Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0425 and Psyr_0426 overlap by 4 nucleotides Psyr_0426 and Psyr_0427 overlap by 4 nucleotides Psyr_0427 and Psyr_0428 overlap by 4 nucleotides
Psyr_0425: Psyr_0425 - Beta-ketoacyl synthase:Beta-ketoacyl synthase, at 463,950 to 465,146
_0425
Psyr_0426: Psyr_0426 - lipoprotein, putative, at 465,143 to 465,625
_0426
Psyr_0427: Psyr_0427 - conserved hypothetical protein, at 465,622 to 466,356
_0427
Psyr_0428: Psyr_0428 - Monooxygenase, FAD-binding protein, at 466,353 to 467,600
_0428
Position (kb) 465
466 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 464.239 kb on - strand, within Psyr_0425 at 464.239 kb on - strand, within Psyr_0425 at 464.305 kb on + strand, within Psyr_0425 at 464.495 kb on + strand, within Psyr_0425 at 464.695 kb on + strand, within Psyr_0425 at 464.695 kb on + strand, within Psyr_0425 at 464.696 kb on - strand, within Psyr_0425 at 465.136 kb on + strand at 465.137 kb on - strand at 465.137 kb on - strand at 465.173 kb on - strand at 465.180 kb on + strand at 465.180 kb on + strand at 465.180 kb on + strand at 465.181 kb on - strand at 465.181 kb on - strand at 465.181 kb on - strand at 465.181 kb on - strand at 465.181 kb on - strand at 465.228 kb on + strand, within Psyr_0426 at 465.614 kb on + strand at 465.758 kb on + strand, within Psyr_0427 at 465.758 kb on + strand, within Psyr_0427 at 465.759 kb on - strand, within Psyr_0427 at 465.759 kb on - strand, within Psyr_0427 at 465.818 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.887 kb on + strand, within Psyr_0427 at 465.888 kb on - strand, within Psyr_0427 at 465.918 kb on - strand, within Psyr_0427 at 465.918 kb on - strand, within Psyr_0427 at 466.066 kb on + strand, within Psyr_0427 at 466.287 kb on + strand at 466.328 kb on + strand at 466.328 kb on + strand at 466.328 kb on + strand at 466.328 kb on + strand at 466.328 kb on + strand at 466.328 kb on + strand at 466.328 kb on + strand at 466.328 kb on + strand at 466.329 kb on - strand at 466.329 kb on - strand at 466.329 kb on - strand at 466.329 kb on - strand at 466.329 kb on - strand at 466.329 kb on - strand at 466.329 kb on - strand at 466.329 kb on - strand at 466.442 kb on - strand at 466.517 kb on + strand, within Psyr_0428 at 466.517 kb on + strand, within Psyr_0428 at 466.518 kb on - strand, within Psyr_0428 at 466.540 kb on + strand, within Psyr_0428 at 466.541 kb on - strand, within Psyr_0428
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 464,239 - Psyr_0425 0.24 +1.3 464,239 - Psyr_0425 0.24 +0.2 464,305 + Psyr_0425 0.30 +0.4 464,495 + Psyr_0425 0.46 +0.6 464,695 + Psyr_0425 0.62 +1.7 464,695 + Psyr_0425 0.62 +0.8 464,696 - Psyr_0425 0.62 -0.4 465,136 + +1.3 465,137 - -1.5 465,137 - -0.2 465,173 - +1.6 465,180 + +0.7 465,180 + -2.9 465,180 + -0.4 465,181 - -1.0 465,181 - +0.3 465,181 - -0.7 465,181 - +0.0 465,181 - +0.3 465,228 + Psyr_0426 0.18 +1.1 465,614 + -0.3 465,758 + Psyr_0427 0.19 +0.1 465,758 + Psyr_0427 0.19 +1.5 465,759 - Psyr_0427 0.19 -0.1 465,759 - Psyr_0427 0.19 +0.2 465,818 + Psyr_0427 0.27 -0.5 465,887 + Psyr_0427 0.36 +0.7 465,887 + Psyr_0427 0.36 +1.0 465,887 + Psyr_0427 0.36 -1.7 465,887 + Psyr_0427 0.36 -0.0 465,887 + Psyr_0427 0.36 +1.0 465,887 + Psyr_0427 0.36 +0.8 465,887 + Psyr_0427 0.36 +1.3 465,887 + Psyr_0427 0.36 -0.1 465,887 + Psyr_0427 0.36 +0.5 465,887 + Psyr_0427 0.36 -0.6 465,887 + Psyr_0427 0.36 -1.2 465,888 - Psyr_0427 0.36 +0.8 465,918 - Psyr_0427 0.40 +1.3 465,918 - Psyr_0427 0.40 -0.1 466,066 + Psyr_0427 0.60 -0.4 466,287 + -1.5 466,328 + -0.1 466,328 + -2.1 466,328 + -0.0 466,328 + +0.9 466,328 + -1.6 466,328 + +0.6 466,328 + +0.5 466,328 + +2.7 466,329 - +2.3 466,329 - -1.1 466,329 - -0.8 466,329 - +3.0 466,329 - +0.1 466,329 - -0.0 466,329 - -1.4 466,329 - -0.5 466,442 - -2.0 466,517 + Psyr_0428 0.13 +0.8 466,517 + Psyr_0428 0.13 -0.1 466,518 - Psyr_0428 0.13 -1.2 466,540 + Psyr_0428 0.15 -0.7 466,541 - Psyr_0428 0.15 +0.2
Or see this region's nucleotide sequence