Experiment: BHIS with Chlorpromazine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0539 and BT0540 are separated by 766 nucleotides BT0540 and BT0541 are separated by 5 nucleotides
BT0539: BT0539 - putative Na+/sulphate transporter (NCBI ptt file), at 661,448 to 663,307
BT0539
BT0540: BT0540 - putative exopolyphosphatase (NCBI ptt file), at 664,074 to 664,991
BT0540
BT0541: BT0541 - Polyphosphate kinase (NCBI ptt file), at 664,997 to 667,114
BT0541
Position (kb) 664
665 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 663.109 kb on + strand, within BT0539 at 663.151 kb on + strand at 663.151 kb on + strand at 663.152 kb on - strand at 663.214 kb on + strand at 663.214 kb on + strand at 663.215 kb on - strand at 663.215 kb on - strand at 663.220 kb on + strand at 663.231 kb on + strand at 663.231 kb on + strand at 663.237 kb on + strand at 663.256 kb on + strand at 663.256 kb on + strand at 663.277 kb on - strand at 663.312 kb on + strand at 663.323 kb on + strand at 663.386 kb on - strand at 663.392 kb on - strand at 663.529 kb on - strand at 663.644 kb on + strand at 663.652 kb on - strand at 663.666 kb on + strand at 663.728 kb on - strand at 663.801 kb on + strand at 663.992 kb on - strand at 664.100 kb on + strand at 664.155 kb on - strand at 664.155 kb on - strand at 664.159 kb on - strand at 664.159 kb on - strand at 664.188 kb on - strand, within BT0540 at 664.251 kb on - strand, within BT0540 at 664.251 kb on - strand, within BT0540 at 664.263 kb on - strand, within BT0540 at 664.411 kb on + strand, within BT0540 at 664.429 kb on - strand, within BT0540 at 664.572 kb on + strand, within BT0540 at 664.582 kb on + strand, within BT0540 at 664.627 kb on - strand, within BT0540 at 664.687 kb on - strand, within BT0540 at 664.756 kb on - strand, within BT0540 at 664.765 kb on + strand, within BT0540 at 664.995 kb on + strand at 665.006 kb on - strand at 665.196 kb on - strand at 665.285 kb on - strand, within BT0541 at 665.351 kb on - strand, within BT0541 at 665.368 kb on + strand, within BT0541 at 665.420 kb on + strand, within BT0541 at 665.420 kb on + strand, within BT0541 at 665.484 kb on + strand, within BT0541 at 665.489 kb on - strand, within BT0541 at 665.489 kb on - strand, within BT0541 at 665.540 kb on + strand, within BT0541 at 665.636 kb on - strand, within BT0541 at 665.707 kb on + strand, within BT0541 at 665.708 kb on - strand, within BT0541 at 665.708 kb on - strand, within BT0541 at 665.708 kb on - strand, within BT0541 at 665.773 kb on + strand, within BT0541 at 665.773 kb on + strand, within BT0541 at 665.818 kb on + strand, within BT0541
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.2 mM remove 663,109 + BT0539 0.89 +1.0 663,151 + -2.0 663,151 + -1.0 663,152 - -2.4 663,214 + +0.6 663,214 + -1.4 663,215 - -3.6 663,215 - -1.0 663,220 + -2.8 663,231 + +0.6 663,231 + +0.8 663,237 + -0.3 663,256 + +0.4 663,256 + -1.0 663,277 - -0.4 663,312 + -2.0 663,323 + -1.6 663,386 - -1.0 663,392 - -1.9 663,529 - -2.2 663,644 + -2.4 663,652 - +1.0 663,666 + -0.0 663,728 - -0.0 663,801 + -0.9 663,992 - -0.0 664,100 + -1.6 664,155 - -2.8 664,155 - -0.4 664,159 - -0.0 664,159 - -1.0 664,188 - BT0540 0.12 +1.1 664,251 - BT0540 0.19 +1.0 664,251 - BT0540 0.19 -0.0 664,263 - BT0540 0.21 +1.2 664,411 + BT0540 0.37 +1.6 664,429 - BT0540 0.39 -1.6 664,572 + BT0540 0.54 +0.6 664,582 + BT0540 0.55 +0.7 664,627 - BT0540 0.60 -1.0 664,687 - BT0540 0.67 -1.4 664,756 - BT0540 0.74 +0.6 664,765 + BT0540 0.75 -0.0 664,995 + +0.4 665,006 - -2.4 665,196 - +0.6 665,285 - BT0541 0.14 +1.6 665,351 - BT0541 0.17 -0.4 665,368 + BT0541 0.18 -0.0 665,420 + BT0541 0.20 -1.0 665,420 + BT0541 0.20 -1.1 665,484 + BT0541 0.23 -2.4 665,489 - BT0541 0.23 -2.8 665,489 - BT0541 0.23 +2.6 665,540 + BT0541 0.26 -0.4 665,636 - BT0541 0.30 -0.0 665,707 + BT0541 0.34 -2.6 665,708 - BT0541 0.34 +0.3 665,708 - BT0541 0.34 +1.6 665,708 - BT0541 0.34 +3.0 665,773 + BT0541 0.37 -0.6 665,773 + BT0541 0.37 -0.8 665,818 + BT0541 0.39 -0.6
Or see this region's nucleotide sequence