Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2999 and Psyr_3000 overlap by 4 nucleotides Psyr_3000 and Psyr_3001 overlap by 32 nucleotides Psyr_3001 and Psyr_3002 are separated by 9 nucleotides
Psyr_2999: Psyr_2999 - LmbE-like protein, at 3,595,728 to 3,596,501
_2999
Psyr_3000: Psyr_3000 - methyltransferase, putative, at 3,596,498 to 3,597,124
_3000
Psyr_3001: Psyr_3001 - Glycosyl transferase, family 2, at 3,597,093 to 3,597,746
_3001
Psyr_3002: Psyr_3002 - conserved hypothetical protein, at 3,597,756 to 3,598,796
_3002
Position (kb)
3597
3598 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3596.375 kb on + strand, within Psyr_2999 at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.517 kb on - strand at 3596.517 kb on - strand at 3596.573 kb on + strand, within Psyr_3000 at 3596.843 kb on + strand, within Psyr_3000 at 3596.847 kb on - strand, within Psyr_3000 at 3597.134 kb on - strand at 3597.134 kb on - strand at 3597.134 kb on - strand at 3597.359 kb on + strand, within Psyr_3001 at 3597.489 kb on + strand, within Psyr_3001 at 3597.490 kb on - strand, within Psyr_3001 at 3597.564 kb on + strand, within Psyr_3001 at 3597.564 kb on + strand, within Psyr_3001 at 3597.564 kb on + strand, within Psyr_3001 at 3597.564 kb on + strand, within Psyr_3001 at 3597.565 kb on - strand, within Psyr_3001 at 3597.565 kb on - strand, within Psyr_3001 at 3597.758 kb on - strand at 3597.964 kb on - strand, within Psyr_3002 at 3597.970 kb on + strand, within Psyr_3002 at 3598.051 kb on + strand, within Psyr_3002 at 3598.051 kb on + strand, within Psyr_3002 at 3598.051 kb on + strand, within Psyr_3002 at 3598.051 kb on + strand at 3598.052 kb on - strand, within Psyr_3002 at 3598.232 kb on - strand, within Psyr_3002 at 3598.329 kb on + strand, within Psyr_3002 at 3598.329 kb on + strand, within Psyr_3002 at 3598.329 kb on + strand, within Psyr_3002 at 3598.329 kb on + strand, within Psyr_3002 at 3598.329 kb on + strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.330 kb on - strand, within Psyr_3002 at 3598.400 kb on - strand, within Psyr_3002 at 3598.575 kb on - strand, within Psyr_3002
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 3,596,375 + Psyr_2999 0.84 -3.0 3,596,516 + -1.6 3,596,516 + -0.5 3,596,516 + +1.3 3,596,516 + +1.5 3,596,516 + -1.1 3,596,516 + -1.5 3,596,517 - -1.6 3,596,517 - -0.2 3,596,573 + Psyr_3000 0.12 +0.4 3,596,843 + Psyr_3000 0.55 -1.3 3,596,847 - Psyr_3000 0.56 +0.3 3,597,134 - +0.8 3,597,134 - +1.9 3,597,134 - +1.6 3,597,359 + Psyr_3001 0.41 +1.5 3,597,489 + Psyr_3001 0.61 -2.1 3,597,490 - Psyr_3001 0.61 +1.1 3,597,564 + Psyr_3001 0.72 +0.9 3,597,564 + Psyr_3001 0.72 -3.0 3,597,564 + Psyr_3001 0.72 -2.5 3,597,564 + Psyr_3001 0.72 -0.2 3,597,565 - Psyr_3001 0.72 -1.1 3,597,565 - Psyr_3001 0.72 +0.4 3,597,758 - +3.5 3,597,964 - Psyr_3002 0.20 +0.3 3,597,970 + Psyr_3002 0.21 -1.4 3,598,051 + Psyr_3002 0.28 +0.8 3,598,051 + Psyr_3002 0.28 +0.6 3,598,051 + Psyr_3002 0.28 -0.2 3,598,051 + +2.2 3,598,052 - Psyr_3002 0.28 -0.4 3,598,232 - Psyr_3002 0.46 -2.3 3,598,329 + Psyr_3002 0.55 -0.3 3,598,329 + Psyr_3002 0.55 +1.0 3,598,329 + Psyr_3002 0.55 -2.0 3,598,329 + Psyr_3002 0.55 -1.6 3,598,329 + Psyr_3002 0.55 -1.4 3,598,330 - Psyr_3002 0.55 -0.1 3,598,330 - Psyr_3002 0.55 -0.1 3,598,330 - Psyr_3002 0.55 -1.4 3,598,330 - Psyr_3002 0.55 -0.3 3,598,400 - Psyr_3002 0.62 -0.8 3,598,575 - Psyr_3002 0.79 -0.3
Or see this region's nucleotide sequence