Experiment: KB with Plumbagin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2986 and Psyr_2987 are separated by 51 nucleotides Psyr_2987 and Psyr_2988 overlap by 8 nucleotides Psyr_2988 and Psyr_2989 overlap by 1 nucleotides
Psyr_2986: Psyr_2986 - Glycoside hydrolase, family 15, at 3,576,736 to 3,578,553
_2986
Psyr_2987: Psyr_2987 - Cof protein:HAD-superfamily hydrolase, subfamily IIB, at 3,578,605 to 3,579,414
_2987
Psyr_2988: Psyr_2988 - glucose-6-phosphate 1-dehydrogenase, at 3,579,407 to 3,580,939
_2988
Psyr_2989: Psyr_2989 - 6-phosphogluconate dehydrogenase (decarboxylating), at 3,580,939 to 3,581,913
_2989
Position (kb)
3579
3580
3581 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3578.531 kb on + strand at 3578.531 kb on + strand at 3578.621 kb on + strand at 3578.621 kb on + strand at 3578.661 kb on + strand at 3578.668 kb on - strand at 3578.801 kb on + strand, within Psyr_2987 at 3578.801 kb on + strand, within Psyr_2987 at 3578.861 kb on + strand, within Psyr_2987 at 3578.865 kb on - strand, within Psyr_2987 at 3578.985 kb on - strand, within Psyr_2987 at 3579.011 kb on + strand, within Psyr_2987 at 3579.011 kb on + strand, within Psyr_2987 at 3579.011 kb on + strand, within Psyr_2987 at 3579.011 kb on + strand, within Psyr_2987 at 3579.011 kb on + strand, within Psyr_2987 at 3579.012 kb on - strand, within Psyr_2987 at 3579.012 kb on - strand, within Psyr_2987 at 3579.012 kb on - strand, within Psyr_2987 at 3579.047 kb on + strand, within Psyr_2987 at 3579.397 kb on + strand at 3579.397 kb on + strand at 3579.397 kb on + strand at 3579.398 kb on - strand at 3579.490 kb on - strand at 3579.490 kb on - strand at 3579.606 kb on + strand, within Psyr_2988 at 3579.606 kb on + strand, within Psyr_2988 at 3579.607 kb on - strand, within Psyr_2988 at 3579.607 kb on - strand, within Psyr_2988 at 3579.607 kb on - strand, within Psyr_2988 at 3579.607 kb on - strand, within Psyr_2988 at 3579.658 kb on - strand, within Psyr_2988 at 3579.658 kb on - strand, within Psyr_2988 at 3579.658 kb on - strand, within Psyr_2988 at 3579.763 kb on - strand, within Psyr_2988 at 3579.763 kb on - strand, within Psyr_2988 at 3579.856 kb on + strand, within Psyr_2988 at 3579.856 kb on + strand, within Psyr_2988 at 3579.857 kb on - strand, within Psyr_2988 at 3579.857 kb on - strand, within Psyr_2988 at 3579.857 kb on - strand, within Psyr_2988 at 3579.857 kb on - strand, within Psyr_2988 at 3579.857 kb on - strand, within Psyr_2988 at 3579.930 kb on + strand, within Psyr_2988 at 3579.930 kb on + strand, within Psyr_2988 at 3579.930 kb on + strand, within Psyr_2988 at 3579.930 kb on + strand, within Psyr_2988 at 3579.931 kb on - strand, within Psyr_2988 at 3579.931 kb on - strand, within Psyr_2988 at 3579.931 kb on - strand, within Psyr_2988 at 3579.931 kb on - strand, within Psyr_2988 at 3579.931 kb on - strand, within Psyr_2988 at 3579.931 kb on - strand, within Psyr_2988 at 3580.015 kb on - strand, within Psyr_2988 at 3580.226 kb on + strand, within Psyr_2988 at 3580.263 kb on + strand, within Psyr_2988 at 3580.263 kb on + strand, within Psyr_2988 at 3580.263 kb on + strand, within Psyr_2988 at 3580.264 kb on - strand, within Psyr_2988 at 3580.264 kb on - strand, within Psyr_2988 at 3580.596 kb on + strand, within Psyr_2988 at 3580.597 kb on - strand, within Psyr_2988 at 3580.597 kb on - strand, within Psyr_2988 at 3580.629 kb on + strand, within Psyr_2988 at 3580.629 kb on + strand, within Psyr_2988 at 3580.629 kb on + strand, within Psyr_2988 at 3580.630 kb on - strand, within Psyr_2988 at 3580.630 kb on - strand, within Psyr_2988 at 3580.671 kb on - strand, within Psyr_2988 at 3580.824 kb on + strand at 3580.940 kb on + strand at 3580.940 kb on + strand at 3580.940 kb on + strand at 3581.387 kb on + strand, within Psyr_2989 at 3581.387 kb on + strand, within Psyr_2989 at 3581.387 kb on + strand, within Psyr_2989 at 3581.387 kb on + strand, within Psyr_2989 at 3581.523 kb on - strand, within Psyr_2989 at 3581.523 kb on - strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.621 kb on + strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.828 kb on + strand at 3581.829 kb on - strand at 3581.829 kb on - strand at 3581.926 kb on + strand at 3581.926 kb on + strand at 3581.927 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Plumbagin 10 mM remove 3,578,531 + +1.7 3,578,531 + +0.9 3,578,621 + -0.8 3,578,621 + +0.9 3,578,661 + +0.5 3,578,668 - -0.1 3,578,801 + Psyr_2987 0.24 -1.6 3,578,801 + Psyr_2987 0.24 +0.2 3,578,861 + Psyr_2987 0.32 +0.7 3,578,865 - Psyr_2987 0.32 +0.2 3,578,985 - Psyr_2987 0.47 +0.1 3,579,011 + Psyr_2987 0.50 -0.2 3,579,011 + Psyr_2987 0.50 +1.6 3,579,011 + Psyr_2987 0.50 +0.7 3,579,011 + Psyr_2987 0.50 +1.2 3,579,011 + Psyr_2987 0.50 -1.1 3,579,012 - Psyr_2987 0.50 +0.3 3,579,012 - Psyr_2987 0.50 -0.1 3,579,012 - Psyr_2987 0.50 -1.1 3,579,047 + Psyr_2987 0.55 -2.0 3,579,397 + +1.9 3,579,397 + +0.0 3,579,397 + +0.1 3,579,398 - -2.1 3,579,490 - -0.9 3,579,490 - +1.5 3,579,606 + Psyr_2988 0.13 +0.4 3,579,606 + Psyr_2988 0.13 -0.5 3,579,607 - Psyr_2988 0.13 -3.5 3,579,607 - Psyr_2988 0.13 +1.2 3,579,607 - Psyr_2988 0.13 -1.6 3,579,607 - Psyr_2988 0.13 -3.3 3,579,658 - Psyr_2988 0.16 -2.3 3,579,658 - Psyr_2988 0.16 -0.7 3,579,658 - Psyr_2988 0.16 +0.1 3,579,763 - Psyr_2988 0.23 +0.7 3,579,763 - Psyr_2988 0.23 -1.3 3,579,856 + Psyr_2988 0.29 -1.0 3,579,856 + Psyr_2988 0.29 +0.8 3,579,857 - Psyr_2988 0.29 +0.8 3,579,857 - Psyr_2988 0.29 +0.6 3,579,857 - Psyr_2988 0.29 -1.3 3,579,857 - Psyr_2988 0.29 -0.3 3,579,857 - Psyr_2988 0.29 -0.8 3,579,930 + Psyr_2988 0.34 -2.8 3,579,930 + Psyr_2988 0.34 -0.6 3,579,930 + Psyr_2988 0.34 +0.6 3,579,930 + Psyr_2988 0.34 +0.7 3,579,931 - Psyr_2988 0.34 -0.2 3,579,931 - Psyr_2988 0.34 -0.6 3,579,931 - Psyr_2988 0.34 -2.3 3,579,931 - Psyr_2988 0.34 +0.1 3,579,931 - Psyr_2988 0.34 +1.1 3,579,931 - Psyr_2988 0.34 +1.2 3,580,015 - Psyr_2988 0.40 -0.3 3,580,226 + Psyr_2988 0.53 -0.2 3,580,263 + Psyr_2988 0.56 +0.7 3,580,263 + Psyr_2988 0.56 -1.6 3,580,263 + Psyr_2988 0.56 -2.3 3,580,264 - Psyr_2988 0.56 -0.3 3,580,264 - Psyr_2988 0.56 +1.0 3,580,596 + Psyr_2988 0.78 -1.9 3,580,597 - Psyr_2988 0.78 +1.2 3,580,597 - Psyr_2988 0.78 +0.8 3,580,629 + Psyr_2988 0.80 -0.9 3,580,629 + Psyr_2988 0.80 +0.4 3,580,629 + Psyr_2988 0.80 -0.9 3,580,630 - Psyr_2988 0.80 +0.5 3,580,630 - Psyr_2988 0.80 +1.1 3,580,671 - Psyr_2988 0.82 -0.6 3,580,824 + -0.1 3,580,940 + -0.8 3,580,940 + -0.1 3,580,940 + -0.8 3,581,387 + Psyr_2989 0.46 -3.8 3,581,387 + Psyr_2989 0.46 -1.7 3,581,387 + Psyr_2989 0.46 -2.2 3,581,387 + Psyr_2989 0.46 -3.4 3,581,523 - Psyr_2989 0.60 -1.6 3,581,523 - Psyr_2989 0.60 -3.0 3,581,570 + Psyr_2989 0.65 +2.8 3,581,570 + Psyr_2989 0.65 -1.0 3,581,570 + Psyr_2989 0.65 -3.6 3,581,570 + Psyr_2989 0.65 -1.3 3,581,571 - Psyr_2989 0.65 -1.4 3,581,571 - Psyr_2989 0.65 -0.4 3,581,571 - Psyr_2989 0.65 +0.8 3,581,571 - Psyr_2989 0.65 -1.8 3,581,621 + Psyr_2989 0.70 -0.6 3,581,622 - Psyr_2989 0.70 -1.1 3,581,622 - Psyr_2989 0.70 -2.9 3,581,622 - Psyr_2989 0.70 -0.1 3,581,622 - Psyr_2989 0.70 -0.2 3,581,828 + -1.9 3,581,829 - -0.4 3,581,829 - -3.2 3,581,926 + -0.2 3,581,926 + -2.5 3,581,927 - +1.2
Or see this region's nucleotide sequence