Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_5072 and Psyr_5073 are separated by 52 nucleotides Psyr_5073 and Psyr_5074 are separated by 3 nucleotides Psyr_5074 and Psyr_5075 overlap by 1 nucleotides
Psyr_5072: Psyr_5072 - Cobalamin synthesis protein/P47K:Cobalamin synthesis protein/P47K, at 6,016,502 to 6,017,473
_5072
Psyr_5073: Psyr_5073 - conserved hypothetical protein, at 6,017,526 to 6,017,900
_5073
Psyr_5074: Psyr_5074 - conserved hypothetical protein, at 6,017,904 to 6,018,554
_5074
Psyr_5075: Psyr_5075 - Cobalamin synthesis protein/P47K:Cobalamin synthesis protein/P47K, at 6,018,554 to 6,019,762
_5075
Position (kb)
6017
6018
6019 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 6017.459 kb on + strand at 6017.460 kb on - strand at 6017.533 kb on + strand at 6017.533 kb on + strand at 6017.533 kb on + strand at 6017.534 kb on - strand at 6017.534 kb on - strand at 6017.534 kb on - strand at 6017.534 kb on - strand at 6017.534 kb on - strand at 6017.534 kb on - strand at 6017.534 kb on - strand at 6017.657 kb on + strand, within Psyr_5073 at 6017.658 kb on - strand, within Psyr_5073 at 6017.734 kb on - strand, within Psyr_5073 at 6017.792 kb on + strand, within Psyr_5073 at 6017.793 kb on - strand, within Psyr_5073 at 6017.793 kb on - strand, within Psyr_5073 at 6017.793 kb on - strand, within Psyr_5073 at 6017.820 kb on + strand, within Psyr_5073 at 6017.844 kb on + strand, within Psyr_5073 at 6017.844 kb on + strand, within Psyr_5073 at 6017.844 kb on + strand, within Psyr_5073 at 6017.845 kb on - strand, within Psyr_5073 at 6017.845 kb on - strand, within Psyr_5073 at 6018.139 kb on + strand, within Psyr_5074 at 6018.139 kb on + strand, within Psyr_5074 at 6018.140 kb on - strand, within Psyr_5074 at 6018.140 kb on - strand, within Psyr_5074 at 6018.250 kb on + strand, within Psyr_5074 at 6018.251 kb on - strand, within Psyr_5074 at 6018.423 kb on + strand, within Psyr_5074 at 6018.423 kb on + strand, within Psyr_5074 at 6018.423 kb on + strand, within Psyr_5074 at 6018.423 kb on + strand, within Psyr_5074 at 6018.423 kb on + strand, within Psyr_5074 at 6018.423 kb on + strand, within Psyr_5074 at 6018.423 kb on + strand, within Psyr_5074 at 6018.423 kb on + strand, within Psyr_5074 at 6018.423 kb on + strand, within Psyr_5074 at 6018.423 kb on + strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.424 kb on - strand, within Psyr_5074 at 6018.510 kb on + strand at 6018.510 kb on + strand at 6018.511 kb on - strand at 6018.830 kb on + strand, within Psyr_5075 at 6018.870 kb on + strand, within Psyr_5075 at 6018.870 kb on + strand, within Psyr_5075 at 6018.870 kb on + strand, within Psyr_5075 at 6018.870 kb on + strand, within Psyr_5075 at 6018.870 kb on + strand, within Psyr_5075 at 6018.870 kb on + strand, within Psyr_5075 at 6018.870 kb on + strand, within Psyr_5075 at 6018.870 kb on + strand, within Psyr_5075 at 6018.870 kb on + strand, within Psyr_5075 at 6018.870 kb on + strand, within Psyr_5075 at 6018.871 kb on - strand, within Psyr_5075 at 6018.871 kb on - strand, within Psyr_5075 at 6018.871 kb on - strand, within Psyr_5075 at 6018.871 kb on - strand, within Psyr_5075 at 6018.871 kb on - strand, within Psyr_5075 at 6018.871 kb on - strand, within Psyr_5075 at 6018.982 kb on - strand, within Psyr_5075 at 6019.002 kb on + strand, within Psyr_5075 at 6019.002 kb on + strand, within Psyr_5075 at 6019.003 kb on - strand, within Psyr_5075 at 6019.216 kb on + strand, within Psyr_5075 at 6019.216 kb on + strand, within Psyr_5075 at 6019.475 kb on + strand, within Psyr_5075 at 6019.475 kb on + strand, within Psyr_5075 at 6019.475 kb on + strand, within Psyr_5075 at 6019.475 kb on + strand, within Psyr_5075 at 6019.476 kb on - strand, within Psyr_5075 at 6019.476 kb on - strand, within Psyr_5075 at 6019.476 kb on - strand, within Psyr_5075 at 6019.480 kb on - strand, within Psyr_5075 at 6019.480 kb on - strand, within Psyr_5075 at 6019.480 kb on - strand, within Psyr_5075
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 6,017,459 + +0.3 6,017,460 - -0.1 6,017,533 + -1.2 6,017,533 + -1.4 6,017,533 + -1.2 6,017,534 - +0.7 6,017,534 - -0.1 6,017,534 - +0.1 6,017,534 - -0.3 6,017,534 - -0.1 6,017,534 - -0.4 6,017,534 - -0.0 6,017,657 + Psyr_5073 0.35 +1.7 6,017,658 - Psyr_5073 0.35 +0.4 6,017,734 - Psyr_5073 0.55 -1.2 6,017,792 + Psyr_5073 0.71 -0.4 6,017,793 - Psyr_5073 0.71 +0.6 6,017,793 - Psyr_5073 0.71 -0.3 6,017,793 - Psyr_5073 0.71 +0.4 6,017,820 + Psyr_5073 0.78 -1.2 6,017,844 + Psyr_5073 0.85 +0.8 6,017,844 + Psyr_5073 0.85 +2.0 6,017,844 + Psyr_5073 0.85 +0.1 6,017,845 - Psyr_5073 0.85 +0.4 6,017,845 - Psyr_5073 0.85 +1.8 6,018,139 + Psyr_5074 0.36 -1.2 6,018,139 + Psyr_5074 0.36 +0.4 6,018,140 - Psyr_5074 0.36 +1.4 6,018,140 - Psyr_5074 0.36 +2.5 6,018,250 + Psyr_5074 0.53 +2.0 6,018,251 - Psyr_5074 0.53 +0.5 6,018,423 + Psyr_5074 0.80 +4.0 6,018,423 + Psyr_5074 0.80 +0.5 6,018,423 + Psyr_5074 0.80 +0.2 6,018,423 + Psyr_5074 0.80 +0.1 6,018,423 + Psyr_5074 0.80 +1.4 6,018,423 + Psyr_5074 0.80 +2.0 6,018,423 + Psyr_5074 0.80 +1.2 6,018,423 + Psyr_5074 0.80 +1.2 6,018,423 + Psyr_5074 0.80 +0.0 6,018,423 + Psyr_5074 0.80 -1.1 6,018,424 - Psyr_5074 0.80 -0.8 6,018,424 - Psyr_5074 0.80 -1.0 6,018,424 - Psyr_5074 0.80 -3.5 6,018,424 - Psyr_5074 0.80 -0.0 6,018,424 - Psyr_5074 0.80 +0.7 6,018,424 - Psyr_5074 0.80 -0.5 6,018,424 - Psyr_5074 0.80 +1.3 6,018,424 - Psyr_5074 0.80 -0.1 6,018,424 - Psyr_5074 0.80 -0.6 6,018,424 - Psyr_5074 0.80 -0.5 6,018,424 - Psyr_5074 0.80 +0.1 6,018,510 + +0.6 6,018,510 + -0.6 6,018,511 - -0.5 6,018,830 + Psyr_5075 0.23 -0.5 6,018,870 + Psyr_5075 0.26 -0.5 6,018,870 + Psyr_5075 0.26 +0.0 6,018,870 + Psyr_5075 0.26 -2.3 6,018,870 + Psyr_5075 0.26 -0.3 6,018,870 + Psyr_5075 0.26 -1.4 6,018,870 + Psyr_5075 0.26 +1.4 6,018,870 + Psyr_5075 0.26 -0.2 6,018,870 + Psyr_5075 0.26 -2.3 6,018,870 + Psyr_5075 0.26 +4.4 6,018,870 + Psyr_5075 0.26 -0.2 6,018,871 - Psyr_5075 0.26 +0.1 6,018,871 - Psyr_5075 0.26 -0.2 6,018,871 - Psyr_5075 0.26 +0.8 6,018,871 - Psyr_5075 0.26 -0.1 6,018,871 - Psyr_5075 0.26 -0.7 6,018,871 - Psyr_5075 0.26 +2.4 6,018,982 - Psyr_5075 0.35 +0.1 6,019,002 + Psyr_5075 0.37 -0.1 6,019,002 + Psyr_5075 0.37 -0.7 6,019,003 - Psyr_5075 0.37 -1.9 6,019,216 + Psyr_5075 0.55 +0.4 6,019,216 + Psyr_5075 0.55 -0.9 6,019,475 + Psyr_5075 0.76 +0.2 6,019,475 + Psyr_5075 0.76 +0.2 6,019,475 + Psyr_5075 0.76 -0.2 6,019,475 + Psyr_5075 0.76 +0.7 6,019,476 - Psyr_5075 0.76 -0.4 6,019,476 - Psyr_5075 0.76 -0.7 6,019,476 - Psyr_5075 0.76 -0.8 6,019,480 - Psyr_5075 0.77 +0.4 6,019,480 - Psyr_5075 0.77 -0.1 6,019,480 - Psyr_5075 0.77 +0.5
Or see this region's nucleotide sequence