Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3454 and Psyr_3455 overlap by 11 nucleotides Psyr_3455 and Psyr_3456 are separated by 15 nucleotides Psyr_3456 and Psyr_3457 overlap by 8 nucleotides
Psyr_3454: Psyr_3454 - ATPase FliI/YscN, at 4,116,887 to 4,118,245
_3454
Psyr_3455: Psyr_3455 - Flagellar assembly protein FliH, at 4,118,235 to 4,119,053
_3455
Psyr_3456: Psyr_3456 - Flagellar motor switch protein FliG, at 4,119,069 to 4,120,085
_3456
Psyr_3457: Psyr_3457 - Flagellar FliF M-ring protein, at 4,120,078 to 4,121,862
_3457
Position (kb) 4119
4120
4121 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4118.202 kb on + strand at 4118.202 kb on + strand at 4118.202 kb on + strand at 4118.203 kb on - strand at 4118.203 kb on - strand at 4118.203 kb on - strand at 4118.414 kb on + strand, within Psyr_3455 at 4118.415 kb on - strand, within Psyr_3455 at 4118.565 kb on + strand, within Psyr_3455 at 4118.566 kb on - strand, within Psyr_3455 at 4118.659 kb on + strand, within Psyr_3455 at 4118.659 kb on + strand, within Psyr_3455 at 4118.659 kb on + strand, within Psyr_3455 at 4118.660 kb on - strand, within Psyr_3455 at 4118.660 kb on - strand, within Psyr_3455 at 4118.660 kb on - strand, within Psyr_3455 at 4118.781 kb on + strand, within Psyr_3455 at 4118.781 kb on + strand, within Psyr_3455 at 4118.782 kb on - strand, within Psyr_3455 at 4119.070 kb on + strand at 4119.070 kb on + strand at 4119.071 kb on - strand at 4119.103 kb on - strand at 4119.125 kb on + strand at 4119.126 kb on - strand at 4119.288 kb on + strand, within Psyr_3456 at 4119.306 kb on + strand, within Psyr_3456 at 4119.307 kb on - strand, within Psyr_3456 at 4119.307 kb on - strand, within Psyr_3456 at 4119.307 kb on - strand, within Psyr_3456 at 4119.307 kb on - strand, within Psyr_3456 at 4119.570 kb on + strand, within Psyr_3456 at 4119.571 kb on - strand, within Psyr_3456 at 4119.665 kb on - strand, within Psyr_3456 at 4119.761 kb on + strand, within Psyr_3456 at 4119.762 kb on - strand, within Psyr_3456 at 4119.771 kb on + strand, within Psyr_3456 at 4119.772 kb on - strand, within Psyr_3456 at 4119.772 kb on - strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.841 kb on + strand, within Psyr_3456 at 4119.842 kb on - strand, within Psyr_3456 at 4119.842 kb on - strand, within Psyr_3456 at 4119.842 kb on - strand, within Psyr_3456 at 4119.842 kb on - strand, within Psyr_3456 at 4119.842 kb on - strand, within Psyr_3456 at 4120.033 kb on - strand at 4120.172 kb on + strand at 4120.173 kb on - strand at 4120.200 kb on + strand at 4120.200 kb on + strand at 4120.200 kb on + strand at 4120.200 kb on + strand at 4120.201 kb on - strand at 4120.222 kb on + strand at 4120.342 kb on + strand, within Psyr_3457 at 4120.343 kb on - strand, within Psyr_3457 at 4120.418 kb on + strand, within Psyr_3457 at 4120.418 kb on + strand, within Psyr_3457 at 4120.418 kb on + strand, within Psyr_3457 at 4120.418 kb on + strand, within Psyr_3457 at 4121.031 kb on - strand, within Psyr_3457
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 4,118,202 + -0.7 4,118,202 + +0.6 4,118,202 + -0.1 4,118,203 - -1.5 4,118,203 - -0.1 4,118,203 - +0.3 4,118,414 + Psyr_3455 0.22 -0.1 4,118,415 - Psyr_3455 0.22 +1.3 4,118,565 + Psyr_3455 0.40 -0.9 4,118,566 - Psyr_3455 0.40 +0.7 4,118,659 + Psyr_3455 0.52 -0.2 4,118,659 + Psyr_3455 0.52 -2.3 4,118,659 + Psyr_3455 0.52 -1.1 4,118,660 - Psyr_3455 0.52 -1.2 4,118,660 - Psyr_3455 0.52 -1.8 4,118,660 - Psyr_3455 0.52 +0.3 4,118,781 + Psyr_3455 0.67 -0.6 4,118,781 + Psyr_3455 0.67 -1.0 4,118,782 - Psyr_3455 0.67 +1.4 4,119,070 + -2.0 4,119,070 + -1.1 4,119,071 - -0.6 4,119,103 - +0.0 4,119,125 + +2.6 4,119,126 - +1.7 4,119,288 + Psyr_3456 0.22 -2.4 4,119,306 + Psyr_3456 0.23 +0.8 4,119,307 - Psyr_3456 0.23 +1.3 4,119,307 - Psyr_3456 0.23 -0.1 4,119,307 - Psyr_3456 0.23 +1.5 4,119,307 - Psyr_3456 0.23 +1.8 4,119,570 + Psyr_3456 0.49 +0.8 4,119,571 - Psyr_3456 0.49 -0.6 4,119,665 - Psyr_3456 0.59 +0.7 4,119,761 + Psyr_3456 0.68 +0.4 4,119,762 - Psyr_3456 0.68 -0.7 4,119,771 + Psyr_3456 0.69 -2.5 4,119,772 - Psyr_3456 0.69 +0.5 4,119,772 - Psyr_3456 0.69 +1.2 4,119,841 + Psyr_3456 0.76 +0.5 4,119,841 + Psyr_3456 0.76 +0.3 4,119,841 + Psyr_3456 0.76 -0.7 4,119,841 + Psyr_3456 0.76 +0.8 4,119,841 + Psyr_3456 0.76 +0.0 4,119,841 + Psyr_3456 0.76 +2.7 4,119,841 + Psyr_3456 0.76 -1.0 4,119,842 - Psyr_3456 0.76 +0.4 4,119,842 - Psyr_3456 0.76 -0.3 4,119,842 - Psyr_3456 0.76 +0.7 4,119,842 - Psyr_3456 0.76 +0.8 4,119,842 - Psyr_3456 0.76 +1.0 4,120,033 - +1.1 4,120,172 + +1.4 4,120,173 - -0.3 4,120,200 + +0.4 4,120,200 + -1.3 4,120,200 + +0.7 4,120,200 + -1.5 4,120,201 - +0.9 4,120,222 + +1.2 4,120,342 + Psyr_3457 0.15 -0.2 4,120,343 - Psyr_3457 0.15 +3.0 4,120,418 + Psyr_3457 0.19 -1.0 4,120,418 + Psyr_3457 0.19 +0.7 4,120,418 + Psyr_3457 0.19 +0.9 4,120,418 + Psyr_3457 0.19 -0.9 4,121,031 - Psyr_3457 0.53 +1.2
Or see this region's nucleotide sequence