Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1433 and Psyr_1434 are separated by 572 nucleotides Psyr_1434 and Psyr_1435 are separated by 146 nucleotides
Psyr_1433: Psyr_1433 - C-5 cytosine-specific DNA methylase, at 1,621,535 to 1,622,950
_1433
Psyr_1434: Psyr_1434 - Antibiotic biosynthesis monooxygenase, at 1,623,523 to 1,623,834
_1434
Psyr_1435: Psyr_1435 - transcriptional regulator, LysR family, at 1,623,981 to 1,624,916
_1435
Position (kb) 1623
1624 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1622.543 kb on + strand, within Psyr_1433 at 1622.693 kb on + strand, within Psyr_1433 at 1622.847 kb on - strand at 1622.867 kb on + strand at 1622.867 kb on + strand at 1622.868 kb on - strand at 1622.890 kb on + strand at 1622.890 kb on + strand at 1622.890 kb on + strand at 1622.890 kb on + strand at 1622.890 kb on + strand at 1622.890 kb on + strand at 1622.891 kb on - strand at 1622.891 kb on - strand at 1622.891 kb on - strand at 1622.891 kb on - strand at 1622.944 kb on + strand at 1622.945 kb on - strand at 1622.945 kb on - strand at 1622.945 kb on - strand at 1622.945 kb on - strand at 1622.945 kb on - strand at 1622.945 kb on - strand at 1622.952 kb on + strand at 1622.952 kb on + strand at 1622.952 kb on + strand at 1622.953 kb on - strand at 1622.953 kb on - strand at 1622.953 kb on - strand at 1622.953 kb on - strand at 1622.953 kb on - strand at 1622.953 kb on - strand at 1623.001 kb on + strand at 1623.002 kb on - strand at 1623.090 kb on + strand at 1623.090 kb on + strand at 1623.108 kb on + strand at 1623.108 kb on + strand at 1623.108 kb on + strand at 1623.111 kb on + strand at 1623.111 kb on + strand at 1623.111 kb on + strand at 1623.111 kb on + strand at 1623.111 kb on + strand at 1623.111 kb on + strand at 1623.111 kb on + strand at 1623.111 kb on + strand at 1623.111 kb on + strand at 1623.111 kb on + strand at 1623.111 kb on + strand at 1623.112 kb on - strand at 1623.112 kb on - strand at 1623.112 kb on - strand at 1623.112 kb on - strand at 1623.115 kb on + strand at 1623.115 kb on + strand at 1623.116 kb on - strand at 1623.145 kb on + strand at 1623.145 kb on + strand at 1623.145 kb on + strand at 1623.146 kb on - strand at 1623.146 kb on - strand at 1623.146 kb on - strand at 1623.161 kb on + strand at 1623.162 kb on - strand at 1623.225 kb on + strand at 1623.225 kb on + strand at 1623.225 kb on + strand at 1623.226 kb on - strand at 1623.226 kb on - strand at 1623.229 kb on + strand at 1623.229 kb on + strand at 1623.229 kb on + strand at 1623.230 kb on - strand at 1623.230 kb on - strand at 1623.231 kb on + strand at 1623.231 kb on + strand at 1623.231 kb on + strand at 1623.231 kb on + strand at 1623.231 kb on + strand at 1623.231 kb on + strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.232 kb on - strand at 1623.269 kb on + strand at 1623.269 kb on + strand at 1623.270 kb on - strand at 1623.270 kb on - strand at 1623.270 kb on - strand at 1623.278 kb on + strand at 1623.279 kb on - strand at 1623.298 kb on + strand at 1623.298 kb on + strand at 1623.298 kb on + strand at 1623.298 kb on + strand at 1623.299 kb on - strand at 1623.314 kb on + strand at 1623.315 kb on - strand at 1623.324 kb on - strand at 1623.331 kb on + strand at 1623.361 kb on + strand at 1623.377 kb on + strand at 1623.377 kb on + strand at 1623.377 kb on + strand at 1623.377 kb on + strand at 1623.377 kb on + strand at 1623.377 kb on + strand at 1623.378 kb on - strand at 1623.378 kb on - strand at 1623.378 kb on - strand at 1623.378 kb on - strand at 1623.378 kb on - strand at 1623.378 kb on - strand at 1623.387 kb on + strand at 1623.387 kb on + strand at 1623.388 kb on - strand at 1623.388 kb on - strand at 1623.388 kb on - strand at 1623.427 kb on + strand at 1623.439 kb on + strand at 1623.439 kb on + strand at 1623.439 kb on + strand at 1623.440 kb on - strand at 1623.440 kb on - strand at 1623.472 kb on + strand at 1623.472 kb on + strand at 1623.472 kb on + strand at 1623.473 kb on - strand at 1623.473 kb on - strand at 1623.483 kb on + strand at 1623.483 kb on + strand at 1623.484 kb on - strand at 1623.484 kb on - strand at 1623.547 kb on + strand at 1623.547 kb on + strand at 1623.623 kb on + strand, within Psyr_1434 at 1623.623 kb on + strand, within Psyr_1434 at 1623.624 kb on - strand, within Psyr_1434 at 1623.719 kb on + strand, within Psyr_1434 at 1623.720 kb on - strand, within Psyr_1434 at 1623.733 kb on - strand, within Psyr_1434 at 1623.825 kb on + strand at 1623.825 kb on + strand at 1623.825 kb on + strand at 1623.826 kb on - strand at 1623.826 kb on - strand at 1623.826 kb on - strand at 1623.826 kb on - strand at 1623.827 kb on + strand at 1623.827 kb on + strand at 1623.827 kb on + strand at 1623.827 kb on + strand at 1623.827 kb on + strand at 1623.827 kb on + strand at 1623.827 kb on + strand at 1623.827 kb on + strand at 1623.827 kb on + strand at 1623.828 kb on - strand at 1623.828 kb on - strand at 1623.828 kb on - strand at 1623.828 kb on - strand at 1623.828 kb on - strand at 1623.828 kb on - strand at 1623.828 kb on - strand at 1623.847 kb on + strand at 1623.847 kb on + strand at 1623.848 kb on - strand at 1623.848 kb on - strand at 1623.848 kb on - strand at 1623.848 kb on - strand at 1623.848 kb on - strand at 1623.851 kb on + strand at 1623.851 kb on + strand at 1623.851 kb on + strand at 1623.852 kb on - strand at 1623.852 kb on - strand at 1623.852 kb on - strand at 1623.852 kb on - strand at 1623.852 kb on - strand at 1623.884 kb on + strand at 1623.885 kb on - strand at 1623.885 kb on - strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.969 kb on - strand at 1623.969 kb on - strand at 1624.047 kb on + strand at 1624.047 kb on + strand at 1624.211 kb on - strand, within Psyr_1435 at 1624.211 kb on - strand, within Psyr_1435 at 1624.273 kb on - strand, within Psyr_1435 at 1624.318 kb on + strand, within Psyr_1435 at 1624.318 kb on + strand, within Psyr_1435 at 1624.318 kb on + strand, within Psyr_1435 at 1624.339 kb on + strand, within Psyr_1435 at 1624.339 kb on + strand, within Psyr_1435 at 1624.339 kb on + strand, within Psyr_1435 at 1624.339 kb on + strand, within Psyr_1435 at 1624.339 kb on + strand, within Psyr_1435 at 1624.339 kb on + strand, within Psyr_1435 at 1624.339 kb on + strand, within Psyr_1435 at 1624.339 kb on + strand, within Psyr_1435 at 1624.339 kb on + strand, within Psyr_1435 at 1624.340 kb on - strand, within Psyr_1435 at 1624.340 kb on - strand, within Psyr_1435 at 1624.340 kb on - strand, within Psyr_1435 at 1624.340 kb on - strand, within Psyr_1435 at 1624.340 kb on - strand, within Psyr_1435 at 1624.340 kb on - strand, within Psyr_1435 at 1624.385 kb on + strand, within Psyr_1435 at 1624.386 kb on - strand, within Psyr_1435 at 1624.402 kb on + strand, within Psyr_1435 at 1624.402 kb on + strand, within Psyr_1435 at 1624.403 kb on - strand, within Psyr_1435 at 1624.455 kb on + strand, within Psyr_1435 at 1624.455 kb on + strand, within Psyr_1435 at 1624.455 kb on + strand, within Psyr_1435 at 1624.456 kb on - strand, within Psyr_1435 at 1624.456 kb on - strand, within Psyr_1435 at 1624.456 kb on - strand, within Psyr_1435 at 1624.456 kb on - strand, within Psyr_1435 at 1624.456 kb on - strand, within Psyr_1435 at 1624.459 kb on + strand, within Psyr_1435 at 1624.459 kb on + strand, within Psyr_1435 at 1624.459 kb on + strand, within Psyr_1435 at 1624.460 kb on - strand, within Psyr_1435 at 1624.460 kb on - strand, within Psyr_1435 at 1624.553 kb on + strand, within Psyr_1435 at 1624.553 kb on + strand, within Psyr_1435 at 1624.553 kb on + strand, within Psyr_1435 at 1624.553 kb on + strand, within Psyr_1435 at 1624.553 kb on + strand, within Psyr_1435 at 1624.553 kb on + strand, within Psyr_1435 at 1624.553 kb on + strand, within Psyr_1435 at 1624.554 kb on - strand, within Psyr_1435 at 1624.554 kb on - strand, within Psyr_1435 at 1624.554 kb on - strand, within Psyr_1435 at 1624.554 kb on - strand, within Psyr_1435 at 1624.723 kb on + strand, within Psyr_1435 at 1624.723 kb on + strand, within Psyr_1435 at 1624.724 kb on - strand, within Psyr_1435 at 1624.724 kb on - strand, within Psyr_1435 at 1624.724 kb on - strand, within Psyr_1435 at 1624.724 kb on - strand, within Psyr_1435 at 1624.754 kb on + strand, within Psyr_1435 at 1624.754 kb on + strand, within Psyr_1435 at 1624.754 kb on + strand, within Psyr_1435 at 1624.761 kb on - strand, within Psyr_1435 at 1624.807 kb on - strand, within Psyr_1435
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 1,622,543 + Psyr_1433 0.71 -1.4 1,622,693 + Psyr_1433 0.82 -0.9 1,622,847 - +1.1 1,622,867 + -1.9 1,622,867 + -0.7 1,622,868 - -1.5 1,622,890 + -2.1 1,622,890 + +1.1 1,622,890 + +1.1 1,622,890 + -0.5 1,622,890 + +0.1 1,622,890 + -0.6 1,622,891 - +0.5 1,622,891 - +0.5 1,622,891 - +1.5 1,622,891 - +0.1 1,622,944 + -0.6 1,622,945 - -0.6 1,622,945 - +2.6 1,622,945 - -0.2 1,622,945 - +0.7 1,622,945 - +0.0 1,622,945 - +0.8 1,622,952 + +0.1 1,622,952 + +0.5 1,622,952 + +1.0 1,622,953 - -1.0 1,622,953 - -0.6 1,622,953 - -0.5 1,622,953 - -1.4 1,622,953 - -0.3 1,622,953 - -0.8 1,623,001 + -1.3 1,623,002 - +0.5 1,623,090 + +0.3 1,623,090 + -1.4 1,623,108 + -0.9 1,623,108 + +0.1 1,623,108 + -0.7 1,623,111 + -0.7 1,623,111 + -2.0 1,623,111 + +0.6 1,623,111 + -1.0 1,623,111 + -0.5 1,623,111 + -0.9 1,623,111 + -1.0 1,623,111 + +0.2 1,623,111 + +0.7 1,623,111 + +1.6 1,623,111 + -2.3 1,623,112 - -0.7 1,623,112 - -0.0 1,623,112 - -1.4 1,623,112 - -0.3 1,623,115 + +0.2 1,623,115 + +0.4 1,623,116 - +1.1 1,623,145 + -1.3 1,623,145 + +0.4 1,623,145 + +0.1 1,623,146 - -0.8 1,623,146 - -1.5 1,623,146 - +0.2 1,623,161 + -0.3 1,623,162 - -0.9 1,623,225 + +0.8 1,623,225 + +0.1 1,623,225 + -0.1 1,623,226 - -3.4 1,623,226 - -0.6 1,623,229 + +0.9 1,623,229 + -0.4 1,623,229 + +0.6 1,623,230 - -0.5 1,623,230 - -1.4 1,623,231 + +0.7 1,623,231 + +0.4 1,623,231 + -1.2 1,623,231 + -1.3 1,623,231 + -2.0 1,623,231 + -0.8 1,623,232 - -0.7 1,623,232 - +1.3 1,623,232 - -1.6 1,623,232 - +0.4 1,623,232 - +0.4 1,623,232 - +0.4 1,623,232 - -2.5 1,623,232 - -0.7 1,623,232 - -1.0 1,623,232 - -0.4 1,623,232 - +0.5 1,623,232 - +0.3 1,623,269 + -0.7 1,623,269 + -1.4 1,623,270 - -1.2 1,623,270 - +0.4 1,623,270 - +0.4 1,623,278 + -1.3 1,623,279 - +0.4 1,623,298 + +1.0 1,623,298 + -0.9 1,623,298 + -0.7 1,623,298 + -0.5 1,623,299 - -0.9 1,623,314 + +0.9 1,623,315 - -0.5 1,623,324 - +0.6 1,623,331 + -0.8 1,623,361 + +1.1 1,623,377 + -0.5 1,623,377 + +0.0 1,623,377 + +0.5 1,623,377 + -0.4 1,623,377 + -1.1 1,623,377 + -0.5 1,623,378 - -1.9 1,623,378 - -0.1 1,623,378 - +0.6 1,623,378 - -1.5 1,623,378 - -0.2 1,623,378 - -0.2 1,623,387 + -0.3 1,623,387 + +0.4 1,623,388 - -0.5 1,623,388 - -0.7 1,623,388 - -0.5 1,623,427 + -0.5 1,623,439 + +3.1 1,623,439 + -0.5 1,623,439 + -2.3 1,623,440 - -0.1 1,623,440 - -0.4 1,623,472 + -1.1 1,623,472 + +0.8 1,623,472 + -0.8 1,623,473 - +1.1 1,623,473 - -0.5 1,623,483 + +0.4 1,623,483 + -0.2 1,623,484 - -0.6 1,623,484 - -0.4 1,623,547 + +0.7 1,623,547 + +0.3 1,623,623 + Psyr_1434 0.32 +0.2 1,623,623 + Psyr_1434 0.32 +0.8 1,623,624 - Psyr_1434 0.32 +4.0 1,623,719 + Psyr_1434 0.63 -1.0 1,623,720 - Psyr_1434 0.63 +0.7 1,623,733 - Psyr_1434 0.67 +0.7 1,623,825 + -1.5 1,623,825 + -0.1 1,623,825 + -1.3 1,623,826 - +0.0 1,623,826 - +0.3 1,623,826 - -3.0 1,623,826 - -2.2 1,623,827 + -1.5 1,623,827 + +1.1 1,623,827 + -0.1 1,623,827 + -1.0 1,623,827 + -0.1 1,623,827 + +1.1 1,623,827 + -0.6 1,623,827 + -0.8 1,623,827 + +2.1 1,623,828 - -1.0 1,623,828 - +2.2 1,623,828 - -0.7 1,623,828 - -1.5 1,623,828 - -2.0 1,623,828 - +0.1 1,623,828 - +0.7 1,623,847 + +1.7 1,623,847 + -1.2 1,623,848 - -0.9 1,623,848 - +2.3 1,623,848 - -0.1 1,623,848 - +0.5 1,623,848 - -0.1 1,623,851 + -1.9 1,623,851 + -0.0 1,623,851 + +0.6 1,623,852 - +1.1 1,623,852 - -0.8 1,623,852 - -0.6 1,623,852 - -0.5 1,623,852 - -2.7 1,623,884 + +0.4 1,623,885 - -0.7 1,623,885 - +0.6 1,623,968 + -0.5 1,623,968 + +0.2 1,623,969 - -1.6 1,623,969 - +0.1 1,624,047 + -2.0 1,624,047 + -0.2 1,624,211 - Psyr_1435 0.25 -0.9 1,624,211 - Psyr_1435 0.25 -0.2 1,624,273 - Psyr_1435 0.31 -0.6 1,624,318 + Psyr_1435 0.36 -0.1 1,624,318 + Psyr_1435 0.36 +1.1 1,624,318 + Psyr_1435 0.36 -0.8 1,624,339 + Psyr_1435 0.38 -1.6 1,624,339 + Psyr_1435 0.38 +1.0 1,624,339 + Psyr_1435 0.38 +2.9 1,624,339 + Psyr_1435 0.38 -2.7 1,624,339 + Psyr_1435 0.38 -1.2 1,624,339 + Psyr_1435 0.38 -1.3 1,624,339 + Psyr_1435 0.38 +1.7 1,624,339 + Psyr_1435 0.38 +2.1 1,624,339 + Psyr_1435 0.38 +1.1 1,624,340 - Psyr_1435 0.38 -2.1 1,624,340 - Psyr_1435 0.38 -0.1 1,624,340 - Psyr_1435 0.38 +0.0 1,624,340 - Psyr_1435 0.38 +0.6 1,624,340 - Psyr_1435 0.38 +0.1 1,624,340 - Psyr_1435 0.38 -1.2 1,624,385 + Psyr_1435 0.43 +2.3 1,624,386 - Psyr_1435 0.43 -3.3 1,624,402 + Psyr_1435 0.45 -1.5 1,624,402 + Psyr_1435 0.45 +0.7 1,624,403 - Psyr_1435 0.45 +1.1 1,624,455 + Psyr_1435 0.51 +0.5 1,624,455 + Psyr_1435 0.51 -0.7 1,624,455 + Psyr_1435 0.51 +0.0 1,624,456 - Psyr_1435 0.51 -3.0 1,624,456 - Psyr_1435 0.51 +4.3 1,624,456 - Psyr_1435 0.51 -0.2 1,624,456 - Psyr_1435 0.51 +1.1 1,624,456 - Psyr_1435 0.51 -0.3 1,624,459 + Psyr_1435 0.51 +0.2 1,624,459 + Psyr_1435 0.51 +0.5 1,624,459 + Psyr_1435 0.51 -0.7 1,624,460 - Psyr_1435 0.51 +0.4 1,624,460 - Psyr_1435 0.51 -1.5 1,624,553 + Psyr_1435 0.61 -1.0 1,624,553 + Psyr_1435 0.61 -1.7 1,624,553 + Psyr_1435 0.61 +0.2 1,624,553 + Psyr_1435 0.61 +1.7 1,624,553 + Psyr_1435 0.61 +2.5 1,624,553 + Psyr_1435 0.61 +0.9 1,624,553 + Psyr_1435 0.61 -0.1 1,624,554 - Psyr_1435 0.61 +0.0 1,624,554 - Psyr_1435 0.61 -0.5 1,624,554 - Psyr_1435 0.61 -0.3 1,624,554 - Psyr_1435 0.61 -1.5 1,624,723 + Psyr_1435 0.79 +1.8 1,624,723 + Psyr_1435 0.79 -0.5 1,624,724 - Psyr_1435 0.79 -0.9 1,624,724 - Psyr_1435 0.79 +0.5 1,624,724 - Psyr_1435 0.79 -1.2 1,624,724 - Psyr_1435 0.79 +1.1 1,624,754 + Psyr_1435 0.83 +0.7 1,624,754 + Psyr_1435 0.83 -0.6 1,624,754 + Psyr_1435 0.83 -0.3 1,624,761 - Psyr_1435 0.83 -1.3 1,624,807 - Psyr_1435 0.88 +0.6
Or see this region's nucleotide sequence