Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1333 and Psyr_1334 overlap by 10 nucleotides Psyr_1334 and Psyr_1335 overlap by 4 nucleotides Psyr_1335 and Psyr_1336 are separated by 23 nucleotides
Psyr_1333: Psyr_1333 - UBA/THIF-type NAD/FAD binding fold protein, at 1,512,659 to 1,513,489
_1333
Psyr_1334: Psyr_1334 - Cysteine desulfuration protein SufE, at 1,513,480 to 1,513,887
_1334
Psyr_1335: Psyr_1335 - Aminotransferase, class V, at 1,513,884 to 1,515,125
_1335
Psyr_1336: Psyr_1336 - tetrahydrodipicolinate succinylase, putative, at 1,515,149 to 1,516,183
_1336
Position (kb)
1513
1514
1515
1516 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1513.238 kb on + strand, within Psyr_1333 at 1513.238 kb on + strand, within Psyr_1333 at 1513.239 kb on - strand, within Psyr_1333 at 1513.297 kb on + strand, within Psyr_1333 at 1513.297 kb on + strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.333 kb on - strand, within Psyr_1333 at 1513.470 kb on + strand at 1513.646 kb on - strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.745 kb on - strand, within Psyr_1334 at 1513.951 kb on + strand at 1513.951 kb on + strand at 1513.952 kb on - strand at 1513.952 kb on - strand at 1514.225 kb on - strand, within Psyr_1335 at 1514.317 kb on + strand, within Psyr_1335 at 1514.417 kb on - strand, within Psyr_1335 at 1514.440 kb on + strand, within Psyr_1335 at 1514.441 kb on - strand, within Psyr_1335 at 1514.957 kb on - strand, within Psyr_1335 at 1514.959 kb on + strand, within Psyr_1335 at 1514.959 kb on + strand, within Psyr_1335 at 1514.959 kb on + strand, within Psyr_1335 at 1514.959 kb on + strand, within Psyr_1335 at 1514.960 kb on - strand, within Psyr_1335 at 1514.960 kb on - strand, within Psyr_1335 at 1514.960 kb on - strand, within Psyr_1335 at 1514.960 kb on - strand, within Psyr_1335 at 1514.960 kb on - strand, within Psyr_1335 at 1515.019 kb on + strand at 1515.019 kb on + strand at 1515.020 kb on - strand at 1515.020 kb on - strand at 1515.020 kb on - strand at 1515.155 kb on - strand at 1515.155 kb on - strand at 1515.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 1,513,238 + Psyr_1333 0.70 -0.5 1,513,238 + Psyr_1333 0.70 +0.7 1,513,239 - Psyr_1333 0.70 +0.2 1,513,297 + Psyr_1333 0.77 -0.1 1,513,297 + Psyr_1333 0.77 -1.0 1,513,298 - Psyr_1333 0.77 +0.9 1,513,298 - Psyr_1333 0.77 +0.1 1,513,298 - Psyr_1333 0.77 +0.1 1,513,298 - Psyr_1333 0.77 +0.5 1,513,333 - Psyr_1333 0.81 -0.2 1,513,470 + +0.6 1,513,646 - Psyr_1334 0.41 +1.7 1,513,744 + Psyr_1334 0.65 +0.9 1,513,744 + Psyr_1334 0.65 +3.9 1,513,744 + Psyr_1334 0.65 +0.8 1,513,744 + Psyr_1334 0.65 +0.2 1,513,744 + Psyr_1334 0.65 +0.7 1,513,745 - Psyr_1334 0.65 +2.3 1,513,951 + -0.4 1,513,951 + -0.5 1,513,952 - -0.4 1,513,952 - -1.5 1,514,225 - Psyr_1335 0.27 +1.4 1,514,317 + Psyr_1335 0.35 +1.7 1,514,417 - Psyr_1335 0.43 +0.6 1,514,440 + Psyr_1335 0.45 +0.6 1,514,441 - Psyr_1335 0.45 +0.1 1,514,957 - Psyr_1335 0.86 -0.4 1,514,959 + Psyr_1335 0.87 +0.1 1,514,959 + Psyr_1335 0.87 +0.5 1,514,959 + Psyr_1335 0.87 +0.6 1,514,959 + Psyr_1335 0.87 +0.9 1,514,960 - Psyr_1335 0.87 -1.1 1,514,960 - Psyr_1335 0.87 +0.8 1,514,960 - Psyr_1335 0.87 +0.5 1,514,960 - Psyr_1335 0.87 +0.4 1,514,960 - Psyr_1335 0.87 +0.5 1,515,019 + -0.4 1,515,019 + +0.8 1,515,020 - +1.0 1,515,020 - +0.7 1,515,020 - +0.1 1,515,155 - +0.4 1,515,155 - +0.0 1,515,155 - -0.3
Or see this region's nucleotide sequence