Experiment: 9:9 LD Cycle Bioreactor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cytf and petC are separated by 45 nucleotides petC and Synpcc7942_1233 are separated by 300 nucleotides Synpcc7942_1233 and Synpcc7942_1234 are separated by 265 nucleotides
Synpcc7942_1231: cytf - cytochrome b6f complex subunit PetA, at 1,251,930 to 1,252,904
cytf
Synpcc7942_1232: petC - cytochrome b6-f complex iron-sulfur subunit, at 1,252,950 to 1,253,489
petC
Synpcc7942_1233: Synpcc7942_1233 - hypothetical protein, at 1,253,790 to 1,254,473
_1233
Synpcc7942_1234: Synpcc7942_1234 - hypothetical protein, at 1,254,739 to 1,255,242
_1234
Position (kb)
1253
1254
1255 Strain fitness (log2 ratio)
-2
-1
0
1 at 1253.692 kb on + strand at 1253.692 kb on + strand at 1253.693 kb on - strand at 1253.700 kb on - strand at 1253.714 kb on - strand at 1253.714 kb on - strand at 1253.736 kb on - strand at 1253.746 kb on + strand at 1253.762 kb on + strand at 1253.762 kb on + strand at 1253.770 kb on - strand at 1253.772 kb on - strand at 1253.786 kb on + strand at 1253.786 kb on + strand at 1253.794 kb on - strand at 1253.796 kb on - strand at 1253.859 kb on - strand, within Synpcc7942_1233 at 1253.873 kb on + strand, within Synpcc7942_1233 at 1253.940 kb on - strand, within Synpcc7942_1233 at 1254.041 kb on - strand, within Synpcc7942_1233 at 1254.082 kb on + strand, within Synpcc7942_1233 at 1254.090 kb on - strand, within Synpcc7942_1233 at 1254.092 kb on + strand, within Synpcc7942_1233 at 1254.221 kb on - strand, within Synpcc7942_1233 at 1254.378 kb on + strand, within Synpcc7942_1233 at 1254.537 kb on + strand at 1254.733 kb on - strand at 1254.735 kb on + strand at 1254.761 kb on - strand at 1254.799 kb on + strand, within Synpcc7942_1234 at 1254.801 kb on + strand, within Synpcc7942_1234 at 1254.801 kb on + strand, within Synpcc7942_1234 at 1254.801 kb on + strand, within Synpcc7942_1234 at 1254.801 kb on + strand, within Synpcc7942_1234 at 1254.886 kb on + strand, within Synpcc7942_1234 at 1254.918 kb on + strand, within Synpcc7942_1234 at 1254.918 kb on + strand, within Synpcc7942_1234 at 1254.934 kb on - strand, within Synpcc7942_1234 at 1254.988 kb on - strand, within Synpcc7942_1234 at 1254.989 kb on + strand, within Synpcc7942_1234 at 1255.020 kb on + strand, within Synpcc7942_1234 at 1255.028 kb on - strand, within Synpcc7942_1234 at 1255.042 kb on + strand, within Synpcc7942_1234 at 1255.068 kb on - strand, within Synpcc7942_1234 at 1255.097 kb on + strand, within Synpcc7942_1234 at 1255.119 kb on - strand, within Synpcc7942_1234 at 1255.159 kb on + strand, within Synpcc7942_1234 at 1255.336 kb on + strand at 1255.338 kb on + strand at 1255.372 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 9:9 LD Cycle Bioreactor remove 1,253,692 + -0.7 1,253,692 + -0.1 1,253,693 - -0.2 1,253,700 - -1.3 1,253,714 - -1.5 1,253,714 - +0.2 1,253,736 - +0.8 1,253,746 + -0.1 1,253,762 + -1.7 1,253,762 + +0.0 1,253,770 - +0.4 1,253,772 - -0.7 1,253,786 + +1.5 1,253,786 + +0.2 1,253,794 - -0.4 1,253,796 - +0.3 1,253,859 - Synpcc7942_1233 0.10 -1.1 1,253,873 + Synpcc7942_1233 0.12 -0.3 1,253,940 - Synpcc7942_1233 0.22 -0.0 1,254,041 - Synpcc7942_1233 0.37 +1.5 1,254,082 + Synpcc7942_1233 0.43 -1.1 1,254,090 - Synpcc7942_1233 0.44 -1.3 1,254,092 + Synpcc7942_1233 0.44 -0.2 1,254,221 - Synpcc7942_1233 0.63 +1.1 1,254,378 + Synpcc7942_1233 0.86 +0.9 1,254,537 + +0.5 1,254,733 - -0.8 1,254,735 + -0.4 1,254,761 - -0.6 1,254,799 + Synpcc7942_1234 0.12 -0.1 1,254,801 + Synpcc7942_1234 0.12 -0.1 1,254,801 + Synpcc7942_1234 0.12 -0.3 1,254,801 + Synpcc7942_1234 0.12 +1.5 1,254,801 + Synpcc7942_1234 0.12 -0.5 1,254,886 + Synpcc7942_1234 0.29 +0.0 1,254,918 + Synpcc7942_1234 0.36 -0.5 1,254,918 + Synpcc7942_1234 0.36 -0.1 1,254,934 - Synpcc7942_1234 0.39 +1.5 1,254,988 - Synpcc7942_1234 0.49 +1.5 1,254,989 + Synpcc7942_1234 0.50 -0.8 1,255,020 + Synpcc7942_1234 0.56 +1.1 1,255,028 - Synpcc7942_1234 0.57 -1.2 1,255,042 + Synpcc7942_1234 0.60 -0.0 1,255,068 - Synpcc7942_1234 0.65 -0.5 1,255,097 + Synpcc7942_1234 0.71 -0.5 1,255,119 - Synpcc7942_1234 0.75 -0.7 1,255,159 + Synpcc7942_1234 0.83 -0.2 1,255,336 + -0.5 1,255,338 + -1.0 1,255,372 - -1.9
Or see this region's nucleotide sequence