Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3886 and Psyr_3887 are separated by 128 nucleotides Psyr_3887 and Psyr_3888 overlap by 4 nucleotides Psyr_3888 and Psyr_3889 are separated by 106 nucleotides
Psyr_3886: Psyr_3886 - methionyl-tRNA synthetase, at 4,626,531 to 4,628,579
_3886
Psyr_3887: Psyr_3887 - Electron transport complex, RnfABCDGE type, B subunit, at 4,628,708 to 4,629,583
_3887
Psyr_3888: Psyr_3888 - endonuclease III / DNA-(apurinic or apyrimidinic site) lyase, at 4,629,580 to 4,630,218
_3888
Psyr_3889: Psyr_3889 - conserved hypothetical protein, at 4,630,325 to 4,630,507
_3889
Position (kb) 4628
4629
4630 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 4628.320 kb on + strand, within Psyr_3886 at 4628.320 kb on + strand, within Psyr_3886 at 4628.320 kb on + strand, within Psyr_3886 at 4628.320 kb on + strand, within Psyr_3886 at 4628.577 kb on + strand at 4628.578 kb on - strand at 4628.578 kb on - strand at 4628.578 kb on - strand at 4628.756 kb on - strand at 4628.828 kb on - strand, within Psyr_3887 at 4628.989 kb on + strand, within Psyr_3887 at 4629.713 kb on + strand, within Psyr_3888 at 4629.713 kb on + strand, within Psyr_3888 at 4629.714 kb on - strand, within Psyr_3888 at 4629.714 kb on - strand, within Psyr_3888 at 4629.723 kb on + strand, within Psyr_3888 at 4629.772 kb on + strand, within Psyr_3888 at 4629.772 kb on + strand, within Psyr_3888 at 4629.772 kb on + strand, within Psyr_3888 at 4629.772 kb on + strand, within Psyr_3888 at 4629.772 kb on + strand, within Psyr_3888 at 4629.772 kb on + strand, within Psyr_3888 at 4629.772 kb on + strand, within Psyr_3888 at 4629.772 kb on + strand, within Psyr_3888 at 4629.772 kb on + strand, within Psyr_3888 at 4629.773 kb on - strand, within Psyr_3888 at 4629.773 kb on - strand, within Psyr_3888 at 4629.773 kb on - strand, within Psyr_3888 at 4629.773 kb on - strand, within Psyr_3888 at 4629.773 kb on - strand, within Psyr_3888 at 4629.773 kb on - strand, within Psyr_3888 at 4629.773 kb on - strand, within Psyr_3888 at 4629.785 kb on + strand, within Psyr_3888 at 4629.786 kb on - strand, within Psyr_3888 at 4629.786 kb on - strand, within Psyr_3888 at 4629.802 kb on + strand, within Psyr_3888 at 4629.802 kb on + strand, within Psyr_3888 at 4629.802 kb on + strand, within Psyr_3888 at 4629.803 kb on - strand, within Psyr_3888 at 4629.823 kb on + strand, within Psyr_3888 at 4629.823 kb on + strand, within Psyr_3888 at 4629.823 kb on + strand, within Psyr_3888 at 4629.824 kb on - strand, within Psyr_3888 at 4629.999 kb on + strand, within Psyr_3888 at 4629.999 kb on + strand, within Psyr_3888 at 4629.999 kb on + strand, within Psyr_3888 at 4629.999 kb on + strand, within Psyr_3888 at 4630.000 kb on - strand, within Psyr_3888 at 4630.000 kb on - strand, within Psyr_3888 at 4630.000 kb on - strand, within Psyr_3888 at 4630.000 kb on - strand, within Psyr_3888 at 4630.000 kb on - strand, within Psyr_3888 at 4630.133 kb on - strand, within Psyr_3888 at 4630.150 kb on - strand, within Psyr_3888 at 4630.173 kb on + strand at 4630.175 kb on + strand at 4630.175 kb on + strand at 4630.175 kb on + strand at 4630.175 kb on + strand at 4630.175 kb on + strand at 4630.176 kb on - strand at 4630.176 kb on - strand at 4630.176 kb on - strand at 4630.176 kb on - strand at 4630.176 kb on - strand at 4630.192 kb on + strand at 4630.192 kb on + strand at 4630.193 kb on - strand at 4630.193 kb on - strand at 4630.193 kb on - strand at 4630.193 kb on - strand at 4630.230 kb on + strand at 4630.230 kb on + strand at 4630.230 kb on + strand at 4630.230 kb on + strand at 4630.231 kb on - strand at 4630.231 kb on - strand at 4630.231 kb on - strand at 4630.231 kb on - strand at 4630.286 kb on + strand at 4630.286 kb on + strand at 4630.286 kb on + strand at 4630.286 kb on + strand at 4630.286 kb on + strand at 4630.286 kb on + strand at 4630.286 kb on + strand at 4630.287 kb on - strand at 4630.287 kb on - strand at 4630.287 kb on - strand at 4630.287 kb on - strand at 4630.287 kb on - strand at 4630.287 kb on - strand at 4630.287 kb on - strand at 4630.287 kb on - strand at 4630.353 kb on + strand, within Psyr_3889 at 4630.353 kb on + strand, within Psyr_3889 at 4630.353 kb on + strand, within Psyr_3889 at 4630.353 kb on + strand, within Psyr_3889 at 4630.354 kb on - strand, within Psyr_3889 at 4630.354 kb on - strand, within Psyr_3889 at 4630.490 kb on + strand at 4630.505 kb on + strand at 4630.580 kb on + strand at 4630.580 kb on + strand at 4630.580 kb on + strand at 4630.580 kb on + strand at 4630.581 kb on - strand at 4630.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 4,628,320 + Psyr_3886 0.87 -2.8 4,628,320 + Psyr_3886 0.87 -0.6 4,628,320 + Psyr_3886 0.87 -1.7 4,628,320 + Psyr_3886 0.87 -0.2 4,628,577 + -1.0 4,628,578 - +0.2 4,628,578 - +0.1 4,628,578 - +1.3 4,628,756 - +1.0 4,628,828 - Psyr_3887 0.14 -0.1 4,628,989 + Psyr_3887 0.32 +1.9 4,629,713 + Psyr_3888 0.21 +0.8 4,629,713 + Psyr_3888 0.21 -0.1 4,629,714 - Psyr_3888 0.21 -1.7 4,629,714 - Psyr_3888 0.21 -2.1 4,629,723 + Psyr_3888 0.22 -0.5 4,629,772 + Psyr_3888 0.30 +1.3 4,629,772 + Psyr_3888 0.30 -1.7 4,629,772 + Psyr_3888 0.30 +0.2 4,629,772 + Psyr_3888 0.30 -3.5 4,629,772 + Psyr_3888 0.30 +1.3 4,629,772 + Psyr_3888 0.30 -0.3 4,629,772 + Psyr_3888 0.30 -0.1 4,629,772 + Psyr_3888 0.30 -1.7 4,629,772 + Psyr_3888 0.30 +2.1 4,629,773 - Psyr_3888 0.30 -2.4 4,629,773 - Psyr_3888 0.30 +1.1 4,629,773 - Psyr_3888 0.30 -0.6 4,629,773 - Psyr_3888 0.30 +1.1 4,629,773 - Psyr_3888 0.30 -2.8 4,629,773 - Psyr_3888 0.30 +0.3 4,629,773 - Psyr_3888 0.30 -1.7 4,629,785 + Psyr_3888 0.32 +1.3 4,629,786 - Psyr_3888 0.32 -0.4 4,629,786 - Psyr_3888 0.32 +0.2 4,629,802 + Psyr_3888 0.35 -0.6 4,629,802 + Psyr_3888 0.35 -0.3 4,629,802 + Psyr_3888 0.35 +0.3 4,629,803 - Psyr_3888 0.35 -0.4 4,629,823 + Psyr_3888 0.38 -0.6 4,629,823 + Psyr_3888 0.38 -0.7 4,629,823 + Psyr_3888 0.38 -1.6 4,629,824 - Psyr_3888 0.38 -0.5 4,629,999 + Psyr_3888 0.66 +1.6 4,629,999 + Psyr_3888 0.66 +1.3 4,629,999 + Psyr_3888 0.66 +0.1 4,629,999 + Psyr_3888 0.66 -0.2 4,630,000 - Psyr_3888 0.66 +0.1 4,630,000 - Psyr_3888 0.66 -2.0 4,630,000 - Psyr_3888 0.66 +4.1 4,630,000 - Psyr_3888 0.66 +0.6 4,630,000 - Psyr_3888 0.66 +2.5 4,630,133 - Psyr_3888 0.87 +0.1 4,630,150 - Psyr_3888 0.89 +0.4 4,630,173 + +0.4 4,630,175 + -0.8 4,630,175 + +1.4 4,630,175 + +0.0 4,630,175 + -1.9 4,630,175 + +0.8 4,630,176 - +1.4 4,630,176 - -2.1 4,630,176 - +0.7 4,630,176 - -0.9 4,630,176 - +0.5 4,630,192 + -0.8 4,630,192 + -1.7 4,630,193 - -1.1 4,630,193 - +0.8 4,630,193 - +0.3 4,630,193 - -1.5 4,630,230 + +1.4 4,630,230 + -0.1 4,630,230 + -0.1 4,630,230 + -0.3 4,630,231 - -0.2 4,630,231 - -0.1 4,630,231 - -0.9 4,630,231 - +0.2 4,630,286 + -1.7 4,630,286 + -0.4 4,630,286 + +2.2 4,630,286 + +0.4 4,630,286 + +1.1 4,630,286 + +0.4 4,630,286 + -0.1 4,630,287 - -1.6 4,630,287 - -0.7 4,630,287 - +1.1 4,630,287 - -1.2 4,630,287 - +0.8 4,630,287 - +2.0 4,630,287 - -2.8 4,630,287 - +0.4 4,630,353 + Psyr_3889 0.15 -0.5 4,630,353 + Psyr_3889 0.15 +1.4 4,630,353 + Psyr_3889 0.15 -4.0 4,630,353 + Psyr_3889 0.15 -2.5 4,630,354 - Psyr_3889 0.16 -0.5 4,630,354 - Psyr_3889 0.16 +0.9 4,630,490 + +0.0 4,630,505 + +0.6 4,630,580 + -0.3 4,630,580 + +0.7 4,630,580 + -1.2 4,630,580 + -0.5 4,630,581 - -1.7 4,630,581 - -2.5
Or see this region's nucleotide sequence