Experiment: MoLS4 with L-Alanine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysS and tRNA-Asn are separated by 190 nucleotides tRNA-Asn and DVU2377 are separated by 347 nucleotides DVU2377 and foxR are separated by 109 nucleotides foxR and pqqL are separated by 124 nucleotides
DVU2376: lysS - lysyl-tRNA synthetase (TIGR), at 2,473,176 to 2,474,759
lysS
DVU_tRNA-Asn-1: tRNA-Asn - tRNA with anticodon GTT for Asn (VIMSS-AUTO), at 2,474,950 to 2,475,025
tRNA-Asn
DVU2377: DVU2377 - hypothetical protein (TIGR), at 2,475,373 to 2,475,504
DVU2377
DVU2378: foxR - transcriptional regulator, AraC family (TIGR), at 2,475,614 to 2,476,306
foxR
DVU2379: pqqL - peptidase, M16 family, putative (TIGR), at 2,476,431 to 2,479,448
pqqL
Position (kb)
2475
2476 Strain fitness (log2 ratio)
-1
0
1 at 2474.924 kb on + strand at 2475.136 kb on - strand at 2475.158 kb on - strand at 2475.165 kb on + strand at 2475.173 kb on - strand at 2475.235 kb on - strand at 2475.254 kb on - strand at 2475.255 kb on + strand at 2475.255 kb on + strand at 2475.256 kb on - strand at 2475.258 kb on + strand at 2475.261 kb on - strand at 2475.266 kb on - strand at 2475.472 kb on + strand, within DVU2377 at 2475.490 kb on - strand, within DVU2377 at 2475.557 kb on - strand at 2475.614 kb on + strand at 2475.624 kb on + strand at 2475.658 kb on - strand at 2475.668 kb on + strand at 2475.671 kb on - strand at 2475.696 kb on + strand, within foxR at 2475.704 kb on - strand, within foxR at 2475.712 kb on + strand, within foxR at 2475.726 kb on + strand, within foxR at 2475.780 kb on - strand, within foxR at 2475.802 kb on + strand, within foxR at 2475.868 kb on - strand, within foxR at 2475.904 kb on - strand, within foxR at 2475.914 kb on + strand, within foxR at 2475.917 kb on - strand, within foxR at 2475.939 kb on - strand, within foxR at 2476.136 kb on + strand, within foxR at 2476.145 kb on + strand, within foxR at 2476.189 kb on + strand, within foxR at 2476.262 kb on + strand at 2476.433 kb on - strand at 2476.433 kb on - strand at 2476.454 kb on + strand at 2476.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Alanine as nutrient 10mM remove 2,474,924 + -1.1 2,475,136 - +0.1 2,475,158 - -0.4 2,475,165 + -0.8 2,475,173 - -1.5 2,475,235 - -0.1 2,475,254 - +0.0 2,475,255 + -0.7 2,475,255 + -0.1 2,475,256 - -0.3 2,475,258 + +0.0 2,475,261 - -1.0 2,475,266 - +0.4 2,475,472 + DVU2377 0.75 -1.1 2,475,490 - DVU2377 0.89 -1.2 2,475,557 - -0.2 2,475,614 + +0.2 2,475,624 + -0.1 2,475,658 - -0.0 2,475,668 + -0.2 2,475,671 - -1.3 2,475,696 + foxR DVU2378 0.12 -0.7 2,475,704 - foxR DVU2378 0.13 +0.9 2,475,712 + foxR DVU2378 0.14 -0.4 2,475,726 + foxR DVU2378 0.16 +0.4 2,475,780 - foxR DVU2378 0.24 +0.0 2,475,802 + foxR DVU2378 0.27 +0.9 2,475,868 - foxR DVU2378 0.37 +0.3 2,475,904 - foxR DVU2378 0.42 +0.5 2,475,914 + foxR DVU2378 0.43 -1.0 2,475,917 - foxR DVU2378 0.44 -0.1 2,475,939 - foxR DVU2378 0.47 -0.5 2,476,136 + foxR DVU2378 0.75 +0.2 2,476,145 + foxR DVU2378 0.77 -1.3 2,476,189 + foxR DVU2378 0.83 +0.1 2,476,262 + -1.5 2,476,433 - +0.8 2,476,433 - +0.1 2,476,454 + +1.0 2,476,490 - -0.5
Or see this region's nucleotide sequence