Strain Fitness in Methanococcus maripaludis S2 around MMP_RS00785

Experiment: H2 McCas with 1 mM (NH4)2S control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS00775 and MMP_RS00780 are separated by 17 nucleotidesMMP_RS00780 and MMP_RS00785 are separated by 56 nucleotidesMMP_RS00785 and MMP_RS00795 are separated by 626 nucleotides MMP_RS00775: MMP_RS00775 - threonine synthase, at 141,612 to 142,826 _RS00775 MMP_RS00780: MMP_RS00780 - 3-isopropylmalate dehydratase small subunit, at 142,844 to 143,347 _RS00780 MMP_RS00785: MMP_RS00785 - deoxyhypusine synthase, at 143,404 to 144,411 _RS00785 MMP_RS00795: MMP_RS00795 - formate dehydrogenase H subunit alpha selenocysteine-containing, at 145,038 to 147,068 _RS00795 Position (kb) 143 144 145Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 142.920 kb on + strandat 142.928 kb on - strandat 142.928 kb on - strandat 142.930 kb on - strandat 142.931 kb on + strandat 143.080 kb on + strandat 143.430 kb on - strandat 143.606 kb on - strandat 143.624 kb on + strandat 143.668 kb on - strandat 143.825 kb on + strandat 143.839 kb on + strandat 143.842 kb on - strandat 143.842 kb on - strandat 143.843 kb on + strandat 143.843 kb on + strandat 143.843 kb on + strandat 143.846 kb on - strandat 143.847 kb on + strandat 143.860 kb on - strandat 143.860 kb on - strandat 143.860 kb on - strandat 143.861 kb on + strandat 143.861 kb on + strandat 143.868 kb on - strandat 143.915 kb on - strandat 144.007 kb on - strandat 144.007 kb on - strandat 144.016 kb on + strandat 144.016 kb on + strandat 144.064 kb on + strandat 144.131 kb on - strandat 144.253 kb on - strandat 144.253 kb on - strandat 144.254 kb on + strandat 144.406 kb on + strandat 144.511 kb on + strandat 144.537 kb on - strandat 144.537 kb on - strandat 144.709 kb on - strandat 144.899 kb on - strandat 144.960 kb on + strandat 145.061 kb on - strandat 145.066 kb on - strandat 145.066 kb on - strandat 145.105 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas with 1 mM (NH4)2S control
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142,920 + -1.8
142,928 - -2.2
142,928 - -1.3
142,930 - -3.3
142,931 + -3.6
143,080 + -3.1
143,430 - -2.6
143,606 - -2.7
143,624 + -1.5
143,668 - -3.7
143,825 + -3.7
143,839 + -3.5
143,842 - -2.7
143,842 - -2.5
143,843 + -2.1
143,843 + -2.6
143,843 + -3.5
143,846 - -1.5
143,847 + -4.0
143,860 - -2.9
143,860 - -1.8
143,860 - -2.8
143,861 + -3.6
143,861 + -3.1
143,868 - -2.3
143,915 - -2.7
144,007 - -1.4
144,007 - -3.7
144,016 + -3.9
144,016 + -4.3
144,064 + -2.2
144,131 - -1.8
144,253 - -2.0
144,253 - -1.9
144,254 + -2.7
144,406 + -0.4
144,511 + +0.9
144,537 - +0.9
144,537 - -0.5
144,709 - -0.3
144,899 - -0.1
144,960 + +0.3
145,061 - -0.3
145,066 - +0.7
145,066 - +0.4
145,105 - +0.5

Or see this region's nucleotide sequence