 + +2.1 1,313,185 + +0.1 1,313,185 + -0.6 1,313,193 - +0.8 1,313,193 - +0.5 1,313,193 - -0.4 1,313,193 - -2.5 1,313,198 + -1.9 1,313,206 - -0.9 1,313,230 + -0.0 1,313,252 - -0.7 1,313,291 - +0.5 1,313,356 + smtA Synpcc7942_1290 0.39 -0.4 1,313,366 + smtA Synpcc7942_1290 0.44 -0.1 1,313,370 + smtA Synpcc7942_1290 0.47 +1.5 1,313,371 + smtA Synpcc7942_1290 0.47 -0.3 1,313,416 - +0.7 1,313,424 + smtA Synpcc7942_1290 0.78 -1.5 1,313,440 + smtA Synpcc7942_1290 0.88 +1.5 1,313,455 + -1.4 1,313,457 - +1.5 1,313,563 + -0.6 1,313,586 + +0.1 1,313,589 - +0.2 1,313,594 + +0.9 1,313,625 + smtB Synpcc7942_1291 0.17 +0.4 1,313,665 - smtB Synpcc7942_1291 0.28 +0.2 1,313,684 + smtB Synpcc7942_1291 0.33 +0.2 1,313,692 - smtB Synpcc7942_1291 0.36 -0.1 1,313,716 + smtB Synpcc7942_1291 0.42 +0.3 1,313,716 + smtB Synpcc7942_1291 0.42 +0.9 1,313,716 + smtB Synpcc7942_1291 0.42 -0.3 1,313,716 + smtB Synpcc7942_1291 0.42 +1.5 1,313,737 + smtB Synpcc7942_1291 0.48 -0.1 1,313,740 + smtB Synpcc7942_1291 0.49 -0.8 1,313,750 - smtB Synpcc7942_1291 0.51 +0.5 1,313,761 + smtB Synpcc7942_1291 0.54 +1.1 1,313,763 + smtB Synpcc7942_1291 0.55 +0.5 1,313,814 + smtB Synpcc7942_1291 0.69 -0.1 1,313,852 + smtB Synpcc7942_1291 0.79 -0.5 1,313,852 + smtB Synpcc7942_1291 0.79 +1.5 1,313,857 + smtB Synpcc7942_1291 0.80 -0.3 1,313,860 - smtB Synpcc7942_1291 0.81 -0.1 1,313,878 - smtB Synpcc7942_1291 0.86 +0.7 1,313,936 + +0.5 1,313,937 + -0.5 1,313,977 + +3.1 1,313,977 + +0.1 1,313,979 + -0.4 1,313,984 + +0.0 1,313,985 - +0.5 1,314,002 - +1.5 1,314,007 - -1.3
Or see this region's nucleotide sequence