Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2732 and Psyr_2733 are separated by 84 nucleotides Psyr_2733 and Psyr_2734 are separated by 86 nucleotides Psyr_2734 and Psyr_2735 are separated by 277 nucleotides
Psyr_2732: Psyr_2732 - conserved hypothetical protein; putative signal peptide, at 3,316,494 to 3,317,441
_2732
Psyr_2733: Psyr_2733 - Short-chain dehydrogenase/reductase SDR, at 3,317,526 to 3,318,293
_2733
Psyr_2734: Psyr_2734 - benzaldehyde dehydrogenase (NAD+), at 3,318,380 to 3,319,861
_2734
Psyr_2735: Psyr_2735 - Helix-turn-helix, Fis-type, at 3,320,139 to 3,321,902
_2735
Position (kb)
3318
3319
3320 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3317.407 kb on - strand at 3317.455 kb on + strand at 3317.455 kb on + strand at 3317.455 kb on + strand at 3317.459 kb on + strand at 3317.459 kb on + strand at 3317.459 kb on + strand at 3317.459 kb on + strand at 3317.459 kb on + strand at 3317.459 kb on + strand at 3317.459 kb on + strand at 3317.459 kb on + strand at 3317.460 kb on - strand at 3317.460 kb on - strand at 3317.527 kb on + strand at 3317.533 kb on + strand at 3317.533 kb on + strand at 3317.533 kb on + strand at 3317.533 kb on + strand at 3317.534 kb on - strand at 3317.534 kb on - strand at 3317.534 kb on - strand at 3317.534 kb on - strand at 3317.534 kb on - strand at 3317.570 kb on + strand at 3317.818 kb on + strand, within Psyr_2733 at 3317.819 kb on - strand, within Psyr_2733 at 3317.877 kb on - strand, within Psyr_2733 at 3317.878 kb on + strand, within Psyr_2733 at 3317.878 kb on + strand, within Psyr_2733 at 3317.879 kb on - strand, within Psyr_2733 at 3317.960 kb on + strand, within Psyr_2733 at 3318.293 kb on + strand at 3318.294 kb on - strand at 3318.307 kb on + strand at 3318.307 kb on + strand at 3318.307 kb on + strand at 3318.307 kb on + strand at 3318.308 kb on - strand at 3318.308 kb on - strand at 3318.308 kb on - strand at 3318.369 kb on - strand at 3318.435 kb on + strand at 3318.435 kb on + strand at 3318.436 kb on - strand at 3318.768 kb on + strand, within Psyr_2734 at 3318.768 kb on + strand, within Psyr_2734 at 3319.455 kb on - strand, within Psyr_2734 at 3319.572 kb on + strand, within Psyr_2734 at 3319.572 kb on + strand, within Psyr_2734 at 3319.572 kb on + strand, within Psyr_2734 at 3319.572 kb on + strand, within Psyr_2734 at 3319.573 kb on - strand, within Psyr_2734 at 3319.573 kb on - strand, within Psyr_2734 at 3319.573 kb on - strand, within Psyr_2734 at 3319.573 kb on - strand, within Psyr_2734 at 3319.573 kb on - strand, within Psyr_2734 at 3319.573 kb on - strand, within Psyr_2734 at 3319.573 kb on - strand, within Psyr_2734 at 3319.625 kb on + strand, within Psyr_2734 at 3319.625 kb on + strand, within Psyr_2734 at 3319.625 kb on + strand, within Psyr_2734 at 3319.625 kb on + strand, within Psyr_2734 at 3319.626 kb on - strand, within Psyr_2734 at 3319.626 kb on - strand, within Psyr_2734 at 3319.861 kb on + strand at 3319.861 kb on + strand at 3319.861 kb on + strand at 3319.862 kb on - strand at 3319.862 kb on - strand at 3319.862 kb on - strand at 3319.864 kb on + strand at 3319.864 kb on + strand at 3319.865 kb on - strand at 3319.865 kb on - strand at 3319.877 kb on + strand at 3319.877 kb on + strand at 3319.877 kb on + strand at 3319.927 kb on + strand at 3319.927 kb on + strand at 3319.928 kb on - strand at 3319.957 kb on + strand at 3320.078 kb on + strand at 3320.078 kb on + strand at 3320.079 kb on - strand at 3320.084 kb on + strand at 3320.133 kb on - strand at 3320.324 kb on + strand, within Psyr_2735 at 3320.324 kb on + strand, within Psyr_2735 at 3320.324 kb on + strand, within Psyr_2735 at 3320.324 kb on + strand, within Psyr_2735 at 3320.324 kb on + strand, within Psyr_2735 at 3320.325 kb on - strand, within Psyr_2735 at 3320.325 kb on - strand, within Psyr_2735 at 3320.325 kb on - strand, within Psyr_2735 at 3320.325 kb on - strand, within Psyr_2735 at 3320.325 kb on - strand, within Psyr_2735 at 3320.325 kb on - strand, within Psyr_2735 at 3320.325 kb on - strand, within Psyr_2735 at 3320.325 kb on - strand, within Psyr_2735 at 3320.325 kb on - strand, within Psyr_2735 at 3320.565 kb on + strand, within Psyr_2735 at 3320.565 kb on + strand, within Psyr_2735 at 3320.623 kb on - strand, within Psyr_2735 at 3320.623 kb on - strand, within Psyr_2735 at 3320.623 kb on - strand, within Psyr_2735 at 3320.805 kb on + strand, within Psyr_2735 at 3320.837 kb on - strand, within Psyr_2735
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 3,317,407 - -0.0 3,317,455 + -0.6 3,317,455 + +0.2 3,317,455 + -0.8 3,317,459 + -0.1 3,317,459 + +1.0 3,317,459 + -1.4 3,317,459 + -1.4 3,317,459 + +0.6 3,317,459 + -1.7 3,317,459 + -1.7 3,317,459 + +0.1 3,317,460 - +0.1 3,317,460 - -1.0 3,317,527 + -0.4 3,317,533 + -0.3 3,317,533 + +1.1 3,317,533 + -1.4 3,317,533 + -0.4 3,317,534 - -0.5 3,317,534 - -1.9 3,317,534 - -0.0 3,317,534 - -0.3 3,317,534 - -0.2 3,317,570 + -0.2 3,317,818 + Psyr_2733 0.38 -1.8 3,317,819 - Psyr_2733 0.38 -0.6 3,317,877 - Psyr_2733 0.46 +0.3 3,317,878 + Psyr_2733 0.46 +0.1 3,317,878 + Psyr_2733 0.46 -2.3 3,317,879 - Psyr_2733 0.46 -0.5 3,317,960 + Psyr_2733 0.57 +0.7 3,318,293 + +0.6 3,318,294 - -1.4 3,318,307 + +0.1 3,318,307 + +0.5 3,318,307 + +0.1 3,318,307 + +1.5 3,318,308 - -0.9 3,318,308 - -0.5 3,318,308 - -0.1 3,318,369 - -2.9 3,318,435 + -0.3 3,318,435 + +0.4 3,318,436 - +0.9 3,318,768 + Psyr_2734 0.26 +2.6 3,318,768 + Psyr_2734 0.26 +1.3 3,319,455 - Psyr_2734 0.73 +0.2 3,319,572 + Psyr_2734 0.80 +1.1 3,319,572 + Psyr_2734 0.80 -2.0 3,319,572 + Psyr_2734 0.80 -0.2 3,319,572 + Psyr_2734 0.80 +1.6 3,319,573 - Psyr_2734 0.80 +3.1 3,319,573 - Psyr_2734 0.80 +1.1 3,319,573 - Psyr_2734 0.80 +3.1 3,319,573 - Psyr_2734 0.80 +0.4 3,319,573 - Psyr_2734 0.80 -1.5 3,319,573 - Psyr_2734 0.80 +0.3 3,319,573 - Psyr_2734 0.80 +0.1 3,319,625 + Psyr_2734 0.84 +0.9 3,319,625 + Psyr_2734 0.84 +0.8 3,319,625 + Psyr_2734 0.84 -0.7 3,319,625 + Psyr_2734 0.84 -0.0 3,319,626 - Psyr_2734 0.84 +0.6 3,319,626 - Psyr_2734 0.84 +0.7 3,319,861 + -0.8 3,319,861 + -0.4 3,319,861 + +0.6 3,319,862 - -0.4 3,319,862 - +3.3 3,319,862 - +1.1 3,319,864 + +0.8 3,319,864 + +1.2 3,319,865 - +0.9 3,319,865 - -4.0 3,319,877 + -0.7 3,319,877 + -0.6 3,319,877 + +1.0 3,319,927 + -0.6 3,319,927 + -0.7 3,319,928 - +1.3 3,319,957 + +0.9 3,320,078 + -3.3 3,320,078 + -0.3 3,320,079 - -0.0 3,320,084 + +0.8 3,320,133 - +0.9 3,320,324 + Psyr_2735 0.10 -1.4 3,320,324 + Psyr_2735 0.10 -0.9 3,320,324 + Psyr_2735 0.10 +0.4 3,320,324 + Psyr_2735 0.10 +2.1 3,320,324 + Psyr_2735 0.10 +0.3 3,320,325 - Psyr_2735 0.11 -3.6 3,320,325 - Psyr_2735 0.11 +0.1 3,320,325 - Psyr_2735 0.11 -0.4 3,320,325 - Psyr_2735 0.11 +1.1 3,320,325 - Psyr_2735 0.11 +2.7 3,320,325 - Psyr_2735 0.11 -0.3 3,320,325 - Psyr_2735 0.11 +0.3 3,320,325 - Psyr_2735 0.11 -0.3 3,320,325 - Psyr_2735 0.11 -2.0 3,320,565 + Psyr_2735 0.24 -1.3 3,320,565 + Psyr_2735 0.24 +0.8 3,320,623 - Psyr_2735 0.27 -0.9 3,320,623 - Psyr_2735 0.27 -0.0 3,320,623 - Psyr_2735 0.27 -0.9 3,320,805 + Psyr_2735 0.38 -0.4 3,320,837 - Psyr_2735 0.40 +2.6
Or see this region's nucleotide sequence