Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3913 and Psyr_3914 are separated by 219 nucleotides Psyr_3914 and Psyr_3915 are separated by 67 nucleotides Psyr_3915 and Psyr_3916 are separated by 123 nucleotides Psyr_3916 and Psyr_3917 are separated by 43 nucleotides
Psyr_3913: Psyr_3913 - Helix-turn-helix, Fis-type, at 4,656,319 to 4,657,647
_3913
Psyr_3914: Psyr_3914 - DSBA oxidoreductase, at 4,657,867 to 4,658,514
_3914
Psyr_3915: Psyr_3915 - Alpha/beta hydrolase fold protein, at 4,658,582 to 4,659,574
_3915
Psyr_3916: Psyr_3916 - methionine synthase (B12-independent), at 4,659,698 to 4,660,495
_3916
Psyr_3917: Psyr_3917 - transcriptional regulator, LysR family, at 4,660,539 to 4,661,456
_3917
Position (kb) 4658
4659
4660 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4657.778 kb on + strand at 4657.848 kb on - strand at 4657.869 kb on - strand at 4657.910 kb on - strand at 4657.910 kb on - strand at 4657.910 kb on - strand at 4657.940 kb on + strand, within Psyr_3914 at 4657.941 kb on - strand, within Psyr_3914 at 4657.941 kb on - strand, within Psyr_3914 at 4657.941 kb on - strand, within Psyr_3914 at 4658.024 kb on + strand, within Psyr_3914 at 4658.024 kb on + strand, within Psyr_3914 at 4658.132 kb on + strand, within Psyr_3914 at 4658.132 kb on + strand, within Psyr_3914 at 4658.133 kb on - strand, within Psyr_3914 at 4658.133 kb on - strand, within Psyr_3914 at 4658.133 kb on - strand, within Psyr_3914 at 4658.219 kb on + strand, within Psyr_3914 at 4658.219 kb on + strand, within Psyr_3914 at 4658.219 kb on + strand, within Psyr_3914 at 4658.219 kb on + strand, within Psyr_3914 at 4658.219 kb on + strand, within Psyr_3914 at 4658.219 kb on + strand, within Psyr_3914 at 4658.220 kb on - strand, within Psyr_3914 at 4658.220 kb on - strand, within Psyr_3914 at 4658.220 kb on - strand, within Psyr_3914 at 4658.220 kb on - strand, within Psyr_3914 at 4658.220 kb on - strand, within Psyr_3914 at 4658.220 kb on - strand, within Psyr_3914 at 4658.220 kb on - strand, within Psyr_3914 at 4658.220 kb on - strand, within Psyr_3914 at 4658.224 kb on - strand, within Psyr_3914 at 4658.289 kb on + strand, within Psyr_3914 at 4658.289 kb on + strand, within Psyr_3914 at 4658.289 kb on + strand, within Psyr_3914 at 4658.289 kb on + strand, within Psyr_3914 at 4658.290 kb on - strand, within Psyr_3914 at 4658.290 kb on - strand, within Psyr_3914 at 4658.318 kb on + strand, within Psyr_3914 at 4658.530 kb on + strand at 4658.548 kb on + strand at 4658.549 kb on - strand at 4658.549 kb on - strand at 4658.584 kb on - strand at 4658.584 kb on - strand at 4659.040 kb on + strand, within Psyr_3915 at 4659.040 kb on + strand, within Psyr_3915 at 4659.041 kb on - strand, within Psyr_3915 at 4659.223 kb on - strand, within Psyr_3915 at 4659.223 kb on - strand, within Psyr_3915 at 4659.252 kb on + strand, within Psyr_3915 at 4659.448 kb on + strand, within Psyr_3915 at 4659.448 kb on + strand, within Psyr_3915 at 4659.448 kb on + strand, within Psyr_3915 at 4659.456 kb on + strand, within Psyr_3915 at 4659.491 kb on + strand at 4659.491 kb on + strand at 4659.491 kb on + strand at 4659.492 kb on - strand at 4659.602 kb on + strand at 4659.692 kb on - strand at 4659.694 kb on + strand at 4659.694 kb on + strand at 4659.694 kb on + strand at 4659.694 kb on + strand at 4659.694 kb on + strand at 4659.694 kb on + strand at 4659.694 kb on + strand at 4659.694 kb on + strand at 4659.694 kb on + strand at 4659.694 kb on + strand at 4659.695 kb on - strand at 4659.695 kb on - strand at 4659.695 kb on - strand at 4659.695 kb on - strand at 4659.695 kb on - strand at 4659.695 kb on - strand at 4659.695 kb on - strand at 4659.695 kb on - strand at 4659.695 kb on - strand at 4659.695 kb on - strand at 4659.700 kb on - strand at 4659.700 kb on - strand at 4659.787 kb on + strand, within Psyr_3916 at 4659.788 kb on - strand, within Psyr_3916 at 4659.816 kb on + strand, within Psyr_3916 at 4659.816 kb on + strand, within Psyr_3916 at 4659.816 kb on + strand, within Psyr_3916 at 4659.816 kb on + strand, within Psyr_3916 at 4659.817 kb on - strand, within Psyr_3916 at 4659.817 kb on - strand, within Psyr_3916 at 4659.966 kb on + strand, within Psyr_3916 at 4659.967 kb on - strand, within Psyr_3916 at 4660.095 kb on + strand, within Psyr_3916 at 4660.095 kb on + strand, within Psyr_3916 at 4660.096 kb on - strand, within Psyr_3916 at 4660.133 kb on + strand, within Psyr_3916 at 4660.133 kb on + strand, within Psyr_3916 at 4660.133 kb on + strand, within Psyr_3916 at 4660.133 kb on + strand, within Psyr_3916 at 4660.134 kb on - strand, within Psyr_3916 at 4660.134 kb on - strand, within Psyr_3916 at 4660.157 kb on + strand, within Psyr_3916 at 4660.158 kb on - strand, within Psyr_3916 at 4660.158 kb on - strand, within Psyr_3916 at 4660.336 kb on + strand, within Psyr_3916 at 4660.337 kb on - strand, within Psyr_3916 at 4660.407 kb on + strand, within Psyr_3916 at 4660.446 kb on + strand at 4660.447 kb on - strand at 4660.549 kb on + strand at 4660.549 kb on + strand at 4660.549 kb on + strand at 4660.550 kb on - strand at 4660.550 kb on - strand at 4660.550 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 4,657,778 + -2.3 4,657,848 - -1.7 4,657,869 - -1.7 4,657,910 - +1.3 4,657,910 - +0.2 4,657,910 - -0.5 4,657,940 + Psyr_3914 0.11 -0.2 4,657,941 - Psyr_3914 0.11 -0.6 4,657,941 - Psyr_3914 0.11 -0.1 4,657,941 - Psyr_3914 0.11 +3.8 4,658,024 + Psyr_3914 0.24 -1.2 4,658,024 + Psyr_3914 0.24 -0.2 4,658,132 + Psyr_3914 0.41 -1.0 4,658,132 + Psyr_3914 0.41 -0.7 4,658,133 - Psyr_3914 0.41 +0.9 4,658,133 - Psyr_3914 0.41 -1.8 4,658,133 - Psyr_3914 0.41 -0.3 4,658,219 + Psyr_3914 0.54 -0.2 4,658,219 + Psyr_3914 0.54 -1.4 4,658,219 + Psyr_3914 0.54 +0.8 4,658,219 + Psyr_3914 0.54 -1.2 4,658,219 + Psyr_3914 0.54 -0.2 4,658,219 + Psyr_3914 0.54 -0.2 4,658,220 - Psyr_3914 0.54 +0.6 4,658,220 - Psyr_3914 0.54 -1.6 4,658,220 - Psyr_3914 0.54 -0.3 4,658,220 - Psyr_3914 0.54 +0.4 4,658,220 - Psyr_3914 0.54 +0.7 4,658,220 - Psyr_3914 0.54 -2.7 4,658,220 - Psyr_3914 0.54 +0.2 4,658,220 - Psyr_3914 0.54 -0.3 4,658,224 - Psyr_3914 0.55 +2.0 4,658,289 + Psyr_3914 0.65 +3.6 4,658,289 + Psyr_3914 0.65 +1.0 4,658,289 + Psyr_3914 0.65 +0.9 4,658,289 + Psyr_3914 0.65 +3.0 4,658,290 - Psyr_3914 0.65 +0.2 4,658,290 - Psyr_3914 0.65 +4.0 4,658,318 + Psyr_3914 0.70 -0.5 4,658,530 + -1.2 4,658,548 + +0.7 4,658,549 - +0.2 4,658,549 - +0.2 4,658,584 - -1.1 4,658,584 - -0.7 4,659,040 + Psyr_3915 0.46 +1.0 4,659,040 + Psyr_3915 0.46 +0.8 4,659,041 - Psyr_3915 0.46 +1.5 4,659,223 - Psyr_3915 0.65 +0.2 4,659,223 - Psyr_3915 0.65 +0.7 4,659,252 + Psyr_3915 0.67 -0.4 4,659,448 + Psyr_3915 0.87 -2.2 4,659,448 + Psyr_3915 0.87 +0.6 4,659,448 + Psyr_3915 0.87 +1.0 4,659,456 + Psyr_3915 0.88 -0.9 4,659,491 + +1.3 4,659,491 + +0.2 4,659,491 + +0.4 4,659,492 - +1.0 4,659,602 + -1.3 4,659,692 - -0.1 4,659,694 + -0.8 4,659,694 + +0.9 4,659,694 + +0.8 4,659,694 + +1.0 4,659,694 + +0.8 4,659,694 + -0.2 4,659,694 + -1.0 4,659,694 + +0.8 4,659,694 + -0.8 4,659,694 + -0.6 4,659,695 - -1.3 4,659,695 - +0.2 4,659,695 - -0.3 4,659,695 - -0.6 4,659,695 - -1.0 4,659,695 - -1.1 4,659,695 - +0.6 4,659,695 - +0.0 4,659,695 - +0.2 4,659,695 - +0.5 4,659,700 - -1.4 4,659,700 - +0.0 4,659,787 + Psyr_3916 0.11 +0.8 4,659,788 - Psyr_3916 0.11 -0.0 4,659,816 + Psyr_3916 0.15 -0.6 4,659,816 + Psyr_3916 0.15 -0.1 4,659,816 + Psyr_3916 0.15 +1.3 4,659,816 + Psyr_3916 0.15 -1.0 4,659,817 - Psyr_3916 0.15 +2.0 4,659,817 - Psyr_3916 0.15 +1.0 4,659,966 + Psyr_3916 0.34 +0.4 4,659,967 - Psyr_3916 0.34 -0.5 4,660,095 + Psyr_3916 0.50 -0.8 4,660,095 + Psyr_3916 0.50 -0.3 4,660,096 - Psyr_3916 0.50 -1.6 4,660,133 + Psyr_3916 0.55 -0.1 4,660,133 + Psyr_3916 0.55 +0.8 4,660,133 + Psyr_3916 0.55 -1.8 4,660,133 + Psyr_3916 0.55 -0.2 4,660,134 - Psyr_3916 0.55 -0.7 4,660,134 - Psyr_3916 0.55 +0.0 4,660,157 + Psyr_3916 0.58 +1.6 4,660,158 - Psyr_3916 0.58 +0.3 4,660,158 - Psyr_3916 0.58 +0.1 4,660,336 + Psyr_3916 0.80 +0.2 4,660,337 - Psyr_3916 0.80 +1.8 4,660,407 + Psyr_3916 0.89 +0.6 4,660,446 + +0.2 4,660,447 - -1.3 4,660,549 + +0.4 4,660,549 + +1.0 4,660,549 + -0.6 4,660,550 - +2.3 4,660,550 - +1.0 4,660,550 - -0.9
Or see this region's nucleotide sequence