Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1536 and BT1537 are separated by 243 nucleotides BT1537 and BT1538 are separated by 54 nucleotides
BT1536: BT1536 - ABC transporter permease (NCBI ptt file), at 1,895,063 to 1,896,310
BT1536
BT1537: BT1537 - putative NADH dehydrogenase (NCBI ptt file), at 1,896,554 to 1,897,945
BT1537
BT1538: BT1538 - hemagglutinin (NCBI ptt file), at 1,898,000 to 1,898,905
BT1538
Position (kb)
1896
1897
1898 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1895.580 kb on + strand, within BT1536 at 1895.585 kb on - strand, within BT1536 at 1895.720 kb on - strand, within BT1536 at 1895.720 kb on - strand, within BT1536 at 1895.763 kb on + strand, within BT1536 at 1895.764 kb on - strand, within BT1536 at 1895.820 kb on + strand, within BT1536 at 1895.876 kb on - strand, within BT1536 at 1896.050 kb on + strand, within BT1536 at 1896.205 kb on + strand at 1896.205 kb on + strand at 1896.206 kb on - strand at 1896.209 kb on + strand at 1896.314 kb on + strand at 1896.357 kb on + strand at 1896.357 kb on + strand at 1896.474 kb on - strand at 1896.474 kb on - strand at 1896.475 kb on + strand at 1896.475 kb on + strand at 1896.475 kb on + strand at 1896.476 kb on - strand at 1896.509 kb on - strand at 1896.561 kb on - strand at 1896.635 kb on - strand at 1896.684 kb on + strand at 1896.720 kb on + strand, within BT1537 at 1896.749 kb on + strand, within BT1537 at 1896.789 kb on - strand, within BT1537 at 1897.025 kb on + strand, within BT1537 at 1897.043 kb on + strand, within BT1537 at 1897.159 kb on + strand, within BT1537 at 1897.165 kb on + strand, within BT1537 at 1897.339 kb on + strand, within BT1537 at 1897.339 kb on + strand, within BT1537 at 1897.340 kb on - strand, within BT1537 at 1897.340 kb on - strand, within BT1537 at 1897.443 kb on - strand, within BT1537 at 1897.537 kb on + strand, within BT1537 at 1897.580 kb on - strand, within BT1537 at 1897.586 kb on - strand, within BT1537 at 1897.629 kb on + strand, within BT1537 at 1897.630 kb on - strand, within BT1537 at 1897.692 kb on - strand, within BT1537 at 1897.710 kb on + strand, within BT1537 at 1897.771 kb on + strand, within BT1537 at 1897.825 kb on + strand at 1897.826 kb on - strand at 1897.933 kb on + strand at 1897.961 kb on + strand at 1897.967 kb on - strand at 1897.984 kb on + strand at 1897.984 kb on + strand at 1898.001 kb on + strand at 1898.111 kb on - strand, within BT1538 at 1898.133 kb on + strand, within BT1538 at 1898.186 kb on - strand, within BT1538 at 1898.914 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 1,895,580 + BT1536 0.41 -2.0 1,895,585 - BT1536 0.42 -1.6 1,895,720 - BT1536 0.53 -2.0 1,895,720 - BT1536 0.53 -1.6 1,895,763 + BT1536 0.56 -2.1 1,895,764 - BT1536 0.56 +0.0 1,895,820 + BT1536 0.61 -2.8 1,895,876 - BT1536 0.65 -2.0 1,896,050 + BT1536 0.79 +1.2 1,896,205 + -1.0 1,896,205 + -0.3 1,896,206 - -2.6 1,896,209 + -0.4 1,896,314 + -1.9 1,896,357 + -3.3 1,896,357 + -2.2 1,896,474 - -0.8 1,896,474 - -1.4 1,896,475 + -0.4 1,896,475 + -1.6 1,896,475 + -0.7 1,896,476 - -2.3 1,896,509 - +0.4 1,896,561 - +1.6 1,896,635 - -2.0 1,896,684 + -2.6 1,896,720 + BT1537 0.12 +0.0 1,896,749 + BT1537 0.14 +0.0 1,896,789 - BT1537 0.17 -2.0 1,897,025 + BT1537 0.34 -3.8 1,897,043 + BT1537 0.35 -2.5 1,897,159 + BT1537 0.43 -2.3 1,897,165 + BT1537 0.44 -1.6 1,897,339 + BT1537 0.56 -1.0 1,897,339 + BT1537 0.56 -2.0 1,897,340 - BT1537 0.56 -1.0 1,897,340 - BT1537 0.56 -4.7 1,897,443 - BT1537 0.64 -1.0 1,897,537 + BT1537 0.71 -3.2 1,897,580 - BT1537 0.74 -1.0 1,897,586 - BT1537 0.74 -1.8 1,897,629 + BT1537 0.77 -1.0 1,897,630 - BT1537 0.77 -1.0 1,897,692 - BT1537 0.82 -3.3 1,897,710 + BT1537 0.83 -1.5 1,897,771 + BT1537 0.87 -2.6 1,897,825 + +0.0 1,897,826 - +0.0 1,897,933 + +0.0 1,897,961 + -1.0 1,897,967 - -0.6 1,897,984 + -1.6 1,897,984 + -1.3 1,898,001 + +3.2 1,898,111 - BT1538 0.12 -1.0 1,898,133 + BT1538 0.15 -1.0 1,898,186 - BT1538 0.21 +0.0 1,898,914 - +0.0
Or see this region's nucleotide sequence