Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0004

Experiment: KB with Nonanoic 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0003 and Psyr_0004 are separated by 4 nucleotidesPsyr_0004 and Psyr_0005 are separated by 55 nucleotidesPsyr_0005 and Psyr_0006 are separated by 27 nucleotides Psyr_0003: Psyr_0003 - DNA replication and repair protein RecF, at 2,701 to 3,804 _0003 Psyr_0004: Psyr_0004 - DNA gyrase subunit B, at 3,809 to 6,226 _0004 Psyr_0005: Psyr_0005 - conserved hypothetical protein, at 6,282 to 7,169 _0005 Psyr_0006: Psyr_0006 - putative phenylacetaldoxime dehydratase, at 7,197 to 8,231 _0006 Position (kb) 3 4 5 6 7Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2.827 kb on - strand, within Psyr_0003at 2.887 kb on + strand, within Psyr_0003at 3.095 kb on + strand, within Psyr_0003at 3.095 kb on + strand, within Psyr_0003at 3.207 kb on + strand, within Psyr_0003at 3.207 kb on + strand, within Psyr_0003at 3.289 kb on + strand, within Psyr_0003at 3.289 kb on + strand, within Psyr_0003at 3.289 kb on + strand, within Psyr_0003at 3.289 kb on + strand, within Psyr_0003at 3.289 kb on + strand, within Psyr_0003at 3.291 kb on + strand, within Psyr_0003at 3.291 kb on + strand, within Psyr_0003at 3.291 kb on + strand, within Psyr_0003at 3.291 kb on + strand, within Psyr_0003at 3.291 kb on + strand, within Psyr_0003at 3.291 kb on + strand, within Psyr_0003at 3.364 kb on + strand, within Psyr_0003at 3.364 kb on + strand, within Psyr_0003at 3.364 kb on + strand, within Psyr_0003at 3.364 kb on + strand, within Psyr_0003at 3.367 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.395 kb on + strand, within Psyr_0003at 3.399 kb on + strand, within Psyr_0003at 3.399 kb on + strand, within Psyr_0003at 3.399 kb on + strand, within Psyr_0003at 3.399 kb on + strand, within Psyr_0003at 3.399 kb on + strand, within Psyr_0003at 3.480 kb on + strand, within Psyr_0003at 3.480 kb on + strand, within Psyr_0003at 3.507 kb on + strand, within Psyr_0003at 3.507 kb on + strand, within Psyr_0003at 3.552 kb on + strand, within Psyr_0003at 3.597 kb on + strand, within Psyr_0003at 3.597 kb on + strand, within Psyr_0003at 3.597 kb on + strand, within Psyr_0003at 3.597 kb on + strand, within Psyr_0003at 3.601 kb on + strand, within Psyr_0003at 3.601 kb on + strand, within Psyr_0003at 3.601 kb on + strand, within Psyr_0003at 3.601 kb on + strand, within Psyr_0003at 3.601 kb on + strand, within Psyr_0003at 3.608 kb on + strand, within Psyr_0003at 3.608 kb on + strand, within Psyr_0003at 3.608 kb on + strand, within Psyr_0003at 3.674 kb on + strand, within Psyr_0003at 3.690 kb on + strand, within Psyr_0003at 3.701 kb on + strandat 3.701 kb on + strandat 6.233 kb on + strandat 6.233 kb on + strandat 6.233 kb on + strandat 6.234 kb on - strandat 6.234 kb on - strandat 6.284 kb on - strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.345 kb on + strandat 6.346 kb on - strandat 6.346 kb on - strandat 6.346 kb on - strandat 6.346 kb on - strandat 6.346 kb on - strandat 6.346 kb on - strandat 6.346 kb on - strandat 6.346 kb on - strandat 6.346 kb on - strandat 6.346 kb on - strandat 6.346 kb on - strandat 6.415 kb on + strand, within Psyr_0005at 6.415 kb on + strand, within Psyr_0005at 6.415 kb on + strand, within Psyr_0005at 6.415 kb on + strand, within Psyr_0005at 6.416 kb on - strand, within Psyr_0005at 6.416 kb on - strand, within Psyr_0005at 6.416 kb on - strand, within Psyr_0005at 6.416 kb on - strand, within Psyr_0005at 6.419 kb on + strand, within Psyr_0005at 6.419 kb on + strand, within Psyr_0005at 6.420 kb on - strand, within Psyr_0005at 6.420 kb on - strand, within Psyr_0005at 6.420 kb on - strand, within Psyr_0005at 6.420 kb on - strand, within Psyr_0005at 6.420 kb on - strand, within Psyr_0005at 6.420 kb on - strand, within Psyr_0005at 6.476 kb on + strand, within Psyr_0005at 6.477 kb on - strand, within Psyr_0005at 6.520 kb on + strand, within Psyr_0005at 6.520 kb on + strand, within Psyr_0005at 6.521 kb on - strand, within Psyr_0005at 6.521 kb on - strand, within Psyr_0005at 6.521 kb on - strand, within Psyr_0005at 6.521 kb on - strand, within Psyr_0005at 6.529 kb on + strand, within Psyr_0005at 6.530 kb on - strand, within Psyr_0005at 6.530 kb on - strand, within Psyr_0005at 6.583 kb on + strand, within Psyr_0005at 6.584 kb on - strand, within Psyr_0005at 6.658 kb on + strand, within Psyr_0005at 6.658 kb on + strand, within Psyr_0005at 6.658 kb on + strand, within Psyr_0005at 6.658 kb on + strand, within Psyr_0005at 6.658 kb on + strand, within Psyr_0005at 6.659 kb on - strand, within Psyr_0005at 6.659 kb on - strand, within Psyr_0005at 6.659 kb on - strand, within Psyr_0005at 6.659 kb on - strand, within Psyr_0005at 6.659 kb on - strand, within Psyr_0005at 6.659 kb on - strand, within Psyr_0005at 6.659 kb on - strand, within Psyr_0005at 6.659 kb on - strand, within Psyr_0005at 6.659 kb on - strand, within Psyr_0005at 6.721 kb on + strand, within Psyr_0005at 6.721 kb on + strand, within Psyr_0005at 6.740 kb on - strand, within Psyr_0005at 6.740 kb on - strand, within Psyr_0005at 6.740 kb on - strand, within Psyr_0005at 6.770 kb on + strand, within Psyr_0005at 6.770 kb on + strand, within Psyr_0005at 6.770 kb on + strand, within Psyr_0005at 6.770 kb on + strand, within Psyr_0005at 6.770 kb on + strand, within Psyr_0005at 6.770 kb on + strand, within Psyr_0005at 6.770 kb on + strand, within Psyr_0005at 6.771 kb on - strand, within Psyr_0005at 6.771 kb on - strand, within Psyr_0005at 6.771 kb on - strand, within Psyr_0005at 6.771 kb on - strand, within Psyr_0005at 6.783 kb on + strand, within Psyr_0005at 6.900 kb on + strand, within Psyr_0005at 6.901 kb on - strand, within Psyr_0005at 6.901 kb on - strand, within Psyr_0005at 6.937 kb on + strand, within Psyr_0005at 6.937 kb on + strand, within Psyr_0005at 6.937 kb on + strand, within Psyr_0005at 6.937 kb on + strand, within Psyr_0005at 6.938 kb on - strand, within Psyr_0005at 6.938 kb on - strand, within Psyr_0005at 6.938 kb on - strand, within Psyr_0005at 6.938 kb on - strand, within Psyr_0005at 7.001 kb on - strand, within Psyr_0005at 7.030 kb on + strand, within Psyr_0005at 7.031 kb on - strand, within Psyr_0005at 7.070 kb on - strand, within Psyr_0005at 7.125 kb on - strandat 7.169 kb on + strandat 7.169 kb on + strandat 7.170 kb on - strandat 7.170 kb on - strandat 7.170 kb on - strandat 7.170 kb on - strandat 7.188 kb on + strandat 7.189 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Nonanoic 10 mM
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2,827 - Psyr_0003 0.11 -0.6
2,887 + Psyr_0003 0.17 -2.3
3,095 + Psyr_0003 0.36 +0.2
3,095 + Psyr_0003 0.36 -0.7
3,207 + Psyr_0003 0.46 -3.4
3,207 + Psyr_0003 0.46 -0.1
3,289 + Psyr_0003 0.53 -0.3
3,289 + Psyr_0003 0.53 +0.0
3,289 + Psyr_0003 0.53 -0.7
3,289 + Psyr_0003 0.53 -1.3
3,289 + Psyr_0003 0.53 +0.1
3,291 + Psyr_0003 0.53 -0.2
3,291 + Psyr_0003 0.53 -0.9
3,291 + Psyr_0003 0.53 -0.3
3,291 + Psyr_0003 0.53 +0.9
3,291 + Psyr_0003 0.53 +0.1
3,291 + Psyr_0003 0.53 -1.1
3,364 + Psyr_0003 0.60 +1.3
3,364 + Psyr_0003 0.60 -0.7
3,364 + Psyr_0003 0.60 +0.3
3,364 + Psyr_0003 0.60 -0.7
3,367 + Psyr_0003 0.60 -1.9
3,395 + Psyr_0003 0.63 -0.5
3,395 + Psyr_0003 0.63 -0.4
3,395 + Psyr_0003 0.63 -0.7
3,395 + Psyr_0003 0.63 -1.4
3,395 + Psyr_0003 0.63 -0.4
3,395 + Psyr_0003 0.63 -0.8
3,395 + Psyr_0003 0.63 -0.7
3,395 + Psyr_0003 0.63 -0.0
3,395 + Psyr_0003 0.63 +4.1
3,395 + Psyr_0003 0.63 -0.8
3,395 + Psyr_0003 0.63 -0.3
3,395 + Psyr_0003 0.63 +1.3
3,395 + Psyr_0003 0.63 +0.9
3,395 + Psyr_0003 0.63 -0.9
3,395 + Psyr_0003 0.63 -2.1
3,395 + Psyr_0003 0.63 +0.4
3,395 + Psyr_0003 0.63 -0.6
3,399 + Psyr_0003 0.63 -0.3
3,399 + Psyr_0003 0.63 +0.7
3,399 + Psyr_0003 0.63 -0.1
3,399 + Psyr_0003 0.63 +0.3
3,399 + Psyr_0003 0.63 -0.8
3,480 + Psyr_0003 0.71 -0.8
3,480 + Psyr_0003 0.71 -0.7
3,507 + Psyr_0003 0.73 +0.0
3,507 + Psyr_0003 0.73 -0.5
3,552 + Psyr_0003 0.77 -0.6
3,597 + Psyr_0003 0.81 -1.0
3,597 + Psyr_0003 0.81 -0.2
3,597 + Psyr_0003 0.81 -0.1
3,597 + Psyr_0003 0.81 -2.0
3,601 + Psyr_0003 0.82 +0.6
3,601 + Psyr_0003 0.82 +0.6
3,601 + Psyr_0003 0.82 -1.0
3,601 + Psyr_0003 0.82 +0.8
3,601 + Psyr_0003 0.82 +0.0
3,608 + Psyr_0003 0.82 -0.1
3,608 + Psyr_0003 0.82 -0.6
3,608 + Psyr_0003 0.82 -1.3
3,674 + Psyr_0003 0.88 -2.7
3,690 + Psyr_0003 0.90 +0.2
3,701 + -0.6
3,701 + +0.4
6,233 + -0.1
6,233 + -0.2
6,233 + +1.3
6,234 - -1.4
6,234 - +3.0
6,284 - +1.2
6,345 + -0.7
6,345 + -0.4
6,345 + +1.0
6,345 + -0.6
6,345 + -1.4
6,345 + +0.9
6,345 + +0.5
6,345 + -1.0
6,345 + +0.2
6,345 + +1.0
6,345 + -2.0
6,345 + +0.7
6,346 - -0.5
6,346 - -0.7
6,346 - -0.7
6,346 - +1.8
6,346 - -0.4
6,346 - -1.3
6,346 - +0.1
6,346 - +0.4
6,346 - +0.9
6,346 - +1.0
6,346 - -1.1
6,415 + Psyr_0005 0.15 +1.0
6,415 + Psyr_0005 0.15 +0.6
6,415 + Psyr_0005 0.15 -0.1
6,415 + Psyr_0005 0.15 +0.5
6,416 - Psyr_0005 0.15 -0.1
6,416 - Psyr_0005 0.15 +0.4
6,416 - Psyr_0005 0.15 +0.4
6,416 - Psyr_0005 0.15 -0.7
6,419 + Psyr_0005 0.15 -0.3
6,419 + Psyr_0005 0.15 -1.7
6,420 - Psyr_0005 0.16 -0.7
6,420 - Psyr_0005 0.16 +0.7
6,420 - Psyr_0005 0.16 +0.4
6,420 - Psyr_0005 0.16 -1.6
6,420 - Psyr_0005 0.16 +0.6
6,420 - Psyr_0005 0.16 +1.1
6,476 + Psyr_0005 0.22 +1.4
6,477 - Psyr_0005 0.22 -1.7
6,520 + Psyr_0005 0.27 +1.8
6,520 + Psyr_0005 0.27 +0.7
6,521 - Psyr_0005 0.27 +0.2
6,521 - Psyr_0005 0.27 -0.9
6,521 - Psyr_0005 0.27 -0.9
6,521 - Psyr_0005 0.27 -0.1
6,529 + Psyr_0005 0.28 -0.7
6,530 - Psyr_0005 0.28 -0.3
6,530 - Psyr_0005 0.28 +0.3
6,583 + Psyr_0005 0.34 -0.7
6,584 - Psyr_0005 0.34 -2.2
6,658 + Psyr_0005 0.42 +0.8
6,658 + Psyr_0005 0.42 +0.1
6,658 + Psyr_0005 0.42 -0.3
6,658 + Psyr_0005 0.42 +0.3
6,658 + Psyr_0005 0.42 -1.7
6,659 - Psyr_0005 0.42 -0.6
6,659 - Psyr_0005 0.42 -1.8
6,659 - Psyr_0005 0.42 +0.4
6,659 - Psyr_0005 0.42 -0.9
6,659 - Psyr_0005 0.42 -0.6
6,659 - Psyr_0005 0.42 -0.1
6,659 - Psyr_0005 0.42 +0.2
6,659 - Psyr_0005 0.42 -1.9
6,659 - Psyr_0005 0.42 -0.3
6,721 + Psyr_0005 0.49 +0.8
6,721 + Psyr_0005 0.49 +0.3
6,740 - Psyr_0005 0.52 -1.4
6,740 - Psyr_0005 0.52 -0.7
6,740 - Psyr_0005 0.52 +0.3
6,770 + Psyr_0005 0.55 -0.6
6,770 + Psyr_0005 0.55 +0.9
6,770 + Psyr_0005 0.55 +0.1
6,770 + Psyr_0005 0.55 -0.9
6,770 + Psyr_0005 0.55 -0.9
6,770 + Psyr_0005 0.55 -0.3
6,770 + Psyr_0005 0.55 -1.7
6,771 - Psyr_0005 0.55 +0.3
6,771 - Psyr_0005 0.55 -0.7
6,771 - Psyr_0005 0.55 -0.7
6,771 - Psyr_0005 0.55 -1.4
6,783 + Psyr_0005 0.56 -0.4
6,900 + Psyr_0005 0.70 +0.2
6,901 - Psyr_0005 0.70 +0.7
6,901 - Psyr_0005 0.70 -1.2
6,937 + Psyr_0005 0.74 -0.6
6,937 + Psyr_0005 0.74 -1.3
6,937 + Psyr_0005 0.74 +0.8
6,937 + Psyr_0005 0.74 +2.8
6,938 - Psyr_0005 0.74 +0.1
6,938 - Psyr_0005 0.74 +0.3
6,938 - Psyr_0005 0.74 +0.9
6,938 - Psyr_0005 0.74 -0.8
7,001 - Psyr_0005 0.81 +0.8
7,030 + Psyr_0005 0.84 -0.0
7,031 - Psyr_0005 0.84 -0.9
7,070 - Psyr_0005 0.89 +0.5
7,125 - +0.1
7,169 + +0.3
7,169 + -1.2
7,170 - -0.7
7,170 - -1.3
7,170 - +1.3
7,170 - -1.2
7,188 + +1.1
7,189 - -1.2

Or see this region's nucleotide sequence