Experiment: KB with Lupeol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0012 and Psyr_0013 are separated by 20 nucleotides Psyr_0013 and Psyr_0014 are separated by 38 nucleotides
Psyr_0012: Psyr_0012 - glycyl-tRNA synthetase alpha chain, at 13,967 to 14,974
_0012
Psyr_0013: Psyr_0013 - DNA-3-methyladenine glycosylase I, at 14,995 to 15,546
_0013
Psyr_0014: Psyr_0014 - lipid A biosynthesis acyltransferase, at 15,585 to 16,472
_0014
Position (kb)
14
15
16 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 14.956 kb on - strand at 14.956 kb on - strand at 14.994 kb on + strand at 15.031 kb on + strand at 15.031 kb on + strand at 15.031 kb on + strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.041 kb on - strand at 15.041 kb on - strand at 15.170 kb on + strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.476 kb on - strand, within Psyr_0013 at 15.476 kb on - strand, within Psyr_0013 at 15.591 kb on - strand at 15.975 kb on + strand, within Psyr_0014 at 15.975 kb on + strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.335 kb on + strand, within Psyr_0014 at 16.335 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.389 kb on + strand at 16.389 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.414 kb on - strand at 16.414 kb on - strand at 16.467 kb on + strand at 16.467 kb on + strand at 16.470 kb on + strand at 16.471 kb on - strand at 16.471 kb on - strand at 16.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Lupeol 10 mM remove 14,956 - -1.4 14,956 - +0.0 14,994 + -0.3 15,031 + +0.4 15,031 + +1.0 15,031 + +0.3 15,032 - +1.3 15,032 - -0.4 15,032 - -0.7 15,032 - +0.5 15,032 - +1.4 15,032 - +1.1 15,032 - +2.4 15,032 - -2.7 15,032 - +0.0 15,040 + -0.2 15,040 + -1.0 15,040 + +2.1 15,040 + -1.3 15,041 - -0.4 15,041 - +0.2 15,170 + Psyr_0013 0.32 -0.7 15,171 - Psyr_0013 0.32 -0.7 15,171 - Psyr_0013 0.32 +1.4 15,171 - Psyr_0013 0.32 +0.5 15,475 + Psyr_0013 0.87 +1.4 15,475 + Psyr_0013 0.87 +0.7 15,475 + Psyr_0013 0.87 +0.5 15,475 + Psyr_0013 0.87 +2.6 15,475 + Psyr_0013 0.87 +0.9 15,475 + Psyr_0013 0.87 -0.3 15,475 + Psyr_0013 0.87 -0.1 15,476 - Psyr_0013 0.87 +0.2 15,476 - Psyr_0013 0.87 -2.7 15,591 - -0.2 15,975 + Psyr_0014 0.44 -0.6 15,975 + Psyr_0014 0.44 +1.4 16,302 - Psyr_0014 0.81 +1.4 16,302 - Psyr_0014 0.81 -0.2 16,302 - Psyr_0014 0.81 +1.4 16,335 + Psyr_0014 0.84 +1.4 16,335 - Psyr_0014 0.84 +1.4 16,336 - Psyr_0014 0.85 -0.9 16,336 - Psyr_0014 0.85 +1.4 16,336 - Psyr_0014 0.85 -0.6 16,336 - Psyr_0014 0.85 +0.4 16,336 - Psyr_0014 0.85 +0.4 16,336 - Psyr_0014 0.85 -0.2 16,389 + +2.4 16,389 + -0.9 16,413 + -0.2 16,413 + +1.4 16,413 + -1.2 16,413 + -0.6 16,414 - +1.4 16,414 - +1.4 16,467 + -1.4 16,467 + +0.2 16,470 + -2.0 16,471 - +1.9 16,471 - -0.3 16,471 - -0.1
Or see this region's nucleotide sequence