Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3747 and Psyr_3748 are separated by 3 nucleotides Psyr_3748 and Psyr_3749 are separated by 79 nucleotides
Psyr_3747: Psyr_3747 - asparagine synthase (glutamine-hydrolysing), at 4,464,461 to 4,466,233
_3747
Psyr_3748: Psyr_3748 - GCN5-related N-acetyltransferase, at 4,466,237 to 4,467,985
_3748
Psyr_3749: Psyr_3749 - Peptidase M42, at 4,468,065 to 4,469,261
_3749
Position (kb)
4466
4467
4468 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4465.520 kb on + strand, within Psyr_3747 at 4465.520 kb on + strand, within Psyr_3747 at 4465.521 kb on - strand, within Psyr_3747 at 4465.560 kb on + strand, within Psyr_3747 at 4465.599 kb on - strand, within Psyr_3747 at 4465.682 kb on + strand, within Psyr_3747 at 4465.682 kb on + strand, within Psyr_3747 at 4465.682 kb on + strand, within Psyr_3747 at 4466.019 kb on - strand, within Psyr_3747 at 4466.062 kb on + strand at 4466.063 kb on - strand at 4466.258 kb on + strand at 4466.258 kb on + strand at 4466.258 kb on + strand at 4466.258 kb on + strand at 4466.259 kb on - strand at 4466.259 kb on - strand at 4466.260 kb on + strand at 4466.260 kb on + strand at 4466.294 kb on + strand at 4466.295 kb on - strand at 4466.295 kb on - strand at 4466.337 kb on + strand at 4466.337 kb on + strand at 4466.337 kb on + strand at 4466.337 kb on + strand at 4466.337 kb on + strand at 4466.338 kb on - strand at 4466.338 kb on - strand at 4466.465 kb on + strand, within Psyr_3748 at 4466.465 kb on + strand, within Psyr_3748 at 4466.465 kb on + strand, within Psyr_3748 at 4466.465 kb on + strand, within Psyr_3748 at 4466.465 kb on + strand, within Psyr_3748 at 4466.466 kb on - strand, within Psyr_3748 at 4466.466 kb on - strand, within Psyr_3748 at 4466.466 kb on - strand, within Psyr_3748 at 4466.708 kb on + strand, within Psyr_3748 at 4466.708 kb on + strand, within Psyr_3748 at 4466.906 kb on + strand, within Psyr_3748 at 4466.907 kb on - strand, within Psyr_3748 at 4466.907 kb on - strand, within Psyr_3748 at 4466.907 kb on - strand, within Psyr_3748 at 4466.907 kb on - strand, within Psyr_3748 at 4466.955 kb on - strand, within Psyr_3748 at 4467.149 kb on + strand, within Psyr_3748 at 4467.149 kb on + strand, within Psyr_3748 at 4467.150 kb on - strand, within Psyr_3748 at 4467.678 kb on - strand, within Psyr_3748 at 4467.737 kb on - strand, within Psyr_3748 at 4467.831 kb on + strand at 4467.872 kb on + strand at 4467.872 kb on + strand at 4467.901 kb on - strand at 4467.979 kb on + strand at 4467.979 kb on + strand at 4467.979 kb on + strand at 4467.979 kb on + strand at 4467.980 kb on - strand at 4467.980 kb on - strand at 4468.053 kb on + strand at 4468.053 kb on + strand at 4468.054 kb on - strand at 4468.079 kb on + strand at 4468.079 kb on + strand at 4468.101 kb on + strand at 4468.101 kb on + strand at 4468.102 kb on - strand at 4468.102 kb on - strand at 4468.163 kb on + strand at 4468.163 kb on + strand at 4468.163 kb on + strand at 4468.164 kb on - strand at 4468.164 kb on - strand at 4468.164 kb on - strand at 4468.164 kb on - strand at 4468.323 kb on - strand, within Psyr_3749 at 4468.524 kb on + strand, within Psyr_3749 at 4468.524 kb on + strand, within Psyr_3749 at 4468.881 kb on + strand, within Psyr_3749 at 4468.962 kb on + strand, within Psyr_3749 at 4468.962 kb on + strand, within Psyr_3749 at 4468.962 kb on + strand, within Psyr_3749 at 4468.965 kb on + strand, within Psyr_3749 at 4468.965 kb on + strand, within Psyr_3749 at 4468.965 kb on + strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 4,465,520 + Psyr_3747 0.60 +0.7 4,465,520 + Psyr_3747 0.60 -0.6 4,465,521 - Psyr_3747 0.60 +0.8 4,465,560 + Psyr_3747 0.62 +1.1 4,465,599 - Psyr_3747 0.64 +0.6 4,465,682 + Psyr_3747 0.69 +0.6 4,465,682 + Psyr_3747 0.69 -0.2 4,465,682 + Psyr_3747 0.69 -0.4 4,466,019 - Psyr_3747 0.88 +0.4 4,466,062 + -1.4 4,466,063 - +3.3 4,466,258 + +0.7 4,466,258 + -0.6 4,466,258 + +0.0 4,466,258 + +1.0 4,466,259 - +0.6 4,466,259 - +0.1 4,466,260 + +0.0 4,466,260 + -2.7 4,466,294 + -0.6 4,466,295 - +0.2 4,466,295 - -1.0 4,466,337 + +0.0 4,466,337 + -0.8 4,466,337 + -1.8 4,466,337 + +0.7 4,466,337 + +0.9 4,466,338 - -0.8 4,466,338 - -0.4 4,466,465 + Psyr_3748 0.13 +3.6 4,466,465 + Psyr_3748 0.13 +0.8 4,466,465 + Psyr_3748 0.13 +1.0 4,466,465 + Psyr_3748 0.13 -0.6 4,466,465 + Psyr_3748 0.13 +0.0 4,466,466 - Psyr_3748 0.13 +1.3 4,466,466 - Psyr_3748 0.13 +0.9 4,466,466 - Psyr_3748 0.13 -0.1 4,466,708 + Psyr_3748 0.27 +0.4 4,466,708 + Psyr_3748 0.27 -0.9 4,466,906 + Psyr_3748 0.38 +0.2 4,466,907 - Psyr_3748 0.38 -0.6 4,466,907 - Psyr_3748 0.38 +2.1 4,466,907 - Psyr_3748 0.38 +1.2 4,466,907 - Psyr_3748 0.38 +0.0 4,466,955 - Psyr_3748 0.41 -0.9 4,467,149 + Psyr_3748 0.52 +0.2 4,467,149 + Psyr_3748 0.52 +0.9 4,467,150 - Psyr_3748 0.52 -0.3 4,467,678 - Psyr_3748 0.82 +0.9 4,467,737 - Psyr_3748 0.86 +0.8 4,467,831 + +2.6 4,467,872 + +0.4 4,467,872 + -0.7 4,467,901 - +0.4 4,467,979 + -1.7 4,467,979 + +2.6 4,467,979 + +0.3 4,467,979 + +0.6 4,467,980 - +0.8 4,467,980 - +1.6 4,468,053 + -2.4 4,468,053 + -0.6 4,468,054 - +1.3 4,468,079 + -0.8 4,468,079 + +2.4 4,468,101 + -0.6 4,468,101 + +0.6 4,468,102 - +1.4 4,468,102 - +0.3 4,468,163 + +0.3 4,468,163 + +0.3 4,468,163 + +0.4 4,468,164 - +1.0 4,468,164 - +0.3 4,468,164 - +0.7 4,468,164 - +1.7 4,468,323 - Psyr_3749 0.22 -0.4 4,468,524 + Psyr_3749 0.38 -0.4 4,468,524 + Psyr_3749 0.38 +1.1 4,468,881 + Psyr_3749 0.68 +0.2 4,468,962 + Psyr_3749 0.75 +0.4 4,468,962 + Psyr_3749 0.75 -0.8 4,468,962 + Psyr_3749 0.75 -1.2 4,468,965 + Psyr_3749 0.75 +0.6 4,468,965 + Psyr_3749 0.75 +1.0 4,468,965 + Psyr_3749 0.75 -0.9 4,468,966 - Psyr_3749 0.75 -0.8 4,468,966 - Psyr_3749 0.75 -0.9 4,468,966 - Psyr_3749 0.75 +0.0 4,468,966 - Psyr_3749 0.75 +0.4 4,468,966 - Psyr_3749 0.75 -2.8 4,468,966 - Psyr_3749 0.75 -1.4 4,468,966 - Psyr_3749 0.75 -1.0
Or see this region's nucleotide sequence