Experiment: BHIS with Dimethyl Sulfoxide 0.05 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1420 and BT1421 are separated by 86 nucleotides BT1421 and BT1422 are separated by 196 nucleotides BT1422 and BT1423 are separated by 2 nucleotides BT1423 and BT1424 are separated by 25 nucleotides
BT1420: BT1420 - conserved hypothetical protein (NCBI ptt file), at 1,753,097 to 1,755,253
BT1420
BT1421: BT1421 - hypothetical protein (NCBI ptt file), at 1,755,340 to 1,755,654
BT1421
BT1422: BT1422 - conserved hypothetical protein (NCBI ptt file), at 1,755,851 to 1,756,195
BT1422
BT1423: BT1423 - conserved hypothetical protein (NCBI ptt file), at 1,756,198 to 1,756,584
BT1423
BT1424: BT1424 - hypothetical protein (NCBI ptt file), at 1,756,610 to 1,757,227
BT1424
Position (kb)
1755
1756
1757 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1754.948 kb on + strand, within BT1420 at 1755.013 kb on + strand, within BT1420 at 1755.013 kb on + strand, within BT1420 at 1755.169 kb on + strand at 1755.170 kb on - strand at 1755.170 kb on - strand at 1755.212 kb on + strand at 1755.238 kb on + strand at 1755.272 kb on + strand at 1755.301 kb on + strand at 1755.302 kb on - strand at 1755.337 kb on + strand at 1755.341 kb on + strand at 1755.408 kb on + strand, within BT1421 at 1755.408 kb on + strand, within BT1421 at 1755.485 kb on + strand, within BT1421 at 1755.610 kb on + strand, within BT1421 at 1755.623 kb on - strand at 1755.626 kb on + strand at 1755.626 kb on + strand at 1755.650 kb on + strand at 1755.851 kb on - strand at 1755.870 kb on + strand at 1755.871 kb on - strand at 1755.933 kb on + strand, within BT1422 at 1755.966 kb on + strand, within BT1422 at 1756.021 kb on - strand, within BT1422 at 1756.045 kb on - strand, within BT1422 at 1756.125 kb on + strand, within BT1422 at 1756.126 kb on - strand, within BT1422 at 1756.138 kb on + strand, within BT1422 at 1756.138 kb on + strand, within BT1422 at 1756.182 kb on + strand at 1756.182 kb on + strand at 1756.185 kb on + strand at 1756.190 kb on - strand at 1756.220 kb on + strand at 1756.221 kb on - strand at 1756.221 kb on - strand at 1756.221 kb on - strand at 1756.347 kb on + strand, within BT1423 at 1756.347 kb on + strand, within BT1423 at 1756.352 kb on + strand, within BT1423 at 1756.353 kb on - strand, within BT1423 at 1756.421 kb on + strand, within BT1423 at 1756.422 kb on - strand, within BT1423 at 1756.422 kb on - strand, within BT1423 at 1756.479 kb on - strand, within BT1423 at 1756.479 kb on - strand, within BT1423 at 1756.515 kb on - strand, within BT1423 at 1756.519 kb on - strand, within BT1423 at 1756.569 kb on - strand at 1756.579 kb on - strand at 1756.592 kb on - strand at 1756.632 kb on + strand at 1756.742 kb on + strand, within BT1424 at 1756.948 kb on + strand, within BT1424 at 1756.960 kb on + strand, within BT1424 at 1757.016 kb on + strand, within BT1424 at 1757.016 kb on + strand, within BT1424 at 1757.017 kb on - strand, within BT1424 at 1757.063 kb on + strand, within BT1424 at 1757.075 kb on + strand, within BT1424 at 1757.105 kb on + strand, within BT1424 at 1757.117 kb on - strand, within BT1424 at 1757.133 kb on + strand, within BT1424 at 1757.182 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.05 vol% remove 1,754,948 + BT1420 0.86 +0.7 1,755,013 + BT1420 0.89 -3.3 1,755,013 + BT1420 0.89 -1.6 1,755,169 + +1.1 1,755,170 - -1.5 1,755,170 - -2.5 1,755,212 + -0.9 1,755,238 + -0.9 1,755,272 + -1.9 1,755,301 + +0.5 1,755,302 - -1.9 1,755,337 + +0.1 1,755,341 + +0.1 1,755,408 + BT1421 0.22 -0.9 1,755,408 + BT1421 0.22 -1.5 1,755,485 + BT1421 0.46 -2.3 1,755,610 + BT1421 0.86 -1.5 1,755,623 - -1.6 1,755,626 + -0.9 1,755,626 + +0.1 1,755,650 + +0.1 1,755,851 - -1.0 1,755,870 + +0.1 1,755,871 - -0.9 1,755,933 + BT1422 0.24 -2.2 1,755,966 + BT1422 0.33 -2.2 1,756,021 - BT1422 0.49 -2.9 1,756,045 - BT1422 0.56 -1.2 1,756,125 + BT1422 0.79 -1.9 1,756,126 - BT1422 0.80 -1.9 1,756,138 + BT1422 0.83 -0.3 1,756,138 + BT1422 0.83 -2.5 1,756,182 + -1.3 1,756,182 + +0.1 1,756,185 + +0.1 1,756,190 - -1.9 1,756,220 + -2.2 1,756,221 - -3.5 1,756,221 - -2.5 1,756,221 - -1.9 1,756,347 + BT1423 0.39 +2.5 1,756,347 + BT1423 0.39 -0.9 1,756,352 + BT1423 0.40 -1.0 1,756,353 - BT1423 0.40 +0.1 1,756,421 + BT1423 0.58 -0.3 1,756,422 - BT1423 0.58 -1.9 1,756,422 - BT1423 0.58 -1.5 1,756,479 - BT1423 0.73 +0.1 1,756,479 - BT1423 0.73 -1.5 1,756,515 - BT1423 0.82 +1.7 1,756,519 - BT1423 0.83 -2.7 1,756,569 - +0.1 1,756,579 - +2.1 1,756,592 - -1.5 1,756,632 + -1.0 1,756,742 + BT1424 0.21 -0.5 1,756,948 + BT1424 0.55 -1.7 1,756,960 + BT1424 0.57 +0.1 1,757,016 + BT1424 0.66 -0.6 1,757,016 + BT1424 0.66 -1.7 1,757,017 - BT1424 0.66 -1.5 1,757,063 + BT1424 0.73 -1.2 1,757,075 + BT1424 0.75 +0.1 1,757,105 + BT1424 0.80 -1.5 1,757,117 - BT1424 0.82 -1.3 1,757,133 + BT1424 0.85 -3.2 1,757,182 + +0.1
Or see this region's nucleotide sequence