Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3217 and Psyr_3218 are separated by 166 nucleotides Psyr_3218 and Psyr_3219 are separated by 24 nucleotides Psyr_3219 and Psyr_3220 are separated by 57 nucleotides
Psyr_3217: Psyr_3217 - Protein of unknown function DUF6, at 3,853,334 to 3,854,221
_3217
Psyr_3218: Psyr_3218 - hypothetical protein, at 3,854,388 to 3,854,702
_3218
Psyr_3219: Psyr_3219 - Peptidase M24, at 3,854,727 to 3,856,535
_3219
Psyr_3220: Psyr_3220 - Aminotransferase, class V, at 3,856,593 to 3,857,783
_3220
Position (kb)
3854
3855
3856
3857 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3853.823 kb on + strand, within Psyr_3217 at 3853.823 kb on + strand, within Psyr_3217 at 3853.906 kb on - strand, within Psyr_3217 at 3853.923 kb on + strand, within Psyr_3217 at 3853.923 kb on + strand, within Psyr_3217 at 3853.959 kb on + strand, within Psyr_3217 at 3854.005 kb on + strand, within Psyr_3217 at 3854.045 kb on - strand, within Psyr_3217 at 3854.045 kb on - strand, within Psyr_3217 at 3854.225 kb on + strand at 3854.226 kb on - strand at 3854.254 kb on - strand at 3854.284 kb on + strand at 3854.297 kb on + strand at 3854.377 kb on + strand at 3854.395 kb on - strand at 3854.417 kb on - strand at 3854.521 kb on - strand, within Psyr_3218 at 3854.604 kb on + strand, within Psyr_3218 at 3854.631 kb on + strand, within Psyr_3218 at 3854.701 kb on - strand at 3854.701 kb on - strand at 3854.812 kb on + strand at 3854.813 kb on - strand at 3854.987 kb on - strand, within Psyr_3219 at 3854.990 kb on + strand, within Psyr_3219 at 3854.990 kb on + strand, within Psyr_3219 at 3854.991 kb on - strand, within Psyr_3219 at 3854.991 kb on - strand, within Psyr_3219 at 3855.055 kb on + strand, within Psyr_3219 at 3855.094 kb on + strand, within Psyr_3219 at 3855.124 kb on + strand, within Psyr_3219 at 3855.146 kb on + strand, within Psyr_3219 at 3855.240 kb on - strand, within Psyr_3219 at 3855.313 kb on + strand, within Psyr_3219 at 3855.314 kb on - strand, within Psyr_3219 at 3855.354 kb on + strand, within Psyr_3219 at 3855.354 kb on + strand, within Psyr_3219 at 3855.354 kb on + strand, within Psyr_3219 at 3855.354 kb on + strand, within Psyr_3219 at 3855.593 kb on + strand, within Psyr_3219 at 3855.622 kb on - strand, within Psyr_3219 at 3855.670 kb on + strand, within Psyr_3219 at 3855.760 kb on + strand, within Psyr_3219 at 3855.760 kb on + strand, within Psyr_3219 at 3855.760 kb on + strand, within Psyr_3219 at 3855.760 kb on + strand, within Psyr_3219 at 3856.039 kb on + strand, within Psyr_3219 at 3856.126 kb on + strand, within Psyr_3219 at 3856.321 kb on - strand, within Psyr_3219 at 3856.411 kb on + strand at 3856.448 kb on - strand at 3856.582 kb on + strand at 3856.665 kb on + strand at 3856.665 kb on + strand at 3856.665 kb on + strand at 3856.666 kb on - strand at 3856.666 kb on - strand at 3856.666 kb on - strand at 3856.666 kb on - strand at 3856.704 kb on + strand at 3856.844 kb on + strand, within Psyr_3220 at 3856.851 kb on - strand, within Psyr_3220 at 3856.851 kb on - strand, within Psyr_3220 at 3856.895 kb on + strand, within Psyr_3220 at 3856.895 kb on + strand, within Psyr_3220 at 3856.896 kb on - strand, within Psyr_3220 at 3856.896 kb on - strand, within Psyr_3220 at 3856.896 kb on - strand, within Psyr_3220 at 3856.896 kb on - strand, within Psyr_3220 at 3856.951 kb on - strand, within Psyr_3220 at 3857.110 kb on + strand, within Psyr_3220 at 3857.110 kb on + strand, within Psyr_3220 at 3857.110 kb on + strand, within Psyr_3220 at 3857.110 kb on + strand, within Psyr_3220 at 3857.110 kb on + strand, within Psyr_3220 at 3857.110 kb on + strand, within Psyr_3220 at 3857.110 kb on + strand, within Psyr_3220 at 3857.110 kb on + strand, within Psyr_3220 at 3857.110 kb on + strand, within Psyr_3220 at 3857.111 kb on - strand, within Psyr_3220 at 3857.111 kb on - strand, within Psyr_3220 at 3857.111 kb on - strand, within Psyr_3220 at 3857.111 kb on - strand, within Psyr_3220 at 3857.111 kb on - strand, within Psyr_3220 at 3857.111 kb on - strand, within Psyr_3220 at 3857.111 kb on - strand, within Psyr_3220 at 3857.137 kb on + strand, within Psyr_3220 at 3857.250 kb on + strand, within Psyr_3220 at 3857.251 kb on - strand, within Psyr_3220 at 3857.471 kb on + strand, within Psyr_3220 at 3857.471 kb on + strand, within Psyr_3220 at 3857.472 kb on - strand, within Psyr_3220
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 3,853,823 + Psyr_3217 0.55 +1.6 3,853,823 + Psyr_3217 0.55 -0.7 3,853,906 - Psyr_3217 0.64 +1.9 3,853,923 + Psyr_3217 0.66 -0.0 3,853,923 + Psyr_3217 0.66 -1.2 3,853,959 + Psyr_3217 0.70 +0.2 3,854,005 + Psyr_3217 0.76 +1.6 3,854,045 - Psyr_3217 0.80 +0.1 3,854,045 - Psyr_3217 0.80 +1.4 3,854,225 + +1.1 3,854,226 - -0.7 3,854,254 - +0.9 3,854,284 + +0.2 3,854,297 + +0.7 3,854,377 + +0.0 3,854,395 - +0.3 3,854,417 - +0.8 3,854,521 - Psyr_3218 0.42 +2.3 3,854,604 + Psyr_3218 0.69 -1.4 3,854,631 + Psyr_3218 0.77 +0.5 3,854,701 - -1.5 3,854,701 - -0.2 3,854,812 + -0.6 3,854,813 - -1.2 3,854,987 - Psyr_3219 0.14 +0.9 3,854,990 + Psyr_3219 0.15 -0.1 3,854,990 + Psyr_3219 0.15 +1.1 3,854,991 - Psyr_3219 0.15 +0.8 3,854,991 - Psyr_3219 0.15 -0.2 3,855,055 + Psyr_3219 0.18 -1.6 3,855,094 + Psyr_3219 0.20 +0.2 3,855,124 + Psyr_3219 0.22 +1.2 3,855,146 + Psyr_3219 0.23 -0.9 3,855,240 - Psyr_3219 0.28 +0.2 3,855,313 + Psyr_3219 0.32 +0.4 3,855,314 - Psyr_3219 0.32 +0.8 3,855,354 + Psyr_3219 0.35 +0.2 3,855,354 + Psyr_3219 0.35 +2.3 3,855,354 + Psyr_3219 0.35 +0.2 3,855,354 + Psyr_3219 0.35 -0.3 3,855,593 + Psyr_3219 0.48 +4.1 3,855,622 - Psyr_3219 0.49 -1.0 3,855,670 + Psyr_3219 0.52 +2.3 3,855,760 + Psyr_3219 0.57 +0.6 3,855,760 + Psyr_3219 0.57 +0.8 3,855,760 + Psyr_3219 0.57 +3.5 3,855,760 + Psyr_3219 0.57 +0.9 3,856,039 + Psyr_3219 0.73 +1.6 3,856,126 + Psyr_3219 0.77 +0.7 3,856,321 - Psyr_3219 0.88 -2.2 3,856,411 + -0.5 3,856,448 - -0.8 3,856,582 + -0.4 3,856,665 + -0.2 3,856,665 + -0.4 3,856,665 + +0.9 3,856,666 - -0.2 3,856,666 - -1.8 3,856,666 - -1.1 3,856,666 - +0.1 3,856,704 + +0.3 3,856,844 + Psyr_3220 0.21 -0.4 3,856,851 - Psyr_3220 0.22 +0.0 3,856,851 - Psyr_3220 0.22 -1.3 3,856,895 + Psyr_3220 0.25 +0.0 3,856,895 + Psyr_3220 0.25 +4.4 3,856,896 - Psyr_3220 0.25 -0.4 3,856,896 - Psyr_3220 0.25 -0.2 3,856,896 - Psyr_3220 0.25 +0.5 3,856,896 - Psyr_3220 0.25 +2.2 3,856,951 - Psyr_3220 0.30 +1.4 3,857,110 + Psyr_3220 0.43 +1.9 3,857,110 + Psyr_3220 0.43 +0.5 3,857,110 + Psyr_3220 0.43 -0.4 3,857,110 + Psyr_3220 0.43 -2.0 3,857,110 + Psyr_3220 0.43 +0.0 3,857,110 + Psyr_3220 0.43 +1.1 3,857,110 + Psyr_3220 0.43 +1.6 3,857,110 + Psyr_3220 0.43 -1.1 3,857,110 + Psyr_3220 0.43 -0.2 3,857,111 - Psyr_3220 0.43 +0.5 3,857,111 - Psyr_3220 0.43 -1.5 3,857,111 - Psyr_3220 0.43 +0.3 3,857,111 - Psyr_3220 0.43 -0.7 3,857,111 - Psyr_3220 0.43 -0.0 3,857,111 - Psyr_3220 0.43 -0.2 3,857,111 - Psyr_3220 0.43 +0.4 3,857,137 + Psyr_3220 0.46 +2.9 3,857,250 + Psyr_3220 0.55 +0.3 3,857,251 - Psyr_3220 0.55 -0.0 3,857,471 + Psyr_3220 0.74 -0.8 3,857,471 + Psyr_3220 0.74 -0.5 3,857,472 - Psyr_3220 0.74 -0.1
Or see this region's nucleotide sequence