Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3317 and Psyr_3318 are separated by 301 nucleotides Psyr_3318 and Psyr_3319 are separated by 159 nucleotides Psyr_3319 and Psyr_3320 are separated by 126 nucleotides
Psyr_3317: Psyr_3317 - Integrase, catalytic region, at 3,968,791 to 3,969,597
_3317
Psyr_3318: Psyr_3318 - conserved hypothetical protein, at 3,969,899 to 3,971,116
_3318
Psyr_3319: Psyr_3319 - ThiJ/PfpI, at 3,971,276 to 3,971,959
_3319
Psyr_3320: Psyr_3320 - conserved hypothetical protein, at 3,972,086 to 3,972,235
_3320
Position (kb)
3969
3970
3971
3972 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3969.826 kb on + strand at 3969.838 kb on - strand at 3969.868 kb on + strand at 3969.869 kb on - strand at 3969.869 kb on - strand at 3969.878 kb on - strand at 3969.882 kb on - strand at 3969.882 kb on - strand at 3969.882 kb on - strand at 3969.882 kb on - strand at 3969.968 kb on + strand at 3970.066 kb on + strand, within Psyr_3318 at 3970.067 kb on - strand, within Psyr_3318 at 3970.067 kb on - strand, within Psyr_3318 at 3970.566 kb on - strand, within Psyr_3318 at 3970.566 kb on - strand, within Psyr_3318 at 3970.694 kb on + strand, within Psyr_3318 at 3970.715 kb on + strand, within Psyr_3318 at 3970.730 kb on + strand, within Psyr_3318 at 3970.730 kb on + strand, within Psyr_3318 at 3970.823 kb on - strand, within Psyr_3318 at 3970.916 kb on + strand, within Psyr_3318 at 3970.916 kb on + strand, within Psyr_3318 at 3970.916 kb on + strand, within Psyr_3318 at 3970.916 kb on + strand, within Psyr_3318 at 3970.917 kb on - strand, within Psyr_3318 at 3970.917 kb on - strand, within Psyr_3318 at 3970.994 kb on + strand, within Psyr_3318 at 3970.995 kb on - strand at 3971.247 kb on + strand at 3971.375 kb on + strand, within Psyr_3319 at 3971.375 kb on + strand, within Psyr_3319 at 3971.376 kb on - strand, within Psyr_3319 at 3971.376 kb on - strand, within Psyr_3319 at 3971.694 kb on + strand, within Psyr_3319 at 3971.800 kb on - strand, within Psyr_3319 at 3971.844 kb on - strand, within Psyr_3319 at 3971.870 kb on + strand, within Psyr_3319 at 3971.871 kb on - strand, within Psyr_3319 at 3971.871 kb on - strand, within Psyr_3319 at 3971.874 kb on + strand, within Psyr_3319 at 3971.875 kb on - strand, within Psyr_3319 at 3972.020 kb on + strand at 3972.020 kb on + strand at 3972.020 kb on + strand at 3972.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 3,969,826 + +1.4 3,969,838 - +0.0 3,969,868 + +0.1 3,969,869 - +1.3 3,969,869 - -0.5 3,969,878 - +1.3 3,969,882 - +0.6 3,969,882 - +1.2 3,969,882 - -0.6 3,969,882 - +1.1 3,969,968 + +0.8 3,970,066 + Psyr_3318 0.14 +0.1 3,970,067 - Psyr_3318 0.14 +0.3 3,970,067 - Psyr_3318 0.14 +0.1 3,970,566 - Psyr_3318 0.55 +1.6 3,970,566 - Psyr_3318 0.55 -0.5 3,970,694 + Psyr_3318 0.65 -0.4 3,970,715 + Psyr_3318 0.67 +0.8 3,970,730 + Psyr_3318 0.68 +0.8 3,970,730 + Psyr_3318 0.68 +1.0 3,970,823 - Psyr_3318 0.76 +0.6 3,970,916 + Psyr_3318 0.83 +1.1 3,970,916 + Psyr_3318 0.83 +0.8 3,970,916 + Psyr_3318 0.83 +0.1 3,970,916 + Psyr_3318 0.83 +2.1 3,970,917 - Psyr_3318 0.84 +2.9 3,970,917 - Psyr_3318 0.84 -0.3 3,970,994 + Psyr_3318 0.90 +0.6 3,970,995 - +0.8 3,971,247 + +1.8 3,971,375 + Psyr_3319 0.14 +0.9 3,971,375 + Psyr_3319 0.14 -1.8 3,971,376 - Psyr_3319 0.15 +0.7 3,971,376 - Psyr_3319 0.15 +0.0 3,971,694 + Psyr_3319 0.61 +0.2 3,971,800 - Psyr_3319 0.77 -2.3 3,971,844 - Psyr_3319 0.83 +0.5 3,971,870 + Psyr_3319 0.87 +0.2 3,971,871 - Psyr_3319 0.87 +0.2 3,971,871 - Psyr_3319 0.87 +0.9 3,971,874 + Psyr_3319 0.87 +2.5 3,971,875 - Psyr_3319 0.88 +0.4 3,972,020 + +0.9 3,972,020 + -1.0 3,972,020 + -0.0 3,972,021 - -1.7
Or see this region's nucleotide sequence